术后自控镇痛对手术患者应激反应与细胞免疫功能的影响

目的:探讨术后自控镇痛对手术患者应激反应与细胞免疫功能的影响,以及术后自控镇痛保护免疫功能的可能作用机制.方法:将剖宫产待产妇80例随机分为镇痛组与对照组,每组40例,镇痛组予以术后自控镇痛,对照组不予以术后自控镇痛.结果:镇痛组患者取得满意的镇痛效果,术后6h、12h、24h、48h各观察时间点视觉模拟评分(VAS)...     

IL-17、MMP-3在类风湿关节炎患者滑膜液及血浆中的表达及意义

目的:分析类风湿关节炎(RA)患者滑膜液及血浆中白细胞介素-17(IL-17)、基质金属蛋白酶-3(MMP-3)的水平及其临床意义.方法:应用酶联免疫吸附法(ELISA)检测RA患者滑膜液及血浆中IL-17及MMP-3的水平,分析IL-17及MMP-3与临床指标的关系.结果:RA患者滑膜液IL-17及MMP-3水平明显...     

Th17细胞及其细胞因子在肺部感染中的作用

Th17细胞是CD4+T细胞的一个亚型,最近几年对其研究较多,它能分泌多种细胞因子,主要有IL-17A、IL-17F、IL-22、IL-26和IFN-γ.其中IL-17和IL-22在肺部抗感染方面发挥了重要作用.肺黏膜抗胞外菌特别是革兰氏阴性菌感染过程中,IL-17和IL-22一方面能促进抗微生物肽(防御素)的生成,另...     

围生期孕妇血清IL-6、TNF-α及外周血T淋巴细胞亚群变化的研究

目的:探讨围生期孕妇血清IL-6、TNF-α及外周血T淋巴细胞哑群变化规律.方法:选取本院100例产妇为研究对象,将其分别于分娩发动前、妊娠发动时及分娩后1、3 d的血清IL_6、TNF-α及外周血T淋巴细胞亚群水平进行统计及比较.结果:经研究发现,妊娠发动时血清IL-6、TNF-α高于分娩前及分娩后1、3 d,CD3+、CD4+、CD8+及CD4/CD8 水平均呈现先降后升的趋势,经比较,P均<0. 05,均有显著性差异.结论:围生期孕妇血清IL-6、TNF-α,及外周血T淋巴细胞亚群水平呈现一定的变化规律,值得进一步研究探讨.     

IL-6信号通路与肿瘤

IL-6(intedeukin-6)是迄今为止发现的功能最为广泛的细胞因子之一,参与调节免疫反应、血细胞的生成及多种细胞的增殖和分化.IL-6通过与其受体结合,继而活化胞内一系列信号蛋白分子,最终实现IL-6反应基因的表达.起初对IL-6的研究是将其作为一种炎性因子,后来又发现它与肿瘤的发生、发展及侵袭、转移密切相关.现对IL-6及其受体介导的信号通路及IL-6在肿瘤发生、发展中的作用及其机制作一简要综述.     

TLR2激动剂Pam3CK逆转IL-10转染小鼠髓样树突状细胞免疫功能的研究

目的:观察TLR2激活剂Pam3CK对IL-10转染小鼠髓样树突状细胞免疫功能影响。方法:转染IL-10至小鼠髓样树突状细胞(mDC),TLR2配体Pam3CK刺激48小时,利用流式细胞仪检测DC表面标志MHCⅡ、CD80、CD86及FasL等分子的表达;ELISA检测细胞产生IL-6、TNF-α。结果:IL-10抑制mDC表达CD80、CD86、MHCⅡ类分子,降低其分泌IL-6、TNF-α,促进其表达FasL,而TLR2激动剂刺激增加了IL-10转染DC表达MHC-Ⅱ类分子及CD80、CD86,促进其产生IL-6及TNF-α,抑制了FasL表达。结论:TLR2激动剂可逆转IL-10诱发的DC免疫应答低下。     

