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71.
Michael C. Lee Fei Dong Andrew J. Stephenson J. Stephen Jones Cristina Magi-Galluzzi Eric A. Klein 《European urology》2010
Background
Few reports attempt to validate the role of Epstein criteria in selecting patients for an active surveillance protocol.Objective
To determine the performance of the Epstein biopsy criteria for predicting pathologic end points and biochemical relapse-free survival (bRFS) in men with early stage prostate cancer (PCa) treated by radical prostatectomy (RP).Design, setting, and participants
Between October 1999 and January 2007, 746 consecutive patients were biopsied, and then underwent RP at our tertiary care institution. Two hundred sixty-eight patients met the entry criteria of Gleason 6 disease only on initial biopsy with complete pathologic information.Measurements
Primary end point was insignificant disease. Insignificant disease was defined using a classical (organ-confined, Gleason score <6, and tumor volume <0.5 cm3) and more liberal (organ-confined, Gleason <6 tumor of any volume) formulation. Secondary end points included organ-confined disease and bRFS.Results and limitations
One hundred thirty-six men (51%) met the Epstein biopsy criteria, and 167 (62%) had organ-confined cancer. Insignificant disease by the classical and liberal definitions was present in 68 (25%) and 92 (34%) patients, respectively. Cases meeting Epstein biopsy criteria were more likely to have insignificant disease by either definition (p < 0.001) and more likely to have organ-confined tumors (p < 0.001). Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) varied widely among the end points, with sensitivity (74%) and NPV (86%) best for the classical definition of insignificant disease and specificity (74%) and PPV (92%) best for organ-confined disease. The estimated 5-yr bRFS was 100% for those meeting Epstein biopsy criteria compared to 83% for those not meeting these criteria.Conclusions
The Epstein biopsy criteria predict for a high likelihood of organ-confined disease and the absence of biochemical failure up to 5 yr after RP. These criteria are insufficiently robust to predict the presence of biologically insignificant disease. 相似文献72.
鼻咽癌患者鼻咽组织和血清中EBV检测 总被引:2,自引:0,他引:2
目的:比较血清爱泼斯坦-巴尔病毒(EBV)抗体滴定度测定与癌组织EBV基因组检测对鼻咽癌(NPC)的诊断价值。方法:146例患者采用双盲法测定血清EBV-VCA-IgA,EBV-EA-IgA和活检组织EBV-DNA(PCR)。全组病例按活检病理检查结果分析:NPC组,非NPC组(对照组)。结果:NPC组中EBV-DNA(PCR),EBV-VCA-IgA和EBV-EA-IgA的阳性率分别为90.8% 相似文献
73.
bcl-2、P 5 3基因及Epstein-Barr病毒在鼻咽癌中的表达 总被引:1,自引:0,他引:1
目的探讨鼻咽癌组织中p53、bcl-2、EBV表达情况及与主要临床指标间关系.方法免疫组化检测p53、bcl-2蛋白,多聚酶链反应(PCR)检测EBV.结果慢性炎性组织中p53、bcl-2及EBV阳性率分别为7.7%、23.1%和7.7%,明显低于鼻咽癌组织中的阳性率(分别为75.9%、88.9%和87.0%)(P<0.01).三者在鼻咽癌组织中共同阳性率为72.2%,p53、bcl-2明显正相关(r=0.78,P<0.01).鼻咽癌伴颈淋巴结转移组p53阳性率(90.0%)明显高于无颈淋巴结转移组(58.3%)(P<0.01),临床Ⅱ、Ⅲ和Ⅳ期p53阳性率(分别为92.9%、78.9%和100%)均明显高于临床I期(38.5%)(P<0.01),且表达强度有增加的趋势.结论p53、bcl-2及EBV可作为鼻咽癌早期诊断的辅助指标之一,p53阳性的鼻咽癌患者较易发生颈淋巴结转移,随着鼻咽癌病程的发展,p53表达率及表达强度均增加,三者在鼻咽癌发生发展中起着重要作用. 相似文献
74.
Breast milk samples from 8 postpartum mothers were collected and incubated with Epstein-Barr virus, immortalizing their B-lymphocytes and giving rise to lymphoblastoid cell lines. Three samples showed no growing lymphocytes and five samples gave rise to transformed cell lines. IgA positive cells were selected from one such cell line by rosetting with anti-IgA-coated sheep erythrocytes. The emerging cell line was similarly reselected giving rise to an IgA-surface positive lymphoblastoid cell line. Monoclonal IgA-producing cell lines were obtained by cloning in the soft agar method. IgA is continuously secreted by the cell lines which have been growing in vitro for more than 24 mth. 相似文献
75.
