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21.
Interneurons immunoreactive for vasoactive intestinal polypeptide (VIP) are integral elements of columnar organization patterns in the rat cerebral cortex. By application of the sensitive mirror technique, the co-localization of VIP with the classical inhibitory neurotransmitter γ-aminobutyric acid (GABA) and the acetylcholine-synthesizing enzyme, choline acetyltransferase (ChAT), was investigated in neocortical neurons. Furthermore, the frequency of co-localization of ChAT with GABA was determined. In a sample of 118 VIP-immunoreactive neurons, mostly from the primary somatosensory cortex, it was demonstrated that virtually all of them reveal immunoreactivity for GABA and, therefore, are to be GABAergic. Moreover, 34% of mostly bipolar, VIP-positive neurons contained ChAT and are, thus, supposedly cholinergic as well. Co-localization of VIP and ChAT varied according to cortical laminae. Finally, 88% of a total of 60 ChAT-immunoreactive neurons were also immunostained for GABA. It is concluded that almost all VIP-immunoreactive neurons and most of the cholinergic neurons in rat neocortex represent partly overlapping subpopulations of inhibitory interneurons utilizing GABA. 相似文献
22.
Role of intracortical mechanisms in the late part of the silent period to transcranial stimulation of the human motor cortex 总被引:3,自引:0,他引:3
J.P. Brasil-Neto A. Cammarota J. Valls-Solé A. Pascual-Leone M. Hallett L. G. Cohen 《Acta neurologica Scandinavica》1995,92(5):383-386
Transcranial magnetic stimulation (TMS) and transcranial electrical stimulation (TES) of the human motor cortex produce a silent period (SP) following motor evoked potentials (MEPs). The early part of the SP can be explained by decreased alpha motor neuron excitability, whereas the late part is presumably due to suprasegmental mechanisms. In order to determine the level of the suprasegmental contribution to the generation of SPs, we recorded excitatory and inhibitory responses to TMS, TES, and percutaneous electrical brainstem stimulation (PBS) in the voluntarily activated first dorsal interosseous muscle of the hand. Stimulus intensities were set so that PBS and TES induced MEPs with areas equal to or larger than those of MEPs obtained with TMS. This procedure revealed that SPs were 49% and 83% shorter with TES and PBS, respectively, than with TMS. As TMS is more effective than TES or PBS in activating cortical interneurons, these findings support the idea that a significant component of the SP arises from intracortical mechanisms. 相似文献
23.
Figure-Ground Segregation at Contours: a Neural Mechanism in the Visual Cortex of the Alert Monkey 总被引:1,自引:0,他引:1
An important task of vision is the segregation of figure and ground in situations of spatial occlusion. Psychophysical evidence suggests that the depth order at contours is defined early in visual processing. We have analysed this process in the visual cortex of the alert monkey. The animals were trained on a visual fixation task which reinforced foveal viewing. During periods of active visual fixation, we recorded the responses of single neurons in striate and prestriate cortex (areas V1, V2, and V3/V3A). The stimuli mimicked situations of spatial occlusion, usually a uniform light (or dark) rectangle overlaying a grating texture of opposite contrast. The direction of figure and ground at the borders of these rectangles was defined by the direction of the terminating grating lines (occlusion cues). Neuronal responses were analysed with respect to figure-ground direction and contrast polarity at such contours. Striate neurons often failed to respond to such stimuli, or were selective for contrast polarity; others were non-selective. Some neurons preferred a certain combination of figure-ground direction and contrast polarity. These neurons were rare both in striate and prestriate cortex. The majority of neurons signalled figure-ground direction independent of contrast polarity. These neurons were only found in prestriate cortex. We explain these responses in terms of a model which also explains neuronal signals of illusory contours. These results suggest that occlusion cues are used at an early level of processing to segregate figure and ground at contours. 相似文献
24.
加锌对大鼠脑内微量元素的影响 总被引:1,自引:0,他引:1
龚云 《微量元素与健康研究》2005,22(4):7-8
用SD雌性成年大鼠20只,随机分为实验组和对照组各10只。加锌40d后,用原子吸收分光光度计(日本津岛)分别测定了两组大鼠大脑皮质感觉运动区和海马CA3区的微量元素Zn、Cu、Fe和轻金属Mg的含量。结果表明:在大脑皮质感觉运动区,两组大鼠的诸元素含量无显著变化;在海马CA3区,两组大鼠的微量元素Zn、Cu、Fe的含量也无显著变化(P>0.05),但轻金属Mg的含量却发生了显著的变化,表现为对照组明显高于实验组(P<0.05)。 相似文献
25.
K. Dorovini-Zis A. P. Zis 《Journal of neural transmission (Vienna, Austria : 1996)》1991,84(1-2):75-84
Summary Autonomic innervation of the adrenal cortex has been demonstrated in several species. Detailed ultrastructural studies on the innervation of the zona fasciculata of the normal human adrenal cortex are lacking. We report herein our observations on the pattern of innervation of the cells of the zona fasciculata of the normal adult human cortex at both the light and electron microscope levels. Postganglionic unmyelinated fibers were observed to descend from a dense capsular meshwork and to be distributed as delicate branches among the columns of endocrine cells. Immunoperoxidase staining confirmed the presence of nerve fibers in the zona fasciculata in a distribution similar to that observed after staining with silver impregnation methods. Ultrastructural findings lent further support to these observations by the demonstration of bundles of unmyelinated fibers with focal enlargements containing terminal boutons with both clear and dense core vesicles in close approximation with the endocrine cells. 相似文献
26.
