Evaluation of wound healing activity of root of Mimosa pudica

Ethno pharmacological relevance

Mimosa pudica, commonly known as touch-me-not, is used in folklore medicine in arresting bleeding and in skin diseases.

Aim

There was no scientific evidence justifying the use of Mimosa pudica, therefore the present study was aimed at evaluation of wound healing activity of the plant.

Materials and methods

In the present study the roots of Mimosa pudica were studied for wound healing activity by incorporating the methanolic and the total aqueous extract in simple ointment base B.P. in concentration of 0.5% (w/w), 1% (w/w) and 2% (w/w). Wound healing activity was studied in three types of model in rats viz. excision, incision and estimation of biochemical parameter. In case of the excision wound model wound contraction and period of epithelization was studied while in incision wound model was evaluated by determining tensile strength and hydroxyproline content in the scab.

Results

Treatment of wound with ointment containing 2% (w/w) the methanolic and 2% (w/w) the total aqueous extract exhibited significant (P < 0.001) wound healing activity. The methanolic and total aqueous extracts were analyzed for total phenols content equivalent to Gallic acid. The content of total phenols was 11% (w/w) and 17% (w/w) in methanolic and total aqueous extract respectively.

Conclusion

The methanolic extract exhibited good wound healing activity probably due to phenols constituents.     

Wound healing activity of Calotropis gigantea root bark in rats

Ethnopharmacological relevance

Calotropis gigantea R.Br. (Asclepiadaceae) is a perennial undershrub found chiefly in wastelands throughout India. It has been reported as a traditional folkloric medicine for a variety of alignments. The plant Calotropis gigantea is also used in some parts of India for wound healing in combination with other plants. However there are no scientific reports on wound healing activity of the plant Calotropis gigantea R.Br.

Aim of the study

To investigate the effects of Calotropis gigantea root bark on wound healing activity in rats by excision, incision and dead space wound healing models in rats.

Methodology

Wistar albino rats of either sex weighing between 180 and 200 g were topically treated with extract formulated in ointment by using simple ointment BP as base. 5% (w/w) ointment was applied once daily in excision wound model. Calotropis gigantea ethanolic extract was given orally at a dose of 100, 200, and 400 mg/kg in incision and dead space wound healing models. Rats of standard groups were treated with 5% Povidone iodine ointment topically. The percentage wound closure, epithelization time, hydroxyproline content and scar area on complete epithelization were measured.

Results

Topical application of Calotropis gigantea in excision wound model increased the percentage of wound contraction. Scar area and epithelization time were decreased. In incision wound and dead space wound breaking strength of wounds and hydroxyproline was increased.

Conclusion

Calotropis gigantea accelerated wound healing in rats and thus supports its traditional use.     

皮下置管负压引流防治肥胖患者腹部纵切口脂肪液化前瞻性研究

目的探讨术中预防性留置皮下负压引流管在防治肥胖患者腹部纵切口术后脂肪液化的必要性及临床价值。方法前瞻性选择2008年2月至2010年10月期间洛阳市第一人民医院普外科和妇产科的342例肥胖腹部纵行切口患者,按随机数字表法随机分为置管组及无管组,比较2组间切口甲级愈合率、脂肪液化发生率、切口并发症发生率、切口平均愈合时间、患者满意度、局部异物感及直接医疗成本。结果根据纳入和排除标准,共328例患者纳入本研究,其中置管组165例,无管组163例。2组患者在年龄、性别构成、脂肪厚度、手术时间、切口长度及BMI方面差异均无统计学意义(P>0.05)。置管组与无管组脂肪液化发生率分别为3.6%(6/165)和5.5%(9/163),P>0.05;异物感分别为50.3%(83/165)及3.7%(6/163),P<0.01;患者满意度分别为(6.8±1.7)分及(9.2±2.8)分(P<0.05)。甲级愈合率、切口并发症发生率、切口平均愈合时间及直接医疗成本2组间比较差异无统计学意义(P>0.05)。结论对肥胖患者腹部纵切口术中不必常规放置负压引流管预防切口脂肪液化。     

切开挂线配合留置梅花头导尿管引流术治疗高位肛周脓肿50例临床观察

为探讨高位肛周脓肿的更佳治疗方法,将100例高位肛周脓肿患者随机分为两组,采用切开挂线同时脓腔内留置梅花头导尿管引流术治疗50例为观察组,切开挂线引流术治疗50例为对照组。对术后疼痛、换药时疼痛、体温改善、疗程、遗留肛门锁眼畸形、肛门松弛、后遗肛瘘等情况进行观察。结果显示,观察组明显优于对照组（P〈0．05）。结果表明,采用切开挂线引流术的同时,脓腔内留置梅花头导尿管引流术治疗高位肛周脓肿,可提高一次性根治效果,该法既缩短了疗程,减少患者的痛苦,又保护了肛门括约肌的功能。     

切开挂线术治疗骨盆直肠间隙脓肿32例临床观察

目的探讨切开挂线术治疗骨盆直肠间隙脓肿的临床应用价值。方法自2008年1月至2009年3月对32例骨盆直肠间隙脓肿患者行切开挂线术。结果 32例均临床治愈,住院l5～26d（平均20d）,其中31例随访1年无复发,1例形成肛瘘,经再次手术治愈。结论切开挂线术治疗骨盆直肠间隙脓肿具有病程短,一次性根治率高等优点,且有效减轻或避免了肛门缺损。     

