利培酮治疗与细胞色素P4502D6/C188T酶基因多态性的关联分析

目的　研究利培酮临床效应的个体差异与其代谢酶细胞色素P4 5 0 2D6 (cytochromeP4 5 02D6 ,CYP2D6 )酶基因多态性的相关性。方法　对 88例符合CCMD 3精神分裂症诊断标准的患者和 96例健康对照者作病例 -对照分析。精神分裂症患者给予利培酮治疗 8周 ,用阳性和阴性症状量表 (posi tiveandnegativesymptomscale ,PANSS)评分评价利培酮疗效。采用聚合酶链反应扩增及限制性片段长度多态性 (PCR RFLP)技术对CYP2D6exonⅠ的C188T位点突变进行检测 ,分析利培酮临床效应与其主要代谢酶CYP2D6 /C188T酶基因多态性的相关性。结果　中国上海地区人群的CYP2D6 /C188T突变率(弱代谢型 )为 36 .3% ,病例组和正常对照组间基因型频率总体分布比较无显著差异 (χ2 =1.15 ,df=2 ,P >0 .0 5 ) ,两组间的等位基因频率之间比较也无显著性差异 (χ2 =0 .78,df=1,P >0 .0 5 )。进行性别及有否家族史分组后分析 ,亦无差异存在 ,且CYP2D6 /C188T突变与利培酮临床效应之间并无相关性 (χ2 =1.12 ,df=2 ;χ2 =0 .0 3,df=1,P >0 .0 5 )。结论　未发现中国人CYP2D6 /C188T多态性与利培酮临床效应的个体差异有相关性。     

病毒性心肌炎患者血清IL-6的检测及其临床意义

为了观察病毒性心肌炎患者血清 IL-6的水平变化 ,我们对 1 8例病毒性心肌炎患者血清 IL -6水平进行了检测 ,并与正常对照组进行比较 ,现报告如下。对象与方法1　对　象　健康对照组 48例 ,男 2 5例 ,女2 3例 ,年龄 2 5～ 58岁 ,平均年龄 44 .0 5± 1 1 .76岁。病毒性心肌炎组 1 8例 ,男 7例 ,女 1 1例 ,年龄1 8～ 45岁 ,平均年龄 3 9.4± 8.56岁。按 1 995年全国心肌炎、心肌病专题会议诊断参考标准入选 ,排除急、慢性肝病 ,其中急性心肌炎 1 5例 ,慢性心肌炎 3例。2　方　法　正常对照组、心肌炎组均空腹抽静脉血 3 ml,分离血清后置于 -2…     

白花前胡甲素对心肌缺血再灌注大鼠血清IL-6水平的影响

目的　探讨白花前胡甲素 (Pd Ia)抗缺血再灌注损伤的机制 ,为寻找高效、低毒及安全的防治缺血再灌注损伤的药物提供理论基础。方法　采用放射免疫分析测定血清IL— 6水平 ,并通过常规HE染色比较中性粒细胞等炎性细胞的心肌浸润情况。结果　Pd Ia低、中、高三个剂量组IL— 6水平分别由对照组的 (15 2 88±15 2 5 ) pg/ml降至 (12 8 45± 18 37)pg/ml、 (119 6 6± 15 72 ) pg/ml和 (116 13± 10 45 )pg/ml (P <0 0 1) ,其作用强度与硝苯地平相近 (12 3 5 5± 18 6 2 )pg/ml。 结论　Pd Ia明显抑制缺血再灌注大鼠血清IL— 6水平 ,提示Pd Ia可能通过抑制NF—kB途径实现再灌注性损伤的防治     

Interactions Between Cytomegalovirus, Human Herpesvirus-6, and the Recurrence of Hepatitis C After Liver Transplantation

Recurrence of hepatitis C (HCV) following liver transplantation is common. Herpesvirus reactivation following transplant may have an immunomodulatory effect resulting in increased HCV replication. We studied whether cytomegalovirus (CMV) and human herpesvirus-6 (HHV-6) may be associated with HCV recurrence and viral load after transplant. We prospectively followed 66 HCV liver-transplant recipients with serial viral load testing for CMV and HHV-6. Infection and viral load were correlated with the development of biopsy-proven HCV recurrence and HCV viral loads. Histologic recurrence of HCV occurred in 41/66 (62.1%) patients. In the primary analysis, CMV infection and disease, and HHV-6 infection were not associated with HCV recurrence. Peak CMV and HHV-6 viral loads were not significantly different in patients with and without recurrence. No correlation was observed between HCV viral loads at 1 and 3 months post-transplant and peak HHV-6 or CMV viral loads. In a subgroup analysis, HHV-6 infection was associated with the development of more severe recurrence (hepatitis and/or fibrosis score > or = 2) (p = 0.01). Also, fibrosis scores at last follow up were higher in patients with CMV disease (1.67 vs. 0.56; p = 0.016) and in patients with HHV-6 infection (1.18 vs. 0.55; p = 0.031). In conclusion, HHV-6 and CMV infection and viral load were not associated with increased overall rates of HCV recurrence or HCV viral load after liver transplantation but may be associated with more severe forms of recurrence.     

