Identification of a new HLA-B*15 allele,HLA-B*1569

We have identified a new HLA-B*15 allele (B*1569) by polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing (SBT). This novel allele was found in a 67-year-old white Caucasian male and differs from HLA-B*1503 at 3 positions. The nucleotide substitutions at positions 544, 559 and 560 result in amino acid changes at codon 158 from GCC (alanine) to ACC (threonine), and at codon 163 from CTG (leucine) to ACG (threonine).     

A组轮状病毒广州地方株VP7基因序列的比较分析

目的 了解我国轮状病毒G1型广州地方株与标准株及北京G1型地方株VP7基因序列的差异，为我国轮状病毒疫苗的研制提供资料。方法 通过逆转录—聚合酶链反应(RT—PCR)获得了轮状病毒广州地方株R97—196 VP7全基因的cDNA片段，将其克隆入T—A克隆质粒pUCm—T中，构建成重组质粒pUCmT—VP7，对克隆的VP7基因进行序列测定。结果 该地方株的基因核苷酸全长为1062nt，读码框架和以往的研究一致，和北京G1型地方株173的VP7氨基酸序列具有高度同源性(98％)，而与不同血清型标准株间则变异较大(73％—81％)，氨基酸序列中存在的一些高变区和保守功能区与已报道的研究结果一致。从进化角度分析，与轮状病毒标准株Wa株，相距较远。结论 轮状病毒广州地方株R97—196 VP7基因片段属G1型，轮状病毒VP7基因的变异与地域有一定关系。     

中国庚型肝炎病毒河南株非结构基因（NS）3区cDNA的克隆 …

目的 为了研究中国庚型肝炎病毒（ＨＧＶ）非结构（ＮＳ３）区基因结构特征。方法 利用逆转录－半巢式－聚合酶链反应从河南１份ＨＧＶ ＲＮＡ阳性血清获得覆盖ＨＢＶ ＮＳ３全长ｃＤＮＡ的４个片段，并克隆到ｐｃＤＮＡⅡ载体中，采用Ｓａｎｇｅｒ双脱氧末端终止法测定全部ｃＤＮＡ序列。结果 发现克隆到的包括ＨＢＶ ＮＳ３区在内的ｃＤＮＡ序列和度为２１３７个核苷酸，编码７１１个氨基酸。与国内外已测定的５株全序列的相     

汉坦病毒A9株全长L片段cDNA克隆和核苷酸序列测定

目的克隆我国分离的汉坦病毒A9株L片段全长cDNA,并测定其核苷酸序列.方法用逆转录-聚合酶链反应(RT-PCR)技术分段扩增汉坦病毒A9株全部L片段,用T-A克隆方法进行PCR产物克隆,测定PCR产物的核苷酸序列.通过亚克隆将分段的L片段连接成全长cDNA克隆.结果A9株的基因组L片段长度为6533个核苷酸,腺嘌呤核苷酸和尿嘧啶核苷酸丰富(%A+U=62.47).包含有一个单一的开放读码框架(ORF),编码一个标准的质量为2.46×105的蛋白,含有2151个氨基酸.A9株与76-118、C1-1和C1-2株的同源性最高,达到83.8%.与TULA病毒的关系较远,其核酸序列的同源性为65.8%.将推导的A9株编码的氨基酸序列与其他21种负链RNA病毒的依赖RNA的RNA聚合酶的氨基酸序列以及汉坦病毒几个代表株的L片段氨基酸序列进行比较,显示A9编码的RNA聚合酶也有6个比较保守的区域以及几个极端保守的氨基酸残基.结论汉坦病毒A9株L片段具有和其他汉坦病毒RNA聚合酶相似的核苷酸一级结构,通过对推导的氨基酸分析,该片段具有一些在RNA病毒聚合酶中都存在的保守区域.     

Pseudorandom testing technique for the characterization of local hemodynamic control

Summary In order to investigate the low frequency properties of renal and femoral hemodynamic variables, pseudorandom testing techniques were used. The arterial flow of each bed, in separate experiments, was modulated by a low amplitude signal based on a pseudorandom binary sequence (PRBS) generated by digital computer.The cross-correlation functions between input flow and arterial pressure, venous pressure, and venous flow exhibit damped oscillations in all cases. These responses are parameterized in terms of a damping ratio () and an undamped natural frequency _n for a second order model. The parameters of the model are dependent upon the state of the bed as defined by mean arterial and venous pressures, mean flow through the bed, resistance, and oxygen consumption.The results of this study offer further insight into the dynamic low frequency autoregulation phenomenon for the renal and femoral beds of the dog.Supported by NIH Grant HE 11747.     

