类消胶囊治疗类风湿关节炎的临床观察与实验研究

目的 研究类消胶囊对类曲湿性关节炎的治疗作用。方法 对３０例类风湿关节炎患者服用类消胶囊的后进行血清ＮＰＴ－２Ｒ水平测定。３００你储血员作为对照组,测得ＮＰＴ≤１００ｍｇ／ｍｌ、Ｈ、２Ｒ≤２５０ｕ／ｍｌ。结果 服前药ＮＰＴ与ＩＬ－２Ｒ水平明显高于对照组（Ｐ〈０．００１）。服药后ＮＰＴ与ＩＬ－２Ｒ测定其平均值明显低于有取药前（Ｐ〈０．００１）。结论 类消胶囊可降低ＮＰＴ和ＩＬ－２Ｒ,而ＮＰＴ和ＩＬ－     

载脂蛋白A1及B100火箭电泳法测定

应用火箭电泳法测得65例健康人血清载脂蛋白 A1（APOA1）为1.149±0.138g/L（114.9±13.8mg/dl）,其中男性（39人）1.177±0.136g/L（117.7±13.6mg/dl）,女性（26人）1.123±0.139g/L（112.3±13.9mg/dl）;血清载脂蛋白 B100(APOB100)为0.898±0.158g/L（89.8±15.8mg/dl）,其     

Guidelines for the Validation and Use of Immunoassays for Determination of Introduced Proteins in Biotechnology Enhanced Crops and Derived Food Ingredients

Recent advancements in agricultural biotechnology have created a need for analytical techniques to determine introduced proteins in crops enhanced through modern biotechnology techniques. These proteins are expressed in plant tissues and may be present in food ingredients. Immunoassays are ideally suited for protein detection and may be used as both quantitative and threshold methods. Microplate ELISA and lateral flow devices are two of the most commonly used immunoassay formats for agricultural biotechnology applications. This paper provides general background information and a discussion of criteria for the validation and application of immunochemical methods to the analysis of proteins introduced into plants and food ingredients using biotechnology methods. It is the result of a collaborative effort of members of the Analytical Environmental Immunochemical Consortium. This collaborative effort represents the combined expertise of several organizations to reach consensus on establishing guidelines for the validation and use of immunoassays. Further, the paper offers developers and users a consistent approach to adopting the technology as well as aid in producing accurate and meaningful results.     

69例少精、无精症患者血清性激素水平分析

目的探讨少精、无精症患者血清性激素检测的临床应用价值。方法采用化学发光免疫分析法(CLIA)测定21例无精症、48例少精症(其中13例为重度少精症)患者与25例精液正常健康体检男性的血清性激素水平。结果无精症组及重度少精症组卵泡刺激素(FSH)、黄体生成素(LH)均显著高于正常精液组(P<0.01),且FSH升高幅度均大于LH。少精症组FSH、LH虽较正常精液组高,但无显著性意义(P>0.05)。与正常精液组比较,无精症组、少精症组、重度少精症组垂体催乳素(PRL)均呈显著性升高(P<0.01),睾酮(T)均呈显著性降低(P<0.01),而雌二醇(E2)、孕酮(P)均无显著性变化(P>0.05)。无精症组FSH、LH、PRL升高者分别占66.7%、61.9%和28.6%,T降低者为42.9%;重度少精症组FSH、LH、PRL升高者分别为53.8%、38.5%和30.8%,T降低者为38.5%。FSH、LH、T均呈降低者,无精症组14.3%,重度少精症组7.7%;FSH、LH升高而T在正常参考范围者,无精症组38.1%,重度少精症组23.1%;FSH、LH升高而T降低者,无精症组23.8%,重度少精症组15.4%;FSH、LH、T均在正常参考范围者,无精症组19.0%,重度少精症组23.1%。结论血清性激素水平检测应用于少精症(特别是重度少精症)、无精症患者的病因分析、制定治疗措施和预后判断具有重要的临床意义。     

Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA

Ciguatera fish poisoning (CFP) is a form of food poisoning caused by the ingestion of a variety of reef fish that have accumulated trace amounts of ciguatoxins produced by dinoflagellates of the genus Gambierdiscus through the food chain. CFP affects more than 50,000 people each year. The extremely low level of the causative neurotoxins, ciguatoxins, in fish has hampered the preparation of antibodies for detecting the toxins. In this paper, we describe a thiol strategy for synthesizing a keyhole limpet hemocyanin (KLH)-conjugate (20) of the ABCDE-ring fragment of the Pacific ciguatoxins, CTX1B (1) and 54-deoxyCTX1B (4). We succeeded in producing a monoclonal antibody (3G8) against the left wings of these ciguatoxins by immunizing mice with the hapten-KLH conjugate (20) as the synthetic antigen. The most promising mAb, 3G8, does not cross-react with other related marine toxins. Sandwich enzyme-linked immunosorbent assay (ELISA) utilizing 3G8 and the previously prepared monoclonal antibody (8H4) enabled us to detect 1 specifically at less than 0.28 ng/mL.     

流式细胞术不同设门方法对CD4+CD25+调节性T细胞的检测方法比较

目的 比较CD4+CD25hi、CD4+CD25+FoxP3+和CD4+CD25+CD127-/low 3种设门方法对CD4+CD25+调节性T细胞(Treg细胞)的界定效果.方法 完全随机法选择33例本院健康体检者,分别采用CD4+CD25hi、CD4+CD25+FoxP3+和CD4+CD25+CD127-/low设门的方法进行检测.结果 (1)CD4+CD25hi、CD4+CD25+FoxP3+设门方法对CD4+CD25+Treg细胞的检测结果差异无统计学意义[(6.85±2.72)%,(6.69±2.30)%,t=0.270,P=0.788],相关性检验显示,两者呈正相关(r=0.866,P＜0.001);(2)CD4+CD25+CD127-/low设门方法的细胞检测结果[(8.07±2.18)%]高于CD4+CD25hi和CD4+CD25+FoxP3+设门方法(t=2.055,P=0.043;t=2.325,P=0.022),相关性检验显示,均呈正相关(r=0.615,P＜0.001;r=0.683,P＜0.001);(3)CD4+CD25hi细胞群中FoxP3+细胞占(57.11±12.42)%,CD4+CD25hi细胞群中FoxP3+细胞占所有FoxP3+细胞的比例为(54.4±9.0)%.结论 CD4+CD25hi设门方法可用于人外周血CD4+CD25+Treg细胞的检测,CD4+CD25+CD127-/low设门方法可应用于CD4+CD25+Treg细胞的纯化.     

Interference in immunoassays by human IgM with specificity for the carbohydrate moiety of animal proteins

In allergen characterization common immunoassays such as ELISA and immunoblotting are often used to evaluate human IgE binding of sera from allergic individuals. In an attempt to find and characterize new cat allergens, heavily stained bands were found at 67 and 90 kDa on blotted cat dander extract. Further characterization confirmed these bands to be cat IgA and IgM heavy chains. Analyses in ELISA of purified cat immunoglobulins linked the human IgE binding to cat IgA and IgM and also revealed complete mutual cross-reactivity between the two isotypes. IgE reactivity was found to be directed to carbohydrates of the immunoglobulins and to IgM from 7 out of 9 animal species, but not to human immunoglobulins. Further investigations revealed that the reaction was not mediated by human IgE but by a factor in the serum cross-linking animal immunoglobulins and alkaline phosphatase. The factor was further studied and shown to be human IgM. This IgM fits into the heterophile antibody classification and is directed against carbohydrates on animal immunoglobulins and on calf intestine alkaline phosphatase.     

角膜上皮干细胞定位特征的免疫组织化学研究

利用单克隆抗体ＡＥ５与分化型角膜上皮细胞中角蛋白Ｋ３特异性结合,研究缺乏分化标志特征的角膜上皮干细胞定位特点,应用免疫组织化学方法显示Ｋ３阳性表达的区域分布于除角膜缘上皮基底部以外的所有角膜上皮细胞中,角膜上皮干细胞存在于角膜缘基底部ＡＥ５抗体反应阴性细胞中,即角膜干细胞位于角膜缘上皮层基底部。     

Rapid detection of the poly-γ-d-glutamic acid capsular antigen of Bacillus anthracis by latex agglutination

Latex agglutination has been used to detect capsular polysaccharides from a variety of bacteria in body fluids. A latex agglutination assay was constructed for detection of the poly-γ-d-glutamic acid (γDPGA) capsular polypeptide of Bacillus anthracis in serum from animal models of pulmonary anthrax. The assay was able to detect γDPGA in serum from infected animals at concentrations of 100 to 200 ng/mL.