脑胶质瘤白细胞介素-6自分泌或旁分泌环路的研究

目的　研究白细胞介素 6(IL 6) /IL 6受体 (IL 6R)自分泌或旁分泌环路与脑胶质瘤的关系。方法　采用逆转录 聚合酶链反应 (RT PCR)方法检测IL 6和IL 6R基因在 4株脑胶质瘤细胞株、62例脑胶质瘤组织标本、5例良性脑膜瘤组织和 5例人正常脑胶质细胞中的表达 ;将IL 6单抗 (McAb)或IL 6RMcAb与脑胶质瘤细胞共同孵育 ,四甲基偶氮唑蓝 (MTT)比色法检测对细胞生长的抑制作用。结果　脑胶质瘤细胞株C6、9L、U 2 5 1和SHG44均有IL 6和IL 6R的表达 ,脑胶质瘤组织标本中 47例 (75 .81% )表达IL 6、5 2例 (83 .87% )表达IL 6R、41例 (66.13 % )同时表达IL 6和IL 6R ;良性脑膜瘤组织 4例表达IL 6,人脑正常胶质细胞仅有IL 6弱表达 ,两者均无IL 6R表达。IL 6McAb和IL 6RMcAb能够显著抑制脑胶质瘤细胞的增殖 ,并存在剂量依赖关系(P <0 .0 1)。结论　脑胶质瘤很可能存在IL 6/IL 6R自分泌或旁分泌环路 ,阻断此环路可以明显抑制胶质瘤细胞的恶性增殖     

骨感染病人TNF-α、IL-6和C反应蛋白的检测及临床意义

①目的　探讨骨感染病人肿瘤坏死因子α(TNF α)、白细胞介素 6 (IL 6 )和C反应蛋白 (CRP)水平的变化及意义。②方法　采用双抗体夹心ELISA法测定 30例骨感染病人及 2 5例正常人的血清TNF α、IL 6水平 ,采用散射比浊法测定CRP水平。③结果　骨感染病人血清中TNF α、IL 6及CRP含量均处于高水平状态 ,与健康人比较 ,差异有显著性 (t=2 .2 6～ 10 .6 7,P <0 .0 5、0 .0 1)。④结论　TNF α、IL 6及CRP是预测骨感染病人病情危重的重要指标之一 ,对临床有一定指导意义     

脉动真空灭菌器内室气体流向监测报警装置的研制

本文针对XGI型脉动真空灭菌柜出现的蒸汽倒流故障设计出一种内室气体流向监测报警装置，对管道中的蒸汽流向和空气流向进行监测。当出现倒流现象时，报警装置发出报警信号，提醒工作人员采取措施排除故障。经在两台灭菌柜上使用，证明此方案是一种行之有效的方法。     

Treatment of scleromyxoedema with hydroxychloroquine

Background: Scleromyxoedema is a rare disease of unknown aetiology that is characterized by deposition of mucin and sclerotic induration of the skin; it is associated with paraproteinaemia. Patients suffer from progressive disability due to immobilization and cosmetic disfigurement. Treatment of scleromyxoedema is a therapeutic challenge. The antimalarial hydroxychloroquine has a rapid and reliable effect in reticular erythematous mucinosis. Patients and methods: Four consecutive patients (two women, two men; median age: 50 years) with scleromyxoedema, three of them with IgG λ paraprotein, were treated with hydroxychloroquine. Treatment was initiated with 600 mg p. o. for 10 days, followed by 400 mg for at least 4 weeks, and 200 mg thereafter. Results: Complete remission of skin manifestations was achieved in one patient, whereas three patients achieved a partial remission of 61+, 5 and 25 months' duration. Notably, three patients felt increased mobility and reduced firmness of skin during the first week of treatment, which was reflected in a rapid reduction in dermal thickness. In one patient, dysphagia was reverted as evidenced by normalization of oesophageal clearance. Paraproteinaemia was not influenced at all. Side effects included one case of electroretinogram abnormalities after 19 months of therapy and one case of leucopenia after 3 months. Conclusion: Hydroxychloroquine is an effective form of therapy for scleromyxoedema, leading to rapid and prolonged alleviation of symptoms.     

琼脂糖胶/聚苯乙烯磺酸微囊化胰B细胞株MIN6作为人工生物胰的实验研究

应用琼脂糖胶／聚苯乙烯磺酸微囊化胰B细胞林MIN6作为人工生物胰进行大鼠皮下异种移植。结果表明，在不应用任何免疫抑制剂的情况下，大鼠皮下异种移植一周后，重新取出的微囊仍保持光滑完整的球状，其内MIN6细胞仍清晰可见，囊周无明显纤维化及粘着。胰岛素释放试验结果显示，低糖浓度刺激时胰岛素的释放量为19．20±1．35μU·10microbead－1／h；高糖浓度刺激时胰岛素的释放量达到51．47±3．80μU·100microbeads-1／h，约为低糖浓度时的2．7倍，与低糖浓度时比较其差异有高度显著性（P＜0．001）。提示琼脂糖胶／聚苯乙烯磺酸微囊化胰B细胞林MIN6作为人工生物胰，既能保护移植物膜B细胞株MIN6免受排斥反应，也能维护膝B细胞株MIN6对糖刺激的有效的胰岛素分泌反应性。