PAN-Rh6G能量转移荧光猝灭法测定水样中痕量镉(Ⅱ)

目的：根据能量转移荧光猝灭程度,建立一种荧光猝灭法测定环境水样中痕量镉的新方法.方法：在无水乙醇介质中,Cd^2＋与1-（2-吡啶偶氮）-2-萘酚（PAN）形成PAN-Cd^2＋配合物,其吸收光谱与罗丹明6G（Rh6G）的发射光谱有效重叠,发生能量转移,使Rh6G荧光猝灭,从而建立了痕量镉的荧光猝灭测定新方法.结果：在优化实验条件下,在31～800 ng/ml浓度范围内,Rh6G荧光猝灭程度与PCd^2＋呈现良好的线性关系（r=0.9999）.方法的检出限为9.0 ng/ml.结论：方法灵敏、快速、简便,用于环境水样中痕量镉的测定,结果满意.     

微型结节性多动脉炎患者血清抗髓过氧化物酶抗体的检测意义

抗髓过氧化物酶（抗ＭＰＯ）抗体是抗中性粒细胞浆抗体（ＡＮＣＡ）中的一种，其对结节性多动脉炎（ＰＡＮ）的诊断价值日益受到重视。我们应用ＥＬＩＳＡ方法对１９例ＰＡＮ患者血清进行检测，６／１９例抗体阳性（３２％），其中５例临床表现合并肺、肾损害，１例合并皮肤、肾脏病变；另外６例中的１例动态监测，当病变处于急性活动期时，抗ＭＰＯ抗体阳性；而病变趋于慢性化及临床症状趋于缓解时，抗ＭＰＯ抗体阴性。此外，对２２     

用于医疗护理的形状可调节舒适性纤维的制备及性能研究

本研究采用具有形状记忆的 SMPU M5560和普通 PAN 两种切片原料,按照不同比例配制一系列纺丝液,采用湿法纺丝方法,制备出兼具形状记忆与一定强度的智能纤维。混合后的纤维强度比纯 SMPU 纤维的强度明显增加,利用 TG 和 DSC 对纤维进行测试,得到的纤维能够保持一定的耐热性及形状记忆功能。这种混合纤维在医疗服装的开发上具有一定的应用价值。     

Multivariate optimization of cloud point extraction procedure for zinc determination in aqueous extracts of medicinal plants by flame atomic absorption spectrometry

Cloud point extraction method has been developed for preconcentration of trace quantities of zinc (Zn) in aqueous extract of medicinal plants and blood samples of liver cancer patients using flame atomic absorption spectrometry. The Zn in aqueous extracts of medicinal plants (MPs) was complexed with 2-methyl-8-hydroxyquinoline (quinaldine) and 1-(2-pyridylazo)-2-naphthol (PAN) separately and entrapped in a non-ionic surfactant Triton X-114. After centrifugation, the surfactant-rich phase was diluted with 0.25 mL acidic ethyl alcohol. The multivariate strategy was applied to estimate the optimum values of experimental variables (pH, time temperature, ligands and surfactant concentrations). Interactions between analytical factors and their optimal levels were investigated by two level factorial designs. Student’s t-test on the results of factorial design with 16 runs for Zn extraction, demonstrated that the factors, ligands concentrations, pH and temperature were statistically significant. The accuracy was assessed by analysis of certified reference materials, namely, BCR 101 (spruce needles), Clincheck control-lyophilized human whole blood. Enhancement factor of 30 and 26 were achieved for the preconcentration of Zn by 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2), respectively. The relative standard deviation for six replicate determinations of Zn at 10 μg/L level using 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2) were <4% and >5%, respectively.     

Multi-glycoside of Tripterygium wilfordii Hook. f. reduces proteinuria through improving podocyte slit diaphragm dysfunction in anti-Thy1.1 glomerulonephritis

Ethnopharmacological relevance

Multi-glycoside of Tripterygium wilfordii Hook. f. (GTW) has been proved clinically effective in reducing proteinuria in chronic kidney disease in China. However, the mechanisms involved are still unclear. In this study we examined the effects of GTW at the different dosages on proteinuria and podocyte slit diaphragm (SD) dysfunction in anti-Thy1.1 glomerulonephritis (GN).

