Stability of immunophenotypic markers in fixed peripheral blood for extended analysis using flow cytometry

The analysis of whole blood samples by flow cytometry for pharmacodynamic and biomarker assessments in clinical studies has been limited by the necessity to test these samples within a short time frame after blood collection. In most clinical studies, blood specimens are shipped to a centralized testing facility; it is critical to demonstrate specimen stability over a period of time which will encompass the time elapsed between specimen collection and testing. A possible solution to overcome this limitation is the use of a fixative to preserve the cell surface antigen stability in whole blood. We examined the stability of markers for T lymphocytes (CD3, CD4, CD8, CD45RA, and CD45RO), B lymphocytes (CD19), NK cells (CD16 + CD56), activation (CD25 and HLA-DR), chemokine receptors (CCR5 and CXCR3) and skin homing (CLA) in fixed blood over 7 days and used this information to select the markers for global clinical studies. These assays with selected markers were further validated using fit-for-purpose approach (Lee et al., 2006) and to set the sample acceptability criteria for use in clinical sample testing. Most of the markers examined were stable when collected in Cyto-Chex® BCT for one week with the exception of the activation markers on T cells.     

寻常性银屑病肾脏损伤与PASI评分的关系

目的探讨银屑病皮损面积和严重程度与银屑病肾损伤的关系。方法采用Spearman秩对114例银屑病患者进行尿Alb,尿IgG,尿β2-MG与皮肤损害面积和皮肤损伤程度进行相关分析。结果构成PASI的红斑、浸润、鳞屑、面积四项因素均与尿微球蛋白不相关,而4项因素均与尿球蛋白具有相关性,红斑、浸润、鳞屑3项因素与尿白蛋白具有相关性。结论肾小球损伤与PASI相关密切,尤其是红斑与肾小球结构性损伤相关性更为密切。     

刺络拔罐与消银汤联合护理干预银屑病随机平行对照研究

[目的]观察刺络拔罐、消银汤联合护理干预银屑病疗效。[方法]使用随机平行对照方法,将80例门诊患者按抛硬币法简单随机分为两组。对照组40例消银汤(荆芥、当归、白术各10g,白鲜皮、苦参各15g,白蒺藜、地肤子各12g,川芎10g,蛇蜕12g,蝉蜕10g;风盛加蛇床子12g;血热加丹皮、栀子、黄芩各10g;湿毒加苍术、茯苓各10g;血虚血瘀加熟地21g,桃仁10g,益母草15g),1剂/d,水煎150m L,早晚口服。治疗组40例1刺络拔罐:取背部腧穴,分两组:一组为大椎、肺俞、肝俞,另一组为大椎、膈腧、肾俞,8号针头浅刺穴位,后在穴位上拔罐,留罐10~15min,两组穴位交替进行,1次/3d;2护理:a.心理护理;b.疼痛护理;c.饮食指导;d.皮肤护理;消银汤治疗同对照组。连续治疗30d为1疗程。观测临床症状、满意度、PASI、不良反应。连续治疗2疗程,判定疗效。[结果]治疗组痊愈11例,显效16例,有效9例,无效4例,总有效率90.00%。对照组痊愈7例,显效13例,有效7例,无效13例,总有效率67.50%。治疗组疗效优于对照组(P0.01)。满意度治疗组优于对照组(P0.01)。[结论]刺络拔罐、消银汤联合护理干预银屑病银屑效果显著,值得推广。     

耳部割治联合常规中药治疗血热型银屑病临床观察

目的观察耳部割治联合常规中药治疗血热型银屑病的临床疗效。方法选择血热型银屑病患者50例,随机分为治疗组和对照组,每组25例,两组均给予常规中药治疗,治疗组在此基础上进行耳部割治,每星期2次,两组均以3星期为1个疗程,观察患者治疗前后银屑病皮损面积和严重程度指数(PASI)评分的改变。结果两组治疗前后PASI评分组内比较差异均有统计学意义(P0.01);治疗后PASI评分组间比较差异有统计学意义(P0.01)。治疗组愈显率优于对照组,差异有统计学意义(P0.05)。结论耳部割治联合常规中药治疗血热型银屑病疗效确切,且无明显不良反应。