量子点对口腔鳞癌Tca8113活细胞中bcl-2、bax的荧光标记观察

目的:探讨量子点对口腔鳞癌(OSCC)活细胞bcl-2、bax蛋白进行观察研究。方法:量子点QD605,QD545通过间接免疫荧光法,在激光共聚焦显微镜下观测人舌癌Tca8113活细胞内bcl-2、bax蛋白与量子点孵育0.5 h、1.0h、1.5 h和2.0 h的荧光成像。结果:量子点对OSCC活细胞中的bcl-2、bax蛋白的观察研究显示:在0.5 h～2.0 h时,QD605和QD545与bcl-2、bax蛋白结合,荧光从分布于胞浆边缘逐渐至胞浆。结论:量子点能对活细胞内蛋白进行观察。     

舌正中岛状瓣修复口底缺损

目的：总结应用舌正中岛状瓣修复口底缺损的经验。方法：5例中小型口底缺损患者采用舌正中岛状瓣修复重建。在舌背正中部位制备舌肌黏膜瓣,通过切开舌尖或舌组织隧道以岛状瓣的方式转移修复口底缺损。结果：5例患者术后舌瓣均成活良好,无舌瓣坏死,1例术后一侧舌尖部分坏死。术后随访3-6个月,患者发音及吞咽功能无明显异常。结论：舌正中岛状瓣是一种修复中小型口底缺损的好方法。     

The comparative evaluation of the effects of tongue cleaning on salivary levels of mutans streptococci in children

To cite this article:
Int J Dent Hygiene 10 , 2012; 107–112
DOI: 10.1111/j.1601‐5037.2011.00522.x Rupesh S, Winnier JJ, Nayak UA, Rao AP, Reddy V, Peter J. The comparative evaluation of the effects of tongue cleaning on salivary levels of mutans streptococci in children. Abstract: Objectives: The study compared and evaluated the effects of tongue scraping and tongue brushing on salivary mutans streptococci levels in children. Methods: The investigation was a single‐blind, stratified comparison of three parallel groups of children who performed either tongue scraping or tongue brushing along with tooth brushing or only tooth brushing twice daily under professional supervision for a 21‐day period. A total of three saliva samples were taken from each individual, at baseline, on day 10 and on day 21, and colony counts of mutans streptococci were determined. All data were subjected to statistical analysis using Wilcoxon’s signed‐ranks sum test and Mann–Whitney U‐test. Results and conclusions: The results of the present study show that tongue scraping and tongue brushing demonstrated statistically significant reductions in salivary mutans streptococci counts after 10 days and also after 21 days. It was also noted that tongue scraping and tongue brushing were equally effective in reducing colony counts.     

面动脉岛状肌皮瓣修复舌癌术后组织缺损12例

目的:探讨下颌下区面动脉岛状肌皮瓣修复舌癌根治术后组织缺损的可靠性及效果。方法 :选择2009-01—2012-01,韶关市第一人民医院口腔颌面外科收治的舌癌患者12例,其中男性7例,女性5例,全部采用下颌下区面动脉岛状肌皮瓣同期修复舌癌根治性切除术后半舌缺损,术后观察组织瓣成活情况、外形和功能、患者的生存状态以及癌瘤复发等情况。结果:12例患者中,皮瓣完全成活11例,1例皮瓣部分坏死。术后随访6～36个月,患者舌部皮瓣形态、质地良好,语言及吞咽功能理想。1例患者出现颈部肿瘤复发。结论:下颌下区面动脉岛状肌皮瓣可作为半舌缺损修复较理想的皮瓣,该术式简单,易成功,适合同期修复舌癌根治术后半舌缺损。     

颏下岛状皮瓣在舌癌术后组织缺损修复中的临床应用

目的：总结颏下岛状皮瓣修复舌癌术后组织缺损的临床经验。方法：对我科收治的11例舌癌术后缺损患者进行颏下岛状瓣修复,其中男性7例,女性4例,全部病例行舌癌扩大切除术,舌缺损采用颏下动脉岛状皮瓣同期修复。术后观察组织瓣生长情况以及修复效果。结果：10例皮瓣存活,皮瓣形态自然美观,舌活动灵活,基本恢复解剖形态和生理功能。术后随访12个月,未发现复发和转移。1例男性患者组织瓣上有胡须生长。1例出现皮瓣远端部分坏死,二期愈合。结论：应用颏下岛状皮瓣修复舌癌术后缺损操作简单,修复效果良好,是一种有效的修复方法。     

Spontaneous electromyographic activity of the tongue in amyotrophic lateral sclerosis

Introduction: Detection of denervation in muscles in the craniobulbar area is important to assure widespread lower motor neuron involvement in the diagnosis of amyotrophic lateral sclerosis (ALS). The value of spontaneous activity analysis in needle electromyography (EMG) of the tongue has been questioned in the recent literature. Methods: Spontaneous activity in the tongue and sternocleidomastoid (SCM) muscles was reviewed retrospectively in 17 ALS patients. Results: Needle EMG showed spontaneous activity in the tongue in 14 of 17 patients (82%) and in 6 patients of 17 (35%) in SCM. Spontaneous EMG activity in the tongue was found in patients with and without bulbar symptoms. Conclusions: Needle EMG is a valuable method for assessing clinical and subclinical involvement of the tongue in patients with bulbar and limb onset ALS. Adequate relaxation of the tongue is a prerequisite for proper spontaneous activity recording. Muscle Nerve, 48: 296–298, 2013     

An electropalatographic investigation of linguopalatal contact in participants with acquired apraxia of speech: A quantitative and qualitative analysis

The current study aimed to provide a comprehensive analysis of linguopalatal contact patterns in participants with acquired apraxia of speech (AOS). Tongue‐to‐palate contacts were recorded for three participants with AOS during consonant singletons and consonant clusters using the Reading Electropalatograph (EPG3) system. Amount and pattern of linguopalatal contact were analysed using qualitative and quantitative analysis methods and the degree of spatial variability was quantified. The results obtained for each of the apraxic speakers were individually compared to those obtained by a control group (n = 5). Misdirected articulatory gestures and distorted spatial configurations for alveolar fricatives were detected in the linguopalatal contact patterns recorded for each of the participants with AOS. Lingual overshoot, right‐side dominant contact, and increased spatial variability were also evidenced by some participants. True omission errors were absent. Overall, the combination of qualitative and quantitative analysis techniques proved valuable in providing a comprehensive analysis of linguopalatal contact in AOS. Results provided support for a motoric account of articulatory and prosodic deficits in AOS.