Synthesis and Electrochemical Performance of V6O13 Nanosheets Film Cathodes for LIBs

V₆O₁₃ thin films were deposited on indium-doped tin oxide (ITO) conductive glass by a concise low-temperature liquid-phase deposition method and through heat treatment. The obtained films were directly used as electrodes without adding any other media. The results indicate that the film annealed at 400 °C exhibited an excellent cycling performance, which remained at 82.7% of capacity after 100 cycles. The film annealed at 400 °C with diffusion coefficients of 6.08 × 10⁻¹² cm²·s⁻¹ (Li⁺ insertion) and 5.46 × 10⁻¹² cm²·s⁻¹ (Li⁺ extraction) in the V₆O₁₃ film electrode. The high diffusion coefficients could be ascribed to the porous morphology composed of ultrathin nanosheets. Moreover, the film endured phase transitions during electrochemical cycling, the V₆O₁₃ partially transformed to Li_0.6V_1.67O_3.67, Li₃VO₄, and VO₂ with the insertion of Li⁺ into the lattice, and Li_0.6V_1.67O_3.67, Li₃VO₄, and VO₂ partially reversibly transformed backwards to V₆O₁₃ with the extraction of Li⁺ from the lattice. The phase transition can be attributed to the unique structure and morphology with enough active sites and ions diffusion channels during cycles. Such findings reveal a bright idea to prepare high-performance cathode materials for LIBs.     

Influence of Tempering Temperature and Time on Microstructure and Mechanical Properties of Additively Manufactured H13 Tool Steel

Among various processes for manufacturing complex-shaped metal parts, additive manufacturing is highlighted as a process capable of reducing the wastage of materials without requiring a post-process, such as machining and finishing. In particular, it is a suitable new manufacturing technology for producing AISI H13 tool steel for hot-worked molds with complex cooling channels. In this study, we manufactured AISI H13 tool steel using the laser power bed fusion (LPBF) process and investigated the effects of tempering temperature and holding time on its microstructure and mechanical properties. The mechanical properties of the sub-grain cell microstructure of the AISI H13 tool steel manufactured using the LPBF process were superior to that of the H13 tool steel manufactured using the conventional method. These sub-grain cells decomposed and disappeared during the austenitizing process; however, the mechanical properties could be restored at a tempering temperature of 500 °C or higher owing to the secondary hardening and distribution of carbides. Furthermore, the mechanical properties deteriorated because of the decomposition of the martensite phase and the accumulation and coarsening of carbides when over-tempering occurred at 500 °C for 5 h and 550 °C for 3 h.     

抑制miR-204表达对宫内发育迟缓新生大鼠学习记忆能力的影响及相关机制

目的探讨抑制miR-204表达对宫内发育迟缓（intrauterine growth restriction，IUGR）新生大鼠学习记忆能力的影响及机制。方法采用低蛋白饮食法建立IUGR大鼠模型，将3日龄IUGR幼鼠设为模型组、miRNA拮抗剂对照组（antagomir-NC组）和miR-204拮抗剂组（antagomir组），另取正常幼鼠设为对照组，每组10只。Morris水迷宫实验测定大鼠学习与记忆能力；qRT-PCR检测各组大鼠海马组织中miR-204和脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）mRNA表达水平；尼氏染色、Tunel法观察海马组织尼氏小体数量和细胞凋亡水平；Western blot检测海马组织中BDNF/TrkB信号通路相关蛋白表达水平。结果与对照组比较，模型组大鼠逃避潜伏期增加，跨台次数减少；海马组织中细胞凋亡率和miR-204表达水平升高，而尼氏小体数量、BDNF mRNA，以及BDNF、p-TrkB和p-CREB蛋白表达水平降低（P<0.001）。抑制miR-204表达后，IUGR大鼠海马组织中尼氏小体数量、BDNF mRNA，以及BDNF、p-TrkB和p-CREB 蛋白表达水平升高，细胞凋亡率和miR-204表达水平降低，逃避潜伏期减少，跨台次数增加（P<0.001）。结论抑制miR-204可改善IUGR新生大鼠学习及记忆功能，其作用机制可能与靶向激活BDNF/TrkB信号通路有关。     

Apelin-13对高尿酸诱导的脂肪细胞氧化应激的作用

目的:探讨apelin-13对高尿酸诱导的3T3-L1脂肪细胞氧化应激的作用及其机制。方法:3T3-L1脂肪细胞予以10 mg/dL尿酸刺激,部分细胞予以1μmol/L apelin-13预处理,以100μmol/L H₂O₂刺激的细胞为阳性对照。48 h后,流式细胞术检测活性氧族(ROS)含量,生化试剂盒检测细胞及培养液上清抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)]和促氧化酶NADPH氧化酶(NOX)的活性以及丙二醛(MDA)含量,real-time PCR法检测细胞局部肾素-血管紧张素系统(RAS)各组份血管紧张素原(AGT)、血管紧张素转化酶I(ACE1)、血管紧张素II 1型受体(AT1R)和AT2R及血管紧张素II 1型受体相关蛋(APJ)的mRNA表达水平,ELISA法检测细胞及培养液中血管紧张素II(AngⅡ)浓度。结果:10 mg/dL的尿酸明显降低3T3-L1脂肪细胞SOD、GSH-Px和CAT的活性,升高NOX的活性,增加MDA的含量,细胞内ROS的含量相应升高;apel...     

