冠通Ⅰ号对实验性兔动脉粥样硬化的影响

目的　观察冠通Ⅰ号对实验性兔动脉粥样硬化的作用。方法　新西兰大白兔 30只 ,随机分为对照组 (普通颗粒饲料 )、高胆固醇组 (普通颗料饲料 1.5 %胆固醇 )、药物干预组 (普通颗粒饲料 1.5 %胆固醇 冠通Ⅰ号 1.0g/kg·d)。实验前及实验后 9周 ,测定各组兔血清脂质、前列腺素I2 、血栓素B2 及观察主动脉病理学改变。结果　实验前各组兔血清总胆固醇 (TC)、甘油三脂 (TG)、低密度脂蛋白胆固醇 (LDL C)、血浆血栓素BC(TXB2 )、6 酮 前列环素F1α(6 keto PGF1α)均无统计学差异 (P >0 .0 5 )。实验 9周后 ,高胆固醇组、药物干预组血清TC、TG、LDL C及血浆TXB2 、TXB2 / 6 keto PGF1α比值均高于对照组 (P <0 .0 5 ) ,但药物干预组低于高胆固醇组 (P <0 .0 5 )。病理切片发现 :药物干预组兔主动脉粥样硬化程度较高胆固醇组兔主动脉粥样硬化程度轻。结论　冠通Ⅰ号具有调整脂质代谢、抗血小板、减轻实验性兔动脉粥样硬化的作用     

Combination Adenovirus-Mediated HSV-tk/GCV and Antisense IGF-1 Gene Therapy for Rat Glioma

Ａｄｅｎｏｖｉｒｕｓ ｍｅｄｉａｔｅｄＨＳＶ ｔｋ/ＧＣＶｓｙｓｔｅｍｈａｓｂｅｅｎｅｘｔｅｎｓｉｖｅｌｙａｐｐｌｉｅｄｉｎｔｈｅｅｘｐｅｒｉｍｅｎｔａｌｉｎｖｅｓｔｉｇａｔｉｏｎｏｆｇｌｉｏｍａａｎｄｉｔａｌｓｏｈａｓｂｅｅｎｐｕｔｉｎｔｏｔｈｅｃｌｉｎｉｃａｌｐｈａｓｅＩｔｒｅａｔｍｅｎｔ .ＳｅｖｅｒａｌｓｔｕｄｉｅｓｒｅｐｏｒｔｅｄＨＳＶ ｔｋ/ＧＣＶｓｙｓｔｅｍｍａｙｍａｋｅｔｈｅｂｒａｉｎｔｕｍｏｒｓｒｅｇｒｅｓｓｅｄｔｏｔａｌｌｙａｎｄｔｈｅｓｕｒｖｉｖａｌｐｅｒｉｏｄｏｆｔｈｅｔｕｍｏ…     

Effect of a few histamine1-antagonists on blood glucose in patients of allergic rhinitis

Histamine₁, receptor antagonists are being prescribed frequently in ENT practice. However, previously we found a fall of blood glucose with terfenadine and astemizole. Present study (double blind, randomized, single dummy and placebo controlled) evaluated the chronic effect (7 days of use) of clemastine, Loratidine and Certrizine on blood glucose level in patient of allergic rhinitis. Cetrizine produced a significant increase in post-prandial blood glucose (P<0.02) and a little rise in fasting blood glucose. Clamastine caused a little fall of fasting and a little rite of post-prandial blood glucose levels. The exact mechanisms involved in the effect of these drugs on blood glucose remain to be elucidated.     

A Dutch family with progressive sensorineural hearing impairment linked to the DFNA2 region

We studied a Dutch family with DFNA2-linked progressive sensorineural hearing impairment (SNHI). Recent audiograms were obtained from 18 of the affected persons (age 7–81 years) and were used in a gene-linkage analysis. Linear regression analysis of the audiograms, using binaural mean thresholds, disclosed on average a descending slope of approximately 10 dB/octave at any age and an annual threshold increase at any frequency of about 0.7 dB/year. There may have been substantial congenital impairment at higher frequencies, but longitudinal analysis of hearing impairment in the youngest case, who was followed from age 5 years, suggested that the most significant changes in hearing may have occurred in the first two decades of life. Linkage analysis was carried out with special attention to the DFNA2 region because hearing trends were very similar to families previously linked to DFNA2. Linkage to DFNA2 was established with maximum lod scores of 4.7 and 3.2 for the flanking markers of the DFNA2 region (D1S432;MYCL1). Received: 25 February 1999 / Accepted: 11 June 1999     

