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11.
目的 :探讨大剂量阿糖胞苷 ( HDAC)治疗儿童急性白血病的效果和不良反应。 方法 :应用 HDAC治疗 3例急性髓细胞白血病 (每次 2 .0 g/m2 ,每 12 h1次 ,共 6次为一个疗程 )和 4例高危型急性淋巴细胞白血病 (每次1.0 g/m2 ,每 12 h1次 ,共 8次为一个疗程 )共 16个疗程 ,九个疗程在 HDAC结束后使用惠尔血 ( 2~ 3μg/kg)皮下注射 ,连续 10~ 14天。 结果 :6例按计划完成 HDAC治疗 ,并继续用常规方案治疗者 ,在 2 0~ 42个月的随访期内无病生存 ,1例 AL L- L3型在一个疗程 HDAC后出现中枢神经系统白血病复发 ,骨髓仍缓解 ,7个月后放弃治疗 .骨髓严重抑制和感染是最主要的不良反应 ,加用惠尔血可使粒细胞缺乏的持续时间缩短 ,感染发生率降低。 结论 :以 HDAC为主的联合化疗方案可安全地用于儿童急性白血病的强化治疗 ,对降低复发、提高无病生存率有积极意义 相似文献
12.
腺病毒介导的CD基因在人胰腺癌细胞中的靶向性表达 总被引:1,自引:1,他引:0
【目的】探讨腺病毒介导的大肠杆菌胞嘧啶脱氨酶 (E .coliCD)基因在胰腺癌细胞中靶向性表达的可能性及其作用。【方法】将含癌胚抗原 (carcinoembryonicantigenCEA)启动子的重组腺病毒感染人胰腺癌SW1990细胞 (CEA )、Capan 2细胞 (CEA-)和人宫颈癌Hela细胞 ,观察CD基因在 3种细胞中的表达及细胞对 5 FC的敏感性差异。【结果】腺病毒介导CD基因仅在SW1990细胞中呈专一性表达 ,转导CD基因的SW1990细胞对 5 FC的敏感性明显提高。【结论】含CEA启动子的CD基因疗法可能是胰腺癌靶向性基因治疗的可行途径。 相似文献
13.
14.
Nader Gordjani Rainer Burghard Dirk Müller Helga Mathäi Gunther Mergehenn Jekabs U. Leititis Matthias Brandis 《Pediatric nephrology (Berlin, Germany)》1995,9(4):419-422
The potential tubulotoxicity of tobramycin and cefotaxim were assessed in neonates by measuring the urinary level of adenosine deaminase binding protein (ABP) and urinary 1-microglobulin and 2-microglobulin. In a prospective study, 33 neonates who received tobramycin and cefotaxim for suspected neonatal sepsis were compared with 48 untreated newborns during the first 10 days of life. The urinary concentrations of ABP and its excretion rates, corrected for body weight and body surface area, were significantly increased from the 1st day of treatment. Urinary 1-microglobulin and 2-microglobulin were not elevated under tobramycin and cefotaxim during the first 2 days of treatment. We conclude that ABP may be a sensitive marker for the detection of proximal renal tubular injury during tobramycin and cefotaxim treatments of neonates. The increase in urinary ABP which occurs before an elevation of urinary 1-microglobulin and 2-microglobulin may reflect earlier structural than functional alterations. However, since none of the treated infants had signs of electrolyte disorders or glomerular dysfunction, the clinical relevance of ABP measurement should be reevaluated. 相似文献
15.
Dipeptidyl peptidase IV (DPPIV) and adenosine deaminase (ADA), two T cell associated enzymes, are known to have a possible interaction and play essential roles in immune system functioning. On the other hand, depression has been shown to be accompanied with some immune-inflammatory alterations. In this regard, in order to make a contribution to the understanding of the ongoing immune disturbances in depression, serum DPPIV and ADA activities were determined in minor and major depressives and compared with healthy controls. Both enzyme activities were found to be decreased in major depressives compared to controls while only DPPIV activity was significantly lower in major depressives than the minor depressives. There were significant inverse relationships between enzyme activities and the severity of depression. Moreover, a positive intracorrelation was found between decreased DPPIV and ADA levels. The correlated decrease in DPPIV and ADA, might be a further support for their possible association. Results also suggest that decreased enzyme activities might reflect the impaired immune state in depression while major depressed patients might have a greater tendency to immune dysfunction than the minor depressed ones. 相似文献
16.
