灵龟八法针法治疗抑郁性神经症的临床研究

目的:观察灵龟八法针法对于抑郁性神经症的疗效。方法:60例病人,随机分为灵龟八法组31例,普通针刺组29例,灵龟八法组为在普通针刺基础上加用灵龟八法,每周治疗3次,共治疗3个月。结果:治疗1、2、3个月后两组汉密尔顿抑郁量表(HAMD)计分均较治疗前明显降低,P<0.01,灵龟八法组均分别明显低于普通针刺组,P<0.01;分别于1、2、3个月后做疗效比较,灵龟八法组均优于普通针刺组,P<0.05;治疗3个月后中医证候评定量表计分和抑郁自评量表(SDS)计分比较:灵龟八法组计分均明显低于普通针刺组,均P<0.01。结论:灵龟八法针法对于抑郁性神经症是一种有效的治疗手段,值得临床推广。     

李翠萍治疗多囊卵巢综合征的临床经验

李翠萍教授认为多囊卵巢综合征发病机理在于肾虚,应从肾论治,同时结合女性月经生理周期中阴阳变化规律进行治疗。临证以补肾调周法为主,中西医结合,取得了较好疗效,并附验案1例佐证。     

Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid chromatography-diode array detection

A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was used to simultaneously to analyze characteristics of six indicator compounds in the traditional Chinese medicine (TCM) formulation Wen-Qing-Yin (WQY). Separate optimization was performed using a Cosmosil C18 column gradient method with 0.1% formic acid in both mobile phases of aqueous and acetonitrile (ACN), at a flow rate, detection wavelength, and sample volume of 1.8 mL/min, 268 nm, and 10 μL, respectively. The linear regression of six active compounds berberine (BER), baicalin (BAI), ferulic acid (FER), geniposide (GEN), hydorxymethoxylfurfural (HMF), and paeoniflorin (PAE) was produced at the concentration range of 10–2000 μg/mL. The method validation revealed an acceptable precision (intra- and inter-day precision < 3.39% and 4.11%, respectively) and recovery (85.60–110.45% and 86.58–110.90%), a recovery range of 86.61–109.42%, and sensitivity (limit of detection [LOD] and limit of quantification [LOQ] values were in the range of 0.03–3.13, and 0.08–9.38 μg/mL, respectively) while the calibration curves were linear with a correlation coefficient (R²) ranging from 0.9966 to 0.9989. The qualitative and quantitative analyses were performed by direct comparison of the peaks of the WCY extract to retention times of reference standards. Additionally, principal component analysis (PCA) successfully discriminated four purchased commercial samples of all six indicator constituents, and the present results indicate their comprehensive potential usefulness for qualitative and quantitative analyses of the WQY decoction and its commercial products.     

A reporter gene assay for determining the biological activity of therapeutic antibodies targeting TIGIT

T cell immunoglobulin and ITIM domain (TIGIT) is a novel immune checkpoint that has been considered as a target in cancer immunotherapy. Current available bioassays for measuring the biological activity of therapeutic antibodies targeting TIGIT are restricted to mechanistic investigations because donor primary T cells are highly variable. Here, we designed a reporter gene assay comprising two cell lines, namely, CHO-CD112-CD3 scFv, which stably expresses CD112 (PVRL2, nectin-2) and a membrane-bound anti-CD3 single-chain fragment variable (scFv) as the target cell, and Jurkat-NFAT-TIGIT, which stably expresses TIGIT as well as the nuclear factor of activated T-cells (NFAT) response element-controlled luciferase gene, as the effector cell. The anti-CD3 scFv situated on the target cells activates Jurkat-NFAT-TIGIT cells through binding and crosslinking CD3 molecules of the effector cell, whereas interactions between CD112 and TIGIT prevent activation. The presence of anti-TIGIT mAbs disrupts their interaction, which in turn reverses the inactivation and luciferase expression. Optimization and validation studies have demonstrated that this assay is superior in terms of specificity, accuracy, linearity, and precision. In summary, this reliable and effective reporter gene assay may potentially be utilized in lot release control, stability assays, screening, and development of novel TIGIT-targeted therapeutic antibodies.     

Effect of Intra‐wound Vancomycin for Spinal Surgery: A Systematic Review and Meta‐analysis

Intra‐site prophylactic vancomycin in spine surgery is an effective method of decreasing the incidence of postsurgical wound infection. However, there are differences in the prophylactic programs used for various spinal surgeries. Thus, this systematic review and meta‐analysis aimed to evaluate the effectiveness of using intra‐wound vancomycin during spinal surgery and to explore the effects of dose‐dependence and the method of administration in a subgroup analysis. A total of 628 citations or studies were searched in PubMed, Ovid, Web of Science, and Google Scholar that were published before August 2016 with the terms “local vancomycin”, “intra‐wound vancomycin”, “intraoperative vancomycin”, “intra‐site vancomycin”, “topical vancomycin”, “spine surgery”, and “spinal surgery”. Finally, 19 retrospective cohort studies and one prospective case study were eligible for inclusion in the systematic review and meta‐analysis. The odds of developing postsurgical wound infection without prophylactic local vancomycin use were 2.83‐fold higher than the odds of experiencing wound infection with the use of intra‐wound vancomycin (95% confidence interval, 2.03–3.95; P = 0.083; I² = 32.2%). The subgroup analysis including the dosage and the method of administration, revealed different results compared to previous research. The value of I² in the 1‐g group was 27.2%, which was much lower than in the 2‐g group (I² = 57.6%). At the same time, the value of I² was 0.0% (P = 0.792, OR = 2.70) when vancomycin powder was directly sprinkled into all layers of the wound. However, there is high heterogenicity (I² = 60.0%, P = 0.007, OR = 2.83) when vancomycin powder is not exposed to the bone graft and instrumentation. There are differences found with the method of local application of vancomycin for reducing postoperative wounds and further studies are necessary, including investigations focusing on the dose‐dependent effects during spinal or the topical pharmacokinetic and other orthopaedic surgeries.     

