抑制食管癌与肺血管内皮黏附的功能性抗体的制备

目的〖HT5\"SS〗： 制备抑制食管癌细胞与肺血管内皮黏附的功能性单克隆抗体,为研究食管癌转移的靶向治疗剂打下基础。〖HT5W〗方法〖HT5\"SS〗： 以人食管癌新鲜组织分离的肿瘤细胞免疫BALB/c小鼠,将免疫小鼠脾细胞与SP2/0细胞融合后,采用活细胞荧光、ELISA、免疫组化、肿瘤细胞内皮细胞黏附实验、Weastern bloting等方法筛选并鉴定抑制肿瘤细胞黏附的功能性单克隆抗体。〖HT5W〗结果〖HT5\"SS〗： 共融合获得了1 134株杂交瘤克隆,在能与食管癌细胞膜反应的486株     

宫腔粘连的介入性超声诊断和治疗

目的　探讨宫腔粘连的介入性超声诊断和治疗的方法。方法　 64例不孕、闭经及周期性腹痛的患者 ,行介入性超声检查并在超声监导下行经宫颈宫腔粘连松解术。结果　经介入性超声诊断宫腔粘连 63例 (98.43 % )。 64例手术中 ,62例手术顺利 ,2例穿孔。随访 42例 ,2 1例症状消失或减轻 ,其中 4例妊娠 ,2例分娩 ;13例分解粘连组织后取出宫内避孕器、残留胎骨及妊娠囊 ;3例复发。结论　介入性超声是诊断和治疗宫腔粘连的有效方法。     

B超及X线检查对判定异常节育环的价值比较

目的 探讨超声、X线透视检查对判定节育环异常的价值。方法 本组对62例病人进行了B超及X线透视检查,其中至少有一种检查结果显示节育环异常。结果 B超检查节育环异常50例,X线透视检查节育环异常41例,B超诊断节育环异常准确率为80．64％,X线透视诊断节育环异常准确率为66．13％。结论 超声、X线透视对节育环异常的判定各有特点,B超检查对判断节育环异常价值更大。     

整合素细胞信号转导通路与肝星状细胞行为

Integrins are the main mediators of the complicated interaction between hepatic stellate cells (HSCs) and extracellular matrix (ECM) components. Signal transduction by integrins is initiated by both occupancy and crosslinking of integrins by ECM components. Focal adhesion kinase (FAK) and mitogenactivated protein kinase (MAPK), which are the downstream molecules, involve in integrin - mediated cellular behaviors. In this review, we briefly summarize the integrin‘ s structure, intracellular signaling cascades and its role in HSC behaviors, such as activation, proliferation and aoontosis.     

胎儿生长迟缓时体内维生素A水平改变对新生儿视力发育的影响

目的　探讨胎儿生长迟缓 (IUGR)时母血、脐血中维生素A(VitA)水平的变化以及对新生儿视力发育的影响。方法　采用微量荧光法测定IUGR组和正常对照组各 30例孕妇母血、脐血血清中VitA含量 ,并对所有新生儿采用闪光视觉诱发电位 (F VEP)检测新生儿视功能发育状况。结果　IUGR组母血、脐血中VitA含量均低于正常对照组 (P <0 0 1) ,且母血、脐血中VitA含量两者之间相关性 ,r =0 80 99、P <0 0 1;母血、脐血中VitA含量与新生儿体重呈正相关 (P <0 0 1)。F VEP检测IUGR组P10 0潜伏期较正常对照组延长 (P <0 0 5 ) ,而幅值则低于对照组 (P <0 0 1) ;P10 0潜伏期与新生儿体重呈负相关 ,而幅值与新生儿体重呈正相关。结论　提示IUGR时小于胎龄儿 (SGA)视觉发育受到影响 ,该影响发生与母血、脐血中VitA含量下降有关     

妊娠期巨细胞病毒感染的诊断、处理和咨询策略

本文描述了妊娠期不同类型巨细胞病毒(cytomegalovirus,CMV)感染的宫内感染发生率,如何鉴别活动性感染的类型以及如何诊断胎儿发生宫内感染的策略.明确指出妊娠期CMV活动性感染时,需结合胎儿是否感染和胎儿影像学检查结果为患者提供咨询,不能仅因为孕妇CMV IgM和IgG阳性而终止妊娠,还应鼓励母乳喂养.     

