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991.
目的探讨血小板内皮细胞粘附分子(platelet endothelial cell adhesion molecule-1,PECAM-1)基因G+1688A(Ser563Asn)多态性与老年人群心肌梗死(myocardial infarction,MI)的关系。方法采用病例-对照研究,应用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)分析技术对100例老年MI患者和120例老年非冠心病对照人群PECAM-1基因的第8号外显子区单碱基突变多态性(single nucleotide polymorphism,SNP)进行分析,即基因型和等位基因频率在两组中的分布规律及其与MI的关系。结果AA基因型频率在病例组与对照组之间有显著差异(43%:24%,p<0.001),等位基因间亦存在同样的趋势(A/G:65%:54%,p<0.001)。而且在高胆固醇或收缩压过高的人群中,基因型为AA者患MI的危险度增高。结论在老年MI患者中,PECAM-1基因G+1688A点突变多态性与MI发病密切相关,可能是老年患者MI的遗传危险因素。 相似文献
992.
BackgroundMyocardial infarction (MI), caused by temporary or permanent coronary artery occlusion, poses a serious threat to patients’ lives. Circular RNAs (circRNAs), a new kind of endogenous noncoding RNAs, have been widely studied recently. This study was designed to illustrate and potential molecular mechanisms of circRNA 010567 in hypoxia-induced cardiomyocyte injury in vitro, so as to provide new strategies for the therapy of MI.MethodsH9c2 cells were cultured in anoxic conditions with 94% N2, 5% CO2, and 1% O2 to establish the in vitro MI model. Cell viability and apoptosis were checked using MTT and flow cytometry assay, respectively, Moreover, the levels of circRNA 010567, miR-141, and DAPK1 was determined using qRT-PCR. The putative targets of circRNA 010567 and miR-141 were confirmed by dual-luciferase reporter system and the RNA immunoprecipitation (RIP) assay. The release of creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), and the viability of mitochondria were detected using assay kits.ResultsThe current study revealed that circRNA 010567 and DAPK1 were over-expressed, and miR-141 was low-expressed in hypoxia-induced MI. circRNA 010567 sponges miR-141 and DAPK1 was a direct target of miR-141. Mechanistic investigations revealed that circRNA 010567-siRNA impaired the release of CK-MB and cTnI, and promoted the viability of mitochondria in hypoxia-induced H9c2 cells, while these findings were reversed by the miR-141 inhibitor. In addition, the miR-141 mimic markedly reduced the release of CK-MB and cTnI, and promoted the viability of mitochondria, and these results were reversed by the DAPK1-plasmid. Subsequently, functional experiments revealed that hypoxia-stimulated decreases in H9c2 cell viability, as well as increases in apoptosis and caspase-3 activity, were induced by the miR-141 mimic and circRNA 010567-siRNA. However, these results were reversed by the miR-141 inhibitor and DAPK1-plasmid.ConclusionsOur results demonstrated that circRNA 010567-siRNA played a protective role in hypoxia-induced cardiomyocyte damage via regulating the miR-141/DAPK1 axis, indicating that circRNA 010567-siRNA may be a promising target for MI therapy. 相似文献
993.
994.
抑制COX-2表达对心肌缺血再灌注损伤过程中心肌细胞保护作用的研究 总被引:1,自引:0,他引:1
目的研究心肌缺血再灌注损伤过程中,环氧化酶-2(COX-2)表达水平与心肌细胞损伤之间的分子机制。方法通过缺氧/复氧(H/R)实验,模拟心肌缺血再灌注过程,在H9C2心肌细胞系中检测COX-2及其相关蛋白的表达水平,并通过COX-2特异性抑制剂NS398抑制其活性,观察细胞变化情况,并研究其作用机制。结果NFκB的激活诱导COX-2表达上调,COX-2促进H/R介导的促炎症因子转录,以及ROS活性增强。结论心肌细胞受到H/R刺激时,NFκB的激活诱导COX-2表达上调,抑制COX-2活性能够降低H/R诱导的促炎症因子转录以及ROS活性增强,从而发挥对心肌细胞的保护作用。 相似文献
995.