用RACE技术对一株肠道病毒3''端进行扩增和序列分析

目的 应用3′RACE技术对一株本室分离肠道病毒基因组3′末端进行扩增，并对其核苷酸序列进行比较分析。方法 提取病毒总RNA，以锚定oligdT（17）引物进行逆转录，用特异引物及锚定引物进行3′RACE扩增，将PCR产物进行克隆、测序和序列分析。结果 获得了该病毒的3′端核苷酸序列，同源性分析结果显示该肠道病毒的核苷酸序列与肠道病毒76、89、90、91型的同源性最高为90％左右，而与其他型别的肠道病毒的同源性均小于80％；推导的氨基酸同源性与肠道病毒76、89、90、91型均在90％以上。结论 用RACE技术对肠道病毒3′端进行扩增及序列分析，证明该病毒属新型肠道病毒类，为进一步研究该病毒的分子生物学特性奠定了基础。     

Assessment of Baroreceptor Reflex Function During Mental Stress and Relaxation

This paper describes a method of measuring baroreceptor cardiac reflex sensitivity noninvasively from spontaneous patterns of blood pressure and interbeat interval, and the application of this technique in psychophysiology. Baroreflex function was assessed in 24 female volunteers during relaxation and performance of the cold pressor test and a non-verbal mental arithmetic task. Blood pressure and interbeat interval were monitored continuously from the finger using the vascular unloading technique. Sequences of three or more cardiac cycles were identified over which systolic blood pressure increased progressively in conjunction with lengthening interbeat interval, or systolic blood pressure decreased as interbeat interval was reduced. The regression between systolic blood pressure and interbeat interval was computed as an index of baroreflex sensitivity. Relaxation was associated with a small prolongation of interbeat interval, whereas baroreflex sensitivity increased from 17.1 to 19.8 ms/mmHg. Baroreflex sensitivity was reduced significantly during mental arithmetic (mean 14.2 ms/mmHg) but not during the cold pressor test (mean 17.4 ms/mmHg). The difference between mental arithmetic and the cold pressor test may be related to the relative intensity of cardiac and vascular responses in the two situations. The implications of these results for the understanding of behavioural influences on haemodynamic function are discussed and the advantages of noninvasive methods are considered.     

贵州与中国大陆其它地区空肠弯曲菌MLST特征的比较分析

目的 了解贵州省鸡源空肠弯曲菌的分子流行特征,阐明各地菌株间的亲缘关系和进化特征。方法 在贵州省随机抽取7个大型养鸡场,共采集210份鸡的新鲜粪便样本,分离培养出38株空肠弯曲菌,采用多位点序列分型对分离株的序列型及克隆复合体进行构成和聚类分析,与PubMLST数据库中来自国内的分离株分子特征进行比较。结果 贵州省38株鸡源空肠弯曲菌分为23种ST型和8个克隆复合体,其中9种是目前中国大陆地区已有的ST型,2个等位基因和8种序列型为新发现的。ST型构成比前四位分别是ST - 45、ST - 161、ST - 354、ST - 4 324,与数据库中已有的6株贵州来源菌株相比,不存在共同的ST型。优势克隆复合体（CC - 45、CC - 354、CC - 52）与数据库中鸡源分离株的前三位克隆复合体没有重叠。菌株遗传进化树显示,本研究中属于优势克隆复合体的18株菌与国内部分人源、猪源、野生鸟源、鹅源分离株有较近的遗传距离。结论 贵州省鸡源空肠弯曲菌具有高度遗传多样性,在一定程度上表现出地域流行特征。序列型与国内华东地区的分离株有较高的交叉性,部分分离株与国内人、野生鸟、鹅等来源分离株遗传距离近,应加强对不同地区、不同宿主来源空肠弯曲菌的动态监测。     

献血员、肝炎患者TTV DNA检测及部分TTV基因序列分析

目的了解献血员、肝炎患者中的输血传递病毒(TTV)感染状况及基因型别。方法设计合成引物采用半套式聚合酶链反应（hemi-nestPCR）方法,对195份献血员血清、72份不同型别的肝炎患者血清进行了TTVDNA的PCR检测,并对部分阳性株进行序列测定及计算机分析。结果①在血清丙氨酸转氨酶（ALT）异常,HBsAg及抗HCV阴性的99份献血员血清标本中,检出TTVDNA阳性标本36份,阳性检出率为36.3%;而在ALT正常的96份献血员血清标本中,检出TTVDNA阳性标本16份,阳性检出率为16.6%,明显低于ALT异常献血者。②在72例不同肝炎患者中,共检出39例TTVDNA阳性血清,总检出率为54.16%,在非甲-非庚型肝炎患者中TTVDNA阳性率为87.5%,而在明确诊断为甲-庚型肝炎的患者中TTVDNA阳性率为50%,两组相比差异显著（P<0.05）。对8株阳性株序列分析结果显示,测序的8个分离株与9个G1、G2代表株核酸的同源性为60.4%～99.1%,氨基酸的同源性为55.4%～98.6%。经系统发育分析表明,这8个TTV分离株中有7株可分属于G1、G2两个基因型的5个亚型,而另1株为G1型的新亚型。结论①献血者中存在TTV感染,提示TTV可以导致感染个体的肝功能异常,TTV可能与HBV感染相似,亦存在“健康携带状态”。②非甲-非庚型肝炎患者的TTV感染率明显高于明确病原的甲-庚型肝炎患者,TTV感染与肝炎有关,可能是非甲-非庚型肝炎的病原之一。③8个TTV分离株中7个分属于G1、G2两个基因型中的5个亚型,另有1株为G1型的新亚型。     

Design and application research of nucleotide sequence analysis system software

INTRODUCTION　　From integrallevel and celllevel,the research of organism and diseasediagnosishave already entered molecular level.Molecular biology is the science to researchstructure and function of organism macromolecule.Based on differentgene code se-…