目的探讨荧光定量PCR检测EB病毒(EBV)对诊断和治疗EBV感染的临床意义。方法应用荧光定量聚合酶链反应病毒核酸扩增方法,对儿科2010—06~2012—06以发热为主诉的患儿205例,检测外周血EB病毒DNA栽量,结合常规外周血及异型淋巴细胞检查,对照首发临床表现,分析荧光定量PCR检测发热患儿感染EBV的临床价值。结果205例发热患儿中,外周血EBV—DNA阳性42例,阳性率20.5%。荧光定量PCR检测EB病毒能早期反映EBV感染。结论荧先定量PCR检测EBv—DNA有助于早期诊断EBV感染,能减少诊疗的盲目性,对临床有一定指导意义。 相似文献
76.
Lymphocytic interstitial pneumonitis (LIP) in children has been most commonly associated with human immunodeficiency virus (HIV) infection. Epstein-Barr virus (EBV) associated LIP without HIV infection has been reported only in adults. EBV associated LIP has been reported in children, but only with concurrent HIV infection. We report a case of EBV associated, HIV negative LIP in a child. 相似文献
77.
Song KS Sung HJ 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2006,10(1):87-89
We report here the case of a patient suffering from hemophagocytic syndrome (HPS) associated with bile ductopenia. A 24-year old man was admitted after suffering fever, sore throat and general malaise for 7 days and jaundice for 2 days. Clinical studies showed hepatic dysfunction with hyperbilirubinemia. Epstein-Barr viral DNA from two bone marrow samples was detected. Bone marrow aspiration disclosed findings of HPS. Liver biopsy showed centrilobular cholestasis with lack of interlobular bile duct. Repeated therapeutic plasma exchange was effective for decreasing serum bilirubin and interleukin-6 levels. The patient received liver transplantation, however, he finally died of alveolar hemorrhage resulting from disseminated intravascular coagulation and acute rejection. 相似文献
78.
Alizée Jandin Cécile Pochon Arnaud Campidelli Maud D'Aveni Céline Kicki Anne-Béatrice Notarantonio Gabrielle Roth Guepin Tshinguta Anita Mbuyi Pierre Feugier Pascal Chastagner Cyril Schweitzer Marcelo de Carvalho Bittencourt Marie Thérèse Rubio Simona Pagliuca 《British journal of haematology》2023,202(1):122-134
An efficient immunological reconstitution construes the pillar for the success of allogeneic haematopoietic cell transplantation (HCT) in haematological disorders. Factors influencing post-transplant immune recovery have been largely investigated across multiple cohorts issuing heterogeneous results. Differences in outcomes in adult and paediatric populations suggest an age-related contribution to post-transplant immune reconstitution; however, it is unclear how recipient and donor age may affect the dynamics of single immune cells. Here, we retrospectively collected and analysed immunological data of 174 patients (58 children and 116 adults) consecutively transplanted for haematological disorders in our centre. We show that trajectories of specific immune cells were strictly dependent on recipient age and pretransplant virus exposure, with the strongest effect seen on T CD4+ and B-cell counterparts, while donor age and transplant platforms had a minimal impact. This mirrored different kinetics of immune reconstitution in adult and paediatric patients, with major divergences in immune cell composition in late post-transplant phases, featuring better survival, relapse-free survival and cumulative incidence of pathogen-specific infections in younger patients. Altogether, these findings underpin the importance of recipient age on post-transplant immune cell recovery and define the basic dynamics of the immune reconstitution in paediatric and adult populations as a benchmark for future studies. 相似文献
79.
Emilie Malézieux Lucy Meunier Benjamin Rivière Dominique Larrey 《Clinics and research in hepatology and gastroenterology》2021,45(5):101599
Thiazide diuretics are prescribed daily and rarely hepatotoxic. We report the case of 86-year-old woman who was admitted in hospital for jaundice after taking hydrochlorothiazide. All differential diagnoses have been eliminated. The liver biopsy was compatible with drug-induced hepatitis. Clinical and biological manifestations improved after discontinuation of the treatment. The reported case is compared to three other cases in the literature. 相似文献
80.
《Expert opinion on biological therapy》2013,13(11):1193-1205
Epstein–Barr virus (EBV), discovered > 40 years ago from a Burkitt’s lymphoma biopsy, was the first virus to be directly associated with human cancer. EBV has two distinct life cycles in the human host; a lytic form of infection that produces new infectious virions, and a latent form of infection that allows the virus to persist in a dormant state for the lifetime of the host. EBV has evolved a life cycle that mimics the natural differentiation pathway of antigen-activated B cells, giving the virus access to its site of latent infection, the resting memory B cell. By steering infected cells through the various stages of lymphocyte differentiation, EBV is able to enter a cell type suitable for long-term latent persistence and periodic reactivation. However, its presence in various stages of B-cell development, and its ability to infect certain epithelial cells, can have pathogenic consequences, and can contribute to the development of a diverse group of lymphomas and carcinomas. The presence of EBV in the tumour cells of EBV-associated cancers might provide a basis for specific therapy. This article focuses on the contributions that the virus may play in different types of human cancer, particularly Burkitt’s lymphoma, Hodgkin’s lymphoma, lymphomas and lymphoproliferative diseases in the immunocompromised, and nasopharyngeal and gastric carcinoma. 相似文献