Quantitative Gef??analyse im marginalen Parodont mit Hilfe von PECAM-1

BACKGROUND: Systemic sclerosis (SSc) is a generalized disorder of the interstitial tissues and vasculature with distinct abnormalities in three systems, immune and autoimmune, vascular and microvascular, and mesenchymal extracellular matrix (ECM), that lead to exuberant fibrosis. The aim of this study was to compare the number of blood vessel profiles in the marginal gingiva between SSc patients and patients with periodontitis but without SSc by using biopsies. METHODS: Marginal gingiva and gingival papilla were obtained from 13 scleroderma patients and 8 patients with periodontitis after routine tooth extraction and gingival curettage. On the histological sections, immunohistochemical investigations were performed using the avidin-biotin complex method (ABC) and the monoclonal antibody CD 31/Clone JC70A (platelet endothelial cell adhesion molecule-1). Blood vessels were identified by light microscopy (original magnification x400) and counted within 0.3615 mm2. Medians of blood vessel profiles were compared by Mann-Whitney U-test. RESULTS: There is no statistical difference between the median of blood vessel profiles in the marginal gingiva of SSc patients as compared to the median of blood vessel profiles in the marginal gingiva of patients with periodontitis (P = 0.665). We did not discover avascular areas in the subepithelial connective tissue. DISCUSSION: The pathological changes in the microvasculature in the dermis of SSc patients are not transferable to the marginal periodont, as intraindividual histological examinations of dermis and oral mucosa in relation to the degree of the disease are not available yet.     

高健美延缓衰老的临床研究

目的 观察增龄对人血清中甲状腺激素（ＴＨ）、胰岛素样生长因子－１（ＩＧＦ－１）及脱氢表雄酮（ＤＨＥＡ）变化及用高健美治疗老年人后甲状腺功能（甲功）、ＩＧＦ－１、ＤＨＥＡ和肾虚衰老症状的改变。方法 从健康体检的人群中,选择５０例属于肾虚衰老的老年人作为治疗组,选择老年前期及青壮年期各３０例作为对照组,分别检测甲功（ＴＴ３、ＴＴ４、ＦＴ３、ＦＴ４）,ＩＧＦ－１、ＤＨＥＡ、肝功能及心电图;对治疗组用小剂     

The mutation of insulin receptor substrate-1 gene in Chines e patients with non-insulin-dependent diabetes mellitus

Objective To identify the relationship between mutation in the insulin receptor substrate-1 (IRS-1) gene and the incidence of non-insulin-dependent diabetes mellitus (NIDDM) in the Chinese population. Methods Samples were obtained from 68 Chinese patients with NIDDM and 68 control subjects. The +1700～+4437 bp fragment of the IRS-1 gene was screened by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. All SSCP variations were submitted to DNA sequence analysis. Results Two amino acid variations ［GGG →AGG(G(971) R) and CCT→TCT(P(1079) S)］ and 3 silent mutations ［GAT→GAC(D(422) D), CCA→CCC(P(737) P) and GCA→GCG(A(804) A)］ were identified, among which the CCA→CCC(P(737) P) and CCT→TCT(P(1079) S) have not been previously reported. All five variations were found in Chinese patients with NIDDM, while GCA→GCG(A(804) A) was the only one found in control subjects. The overall incidence of the five variations in Chinese patients with NIDDM were much higher than that in control subjects (38.2% vs 7.4%, χ(2)=18.42,P&lt;0.01). The most common polymorphism in the Chinese population was GCA→GCG(A(804) A), and its frequency was significantly higher in Chinese patients with NIDDM than in controls (26.5% vs 7.4%, χ(2)=8.84,P&lt;0.01). The homozygotes of the variation in patients with NIDDM and control subjects were 8.8% and 1.5%, respectively (χ(2)=2.41,P&gt;0.05). Conclusion These results indicate that there may be a relation between these nucleotide variations of IRS-1 gene and Chinese patients with NIDDM     