宫腔镜宫腔粘连分离术后子宫内膜对不同剂量雌激素的反应及其与生殖预后改善的关系

目的:探讨宫腔镜宫腔粘连分离术(TCRA)后子宫内膜对不同剂量雌激素的反应与生殖预后改善之间的关系.方法:选择2016年1月至2020年1月就诊于宁夏医科大学总医院妇科宫腔镜中心确诊并接受以TCRA手术为主的综合治疗的中、重度宫腔粘连(IUA)患者163例为研究对象,其中,IUA中度114例,IUA重度49例;每组内术...     

腰间盘突出物上摘除切式的改进及其意义

目的：搪塞腰间盘突出症术后腰膜囊、神经根前方粘连受突出物上切口的影响。方法：对１１７例患者采用改良和传统两种切式,并经肉眼观察、比较。结果：切口改进后,局部光滑,无宽大腔隙与会传统切式不同。结论：改进切口对预防或减轻硬膜囊前方粘连可能有一定价值。     

Effect of early nutrition on intestine development of intrauterine growth retardation in rats and its correlation to leptin

AIM: To investigate the intestine and body development of intrauterine growth retardation (IUGR) rats under early different protein diet and to analyze the correlation between leptin and intestine and body development. METHODS: An IUGR rat model was established by food restriction of pregnant female rats. Fifty-six neonatal IUGR rats and 24 neonatal normal rats were randomly divided into normal control group (C group), IUGR model group (SC group), low protein diet IUGR group (SL group), and high protein diet IUGR group (SH group). Eight rats were killed per group at wk 0, 4, and 12. Serum leptin, body weight (BW), body length (BL), intestinal weight (IW), intestinal length (IL), and intestinal disaccharidase (including lactase, maltase, and saccharase) were detected. RESULTS: BW (4.50±0.41 g), BL (5.96±0.40 cm), IW (0.05±0.01 g), and IL (15.9±2.8 cm) in neonatal IUGR rats were much lower than those in C group (6.01±0.55 g, 6.26±0.44 cm, 0.10±0.02 g, 21.8±2.7 cm, P<0.05), while intestinal lactase and maltase activities were higher than those in C group. SH group showed the fastest catch up growth and their BW, BL, IW, and IL reached the C group level at wk 4. SC group showed relatively slower catch up growth than SH group, and their BW, BL, IW did not reach the C group level at wk 4. SL group did not show intestine and body catch up growth. Intestinal maltase [344±33 μmol/(min·g)] and saccharase activities [138±32 μmol/(min·g)] in SL group were both markedly lower than those in C group [751±102, 258±27 μmol/(min·g), P<0.05]. There were no significant differences in lactase activities at wk 4 and disaccharidase activities at wk 12 among all groups (P>0.05). The leptin level in SL. group (0.58±0.12 ng/mL) was the highest in all groups, and much lower in SH group (0.21±0.03 ng/mL) than that in any other IUGR groups at wk 4 (P<0.05). Leptin was negatively related to BW (r=-0.556, P= 0.001), IW (r=-0.692, P=0.001) and IL (r=-0.738, P= 0.000) at wk 4, while no correlation was found at wk 12. CONCLUSION: High protein diet is a reasonable early nutritional mode to IUGR rats in promoting intestine and body catch up growth.     

Social Inequality in Birth Outcomes in Korea, 1995-2008

Social inequality in adverse birth outcomes has been demonstrated in several countries. The present study examined the separate and joint effects of parental education and work in order to investigate the causal pathways of social class effects on adverse birth outcomes in Korea. The occurrence of low birth weight, preterm births, and intrauterine growth retardation was examined among 7,766,065 births in Korea from 1995 to 2008. The effect of social inequality, as represented by parental education and work, was examined against adverse birth outcomes using multivariate logistic regression after controlling for other covariates. Parental education had the most significant and greatest effect on all three adverse outcomes, followed by parental work and employment, which had lesser effects. For adverse birth outcomes, the gap between educational levels increased steadily in Korea from 1995 to 2008. Throughout the analysis, the effect of maternal manual work on adverse birth outcomes was apparent in the study results. Given this evidence of social inequality in education and employment, social interventions should aim at more in-depth and distal determinants of health.