Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation,Immune Modulation and Multi Differentiation

Adipose tissue stem cells (ADSCs) would be an attractive autologous cell source. However, ADSCs require invasive procedures, and has potential complications. Recently, urine stem cells (USCs) have been proposed as an alternative stem cell source. In this study, we compared USCs and ADSCs collected from the same patients on stem cell characteristics and capacity to differentiate into various cell lineages to provide a useful guideline for selecting the appropriate type of cell source for use in clinical application. The urine samples were collected via urethral catheterization, and adipose tissue was obtained from subcutaneous fat tissue during elective laparoscopic kidney surgery from the same patient (n = 10). Both cells were plated for primary culture. Cell proliferation, colony formation, cell surface markers, immune modulation, chromosome stability and multi-lineage differentiation were analyzed for each USCs and ADSCs at cell passage 3, 5, and 7. USCs showed high cell proliferation rate, enhanced colony forming ability, strong positive for stem cell markers expression, high efficiency for inhibition of immune cell activation compared to ADSCs at cell passage 3, 5, and 7. In chromosome stability analysis, both cells showed normal karyotype through all passages. In analysis of multi-lineage capability, USCs showed higher myogenic, neurogenic, and endogenic differentiation rate, and lower osteogenic, adipogenic, and chondrogenic differentiation rate compared to ADSCs. Therefore, we expect that USC can be an alternative autologous stem cell source for muscle, neuron and endothelial tissue reconstruction instead of ADSCs.     

脂肪干细胞在皮下软组织充填中的研究进展

脂肪干细胞(Adipose-Derived Stem CeLIs ADSCs)具有一般干细胞的特点,即多向分化潜能和稳定的体外多代增殖能力,其免疫相容性好,来源丰富,易于获得,可反复取材,无伦理学问题,作为组织工程的种子细胞有着非常重要的实际研究和应用价值.本文着重对脂肪干细胞在生物学特性、体外成脂诱导分化,以及与之相配的生物学载体支架方面的最新进展进行阐述.     

脂肪干细胞对TNF-α诱导的牙周膜干细胞炎症因子表达的影响

目的 观察脂肪干细胞对肿瘤坏死因子-α(TNF-α)诱导的牙周膜干细胞炎症因子表达的影响,以期为脂肪干细胞应用于牙周炎治疗提供理论指导.方法 培养、鉴定脂肪干细胞和牙周膜干细胞.采用Transwell细胞共培养系统,上、下室均接种第3代细胞.实验分3组:A组:牙周膜干细胞对照组,上、下室均接种牙周膜干细胞;B组:肿瘤坏死因子-α诱导组,上、下室均接种牙周膜干细胞,下室加入10ng/mL肿瘤坏死因子-α;C组:肿瘤坏死因子-α联合脂肪干细胞干预组,上室脂肪干细胞,下室牙周膜干细胞,下室加入10 ng/mL肿瘤坏死因子-α.逆转录聚合酶链反应检测牙周膜干细胞白介素-6、白介素-8和肿瘤坏死因子-α的mRNA表达.结果 B组较A组白介素-6、白介素-8和肿瘤坏死因子-α的表达均增加,且均在2h时达到峰值(P〈0.05).2h时C组较B组白介素-6、白介素-8和肿瘤坏死因子-α的表达明显下降(P〈0.05).结论 脂肪干细胞能抑制TNF-α诱导的牙周膜干细胞的炎症反应.     

Klotho基因转染促进脂肪间充质干细胞增殖的实验研究

目的探讨klotho基因转染对脂肪间充质干细胞(ADSCs)增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern blot分析显示ADSCs转染klotho基因组细胞周期蛋白D1增高。结论转染klotho基因有促进ADSCs增殖的作用。     

三维支架材料与脂肪干细胞的生物相容性

背景：构建组织工程化气管需要适合的三维支架。 目的：观察脂肪干细胞与聚乳酸-乙醇酸共聚物及聚三亚甲基碳酸酯共聚物支架的生物相容性。 方法：采用组织块法原代分离培养SD大鼠脂肪干细胞,行流式细胞术及多向分化能力鉴定。将脂肪干细胞分别种植于聚乳酸-乙醇酸共聚物和聚乳酸-乙醇酸-三亚甲基碳酸酯共聚物支架中,扫描电镜观察细胞与支架的生物相容性。 结果与结论：脂肪干细胞种植于两种支架材料后生长速度快,扫描电镜观察可见脂肪干细胞呈球型,并伸展形成伪足,贴附于支架材料,细胞间相互连接成团。说明聚乳酸-乙醇酸共聚物与聚三亚甲基碳酸酯共聚物支架均具有良好的生物相容性,无细胞毒性,其多孔的三维立体状结构适合脂肪干细胞黏附生长。     