极化转移INEPT与DEPT以及季碳脉冲序列等方法在~(13)CNMR测定中的应用

NMR测定灵敏度较低,对于那些天然丰度低而磁矩又较小的核,这一问题更为突出。70年代末80年代初,出现了一系列基于极化转移技术的多脉冲序列方法如INEPT~*和DEPT~(**)等,用于测定~(18)CNMR谱,极大地提高了灵敏度,其增强因子与偶极—偶极弛豫是否     

重组人骨形成蛋白-2在诱导C3H10T1/2干细胞成骨中对MMP-9 mRNA表达的影响

目的：测定MMP-9mRNA在C3H10T1／2 clone 8(10T1／2)中的表达,探讨rhBMP-2诱导10T1／2产生成骨细胞时对MMP-9mRNA表达的影响。方法：取10T1／2培养至细胞完全贴壁,加入rhBMP-2培养28天,同时利用Real—time PCR检测MMP-9在10T1／2中不同时间段的表达。结果：10T1／2经rhBMP-2的诱导14天出现钙化点,MMP-9mRNA也随着rhBMP-2的加入表达降低,而对照组几乎不变化。结论：rhBMP-2在诱导10T1／2产生成骨细胞的同时抑制着MMP-9mRNA的表达。     

Factors associated with patient activation in a socially deprived population: Evidence from Germany

ObjectivePatient activation has been identified as a crucial determinant of health, but little is known about its own determinants, particularly in low socioeconomic status populations. To address this research gap, we analyzed factors that might explain variation in patient activation in such a population.MethodsWe conducted a cross-sectional patient survey (n = 582) in a low socioeconomic status urban district in Germany in 2017. Using multivariate linear regressions, we examined the association between patient activation and a range of psychological, sociodemographic, and health-related factors. To assess the relative importance of these factors, we used dominance analysis.ResultsOur results suggest that age, employment status, linguistic acculturation, health status, and self-efficacy were significantly associated with patient activation. Dominance analysis indicated that self-efficacy was the most important factor explaining variation in patient activation.ConclusionsAge, employment status, linguistic acculturation, health status, and self-efficacy are important determinants of patient activation.Practice implicationsOur results can inform decision makers about approaches for more targeted and effective interventions to improve patient activation in low socioeconomic status populations. Much might be gained by investing in interventions that focus on age, employment status, linguistic acculturation, and health status. Interventions that improve self-efficacy may represent a particularly promising approach.     

1990年和2010年中国3种细菌性脑膜炎疾病负担情况

分析上海市13价肺炎球菌多糖结合疫苗（PCV13）单独接种和与其他疫苗联合接种后的安全性。

通过中国免疫规划监测信息管理系统所收集的疑似预防接种异常反应（AEFI）中被动监测资料和上海市预防接种信息系统中接种PCV13的婴幼儿基本信息和疫苗使用资料,分析PCV13单独接种和同时与其他疫苗接种的AEFI报告发生率。

PCV13单独接种892 329剂次,AEFI报告11 175例,AEFI报告发生率为1 252.34/10万;PCV13联合接种14 769剂次,AEFI报告215例,AEFI报告发生率为1 455.75例/10万。与PCV13联合接种最多的疫苗是口服轮状病毒减毒活疫苗（ORV）,AEFI报告发生率为1 706.67/10万。PCV13单独接种和联合接种后AEFI报告均发热症状比例最高,报告发生率分别为876.69/10万和1 442.21/10万。

相对单独接种,PCV13与其他部分疫苗联合接种增加了AEFI一般反应报告发生率,但尚可接受。

     

人工全膝关节置换术中联合股骨外髁滑移截骨术矫正股骨外弓畸形的疗效分析

目的探讨人工全膝关节置换术（total knee arthroplasty，TKA）中采用股骨外髁滑移截骨术（lateral condyle sliding osteotomy，LCSO）矫正股骨外弓畸形的疗效。方法回顾分析2018年7月—2020年7月TKA中采用LCSO治疗的17例伴股骨外弓畸形的骨关节炎患者临床资料。男3例，女14例；年龄58～68岁，平均63.2岁。股骨外弓畸形病因：股骨发育畸形12例，股骨骨折畸形愈合5例。膝关节骨关节炎Kellgren-Lawrence分级：Ⅲ级4例，Ⅳ级13例。术前生理外翻角为9.5°～12.5°，平均10.94°。病程3～25年，平均15.1年。术前及末次随访时测量股骨远端机械外侧角（mechanical lateral distal femur angle，mLDFA）、髋-膝-踝角（hip-knee-ankle angle，HKA）、机械轴偏向（mechanical axis deviation，MAD），评估关节外畸形在关节内矫正及下肢机械力线恢复情况；采用膝关节学会评分系统（KSS）膝评分和功能评分、疼痛视觉模拟评分（VAS）、膝关节活动度（range of motion，ROM）评估疗效；行膝内、外翻应力试验，X线片复查截骨片愈合情况，评估关节稳定性及LCSO的安全性。结果术后患者切口均Ⅰ期愈合，无切口感染、下肢深静脉血栓形成等术后早期并发症发生。17例患者均获随访，随访时间12～36个月，平均23.9个月。截骨片均达骨性愈合，愈合时间2～5个月，平均3.1个月。术后膝内、外翻应力试验均为阴性，未发生外侧副韧带松弛、断裂，膝关节不稳，假体松动、翻修、感染等情况。末次随访时mLDFA、HKA、MAD及膝关节ROM、VAS评分、KSS膝评分和功能评分均较术前显著改善，差异有统计学意义（P<0.05）。 结论在伴有股骨外弓畸形TKA中应用LCSO疗效确切且安全，关节外畸形在关节内矫正，一次手术可同时恢复下肢机械力线和关节平衡。