Suprat Saely Wilson Gregory M. Kwiatkowski Scott R. Millis John D. Purakal Arushi P. Mahajan Phillip D. Levy 《The American journal of emergency medicine》2017,35(1):126-131
Objectives
The purpose of this study was to compare health care resource utilization among patients who were given intravenous nitroglycerin for acute heart failure (AHF) in the emergency department (ED) by intermittent bolus, continuous infusion, or a combination of both.Methods
We retrospectively identified 395 patients that received nitroglycerin therapy in the ED for the treatment of AHF over a 5-year period. Patients that received intermittent bolus (n = 124) were compared with continuous infusion therapy (n = 182) and combination therapy of bolus and infusion (n = 89). The primary outcomes were the frequency of intensive care unit (ICU) admission and hospital length of stay (LOS).Results
On unadjusted analysis, rates of ICU admission were significantly lower in the bolus vs infusion and combination groups (48.4% vs 68.7% vs 83%, respectively; P < .0001) and median LOS (interquartile range) was shorter (3.7 [2.5-6.2 days]) compared with infusion (4.7 [2.9-7.1 days]) and combination (5.0 [2.9-6.7 days]) groups; P = .02. On adjusted regression models, the strong association between bolus nitroglycerin and reduced ICU admission rate remained, and hospital LOS was 1.9 days shorter compared with infusion therapy alone. Use of intubation (bolus [8.9%] vs infusion [8.8%] vs combination [16.9%]; P = .096) and bilevel positive airway pressure (bolus [26.6%] vs infusion [20.3%] vs combination [29.2%]; P = .21) were similar as was the incidence of hypotension, myocardial injury, and worsening renal function.Conclusions
In ED patients with AHF, intravenous nitroglycerin by intermittent bolus was associated with a lower ICU admission rate and a shorter hospital LOS compared with continuous infusion. 相似文献996.
【目的】探讨不孕症妇女宫颈黏液抗精子抗体 (AsAb)产生与生殖道解脲支原体 (UU )感染的关系。【方法】对 137例女性不孕患者宫颈黏液的AsAb采用酶联免疫法 (ELISA)进行检测 ,使用RocheLightCy cle荧光定量PCR检测仪对标本进行UU检测。【结果】137例不孕症患者中 ,发现抗精子抗体阳性组解脲支原体检出率为 6 9.4 % ( 5 0 / 72 ) ,抗精子抗体阴性组解脲支原体检出率为 32 .3% ( 2 1/ 6 5 ) ,两组之间差异有显著性 ( P <0 .0 5 )。对抗精子抗体、解脲支原体均为阳性组进行了两种治疗方法的疗效对比 ,结果表明 ,使用抗生素组抗体转阴率为 73% ,未使用抗生素组抗体转阴率为 37.5 % ,两组疗效差异有显著性 (P <0 .0 5 )。【结论】AsAb的产生与生殖道感染UU有密切的关系 ,后者可能是引起女性免疫性不孕的重要因素。积极治疗生殖道感染有助于降低AsAb滴度 ,阻止AsAb形成 ,对免疫性不育的治疗有重要意义 相似文献
997.
998.
Peter Kellman Anthony H Aletras Christine Mancini Elliot R McVeigh Andrew E Arai 《Magnetic resonance in medicine》2007,57(5):891-897
T2-weighted MRI of edema in acute myocardial infarction (MI) provides a means of differentiating acute and chronic MI, and assessing the area at risk of infarction. Conventional T2-weighted imaging of edema uses a turbo spin-echo (TSE) readout with dark-blood preparation. Clinical applications of dark-blood TSE methods can be limited by artifacts such as posterior wall signal loss due to through-plane motion, and bright subendocardial artifacts due to stagnant blood. Single-shot imaging with a T2-prepared SSFP readout provides an alternative to dark-blood TSE and may be conducted during free breathing. We hypothesized that T2-prepared SSFP would be a more reliable method than dark-blood TSE for imaging of edema in patients with MI. In patients with MI (22 acute and nine chronic MI cases), T2-weighted imaging with both methods was performed prior to contrast administration and delayed-enhancement imaging. The T2-weighted images using TSE were nondiagnostic in three of 31 cases, while six additional cases rated as being of diagnostic quality yielded incorrect diagnoses. In all 31 cases the T2-prepared SSFP images were rated as diagnostic quality, correctly differentiated acute or chronic MI, and correctly determined the coronary territory. Free-breathing T2 prepared SSFP provides T2-weighted images of acute MI with fewer artifacts and better diagnostic accuracy than conventional dark-blood TSE. 相似文献
999.
R. Zaidan M. Al Moallem B. A. Wani A. R. Shameena A. R. Al Tahan A. K. Daif S. Al Rajeh 《European journal of neurology》2003,10(4):367-372
High dose intravenous immunoglobulin (IVG) is increasingly used in a broad range of immune mediated diseases. Thrombosis was exceptionally reported as a complication of this therapy. We describe three cases of thrombotic complications during or soon after IVIG treatment: myocardial infarction in a man and cerebral infarctions in an elderly man, associated with peripheral ischemia in a woman. In addition we review the published cases in the literature and discuss the possible etiologic factors. 相似文献
1000.