荧光原位杂交法检测成熟B淋巴肿瘤细胞c-myc断裂

目的建立荧光原位杂交(FISH)方法检测8号染色体c-myc基因断裂,为临床筛选Burkitt’s淋巴瘤和成熟B型淋巴细胞白血病(B-ALL)提供方法。方法11例临床标本,FISH检测5例阴性,6例阳性。结果期待阴性的5例标本全部阴性,期待阳性的6例标本4例呈现阳性。结论用FISH法检测Burkitt’s淋巴瘤/B-ALL相关的myc基因断裂可行并基本可靠。     

Clinical Implication of Toll-Like Receptors (TLR2 and TLR4) Polymorphisms in Adult Patients with Acute B-cell Lymphoblastic Leukemia

Backgrounds: Neutropenia after intensive chemotherapy of acute lymphoblastic leukemia (ALL) could lead to infectious complications that affect outcome of acute leukemia patients. Many single-nucleotide polymorphisms (SNPs) of Toll-like receptors (TLRs) can affect the genetic susceptibility to infections. We investigated the impact of different SNPs on the incidence of developing sepsis and pneumonia in patients with newly diagnosed B-ALL following induction chemotherapy. Subjects and methods: We analyzed three SNPs in the TLR2 (Arg753Gln) and TLR4 (Asp299Gly& Thr399Ile) genes using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) in a case control study of 40 precursor B-ALL patients and 50 control subjects. The risk of developing sepsis and pneumonia were assessed by multiple logistic regression analyses. Results: The presence of the TLR-2 AG polymorphism was significantly associated with pneumonia in B-ALL patients. Furthermore, TLR4 Thr399Ile AG was a risk factor for sepsis in B-ALL patients. Moreover; Significant association between TLR-2 AA, TLR-4 CC and TL-4 AA genotypes and longer OS were detected in studied B-ALL patients. Conclusion: We concluded that TLR-4 (AG and CT) genotypes are associated with high susceptibility to sepsis and pneumonia respectively; while, TLR-2, TLR-4 AA and TLR-4 CC genotypes  could predict good B-ALL patients outcome.     

Dual targeting of CD19 and CD22 against B-ALL using a novel high-sensitivity aCD22 CAR

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