A COMPARATIVE STUDY OF GUT SUTURE ,HUMAN AMNION COLLAGEN,BOVINE SKIN COLLAGEN AND VICRYL SUTURE IMPLANTS IN RATS

This study compares the persistence and histological characteristics of gut suture with those of human amnion collagen ,bovine collagen ,and Vicryl suture implants in rats. Gut suture and human amnion collagen more resembled living tissue than did bovine collagen and were characterized by their cellularity and the presence of numerous capillaries. The Vicryl suture implants were quickly absorbed. Picrcalrius polarization revealed the synthesis of host collagen by rat fibroblasts which immigrated into the gut suture and human amnion collagen implants. The authors suggest the potential of gut suture as a soft tissue substitute to improve linear skin contour deficits.     

宜昌市依法规范婚前保健工作势在必行

婚前保健工作必须依法开展 ,而湖北省宜昌市城区婚前保健工作仍处于较混乱状态。经调查 ,存在着机构不合法 ,婚前医学检查质量欠保障 ,房屋、设备简陋 ,人员配置随意 ,法律意识淡薄等问题 ,致使婚前保健管理混乱。而部门配合不力 ,执法力度不够是导致混乱的重要因素之一。因此 ,严格准入制度 ,依法规范服务 ,将地市级城市婚前保健工作集中在市级妇幼保健机构是较佳选择 ,符合城镇卫生体制改革和区域规划的要求。各级领导重视 ,加大执法力度是依法规范婚前保健工作的保证 ;取得民政部门的配合是实现依法开展婚前保健工作的关键 ;广泛宣传 ,增强当事人的法律意识是提高婚前保健工作质量的重要措施。     

影响尿液检测结果的若干因素分析

了解影响尿液检验质量的因素,认识其对临床诊断的重要性,采取相应的措施和建议。采用大量实验结果和相关文献证实,尿液标本检验受多种因素(包括肉眼血尿、药物、尿液pH、标本放置时间、尿液试纸带)的影响。其结果直接影响尿液检验的各项指标,造成不同程度的误诊,对这些干扰因素的分析和排除在理论和实践上对临床诊断、治疗有很重要的意义。     

On the independence of P300 and the CNV: A short critique of the principal components analysis of Donchin et al. (1975)

Donchin, Tueting, Ritter, Kutas and Heffley (1975) present evidence from a principal components analysis (PCA) that the CNV and P300 are independent. This short critique points out a number of errors in their PCA and presents a reworking of their analysis. A number of further aspects of Donchin et al.'s paper are also discussed. The general value of this contribution in drawing the attention of EP researchers to potential sources of error in the application of factor analysis is emphasised.     

An evaluation of the stability of factor VIII inhibitors in plasma and plasma dried on filter paper discs stored at room temperature

The transportation of plasma specimens to specialized haemophilia centre laboratories for anti-factor VIII inhibitor titre determination is often necessary. The routine method of transporting frozen specimens on dry ice is limited by its cost and need for special handling. The present study was undertaken to evaluate the effect of storing specimens at room temperature on the FVIII inhibitor titre determinations using the Bethesda assay. Specimens stored both in liquid phase as well as adsorbed onto filter paper discs were studied. The results of the present study demonstrate that plasma specimens stored for up to 2 weeks at room temperature, either in liquid phase or adsorbed onto filter paper, yield equivalent measures of FVIII inhibitor titres using the Bethesda assay to plasma specimens stored frozen at -70 degrees C. Plasma specimens dried on filter paper discs and stored at room temperature offers a reliable, more practical and less expensive alternative to frozen plasma as a means of transport to specialized referral laboratories for analysis of anti-FVIII titres.     

滤纸干血滴间接荧光抗体试验(IFAT) 检测人群弓形体感染的研究

采用滤纸干血滴标本进行间接荧光抗体试验,对徐州市部分小学生、职业中学学生及肉联厂工人调查弓形体感染情况。在三个单位530名人群中,阳性137人,阳性率为25.85％。小学生阳性率为21.15％,职业中学学生为19.08％、肉联厂工人为33.78％、男性237人,阳性率为21.94％;女性293人,阳性率为29.01％;其中育龄妇女145人,阳性率为37.24％。滤纸干血滴浸液和血清标本进行间接荧光抗体试验相比较,结果一致。     

乳状液膜法处理草浆纸厂黑液：传质过程及膜组成研究

提出了一种新的草浆纸厂黑液处理方法－乳状液膜分离法，讨论了该方法的基本原理及传质过程，认为废水中的碱木质素以ＲＯＮａ形式选择性透过液膜，为Ｉ型促进迁移。测定了传质系数，考察了表面活性剂种类、浓度和内相酸浓度对ＣＯＤ去除率的影响，在本实验条件下，采用低电压破乳，废水中ＣＯＤ去除率可达９８％左右。     