脑胶质瘤的MRI影像表现及其与IL-17、IL-24、VEGF、GFAP的相关性研究

目的探讨脑胶质瘤的MRI影像表现及其与脑胶质瘤组织中白细胞介素-17(IL-17)、白细胞介素-24(IL-24)、血管内皮生长因子(VEGF)、胶质纤维酸性蛋白(GFAP)阳性表达的相关性。方法回顾性分析我院神经外科68例经手术病理证实为脑胶质瘤患者的临床资料并将其纳入研究组,以79例因脑出血行开颅手术患者为对照组。分析研究组患者的MRI影像表现并通过HE染色、免疫组化SP法检测2组患者中IL-17、IL-24、VEGF、GFAP表达的差异性,应用Speraman等级相关分析IL-17、IL-24、VEGF、GFAP表达与MRI表现的关系。结果脑胶质瘤位于大脑半球者65例(95.59%),脑胶质瘤位于小脑半球者3例(4.41%)。66例(97.06%)表现为T1WI低、T2WI混杂信号伴周围中—重度水肿,2例(2.94%)表现为T1WI稍高、T2WI混杂信号;肿瘤内出血31例(45.58%),无出血37例(54.42%);2例(2.94%)表现为轻度水肿,2例(2.94%)表现为中度水肿,64例(94.12%)表现为重度水肿;44例(64.71%)脑胶质瘤内见流空血管影,脑胶质瘤生长速度快,瘤体均较大,最大截面为0.7 cm×1.2 cm~10.3 cm×9.6 cm,形态不规则。DWI呈弥散受限改变66例(97.06%),无弥散受限2例(2.94%);增强扫描后均匀强化59例(86.76%),不均匀9例(13.24%);59例(86.76%)呈花环状或花瓣状明显强化,环壁部分呈栅栏状,9例(13.24%)呈轻度结节状强化。IL-17、VEGF在对照组中不表达,研究组IL-17、VEGF表达阳性率高于对照组,IL-24、GFAP表达阳性率低于对照组,差异均有统计学意义(P<0.05)。Speraman等级相关分析显示,IL-24、GFAP表达阳性率随脑胶质瘤分级升高呈下降趋势,呈显著负相关(r=-0.711、-0.577,P<0.05);IL-17、VEGF表达阳性率随脑胶质瘤分级升高呈上升趋势,以Ⅳ型胶质瘤表达最高,呈显著正相关(r=0.670、0.614,P<0.05)。结论脑胶质瘤MRI表现具有瘤体大、累及脑叶多、形态不规则,信号不均匀,水肿明显的特点,肿瘤组织中IL-17、IL-24、VEGF、GFAP的阳性表达能够反映脑胶质瘤分级程度。     

小剂量顺铂通过下调Th17细胞频率缓解小鼠自身免疫性肝炎

目的 探索小剂量顺铂对小鼠自身免疫性肝炎(AIH)Th17细胞的影响及相关机制.方法 将24只小鼠随机分为正常组(normal group)、AIH+顺铂组(AIH+Cisplatin group)和AIH+生理盐水组(AIH+NS group).后2组利用S100/FCA建立小鼠AIH模型后,分别腹腔注射0.2 ml...     

IL-10 promoter genetic polymorphisms and risk of Kawasaki disease in Taiwan

Kawasaki disease (KD) is the most common cause of pediatric acquired heart disease. KD patients have spontaneously high plasma/serum levels of IL-10 during the acute phase. Therefore, two independent studies were carried out to investigate the association between genetic variants in IL-10 promoter (-1082, -819, and -592) and risk of KD. A total of 134 trios were included for the family-based association study. A significantly preferential transmission of the C allele at loci -819 T > C and -592 A > C for KD cases was observed (P permutation = 0.029 and P permutation = 0.034, respectively). There was a significant increase in the transmission of haplotype CC (p = 0.016) at the above two loci (OR, 1.632; 95% CI, 1.090-2.443; P permutation = 0.019). We also carried out a follow-up case-control study that included 146 KD cases and 315 unrelated healthy children. The haplotype CC (-819, -592) showed an increased risk of KD (but statistically non-significant; OR, 1.332; 95% CI, 0.987-1.797; p = 0.061). In diplotype analysis, a trend was found between number of CC haplotype and risk of KD (but non-significant, p =0.061). In conclusion, CC genotype and CC/CC diplotype at IL-10-819T > C and -592A > C were significantly associated with risk of KD in case-parent trio study, which were replicated partially in our follow-up case-control study.     

Effect of multiple activation stimuli on the generation of Th1-polarizing dendritic cells

It was originally reported that only a small fraction of total matured dendritic cells (DCs) produced interleukin (IL)-12, but it has never been determined whether different combinations of activating signals now shown to maximize secreted IL-12 do so through increasing output by the same IL-12 producers, or by recruiting additional cytokine-secreting cells. We therefore tested all combinations of bacterial lipopolysaccharide (LPS) (TLR4 ligand), R848 (TLR8 ligand), interferon (IFN)-γ, and CD40L for activating human monocyte-derived dendritic cells (DC), and determined by intracellular flow cytometry that enhanced IL-12 secretion was accomplished in large part by markedly increasing the proportion of cells producing IL-12, with the triple and quadruple combinations recruiting the most DC. This optimization requirement for multiple signals was not reflected in differential Toll-like receptor (TLR) expression by the cells. Interestingly, DCs activated with single TLR ligands plus IFN-γ were capable of responding with a second burst of IL-12 upon later CD40L stimulation, whereas DCs activated with R848 plus LPS were not, despite the trend of the latter for superior polarization of naive T cells toward IFN-γ-secreting Th1. These results have implications for the biology of IL-12-secreting DCs and choice of activation regimen for prospective use in DC-based immunotherapy.