3D腹腔镜联合杂交技术在腹壁巨大切口疝中的应用体会

目的:探讨3D腹腔镜联合杂交技术治疗腹壁巨大切口疝的临床效果。方法:回顾分析2012年5月至2016年4月为25例腹壁巨大切口疝患者行3D腹腔镜联合杂交技术腹壁切口疝无张力修补术的临床资料,记录围手术期情况,总结其治疗效果。结果:25例患者均顺利完成手术,手术时间平均(111.7±11.3)min,切口长度平均(7.1±3.5)cm,术中出血量平均(55.1±7.7)ml,术后平均住院(6.7±1.6)d;4例患者术后使用止痛剂。术中肠道浆膜损伤1例,未影响补片修补;术后切口轻度感染1例,积极治疗后痊愈,未取出补片,无血清肿、肠瘘等其他并发症发生。术后随访24个月,随访率100%,无一例复发。结论:3D腹腔镜联合杂交技术治疗腹壁巨大切口疝是安全、有效、可行、实用的,治疗效果较好,结合了腹腔镜与开放手术的优点,术后患者康复快,美容效果好,值得临床推广应用。     

Transitioning to Single-Incision Laparoscopic Inguinal Herniorrhaphy

Background:

Laparoendoscopic single-site surgery (LESS) offers cosmetic benefits and may represent further progress towards reducing the invasiveness of surgical interventions. We report our initial experience with LESS totally extraperitoneal (TEP) inguinal herniorrhaphy.

Materials and Methods:

Beginning March 2009, we transitioned from a multiport laparoscopic TEP (MLH) technique to a single-incision TEP (SITE) technique. The first 52 consecutive patients who underwent SITE at our institution were compared with the preceding 52 MLH repairs.

Results:

Of the first 52 patients undergoing SITE, there were no conversions to either open or multiport surgery. The mean operative time for the SITE cases did not differ significantly from that of MLH. Complications were equivalent between the 2 groups and included postoperative seroma and urinary retention.

Conclusions:

Transitioning from MLH to SITE was readily accomplished without significantly altering operative time or morbidity.     

近端切开挂线远端旷置对口引流法治疗高位复杂性肛瘘的疗效观察

为探讨近端切开挂线远端旷置对口引流法治疗高位复杂性肛瘘的临床疗效,对64例高位复杂性肛瘘患者采用此法治疗。结果显示,64例患者全部治愈,未出现肛门部分失禁、肛门完全失禁、局部大瘢痕形成、肛门畸形等后遗症。创面愈合时间为15～3ld,平均20d。随访1年,均无复发。结果表明,近端切开挂线远端旷置对口引流法治疗高位复杂性肛瘘疗效肯定,术后后遗症少。     

乳腺良性肿瘤手术改良切口及其对乳房表面美观的影响

目的探讨乳腺良性肿瘤手术改良切口的方法及其对乳房表面美观的影响。方法选取2010年1月至2011年2月收治的乳腺良性肿瘤择期手术患者86例,设为对照组,于病灶处行开放性切口或放射状切口进行乳腺良性肿瘤切除手术：选取2011年3月至2012年6月收治的乳腺良性肿瘤择期手术患者93例,设为观察组,选用环乳晕缘弧形切口进行乳腺良性肿瘤切除手术。比较两组患者的围手术期情况,进行乳房表面美观评分（BSBs）,比较患者满意度,随访1年观察乳房良性肿瘤有无复发或新发。结果两组均顺利完成手术,按预期完整、彻底的切除了肿瘤,两组在手术切除肿瘤效果、术后并发症、复发（含新发）率等方面比较,差异均无统计学意义（P〉0．05）。观察组患者术后乳房形状、切口瘢痕、色素沉着、乳头乳晕感觉及BSBs,患者对术后乳房外观的满意程度均优于对照组,差异具有统计学意义（P〈0．05）。结论乳腺良性肿瘤手术改良切口能够完整、彻底切除肿瘤,且术后瘢痕不明显,保存了乳房较好的表面美观效果,术后乳房更美观,且未增加并发症以及术后复发率,更能满足年轻女性的审美要求,患者对手术的满意度高,值得临床广泛推广应用。     

泌尿外科感染与手术切口感染相关性分析及护理

目的 探讨泌尿外科感染与手术切口感染的临床相关性及护理特点,为临床治疗提供依据.方法 选取医院2009年2月-2012年2月收治的泌尿外科手术患者病历资料,对手术感染病例、切口、缝合情况及术前尿液培养分离、药敏试验结果进行分析.结果 128例泌尿外科手术患者15例发生术后手术部位感染,其中以金黄色葡萄球菌、表皮葡萄球菌为主；发生手术部位感染的病例皮下组织采样病原菌分离率高于未发生手术感染的患者；手术切口病原菌耐药率高于未发生感染的分离菌；切口感染的患者术前泌尿系统感染发生率高,手术切口感染患者中9例伴泌尿系感染,6例分离菌株为葡萄球菌属.结论 泌尿系感染是手术切口感染的重要因素,针对尿路感染患者进行护理是减少术后切口感染的重要措施.