Materials and methods

Rats with anti-Thy1.1 GN were divided into 2 groups, a GTW group and a vehicle group, and sacrificed at 30 min, on day 7, and on day 14 in Experiments 1, 2 and 3, respectively. The administration of GTW at the moderate and high doses was started 3 days before or at the same time of antibody injection till sacrifice. Proteinuria was determined in Experiments 1, 2, and 3. After sacrifice, the staining intensity of SD-associated key functional molecules including nephrin and podocin, podocyte structure, mesangial change, macrophage infiltration, and blood biochemical parameters were examined, respectively. Protein and mRNA expressions of nephrin and podocin in glomeruli were also investigated. Besides, liver histological characteristics were analyzed.

Results

In Experiment 1, GTW pretreatment at the medium dose (75 mg/kg body weight) caused no influence on the induction of anti-Thy1.1 GN and the basal nephrin expression. In Experiment 2, the high dosage (100 mg/kg body weight) of GTW ameliorated proteinuria, the distribution of nephrin and podocin, mesangial proliferation, and the activated macrophage accumulation, as compared with vehicle group (P < 0.05). Additionally, it increased mRNA and protein expressions of nephrin and podocin in glomeruli on day 7, but had no influence on podocyte structure. In Experiment 3, the medium dosage (75 mg/kg body weight) of GTW improved proteinuria, the partial matrix expansion, and the distribution of nephrin and podocin on day 14, as compared with anti-Thy1.1 GN rats (P < 0.05). GTW at the high or moderate dose did not affect hepatic function on day 7 and on day 14.

Conclusions

Podocyte SD dysfunction, such as the disordered distribution and down-regulation of nephrin and podocin expression, is critically involved in the pathogenesis of anti-Thy1.1 GN induced by mAb 1-22-3. The restoration of the distribution and expression of nephrin and podocin by GTW could be an important mechanism by which GTW ameliorates proteinuria and podocyte SD dysfunction.     

超临界CO2方法制备的聚丙烯腈的表面特性

根据Washburn浸渍理论(Penetration theory)和van Oss-Good-Chaudhury的组合理论及应用柱状灯芯技术(Column wicking technique)，测试了用超临界CO2方法制备的不同分子量聚丙烯腈(PAN)的表面特性。结果显示，PAN的分子量影响其表面性能。与一般聚合物的表面能随分子量增大而增大的规律不同，超临界方法制备的聚丙烯腈(PAN)的表面能随着分子量的增大而降低，但其Lifshitz-van der Waals力却随分子量的增大而增大。与常规方法生产的PAN的表面性能比较，超临界方法可能使PAN的极性减少。     

潘善余治疗慢性咳嗽经验举隅

[目的] 总结潘善余主任治疗慢性咳嗽的思路与经验。[方法] 通过跟师临证,从如何辨病论治,如何应用清热、治痰、补虚的方法等方面,总结潘师治疗慢性咳嗽的临床经验及方药特色,并附验案一则以佐证。 [结果] 潘师在临床诊治中,常结合现代医学知识,根据各种慢性咳嗽的特征及其伴随症状,辨病论治;常采用清热解毒的方法治疗痰热咳嗽;重视行气化痰和祛痰豁痰排痰法的应用;对反复发作的咳嗽,常祛邪与益肺、健脾、补肾纳气相结合。所举验案辨为风邪缠喉、痰热郁肺证,先治以清热宣肺化痰、祛风利咽通窍;后根据病情转变,及时结合益气固卫法,取得较好疗效。[结论] 潘师治疗慢性咳嗽,结合现代医学知识,辨病论治;应用清热、治痰、补虚的方法辨证施治,效果明显,值得临床推广。