Reliability of cardiovascular reactivity to stress: internal consistency

Research on the reliability of cardiovascular reactivity has focused on temporal stability and intertask consistency with only modest results. The present study evaluated the internal consistency reliability of cardiovascular reactivity in three large samples of adolescents and young adults (N=326, 136, and 142). Impedance cardiographic and blood pressure measures were recorded at rest and during standard laboratory stress tasks (math, video game, cold pressor). The reliability of cardiovascular reactivity within tasks, as assessed by Cronbach's alpha coefficient of internal consistency, ranged from alpha=.83 to .96 for 4-min to 5-min math tasks, and alpha=.65 to .94 for 3-min video game and cold pressor tasks. Although highly reliable within tasks, cardiovascular reactivity was less reliable across tasks, even within a single testing session. Possible reasons for the discrepancy between internal consistency and intertask consistency are discussed.     

The effect of 5-HTTLPR and a serotonergic multi-marker score on amygdala,prefrontal and anterior cingulate cortex reactivity and habituation in a large,healthy fMRI cohort

Major depressive disorder (MDD) is characterized by low mood for at least two weeks. Impaired emotion regulation has been suggested to be the consequence of dysfunctional serotonergic regulation of limbic and prefrontal regions, especially the amygdala, the anterior cingulate cortex (ACC) and the prefrontal cortex (PFC). The impact of genetic variation on brain function can be investigated with intermediate phenotypes. A suggested intermediate phenotype of MDD is emotion recognition: The 5-HTTLPR polymorphism of SLC6A4 as well as other serotonergic genes have been associated with amygdala and prefrontal function during emotion recognition. Previously, it has been suggested that habituation is a more reliable index of emotion recognition than functional activation. We examined the relationship of genes involved in serotonergic signaling with amygdala as well as prefrontal functional activation and habituation during an emotion recognition task in 171 healthy subjects. While effects of 5-HTTLPR and of a serotonergic multi-marker score (5-HTTLPR, TPH1(rs1800532), TPH2(rs4570625), HTR1A(rs6295) and HTR2A(rs6311)) on amygdala activation did not withstand correction for multiple regions of interest, we observed a strong correlation of the multi-marker score and habituation in the amygdala, DLPFC, and ACC. We replicated a well-studied intermediate phenotype for association with 5-HTTLPR and provided additional evidence for polygenic involvement. Furthermore, we showed that task habituation may be influenced by genetic variation in serotonergic signaling, particularly by a serotonergic multi-marker score. We provided preliminary evidence that PFC activation is an important intermediate phenotype of MDD. Future studies are needed to corroborate the results in larger samples.     

Multi-walled carbon nanotube-induced inhalation toxicity: Recognizing nano bis-demethoxy curcumin analog as an ameliorating candidate

Human beings and ecosystems are being possibly exposed to CNTs, as there is a rise in global production rate of carbon nanotubes (CNTs). This may affect the health of humans and increases the environmental risk. We have already reported the pulmonary toxicity due to the inhalation of MWCNTs. We claim that a compound with anti-inflammatory and antioxidant activity may ameliorate the CNT-induced toxic effect. With this view, we have investigated the ameliorative effect of intravenously-administered nano bis-demethoxy curcumin analog (NBDMCA) against MWCNTs-induced inhalation toxicity by examining the lung histopathology for inflammatory cell dynamics, pulmonary remodeling and estimating the inflammatory biomarkers in the broncho-alveolar lavage fluid. We observed that NBDMCA could ameliorate the injury as evidenced by the decline in the levels of markers of inflammation, cell damage, and the histopathological changes induced by MWCNTs. We conclude that NBDMCA may be used to reduce the risk of MWCNTs-induced inhalation toxicity.     

