鞍区颗粒细胞瘤

鞍区颗粒细胞瘤非常罕见,至今报道有症状的颗粒细胞瘤还不足５０例,文章回顾自１８９３发现鞍区颗粒细胞瘤经以来对颗细胞瘤的结构特点、组织起源的研究,以以及临床表现、诊断及鞍区颗粒细胞瘤的治疗     

大肠癌p16抑癌基因甲基化及与Dukes分期的关系

目的 检测大肠癌患者p16基因启动子CpG区甲基化的改变,探讨其与大肠癌发生、发展和临床资料的关系。方法 采用甲基化特异PCR技术（MSP法）研究58例大肠癌标本p16基因甲基化改变,分析临床病理资料。结果 25例标本（43．1％）呈p16 CpG区甲基化阳性;Dukes C、D期病人p16 CpG区甲基化发生率（75％）明显高于Dukes A、B期病人（13．3％）。结论 p16启动子区甲基化导致p16抑癌基因失活参与了大肠癌的发生和发展;p16甲基化可以作为估计大肠癌病人预后的一个指标。     

Primary anorectal malignant melanoma: Report of a case

(Received for publication on Aug. 18, 1997; accepted on May 15, 1998)     

丙型肝炎病毒分片段抗体与HCVRNA的检测分析

目的探讨丙型肝炎病毒(HCV)分片段抗体(HCV-C,HCV-NS3,HCV-NS4,HCV-NS5)对丙肝的诊断价值。方法对ELISA法抗-HCV总抗体阳性的55份阳性血清及80份可疑血清再进行分片段抗体ELISA检测及RT-PCR。结果55份ELISA法抗-HCV总抗体阳性血清经分片段抗体ELISA检测后,抗-NS3、抗-C的检出率最高,分别为96.4%、89.1%;两个以上片段检测结果阳性的血清有54份,与RT-PCR结果(HCVRNA阳性52份)高度符合。80份可疑血清中10份抗-C和抗-NS3同时阳性和9份HCVRNA阳性。结论HCV分片段抗体ELISA法有很高的敏感性和特异性,与RT-PCR法基本一致。抗-NS3、抗-C在HCV的诊断中有重要的意义,抗-NS5和抗-NS4有诊断的互补作用。     

Complexity of the immunoglobulin light chain V kappa 1 gene family in the New Zealand black mouse

The immunoglobulin light chain V kappa 1 gene family is polymorphic in murine inbred strains and this family has been subdivided into five sub-groups (V kappa 1A-E). The V kappa 1A sub-group contributes to approximately 2% of the total serum immunoglobulin light chains in several mouse strains. However, it has been reported that this sub-group is absent in New Zealand Black (NZB) mouse serum. Amino acid sequencing of myeloma proteins from this inbred mouse has shown that they belong to the V kappa 1B sub-group. We report here the structure of nine functional germline genes from NZB mice that have high homologies to the V kappa 1A, V kappa 1B, V kappa 1C, and V kappa 1D sub-groups. In addition, a novel germline gene representing the prototype of a new sub-group (designated V kappa 1F) has been identified. We have isolated different V kappa 1 germline genes from a single restriction fragment length polymorphism (RFLP) fragment, as well as identical V genes from two different RFLP migrating bands. Therefore, the complexity of the genes encoding the immunoglobulin variable region cannot be determined solely by RFLP analysis. Nucleotide sequence analysis of 16 V kappa 1 genes which code for NZB autoantibodies indicate that they belong to five different V kappa 1 sub-groups with five hybridomas (31%) expressing the V kappa 1A sub-group. Comparison of the sequences of V kappa 1 genes expressed in hybridomas with corresponding germline genes show no somatic mutations.     

药物经济学在亚太地区的发展与应用

目的:为我国药品相关政策制订提供借鉴。方法:介绍药物经济学在亚太地区的发展并比较其在药品定价及补偿相关政策 中的应用。结果与结论:药物经济学可作为药品定价和补偿的参考依据。     

肝针吸良、恶性细胞核内AgNORs图像分析的定量研究

应用真彩色图像分析技术对肝针吸细胞中的２０例肝癌及５例增生肝细胞核内ＡｇＮＯＲｓ进行了定量研究。结果显示，肝针吸肝癌细胞核内ＡｇＮＯＲｓ的平均面积、平均积分光密度、颗粒直径及平均颗粒数均明显高于增生肝细胞（Ｐ＜００１）；ＡｇＮＯＲｓ图像定量分析可为肝针吸癌细胞和增生肝细胞的鉴别诊断提供一种新的定量指标；ＡｇＮＯＲｓ在形态、数量及分布上的不同特征也可作为鉴别两者的重要依据。     

AgNOR技术及CT扫描联合诊断肺癌纵隔淋巴结转移的可行性研究

探讨ＡｇＮＯＲ技术及ＣＴ扫描联合诊断肺癌纵隔淋巴结转移的可行性。应用ＡｇＮＯＲ技术和ＣＴ扫描对３８例肺癌纵隔淋巴结转移情况进行分析。伴淋巴结转移肺癌的ＡｇＮＯＲ／核均数显著高于无淋巴结转移肺癌（Ｐ＜０．０５）。ＣＴ扫描淋巴结短径≥８ｍｍ的１０例肺癌中淋巴结转移７例，此７例肺癌细胞的ＡｇＮＯＲ／核均数为８．９７±０．７２，且ＡｇＮＯＲ颗粒均表现为聚集型。提示以肺癌细胞核内ＡｇＮＯＲ颗粒为聚集型，Ａｇ－ＮＯＲ／核均数≥８，ＣＴ扫描淋巴结短径≥８ｍｍ作为标准诊断肺癌纵隔淋巴结转移有较高的特异性及敏感性。ＡｇＮＯＲ技术及ＣＴ扫描联合诊断肺癌纵隔淋巴结转移有较好的临床应用前景