Cell block findings from residual PreservCyt samples in unsatisfactory ThinPrep Paps: no additional benefit

Our objective was to determine whether cell blocks (CB) performed on unsatisfactory ThinPrep Pap Test residual samples rendered additional clinically significant pathologic findings not detected in the original ThinPrep Pap smears. One hundred consecutive ThinPrep Paps categorized as unsatisfactory were selected for this study. The cytologic diagnosis of unsatisfactory was based on lack of cellularity (squamous) or complete obscuring blood or inflammation. Residual PreservCyt samples from these consecutive unsatisfactory ThinPrepPaps were used to prepare a cell block, using the inverted filter technique. One hematoxylin-eosin (H&E)-stained slide was prepared and evaluated. The amount and the cellular changes were noted for all smear constituents and compared with the original slides. The average patient age was 32 +/- 11 yr. The ThinPrepPap was performed as a follow-up on a previous abnormal smear (12%), pregnancy (25%), abnormal bleeding (22%), or previous unsatisfactory ThinPrepPap (5%), or as routine screening (36%). Variable amounts of endocervical cells, endometrial cells, and metaplastic cells, as well as a variable number of squamous epithelial cells, were present in 89%, 9%, 50%, and 35% in the CB method vs. ThinPrep, respectively. Abnormal cellular changes were noted in 15% of patients (9% with LSIL, and 6% with atypia of undetermined significance); 9 patients with squamous dysplasia had a previous abnormal ThinPrep Pap. A follow-up Pap smear and/or cervical biopsy were performed in 10 of the 15 patients with abnormal cytology detected on CB method revealing: LSIL (1 patient), HSIL (1 patient), ASCUS (1 patient), and within normal limits (7 patients). The findings indicate that the preparation of a CB does not contribute significantly. Importantly, no carcinomas or HSILs were identified on CB preparations. Since a large majority of lesions identified were LSILs, a repeat Pap smear in these cases represented a more cost-effective strategy than cell block preparation, although a cell block preparation may be useful in selected cases.     

The diagnostic reliability of urinary cytology: a retrospective study

The aim of this study was twofold. The first aim was to estimate the diagnostic reliability of urinary cytology for detection and management of urothelial neoplasms by using a specific preserving fluid for sample collection, and the liquid‐based thin layer method for specimen preparation, the estimate was based on the correlation between the cytological findings of 10,000 non‐hospitalized patients, and their histological diagnoses. A second aim was to compare the reliability of two instruments for thin‐layer preparation, i.e., TP2000, TP3000, capable of processing the specimens at very different rates. The preservation of cell structure is ameliorated by the procedure of sample collection and treatment here described. This allows a more accurate reading of LBC slides as shown by: (a) the significant concordance between cytological and histological diagnosis (92%); (b) the significant number of low‐grade urothelial carcinomas (20.5%) revealed by urinary cytology and validated by histologic diagnosis; (c) the low rate (8%) of misjudgement of cytological diagnosis reached in this study. The quality of performances of the two instruments tested for thin‐layer preparation, i.e., TP2000 and TP3000, is statistically comparable. We recommend the procedure that makes use of preserving fluid for sample collection (cytolyt?) and treatment (preservcyt ?) as here described. We also recommend the use of thin‐layer method for specimen preparation since it allows a more uniform distribution of the cells on the support with reduction of overlapping phenomena. Finally, economic considerations suggest the preferential use of Thin Prep 3000. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.     

液基细胞学在子宫颈病变筛查中的价值

目的:评价液基细胞学检查在宫颈病变筛查中的应用价值。方法:对经妇科检查初步诊断为宫颈炎的1800例患者行液基细胞学检查,其中阳性病例158例(ASCUS及以上病例),进一步行阴道镜下活检病理组织学检查,以病理学检查为金标准,细胞学结果与之相对照。结果:液基细胞学诊断为SCC、HSIL、LSIL的病例,病理符合率分别为100.00%、93.33%、60.00%。液基细胞学AGCUS34例中,有3例病理诊断为子宫内膜腺癌。结论:液基细胞学检查有助于宫颈癌、宫颈上皮内瘤变的检出;对细胞学为AGC的患者,有必要内膜分段诊刮,及时发现子宫内膜癌前病变及内膜癌。     

液基薄层细胞学检测在宫颈癌及癌前病变筛查中的应用

目的:分析液基薄层细胞学检测(thin-cytologic test,TCT)在宫颈癌筛查中的应用以及其与宫颈癌前病变及宫颈癌发生的相关性.方法:纳入2016年10月-2018年10月于我院妇科门诊进行宫颈筛查的患者2702例,所有患者均接受液基薄层细胞学检测、HPV-DNA检测及宫颈组织病理活检,比较2702例患者T...     

