引文——内容词分析法反映专题学科发展历史及现状的可靠性评价

以“妊娠糖尿病”专题为例,应用引文共引聚类方法揭示某一学科专题研究的发展历史,应用高频引文来源文献的内容词分析法揭示其研究现状。同时与专家的系统性综述相对比进行评价,结果得出引文—内容词分析法是反映专题发展历史及现状的可靠方法。     

家兔急性鼻窦炎模型两种建模方法的比较

目的探讨家兔两种急性鼻窦炎模型的制备方法，进行优良比较。方法健康成年新西兰白兔42只，随机分成窦口堵塞＋窦腔注入肺炎链球菌组（A组，18只）、鼻腔置入Merocel高分子膨胀海绵＋鼻腔注入肺炎链球菌组（B组，18只）及空白对照组（C组，6只）。A、B组动物分别于术后1、2、3周每组各处死6只；空白对照组于1周后处死。获取实验侧上颌窦、筛窦窦腔分泌物行细菌学检查，并将上颌窦、筛窦完整取出，全组织包埋行组织病理学观察。结果上颌窦分泌物细菌学培养结果示A、B两组家兔原发致病菌与机会致病菌感染差异无统计学意义（P〉0．05）；鼻窦黏膜组织病理学观察证实两种建模方法的成功率均为100％，B组造模方法可同时产生筛窦炎，且B组家兔上颌窦黏膜病理改变轻于A组，病理半定量化结果示两组间差异有统计学意义（P〈0．05）。结论鼻腔置入Merocel高分子膨胀海绵＋鼻腔注入肺炎链球菌的建模方法，具有人类鼻窦感染的病理生理特征，是一种更理想的急性鼻窦炎动物模型的制备方法。     

Simultaneous development of six LC-MS-MS methods for the determination of multiple analytes in human plasma

Traditional sequential single analyte method development is both time-consuming and labor-intensive. In this report, a concept of simultaneously developing multiple liquid chromatography coupled with tandem mass spectrometry (LC–MS–MS) methods were proposed. Mass spectrometric and chromatographic conditions as well as sample preparation methods for all analytes were optimized concurrently. Mass spectrometric conditions for six analytes, i.e. clonidine (CLO), albuterol (ALB), fentanyl (FEN), ritonavir (RIT), naltrexone (NAL), and loratadine (LOR), were established simultaneously using the Sciex Analyst software. LC–MS–MS sensitivities obtained using gradient elution methods on reversed-phase Inertsil ODS3 and normal phase Betasil silica columns were compared. Sample extraction methods using protein precipitation, liquid/liquid extraction, or solid-phase extraction (SPE) were evaluated. Recovery of analytes was determined. Matrix effects and interference due to endogenous compounds were investigated. Selection of a potential internal standard was discussed.     

A rapid method to visualize von willebrand factor multimers by using agarose gel electrophoresis, immunolocalization and luminographic detection

A highly sensitive and rapid clinical method for the visualization of the multimeric structure of von Willebrand Factor in plasma and platelets is described. The method utilizes submerged horizontal agarose gel electrophoresis, followed by transfer of the von Willebrand Factor onto a polyvinylidine fluoride membrane, and immunolocalization and luminographic visualization of the von Willebrand Factor multimeric pattern. This method distinguishes type 1 from types 2A and 2B von Willebrand disease, allowing timely evaluation and classification of von Willebrand Factor in patient plasma. It also allows visualization of the unusually high molecular weight multimers present in platelets. There are several major advantages to this method including rapid processing, simplicity of gel preparation, high sensitivity to low concentrations of von Willebrand Factor, and elimination of radioactivity.     