目的 了解自杀基因胞嘧啶脱氨酶基因 (cytosinedeaminase ,CD)及其前药 5 氟胞嘧啶 (5 fluorucytosine,5 FC)和bcl Xs基因转移联合作用对卵巢癌细胞株生长的影响。方法 以复制缺陷型腺病毒为载体将CD基因和bcl Xs基因体外转染大鼠卵巢癌细胞株NUTU 19细胞 ,加入含 5 FC的培养基。MTT法检测培养细胞吸光度值 ,计算细胞存活率。结果 单一bcl Xs基因转移及单一CD /5 FC系统作用对NUTU 19细胞的生长抑制作用均随病毒滴度的增加而增大 ;将两者联合作用于NU TU 19细胞 ,其生长抑制率比两者单独使用时的生长抑制率之和更高 ,表现为协同效应 (P <0 .0 0 0 1)。结论 CD /5 FC系统与bcl Xs基因转移联合使用对NUTU 19细胞的生长抑制具有协同作用。 相似文献
17.
In order to determine whether the growth of ras oncogene-transformed cells and nontransformed cells was inhibited differently by the chemotherapuetic drug cytosine arabinoside
(Ara-C) their growth was analyzed by a novel colony-based assay that is sensitive and appropriate for heterogeneous cell populations.
Colonies of nontransformed NIH3T3 cells, or ras onco- gene-transformed NIH(ras) cells, were grown in the absence of drug and then divided into subclones. Subclones were allowed to continue to grow in
the absence or presence of drug. Growth inhibition was determined by comparing the growth of drug-treated subclones with the
growth of related untreated subclones. Colonies of nontransformed cells grown in the absence of the drug displayed a large
variation in growth, and when grown in the presence of the drug displayed a large variation in growth inhibition. Colonies
of transformed cells also displayed a large variation in the absence and presence of the drug. For each cell line, related
subclones were more similar to each other than to unrelated subclones, implying inheritance of growth rates and drug response.
For NIH3T3 cells, the growth of subclones in the presence of drug was highly correlated with the growth of related subclones
in the absence of drug. However, for NIH3T3(ras) cells the growth of subclones in the presence of drug was not correlated with the growth of related subclones in the absence
of drug. Therefore, ras oncogene-transformed and nontransformed cell populations differ in their response to Ara-C.
Received: 3 November 1995/Accepted: 30 July 1996 相似文献
18.
胞嘧啶脱氨酸基因治疗人胰腺癌的实验研究 总被引:6,自引:2,他引:4
探讨腺病毒介导的大肠杆菌胞嘧啶脱氨酶基因用于人胰腺癌基因疗法的可行性。方法将含癌胚抗原启动子的重组腺病毒感染人胰腺癌SW1990细胞和Capan-2细胞以及人宫颈癌Hela细胞,用逆转录聚合酶链反应(RT-PCR)和Westernblot检测CD基因在细胞中的表达,以MTT法比较细胞对5-氟胞嘧(5-FC)的敏感性差异。建立胰腺癌裸鼠皮下移植瘤模型,观察CD基因的原位治疗效应及安全性。结果腺病毒介 相似文献
19.
腺病毒介导的大肠杆菌CD自杀基因体内外对肿瘤杀伤作用的研究 总被引:1,自引:0,他引:1
编委会 Editorial Board名誉主编 Honorary Editor-in-chief吴孟超 Wu Meng chao(Second Military Medical University,Shanghai 200433学术顾问 Academic Advisers巴德年 Ba Denian(Chinese Academy of Medical Sciences,Beijing 100730)刘新垣 Liu Xinyuan(Shanghai Institute of Biochemistry,Chinese Academy of Sciences,Shanghai 200031)吴旻 WuMin(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)汤钊猷 Tang Zhaoyou(Shanghai Medical University,Shanghai 200032)主编 Editor-in-chief张友会 Zhang Youhui(Cancer Institute,Chinese Academy of Medical Sciences,Beijing,100021)副主编 Associate Editor-in-chief崔正言 Cul Zhenyan(Department of Immunology,Shandong Academy of Medical Sciences,Jinan 250001)钱振超 Qian Zhenchao(Department of Patho-physiology,Dalian Medical University,Dalian 116027)何球藻 He Qiuzao(Department of Immunology,Shanghai Medical University,Shanghai 200032)董志伟 Dong Zhiwei(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)常务副主编 Managing Editor-in-chief 相似文献
20.
目的探讨肺结核患者外周血淋巴细胞内腺苷脱氨酶(ADA)活性和全血淋巴细胞绝对值的相关性。方法用全自动生化分析仪及全血细胞计数仪分别测定肺结核患者外周血的ADA活性和淋巴细胞绝对值、比值。结果肺结核活动期组与非活动期组外周血ADA活性均显著高于正常人组(P<0.05),肺结核活动期组与非活动期组患者外周血ADA活性与淋巴细胞绝对值的相关系数分别为0.191和0.154,P>0.05。结论肺结核患者淋巴细胞内ADA活性升高,淋巴细胞绝对值及比值减低,肺结核患者淋巴细胞内ADA活性升高与淋巴细胞绝对值无明显相关。 相似文献