Detection of a human chromosomal translocation t(8;9) in a baby with multiple malformations using two-color fluorescence in situ hybridization

A human chromosomal translocation t(8;9) was detected using two-color fluorescence in situ hybridization with probes capable of staining the entire lengths of each of these chromosomes. The chromosome 8 probe was labeled with biotin and detected with Texas red, while the chromosome 9 probe was labeled with AAF and detected with FITC . In normal metaphase spreads, two metaphases from the proband, two red, one green and one part red and part green derivative chromosome were seen. The bicolor chromosome corresponded to translocation of a chromosome 8 segment to the distal part of the q region of one chromosome 9, as originally indicated by banding analysis. In interphase nuclei of the proband, four domains with bright fluorescence were recognized in many nuclei. Two were red, one was green, and the fourth had portions of both colors, indicating the presence of the translocation.     

HBV DNA及HBxDNA亚片段在肝癌细胞内的整合及作用

为探讨ＨＢＶＤＮＡ及其亚基因片段ＨＢｘＤＮ在肝细胞肝癌（ＨＣＣ）中整合特点用机制，制备了地高辛标记的３．２ｋｂＨＢＶＤＮＡ全基因及０．５９ｋｂＨＢＶＤＮＡ／ＢａｍＨＩ，ＢｇｌⅡＢＨｘＤＮＡ亚基因探针。点杂交、原位杂交及Ｓｏｕｔｈｅｒｎ杂交检测ＨＢＶＤＮＡ的存在情况，筛选ＨＢＶＤＮＡ纯整合标本，以ＨＢｘＤＮＡ探针检测ＨＢｘＤＮＡ在ＨＣＣ染色体的整合。结果显示ＨＢＶＤＮＡ在肝细胞内以核型为主（７０％）     

新一代低频电治疗仪的研制

本文介绍一种新型低频电脉冲治疗仪，采用新型单片机、程序控制和OTL功率放大器，产生变化有序的多种治疗脉冲波形模拟针灸治疗。电脉冲刺激器治疗仪可以用作减轻疼痛、放松肌肉、预防和减缓肌肉萎缩、增加局部血液循环等。     

人毛乳头细胞凝集性生长差异表达基因cDNA文库的构建

目的 构建毛乳头细胞凝集性生长状态下差异表达基因的消减cDNA文库。方法 分别提取凝集性生长和非凝集性生长状态下毛乳头细胞中的总RNA，应用SMART cDNA合成技术和抑制性消减杂交技术分离毛乳头细胞凝集性生长状态下差异表达基因的cDNA片段并建立消减cDNA文库；利用PCR对随机挑选的100个白色菌落进行插入片段的验证，对其中证实有插入片段的30个克隆进行cDNA斑点杂交验证。结果 所构建的消减cDNA文库扩增后得到320个阳性克隆，随机挑选的100个阳性克隆中95％的均有长度在100—600bp的插入片段，cDNA斑点杂交验证显示27个(90．0％)克隆为阳性。结论 结合SMART cDNA合成技术，应用抑制性消减杂交成功地构建了毛乳头细胞凝集性生长差异表达基因消减cDNA文库，为进一步筛选和克隆毛乳头细胞凝集性生长相关基因奠定了基础。     

大鼠脑震荡c-fos癌基因的表达及其法医病理学意义

目的 探讨原癌基因c-fos的表达和脑震荡性损伤的关系,同时寻找脑震荡后法医病理学的诊断依据。方法 55只实验大鼠随机分为脑震荡组和对照组。c-fos-mRNA表达采用原位杂交法观察。结果 对照组大鼠未见c-fos-mRNA的表达,然而脑震荡组在损伤后15min即可在神经元和胶质细胞观察到c-fos-mRNA的表达,至30min c-fos-mRNA的阳性反应达最高峰,以后逐渐下降。结论 c-fos原癌基因的检测可成为诊断脑震荡和推断脑震荡后经过时间的一项敏感指标。     

Molecular cytogenetic characterization of Thinopyrum and wheat--Thinopyrum translocated chromosomes in a wheat--Thinopyrum amphiploid

The wheat--Thinopyrum amphiploid 'Agrotriticum # 3425' (AT 3425), which is highly resistant to Cephalosporium stripe, was identified to carry seven pairs of Thinopyrum chromosomes, three pairs of wheat--Thinopyrum translocated chromosomes and 18 pairs of wheat chromosomes. Fluorescence genomic in situ hybridization (FGISH), C-banding, sequential C-banding and FGISH, and denaturing polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize and identify the chromosomes. The Thinopyrum chromosomes in AT 3425 were designated as T1 through T7 based on their C-banding patterns. The FGISH and C-banding patterns of mitotic chromosomes in AT 3425 and meiotic chromosomes in the hybrid between AT 3425 and wheat cultivar 'Chinese Spring' (CS) revealed that wheat chromosomes 1D, 2B and 3D were involved in the three wheat-Thinopyrum chromosome translocations designated as (W-T)1, (W-T)2, and (W-T)3 respectively. The analysis of high-molecular-weight glutenin subunits in single seeds of AT 3425 confirmed the involvement of wheat chromosome 1D in the translocation (W-T)1. The designations 1DSuu.1DL-1TL, 2BSuu.2BL-2TL and 3DSuu.3DL-3TL were suggested for the wheat--Thinopyrum translocated chromosomes (W-T)1, (W-T)2 and (W-T)3 in AT 3425 respectively.     

Comparative Analysis of Crossover Exchanges and Chiasmata in Allium Cepa × Fistulosum After Genomic In Situ Hybridization (GISH)

Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.