蒲翘异功汤联合四联疗法治疗脾胃虚寒证幽门螺杆菌相关性胃溃疡临床研究

目的:观察蒲翘异功汤联合常规含铋剂四联疗法治疗脾胃虚寒证幽门螺杆菌(Hp)感染相关性胃溃疡的临床疗效。方法:选择符合纳入标准与排除标准的消化科患者60例,将其按随机数字表法分为2组各30例,对照组予四联疗法治疗14天,后予序贯服用予艾司奥美拉唑镁肠溶片治疗6周;观察组在对照组治疗方案的基础上加用蒲翘异功汤治疗。2组均治疗8周评价疗效。疗程结束后观察临床疗效指标及安全性指标。对胃镜下治愈并根除Hp的患者随访4个月,评价其远期疗效。结果:治疗后,2组Hp感染情况比较,观察组总有效率93.33%;对照组总有效率90.00%,差异无统计学意义(P>0.05)。治疗结束4个月后,观察组复发率10.71%,对照组37.04%,2组比较,差异有统计学意义(P<0.05)。胃溃疡疗效观察组痊愈率46.67%,总有效率93.33%;对照组痊愈率33.33%,总有效率76.67%。2组比较,差异有统计学意义(P<0.05)。治疗结束4个月后溃疡复发情况进行比较,观察组溃疡痊愈14例中,复发1例,复发率7.14%;对照组痊愈10例中,复发4例,复发率40.00%。差异有统计学意义(P<0.05)。治疗后,2组环氧合酶2(COX-2)、中间核转录因子(NF-кBp65)和三叶因子(TFF3)免疫组化表达阳性率比较,差异均有统计学意义(P<0.05)。2组各项中医症状积分较治疗前均有下降,差异均有统计学意义(P<0.05)。治疗后,观察组胃脘疼痛、得食痛减、四肢乏力、肢冷畏寒、纳呆食少症状积分分别与对照组比较,差异均有统计学意义(P<0.05)。在研究过程中,2组间的安全性指标均未见明显异常,无不良反应,治疗前后心、肝、肾功能,血、小便、大便常规检查及心电图检查均无明显异常。结论:蒲翘异功汤联合常规含铋剂四联疗法治疗脾胃虚寒证Hp相关性胃溃疡,其Hp清除率与单纯含铋剂四联疗法治疗虽无明显变化,但能降低Hp感染的远期复发率,不同程度改善脾胃虚寒证Hp相关性胃溃疡患者的中医症状,提高胃溃疡治愈率,降低胃溃疡远期复发率,有效降低COX-2、NF-кBp65、TFF3肿瘤风险因子表达水平,且临床安全性高。     

护理指导功能锻炼结合针刺与中频对类风湿性关节炎疗效的影响

目的探讨护理指导功能锻炼结合针刺与电脑中频对类风湿性关节炎疗效的影响。方法将2011年3月至2013年5月76例我院住诊的类风湿性关节炎患者随机分为研究组和对照组,研究组40例采用护理指导功能锻炼结合针刺与电脑中频综合治疗,对照组36例采用针刺与电脑中频治疗。结果研究组和对照组的治愈率分别为47.50%和16.67%,临床总有效率分别为90.00%和72.22%,经Ridit分析发现,研究组疗效明显优于对照组(P<0.01)。结论护理指导功能锻炼可明显提高针刺与电脑中频治疗类风湿性关节炎的临床疗效,具有一定的临床价值,值得推广应用。     

甲状腺全切除术中间入路60例的临床效果分析

目的观察中间入路甲状腺全切除术的临床效果。方法回顾性分析2013年1月-2014年1月本院收治的120例甲状腺全切除术患者的临床资料,将患者随机分为观察组与对照组,各60例。观察组采用中间入路甲状腺全切除术,对照组采用传统甲状腺全切除术,对比两组的手术时间、术中出血量、术后甲状旁腺功能低下及喉返神经损伤发生率。结果观察组手术时间短于对照组,术中出血量少于对照组,术后甲状旁腺功能低下、喉返神经损伤发生率低于对照组,差异有统计学意义（P〈0.05）。两组均未出现永久性甲状旁腺功能低下与喉返神经损伤的病例。结论同传统甲状腺全切除术相比,中间入路甲状腺全切除术更加安全、可靠,值得临床推广应用。     

