弓形虫急性感染小鼠肺组织的转录组分析

目的 本研究旨在了解弓形虫感染小鼠肺组织转录组的变化.方法 取感染和未感染弓形虫的小鼠肺组织提取总RNA并制备cDNA文库,采用BGISEQ-500测序平台对文库进行高通量转录组测序.从测序结果中筛选差异表达基因(differentially expressed genes,DEGs),并随机筛选 10个差异表达基因利...     

小鼠出生后嗅球发育的基因表达差异及其特征分析

目的:通过研究小鼠嗅球全基因表达差异来寻找嗅球生后发育基因变化及其特征,为嗅球发育及相关研究提供参考依据。方法:分别取新生及成年野生型小鼠完整嗅球并独立进行RNA-seq分析,对全基因测序结果进行差异表达分析并通过检测成年与新生小鼠嗅球中差异表达基因(DEGs),利用Gene Ontology、DAVID及KEGG等数据库,结合q PCR的实验方法对部分差异表达基因进行验证,进一步分析差异表达基因在生物学上的影响。结果:成年小鼠和新生小鼠嗅球存在大量差异表达基因,其中Tfap2e,Neurod6和Tbr1等基因表达下调,同时Zfp365,Elt4以及Omp等基因表达上调,DEGs参与多种信号通路。结论:小鼠嗅球在出生后仍然处于不断发育的过程当中,这些差异表达基因主要影响细胞内代谢,细胞的分裂、分化、成熟及细胞间信号传递。     

Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

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Pluripotent stem cell models of Blau syndrome reveal an IFN-γ–dependent inflammatory response in macrophages

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RNA expression differences in prostate tumors and tumor-adjacent stroma between Black and White Americans

Prostate cancer (PCa) in Black Americans (BA) is diagnosed at an earlier median age and a more advanced stage than PCa in White Americans (WA). Tumor-adjacent stroma (TAS) plays a critical role in tumorigenesis of prostate cancer. We examined RNA expression in both tumor and TAS of BA compared to WA. After evaluating the geographical ancestry of each sample, preliminary analysis of our own RNA-seq data of 7 BA and 7 WA TAS revealed 1706 downregulated and 1844 upregulated genes in BA relative to WA PCa patients (p_adj < 0.05). An assessment of published RNA-seq data of clinically matched tumor-enriched tissues from 15 BA and 30 WA patients revealed 932 upregulated and 476 downregulated genes in BA relative to WA (p_adj < 0.05). When TAS and tumor epithelial cohorts were compared for the top 2500 statistically significant genes, immune responses were downregulated in BA vs WA TAS, while T cell-exhaustion pathways and the immune checkpoint gene CTLA4 were upregulated in BA vs WA tumors. We found fewer activated dendritic cells in tumor and more CD8 T-cells in TAS of BA versus WA PCa patients. Further characterization of these differences in the immune response of PCa patients of distinct geographical ancestry could help to improve diagnostics, prognostics, and therapy.