自我测量健康状况的新方法SRHMS运用

SRHMS作为测量健康状况的效用指标,能够从躯体、心理、社会适应三维的测量观念出发,综合性地自我评价健康状况。本文介绍和探讨了SRHMS的测量内容、测量方法以及在健康教育领域中的应用,为研究人群健康评价问题提供参考资料。     

试论《素问·热论》"可汗"、"可泄" --兼论"截断"、"扭转"

通过探讨《素问.热论》“可汗”、“可泄”两法以及后世发展的外感热性病各种治疗方法,对热病初期应用清热解毒来“截断”、“扭转”病势的方法提出异议,认为截断并扭转病势当遵循中医辨证论治精神,因时因地因人而施,既注意祛邪,更注意调护正气,务使邪有出路,从而达到改善症状,阻断病情发展,提高临床疗效,缩短病程的目的。     

ED-NM-MO三联法对丹参三七配比的多目标优化研究

[目的]采用ED-NM-MO三联法对经基线等比增减设计的丹参、三七不同配比的药效学数据进行非线性拟合和多目标优化。[方法]对经基线等比增减设计的丹参、三七不同配比的药效学数据,以心肌缺血程度Σ-ST、心肌缺血程度(缺血区左室)等7个分别反映心肌缺血、心脏状态和血流动力学的指标作为待优化的药效目标,进行非线性拟合和多目标优化。[结果]分别得到针对7个药效指标和6个药效指标(不包含血清中心肌钙蛋白)的Pareto最优配比。[结论]ED-NM-MO三联法是一种适合复方特点的优化方法,可以应用于由多饮片多组分多成分复方药物的剂量配比优化。     

透刺治疗周围性面神经麻痹

目的寻找和探讨治疗周围性面神经麻痹更为有效的透刺方法。方法按首诊先后顺序随机分成两组,治疗组50例,以阳白透头维,阳白(原穴略上)透上星、丝竹空透鱼腰、牵正透迎香、地仓透颊车、迎香与颊车互透,使透刺方向与局部瘫痪肌束约呈45°角。对照组45例,选穴基本同治疗组,常规透刺,以阳白透鱼腰,攒竹透鱼腰,地仓与颊车互透,牵正透地仓,四白透迎香。结果治疗组痊愈率76.0%,愈显率92.0%;对照组痊愈率55.6%,愈显率75.6%。两组痊愈率、愈显率及痊愈者所需疗次间差异有显著性意义。结论透刺方向与瘫痪肌束呈45°角的刺法更利于面瘫的恢复,疗效更高。     

督脉导气法治疗抑郁症睡眠障碍临床观察

目的：观察用督脉导气针法合并抗抑郁药物治疗抑郁症睡眠障碍的临床疗效，寻找治疗抑郁症睡眠障碍的有效方法。方法：将45例抑郁症睡眠障碍患者随机分为两组，治疗组23例用督脉导气针法合并抗抑郁药物治疗，取神庭、百会、大椎、神道、至阳为主穴，同时内服抗抑郁药物；对照组22例采用单纯抗抑郁药物治疗。疗程均为4周。观察两组汉密尔顿（HAMD）量表及匹兹堡睡眠质量指数（PSQI）量表治疗前后的变化情况。结果：HAMD量表分值在两组治疗前后差异均有非常显著性意义（P〈0．01），但PSQI量表分值仅在治疗组治疗前后差异有非常显著性意义（P〈0．01），而对照组治疗前后差异无显著性意义（P〉0．05）;治疗后治疗组与对照组相比，HAMD和PSQI量表分值差异均有非常显著性意义（P〈0．01）。结论：督脉导气针法合并抗抑郁药物治疗可显著改善抑郁症睡眠障碍。     

MYL-I蒙医疗术温针仪治疗膝关节骨性关节炎的临床观察

目的:观察MYL-I蒙医疗术温针仪对膝关节骨性关节炎的临床疗效。方法:运用蒙医MYL-I蒙医疗术温针仪替代传统蒙医温针治疗膝关节骨性关节炎,并与蒙医银针、蒙医传统温针进行对比。结果:MYL-I蒙医疗术温针仪组与蒙医传统温针组的疗效均优于蒙医银针组。MYL-I蒙医疗术温针仪组与蒙医传统温针组比较疗效无显著性差异。结论:MYL-I蒙医疗术温针仪可以完全替代蒙医传统温针疗法,且安全性优于蒙医传统温针疗法,同时具有操作简单、温度可调、无烟、副作用少等特点,值得推广应用。