原代培养大鼠肝细胞分离方法比较研究

目的:探索原代培养大鼠肝细胞的最佳分离方法。方法:对比观察不同的肝细胞分离技术的培养效果。培养前,以4％台盼蓝染色判定肝细胞活性,培养48h后,观察肝细胞贴壁及生长状况。结果:①灌流消化法优于剪切消化法;②在多种灌流消化法中,经门静脉灌流消化法优于经胆总管灌流消化法及经腹主动脉灌流消化法;③在门静脉灌流的基础上,0.1％胶原酶Ⅰ37℃热消化10～15分钟,效果最好,而0.25％胰蛋白酶消化10～12分钟次之,用含EDTA的D-Hank液直接灌流分离法效果极差。结论:原代培养肝细胞的得率及活性与不同分离法包括灌流途径、灌流液、消化时间等有关。     

尿中碘的过硫酸铵消化-砷铈催化分光光度测定方法

目的 介绍修订后的尿碘测定标准新方法，即尿中碘的过硫酸铵消化．砷铈(As^3 、Ce^4 )催化分光光度测定方法，建议各级实验室应尽快应用。方法 按“研制生物样品监测检验方法指南”(WS/T68-1996)的规定，对我国现行的尿碘测定方法标准进行了修订，并对修订后的新方法进行方法学评价。结果 修订后的新方法尿碘检测范围为0～300μg/L；最低检测量为3μg／L(取样量为0．25m1)；精密度：测定尿碘为63．3、129．8、252．5μg/L时，变异系数(CV)分别为5．5％、2．8％、4．4％(n=6)；准确度：对低、中、高碘3种尿样加碘标平均回收率分别为101．3％、97．6％、95．8％(n=6)，总平均回收率为98．3％(范围：92．6％～107．0％)；测定低、高2种尿碘标准物，其结果与给定值的相对偏差分别为4．9％和1．0％(n=6)；该方法的砷铈反应除了在水浴控温条件下进行，还可以直接在20～35℃之间某一稳定的室温条件下进行测定。结论 修订后的新方法比原标准方法更加简便易操作，并提高了测定的精密度和准确度。     

正交设计筛选抗幽门螺杆菌甲硝唑耐药株天然药物的最佳组合方案

目的筛选天然药物治疗幽门螺杆菌（Hp）甲硝唑耐药株的最佳组合方案。方法运用正交设计方法筛选臭灵丹、石榴皮、蒲公英三种天然药物抗坳甲硝唑耐药株的最佳组合。结果三种天然药物对Hp甲硝唑耐药株的最佳组合方案为：臭灵丹（MIC90，77．9mg／ml），石榴皮（MIC90，131．1mg／ml），蒲公英（MIC75，117．27mg／ml）。结论正交设计方法有效的筛选出了三种天然药物的最佳组合方案．提高了实验效率，节约科研资源。     

基于镅铍中子源的简易照射装置及其剂量学特性研究

目的 拟利用241Am-Be中子源设计并建立一套中子辐射照射实验装置.方法 采用蒙特卡洛(Monte Carlo)方法,模拟计算装置内外中子能谱和γ能谱空间分布数据,研究中子注量等随空间分布的变化规律;初步建立镅铍中子装置的数学模型,采用影锥法、平方反比律验证等数据分析方法,对中子输运过程进行研究.结果 模拟获得测量点...     

Stability-Indicating RP-HPLC Method for the Simultaneous Determination of Prazosin, Terazosin, and Doxazosin in Pharmaceutical Formulations

The current study was carried out with an attempt to separate similarly structured title drugs by liquid chromatography. Spectrophotometric techniques were generally insufficient under these conditions because of the spectral overlapping of drugs with similar functional groups. The pharmaceutical drugs prazosin, terazosin, and doxazosin contain the same parent quinazoline nucleus, thus making it especially difficult to separate the former two drugs because of their very similar structures. A simple and sensitive method for the routine determination of these drugs in pharmaceutical formulations was attempted. We found that the mobile phase consisting of A: ACN–diethylamine (0.05 ml), B: methanol, and C: 10 mM Ammonium acetate separated these drugs effectively. Separations were carried out on a new Kromasil C18 column (250 × 4.6 mm, 5.0 μm) at 254 nm wavelength. The calibration curve was found to be linear in the range of 2–500 μg/ml. The stated method was then validated in terms of specificity, linearity, precision, and accuracy. Additionally, the proposed method reduced the duration of the analysis.