人大网膜间充质干细胞的体外培养、鉴定及诱导化分方法

目的建立人大网膜脂肪问充质干细胞（adiposederivedIIlesechymalSteITIcells,ADSCs）的原代培养及诱导分化为脂肪细胞的方法．方法术中取人大网膜脂肪组织,采用胶原酶消化法原代培养,经流式细胞仪鉴定细胞CD44、CD34、CD90和CD45分子、MTTr法测定细胞生长曲线,取P3细胞以3-异丁基-1-甲基黄嘌呤、胰岛素、地塞米松及吲哚美辛诱导该细胞向脂肪细胞分化,油红O脂肪染色法进行脂肪细胞鉴定．结果培养卅的细胞形态均-呈梭形或漩涡状生长,经流式细胞仪鉴定为ADSCs,细胞生长曲线表明P3细胞增值旺盛,用分化培养基诱导后,细胞形态由梭形变为圆形,细胞体积增大,胞浆内聚集脂肪颗粒,油红0染色鉴定为成熟的脂肪细胞．结论该方法能培养出形态均一的人大网膜ADSCs,经诱导后可向成熟脂肪细胞分化．     

脂肪源性干细胞在骨组织工程中的研究进展

脂肪源性干细胞由于其多系分化能力,在多项研究中发挥作用。其在组织工程中具有广阔的应用前景尤其是作为骨组织工程的新型种子细胞,可诱导成骨,应用于临床骨缺损修复。本文就脂肪源性干细胞在骨组织工程中的生物学特性及应用展开综述。     

Role of Stem Cells Transplantation in Tissue Regeneration After Acute or Chronic Acetaminophen Induced Liver Injury

Purpose: Acetaminophen-induced liver injury (APAP) is recognized as a frequent etiologic factor responsible for hepatic damage in the developed world. Management remains still elusive as treatment options are limited and their results are inconclusive. Consequently new strategies are explored at the experimental level. Mesenchymal stem cells (MSCs) present a promising modality as they can promote liver regeneration (LG) and compensate acute liver injury (ALI).Materials and methods: Our research was focused on articles related to drug-induced liver injury, mechanisms of liver regeneration (LG) after Acute Liver Injury (ALI) and recent experimental protocols of Mesenchymal Stem Cells (MSCs) transplantation after chemical insult. All these studies are cited on Pubmed and MedLine. Results: This review has three distinct sections. First recent developments in ALI pathogenesis are presented. The second section covers cellular pathways and histological findings relevant to liver regeneration. The final chapter analyzes MSCs transplantation protocols after ALI and interrelation between liver regeneration and hepatic differentiation of MSCs. Conclusion: Adipose tissue stem cells (ADSCs) and (MSCs) transplantation represents a promising modality in severe ALI management although many aspects remain to be clarified.     

脂肪源性干细胞修复周围神经损伤潜能的研究

目的:体外原代培养脂肪源性干细胞(adipose-derived stem cell,ADSC),并在细胞水平检测各种神经营养因子(neurotrophic factor,NF)的表达情况,探讨ADSC是否可以作为神经损伤修复的种子细胞。方法:体外原代培养和鉴定ADSC,应用RT-PCR方法检测ADSC细胞神经营养因子,包括神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、神经营养素-3(neurotrophin 3,NT-3);免疫组织化学法检测粘附因子(nerve cell adhesion molecule,NCAM)表达情况。结果:ADSC可以向脂肪和骨两方面分化;RT-PCR结果显示ADSC细胞NGF、BDNF、NT-3 mRNA水平明显升高;同时免疫荧光法检测到了高水平的NCAM。结论:ADSC具有干细胞分化潜能,并且能够表达NF和NCAM。     

绿色荧光蛋白标记的犬脂肪干细胞体外复合珊瑚材料的生长特性

目的研究犬脂肪干细胞(AdiposeDividedStromalCells,ADSCs)作为种子细复合珊瑚材料体外培养的生长特性。方法分离犬ADSCs,成骨诱导并转染绿色荧光蛋白(GFP)后,复合珊瑚材料培养。检测细胞总数,激光共聚焦观察细胞形态。结果ADSCs-珊瑚复合物体外增殖良好。激光共聚焦显示培养2周后细胞呈老化形态。结论GFP转染不影响细胞传代、增殖。细胞-材料复合物体外培养7天后回植较为适宜。