液基薄层细胞学检测对宫颈病变的诊断价值

目的探讨液基薄层细胞学检测（TCT）技术对宫颈癌前病变的诊断价值。方法收集宫颈病变患者1000倒,分别应用TCT（500例）检查及传统巴氏涂片（500例）检查。结果TCT法满意490例（98．0％）,高于传统涂片法的440例（88．0％）（χ^2=3．987,P〈0．05）;传统涂片法检查IIB～V级阳性50例（10．0％）低于TCT法的85例（17．1％）（χ^2=3．923,P〈0．05）;TCT检查与病理活检的阳性符合率64．7％,明显高于传统涂片法的44．0％（χ^2=3．893,P〈0．05）。结论TCT技术明显提高了标本的满意率及宫颈异常细胞检出率,并且与组织病理活检有较高的阳性符合率,适合于临床推广应用。     

液基薄层细胞检测诊断宫颈病变的临床研究

目的 探讨液基薄层细胞检测(TCT)在筛查和诊断宫颈疾病方面的价值.方法 对330例患者同时行巴氏涂片法与TCT法宫颈细胞学检测,对81例细胞学检测阳性(ASCUS/ASGUS及以上)者进一步行阴道镜下官颈活组织检查,观察比较其检测阳性率及准确度.结果 TCT法对异常细胞的阳性检出率明显高于巴氏涂片法(23.94%vs 16.06%,P<0.05),对重度细胞病变(LSIL及以上)的检出率提高42.92%.以阴道镜下宫颈活检为金标准,TCT法的阳性符合率显著高于巴氏涂片法(67.09%vs 45.28%,P<0.05 o结论与巴氏涂片法相比,应用TCT法筛查宫颈病变具有更高的敏感性TCT联合阴道镜下活检能提高宫颈癌前病变的诊断准确率.     

12672例子宫颈液基细胞学诊断结果分析

目的:利用液基薄层细胞技术(TCT)对12 672例宫颈标本进行细胞学检测,探讨宫颈癌的发病情况及TCT在妇科疾病诊断中的意义。方法:2006年2月～2008年2月进行宫颈细胞学检测的12 672例宫颈标本采用液基薄层细胞技术制片,根据《子宫颈细胞学Bethesda报告系统》(TBS)诊断系统做出诊断,并对结果进行回顾性分析。结果:12 672例宫颈标本中,发现鳞癌13例,占0.103%;腺癌3例,占0.024%;高度鳞状上皮内病变(HSIL)64例,占0.505%;低度鳞状上皮内病变(LSIL)146例,占1.152%,其中伴人乳头瘤病毒(HPV)感染90例,占0.710%;非典型鳞状细胞(ASC)651例,占5.137%;非典型腺细胞(AGUS)107例,占0.844%;未见上皮内病变细胞或恶性细胞(NILM)11 688例,占92.235%。结论:TCT和TBS系统能全面准确地反映宫颈病变的情况。早期发现癌前病变、早期治疗,阻止病变升级是防治宫颈癌的关键。     

Comparison of ThinPrep UroCyte and cytospin slide preparations for gastrointestinal specimens: evaluation and retrospective performance review

Gastrointestinal (GI) tract cytology has high specificity but poor sensitivity for detecting GI tract cancer. Newer methods of slide preparation may improve cytology performance and additionally permit molecular slide-based assays that could improve diagnostic accuracy. A split-sample validation study compared slides prepared using ThinPrep UroCyte filters or a cytocentrifuge method with respect to cellularity, stain quality, and interpretation. In this 15-slide split-sample study, UroCyte slide preparations were judged to be superior to cytocentrifuge preparations, and the method was implemented for GI cytology in December 2006. To assess diagnostic performance for GI cytology, we retrospectively reviewed outcomes for one year before and after implementation of UroCyte filter slide preparation. Sensitivity, specificity, positive predictive value, and negative predictive value for both slide preparations were largely equivalent to one another and compared favorably with values in the literature, but varied greatly depending on how atypical and suspicious-atypical cases were defined for calculations. For biopsied biliary samples, the highest sensitivities were observed when all atypical and suspicious-atypical cases were considered positive for malignancy, but were lower when suspicious-atypical cases were considered positive and atypical cases were considered negative for malignancy. This highlights the difficulty with comparing studies that define atypical classes differently, and points to the need for a well-defined approach to performance evaluation that relates directly to how diagnostic information is used clinically. We conclude that the UroCyte filter slide preparation is valid for evaluation of GI cytology specimens and may simplify adjunct molecular testing such as FISH. This is the first reported use of UroCyte filters for preparation of GI specimens.