Relationship between serum or tissue ouabain and blood pressure in 1k1c hypert ensive rats

Objective To evaluate the role of endogenous ouabain (EO) in the development of hypertension and the characteristics of EO secretion in 1k1c (one kidney, one clipped) hypertensive rats. Methods EO content of serum and tissues in 1k1c hypertensive rats and normal control Sprague-Dawley (SD) rats was detected by the method of enzyme linked immunosorbent assay (ELISA). The relationship between serum or tissue ouabain and blood pressure was analyzed in 1k1c hypertensive rats. Results The ouabain content of serum, heart, kidney, adrenal gland, pituitary and hypothalamus was significantly higher in 1k1c hypertensive rats than that in normal control SD rats (2.25, 2.63, 3.35, 40.37, 3.34, 15.7 μg/kg tissue in 1k1c hypertensive rats vs 1.12, 1.79, 1.73, 27.54, 1.83, 10.10 μg/kg tissue in control SD rats, respectively. P&lt;0.05 for all of these comparisons). The ouabain content of the adrenal gland and the hypothalamus was higher than that of other tissues or serum, both in 1k1c rats and in control SD rats. The EO content of serum, kidney and hypothalamus was significantly correlated with blood pressure in 1k1c hypertensive rats (r=0.59, 0.63, 0.52, respectively. P&lt;0.05). The ouabain content of heart, liver, adrenal gland and pituitary was not correlated with blood pressure. Conclusions EO might play an important role in the development of hypertension in 1k1c hypertensive rats. The adrenal gland may be a major source of EO and the hypothalamus-pituitary-adrenal axis may be involved in the regulation of EO secretion.     

The role of c-myc in regulating mdr1 gene expression in tumor cell line KB

Ｔｈｅｃ ｍｙｃｐｒｏｔｏ ｏｎｃｏｇｅｎｅｐｌａｙｓａｒｏｌｅｉｎｍａｎｙｃｅｌｌｕｌａｒｐｒｏｃｅｓｓｅｓ ,ｓｕｃｈａｓｐｒｏｌｉｆｅｒａｔｉｏｎ ,ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎａｎｄａｐｏｐｔｏｓｉｓ Ｉｔｉｓａｎｅａｒｌｙ ｒｅｓｐｏｎｓｅｇｅｎｅｎｅｃｅｓｓａｒｙｆｏｒｃｅｌｌ ｃｙｃｌｅｐｒｏｇｒｅｓｓｉｏｎ (Ｇ1 Ｓｔｒａｎｓｉｔｉｏｎ)ａｎｄａｃｔｉｖａｔｅｓｑｕｉｅｓｃｅｎｔｃｅｌｌｓｉｎｔｏｔｈｅｃｅｌｌｃｙｃｌｅ (Ｇ0 Ｇ1ｔｒａｎｓｉｔｉｏｎ) Ｄｏｗｎ ｒｅｇｕｌａｔｉｏｎｏｆｃ …     

γ射线诱导人肝癌细胞表达B7-1共刺激分子的研究

目的　研究电离辐射与肿瘤细胞B7- 1分子表达之间的关系 ,探讨肿瘤细胞辐照后免疫原性增强的机制。方法　采用间接免疫荧光 -流式细胞仪分析技术 ,研究在用不同剂量γ射线照射SMMC - 772肝癌细胞后、培养不同时间内SMMC - 772细胞B7- 1共刺激分子的表达水平。并用3H -TdR释放法测定照射和未照射肿瘤细胞与淋巴细胞共反应后的细胞毒活性。结果　未照射和经 10、2 0、30Gy照射的人肝癌细胞不表达B7- 1分子。经 40、5 0、6 0Gy剂量照射后 ,SMMC - 772肝癌细胞表达B7- 1分子 ,最高达 6 6 .8± 1.3% ,与对照组比有非常显著性差异 (P <0 .0 1)。表达时间的高峰在照射后培养 72h时 ,最低在照射后培养 2 4h时 (P <0 .0 5 )。细胞毒活性测定结果表明 ,表达B7- 1分子的SMMC - 72肝癌细胞与淋巴细胞共反应后 ,细胞毒活性明显高于未照射组 (P <0 .0 1)。结论　γ射线可诱导肝癌细胞表达B7- 1分子 ,从而增强肿瘤细胞的免疫性