冠心病不同进展阶段差异表达基因筛选

目的基于转录组学分析不同进程冠心病患者差异表达基因图谱,探索与冠心病进展相关的特异性基因。方法经冠脉造影与血样质控,选择8例冠脉临界病变患者(临界组),并根据性别、年龄匹配冠脉造影正常者8例(对照组),急性心肌梗死患者8例(心梗组),通过Illumina平台的HiSeq高通量测序技术对不同组别患者外周血单核细胞基因组测序,筛选差异表达基因(differentially expressed genes,DEGs),并进行GO条目富集分析差异基因功能。结果临界组与对照组相比,有169个DEGs,110个上调和59个下调(基因集1);心梗组与临界组相比,有376个DEGs,246个上调和130个下调(基因集2)。最显著富集的GO条目包括炎症反应及mRNA稳定性调节、蛋白结合、细胞核浆及分泌颗粒等条目。基因集1、2交集基因包括DKK3、NR4A1、HIP1和PAX8-AS1。结论本研究初步获取了不同进程冠心病患者差异表达基因图谱,发现DKK3、NR4A1、HIP1和PAX8-AS1基因表达改变可能与冠心病进展相关。     

气管-主支气管分支型部分覆膜内支架治疗主支气管内膜结核瘢痕性狭窄随访研究

目的观察气管-主支气管分支型部分覆膜内支架植入治疗主支气管内膜结核瘢痕性狭窄后长期疗效和并发症。方法选择2003年4月至2007年1月郑州大学第一附属医院放射科17例有较完整的随访资料的主支气管内膜结核瘢痕性狭窄放置气管-主支气管分支型部分覆膜内支架患者,17例患者分别在支架取出后第1、6个月行视觉类比测试法(VAS)。其中14例分别于支架取出后第1、6个月门诊行胸部CT和支气管镜复查,电话随访与信访3例。结果17例均一次性植入支架成功并在25～90d内成功取出支架,随访6～50个月,1例患者在支架取出后7d发生再狭窄,经治疗后未再发生呼吸困难,其余患者均未再次出现呼吸困难。支架取出后第1、6个月,17例患者VAS均较术前有明显改善(P<0.05)。结论气管-主支气管分支型部分覆膜内支架植入治疗主支气管内膜结核瘢痕性狭窄,操作成功率高,远期疗效可靠。     

Bronchial anomalies in VACTERL association

VACTERL association is an acronym made of associated defects including vertebral anomalies, anal atresia, cardiac, tracheal-esophageal fistula, and renal/radial limb anomalies. Tracheal bronchus is a condition characterized by ectopic location of the right upper lobe bronchus at the mid to distal trachea. This condition is associated with congenital anomalies and has been reported in one previous case of VACTERL. We report another infant with VACTERL presenting with respiratory complications due to presence of tracheal bronchus. She also had a narrowed segment of her right main stem bronchus.     

胎盘生长因子在慢性哮喘大鼠肺组织中的表达

 目的 探讨胎盘生长因子(placenta growth factor,PlGF)在慢性哮喘大鼠肺组织中的表达。方法 45只雄性SD大鼠随机分为3组(每组15只):(1)正常大鼠组(A组):未行特殊干预处理;(2)卵清蛋白(ovalbumin,OVA)致敏及生理盐水吸入大鼠组(B组):应用OVA致敏生理盐水激发;(3)慢性哮喘大鼠组(C组):应用OVA致敏和反复激发制备大鼠慢性哮喘模型。各组大鼠于末次激发后24 h处死。ELISA法测定肺泡灌洗液(BALF)中PlGF水平;大鼠肺组织标本行HE染色、PAS染色及Masson三色染色,并行病理图像形态学测定和分析;免疫组化检测大鼠气道上皮细胞PlGF的表达。结果 慢性哮喘大鼠组(C组)气道上皮细胞PlGF的表达(PI为2.28±0.18)与正常大鼠组(A组)及OVA致敏生理盐水吸入大鼠组(B组),(PI分别为0.89±0.08、0.94±0.12)相比明显增多(A组与C组比较P<0.01,B组与C组比较P<0.01)。慢性哮喘大鼠组(C组)BALF中PlGF水平(18.87±4.53)ng/ml与正常大鼠组(A组)及OVA致敏生理盐水吸入大鼠组(B组),分别为(12.35±1.94)ng/ml、(13.14±2.52)ng/ml相比明显增高(P<0.05)。结论 胎盘生长因子(PlGF)在慢性哮喘大鼠肺组织中表达增多。