Adherence evaluation of vented chest seals in a swine skin model

ObjectivesPerforation of the chest (open pneumothorax) with and without lung injury can cause air accumulation in the chest, positive intrapleural pressure and lead to tension pneumothorax if untreated. The performance of chest seals to prevent tension physiology depends partially on their ability to adhere to the skin and seal the chest wound. Novel non-occlusive vented chest seals were assessed for their adhesiveness on skin of live swine under normal and extreme environmental conditions to simulate austere battlefield conditions.MethodsChest seals were applied on the back of the swine on skin that was soiled by various environmental contaminants to represent battlefield situations. A peeling (horizontal rim peeling) and detachment and breaching (vertical pulling) techniques were used to quantify the adhesive performance of vented chest seals. Among eight initially selected vented seals, five (Bolin, Russell, Fast breathe, Hyfin and SAM) were further down-selected based on their superior adherence scores at ambient temperatures. The adherence of these seals was then assessed after approximately 17 h storage at extreme cold (−19.5 °C) and hot (71.5 °C) temperatures.ResultsAdherence scores for peeling (above 90%) and detachment scores (less than 25%) were comparable for four vented chest seals when tested at ambient temperature, except for the Bolin seal which had higher breaching. Under extreme storage temperatures, adherence peeling scores were comparable to those at ambient temperatures for four chest seals. Scores were significantly lower for the Bolin seal at extreme temperatures. This seal also had the highest detachment and breaching scores. In contrast, the Russell, Fast breathe, Hyfin and SAM seals showed similar ability to stay air tight without breaching after hot storage.ConclusionNo significant difference was found in skin adherence of the five vented chest seals at ambient temperature and the four seals (Russell, Fast breathe, Hyfin and SAM) maintained superior adherence even after exposure to extreme temperatures compared to the Bolin. To select the most effective product from the 5 selected vented chest seals, further functional evaluation of the valve of these chest seals on a chest wound with the potential for tension in the pneumothorax or hemopneumothorax is warranted.     

Participation of MicroRNA-34a and RANKL on bone repair induced by poly(vinylidene-trifluoroethylene)/barium titanate membrane

The poly(vinylidene-trifluoroethylene)/barium titanate (PVDF) membrane enhances in vitro osteoblast differentiation and in vivo bone repair. Here, we hypothesized that this higher bone repair could be also due to bone resorption inhibition mediated by a microRNA (miR)/RANKL circuit. To test our hypothesis, the large-scale miR expression of bone tissue grown on PVDF and polytetrafluoroethylene (PTFE) membranes was evaluated to identify potential RANKL-targeted miRs modulated by PVDF. The animal model used was rat calvarial defects implanted with either PVDF or PTFE. At 4 and 8 weeks, the bone tissue grown on membranes was submitted to a large-scale analysis of miRs by microarray. The expression of miR-34a and some of its targets, including RANKL, were evaluated by real-time polimerase chain reaction and osteoclast activity was detected by tartrate-resistant acid phosphatase (TRAP) staining. Among more than 250 miRs, twelve, including miR-34a, were simultaneously higher expressed (≥2 fold) at 4 and 8 weeks on PVDF. The higher expression of miR-34a was concomitant with a reduced expression of all its evaluated targets, including RANKL. Additionally, more TRAP-positive cells were observed in bone tissue grown on PTFE compared with PVDF in both time points. In conclusion, our results suggest that the higher bone formation induced by PVDF could be, at least in part, triggered by a miR-34a increase and RANKL decrease, which may inhibit osteoclast differentiation and activity, and bone resorption.     

Insight into the Unexpectedly Rapid Degradation of Dihydroxyacetone‐Based Hydrogels

Biodegradable hydrogels are an important class of biomaterials with a diverse range of applications. In some cases, a rapid hydrogel degradation rate is advantageous. Polycarbonate hydrogels based on dihydroxyacetone (DHA), a natural metabolite, have been reported to undergo surprisingly fast hydrolytic degradation. In the present work, insight into the key features of DHA that contribute to the observed degradation rates is gained. In vitro degradation (mass loss) of three different chemically cross‐linked polycarbonate hydrogels is investigated to shed light on the role of the ketone functional group, as well as the carbon‐chain length between the ketone and carbonate bonds. The ketone is found to be the major cause of rapid degradation. Also, mass loss is accelerated by increased temperature and pH, offering insight into potential tuning parameters and storage conditions. The results show that DHA is a promising monomeric unit for the design of rapidly degrading, biocompatible, and functional biomaterials.