大鼠皮质神经元的体外培养和纯化 总被引:8,自引:2,他引:6
目的:探讨大鼠皮质神经元分离纯化的有效方法。方法:取胎鼠大脑皮质细胞,分别在不同的培养液中进行原代培养,利用相差显微镜对培养的细胞进行动态观察;并以免疫细胞化学技术对神经元进行染色。结果:在相差显微镜下可见加用N2添加剂和胎牛血清培养的神经元生长良好,胞体形态多样,突起数目多,长度长,与邻近神经元的胞体或突起形成接触,而胶质细胞则大量死亡,神经元纯化率达94%以上,未加用N2添加剂培养的神经元胶质细胞大量存在,神经元纯化率达50%左右。结论:N2添加剂和胎牛血清联合应用可使大鼠皮质神经元得到良好的生长和有效纯化。 相似文献
27.
T. L. Naneishvili M. G. Dashniani G. I. Machavariani 《Neuroscience and behavioral physiology》1987,17(1):49-55
Several types of neurons were differentiated on the basis of a study of neuronal activity in various parts of the cortex near the sulcus principalis during the execution of spatial delayed reactions by monkeys. It was established that the different types of neurons are represented in different numbers in. different parts of the cortex near the sulcus principalis. The determination of several factors influencing the activity of these neurons and the comparison of data on their quantitative representation in the anterior, middle, and posterior parts of the cortex near the sulcus principalis with the existing behavioral data obtained after local ablations of identical regions of the brain made it possible to postulate that neurons belonging to the different types are involved in the analysis of different processes and represent different functional units.Translated from Fiziologicheskii. Zhurnal SSSR imeni I. M. Sechenova, Vol. 71, No. 12, pp. 1488–1494, December, 1985. 相似文献
28.
Nonlinear sensory cortex response to simultaneous tactile stimuli in writer's cramp. 总被引:1,自引:0,他引:1
Terence D Sanger Alvaro Pascual-Leone Daniel Tarsy Gottfried Schlaug 《Movement disorders》2002,17(1):105-111
Writer's cramp is a task-specific dystonia that leads to involuntary hand postures during writing. Abnormalities of sensory processing may play a pathophysiological role in this disorder. Electrophysiology studies in a monkey model of focal dystonia have revealed de-differentiation of sensory maps and the existence of single cells in hand regions of area 3b with enlarged receptive fields that extend to the surfaces of more than one digit. These changes may lead to abnormal processing of simultaneous sensory inputs. To study abnormal processing of simultaneous sensory information in adult humans with writer's cramp, we used functional magnetic resonance imaging to compare the response in primary sensory cortex with simultaneous tactile stimulation of the index and middle finger, with the response to stimulation of each finger alone. We tested five patients with writer's cramp and seven unaffected (normal) subjects. In the normal subjects, a linear combination of the activation patterns for individual finger stimulation predicts the pattern of activity for combined stimulation with 12% error. In writer's cramp patients, the linear combination predicted the combined stimulation pattern with 30% error. Results indicate a nonlinear interaction between the sensory cortical response to individual finger stimulation in writer's cramp. This altered interaction may contribute to the motor abnormalities. 相似文献
29.
Zoï Kapoula Maria Pia Bucci Frederic Jurion Julie Ayoun Farzaneh Afkhami Dominique Brémond-Gignac 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》2006,245(7):931-936
Aim There is a controversy as to whether dyslexic children present visuo-motor disabilities such as vergence and accommodative
problems assessed with orthoptic tests. The purpose of this study is to re-examine this issue in a large population of children.
Methods Extensive orthoptic evaluation was made in 57 dyslexic and 46 non-dyslexic (“normal”) age-matched children. Convergence and
divergence capacities were evaluated at two distances (30 cm and 400 cm).
Results Binocular vision measured with stereo-acuity tests was normal in dyslexics. In contrast, the near point of convergence was
significantly more remote in dyslexics; most importantly, divergence at both far and near distance was significantly more
reduced in dyslexics (median value 4 pD and 10 pD, respectively, at far and near) than in “normals” (median value 6 pD and
12 pD, at far and near).
Conclusion The existence of the divergence deficit at far distance indicates the presence of deficit of divergence per se, independently
from convergence and accommodation relaxation. This result is novel and corroborated by physiological studies indicating distinct
control of convergence and divergence, both at the cortical and subcortical premotor level. We conclude that vergence deficits
are frequently present in dyslexics, and that dyslexics should be re-educated; training should address distinctively convergence
and divergence subsystems. 相似文献
30.
Pedophilia is linked to reduced activation in hypothalamus and lateral prefrontal cortex during visual erotic stimulation. 总被引:2,自引:0,他引:2
Martin Walter Joachim Witzel Christine Wiebking Udo Gubka Michael Rotte Kolja Schiltz Felix Bermpohl Claus Tempelmann Bernhard Bogerts Hans Jochen Heinze Georg Northoff 《Neuropsychopharmacology》2007,62(6):698-701
BACKGROUND: Although pedophilia is of high public concern, little is known about underlying neural mechanisms. Although pedophilic patients are sexually attracted to prepubescent children, they show no sexual interest toward adults. This study aimed to investigate the neural correlates of deficits of sexual and emotional arousal in pedophiles. METHODS: Thirteen pedophilic patients and 14 healthy control subjects were tested for differential neural activity during visual stimulation with emotional and erotic pictures with functional magnetic resonance imaging. RESULTS: Regions showing differential activations during the erotic condition comprised the hypothalamus, the periaqueductal gray, and dorsolateral prefrontal cortex, the latter correlating with a clinical measure. Alterations of emotional processing concerned the amygdala-hippocampus and dorsomedial prefrontal cortex. CONCLUSIONS: Hypothesized regions relevant for processing of erotic stimuli in healthy individuals showed reduced activations during visual erotic stimulation in pedophilic patients. This suggests an impaired recruitment of key structures that might contribute to an altered sexual interest of these patients toward adults. 相似文献