标准桃金娘油减轻PM2.5致大鼠气道黏液纤毛的损伤

目的观察大气细颗粒物(PM2.5)致大鼠气道黏液纤毛清除系统的损伤作用及标准桃金娘油干预的影响。方法将大鼠分成对照组(N组)、PM2.5暴露组(PM组)、标准桃金娘油干预组(PM+CS组),分别予以吸入净化空气、暴露石家庄室外空气(PM2.5浓度的范围29.90 mg/m^3~99.65 mg/m^3)、标准桃金娘油混悬液300 mg/(kg·d)灌胃。于3和6个月各处死大鼠8只,气管及肺组织行HE和AB-PAS染色;扫描电镜观察纤毛超微结构;计数BALF中细胞及分类;RT-qPCR测定黏蛋白(MUC)5AC mRNA和水通道蛋白(AQP)5 mRNA表达;免疫组化、Western blot测定MUC5AC蛋白表达。结果PM组肺及气管组织炎性损伤及黏液细胞化生明显;气管纤毛黏连、倒伏及缺失、排列稀疏、紊乱,BALF中白细胞总数、中性粒细胞计数和中性粒细胞百分比显著高于N组(P<0.05);MUC5AC mRNA相对表达量显著高于N组(P<0.05);AQP5 mRNA相对表达量显著低于N组(P<0.05);MUC5AC蛋白表达显著高于N组(P<0.05)。与PM组相比,标准桃金娘油可减轻上述损伤作用(P<0.05)。结论标准桃金娘油可通过减轻PM2.5致大鼠的纤毛结构损伤、黏液高分泌和炎性损伤作用,对黏液纤毛清除系统起保护作用。     

Numerical Modelling of the Dynamic Voltage in HTS Materials under the Action of DC Transport Currents and Different Oscillating Magnetic Fields

The dynamic voltage is a unique phenomenon of superconducting materials. It arises when the superconductor is carrying a DC transport current and spontaneously in subject to an AC magnetic field. This study excavates the aspects that previous studies have not comprehensively investigated: the dynamic voltage in a DC-carrying superconducting tape exposed to different oscillating AC magnetic fields. First, the fundamental physics of dynamic voltage/flux of superconductors is reviewed and further analysed in detail. We used the superconducting modelling method using the H-formulation merged into the finite-element method (FEM) software, to re-produce the typical dynamic voltage behaviour of a superconducting tape. The modelling was verified by both the analytical and experimental results, in order to precisely prove the reliability of the modelling. Afterwards, the modelling was performed for a DC-carrying superconducting tape under four different oscillating magnetic fields (sine, triangle, sawtooth and square), and their corresponding dynamic voltages and energy losses were analysed and compared. Results show the sinusoidal magnetic field can lead to the optimal combination of reasonable dynamic voltage but relatively lower loss, which is suitable for those superconducting applications requiring dynamic voltage as the energy source, e.g., flux pumps. This article presents novel investigation and analysis of the dynamic voltage in superconducting materials, and both the methodology and results can provide useful information for the future design/analysis of superconducting applications with DC transport currents and AC magnetic fields.     

CEREC AC全瓷修复系统的临床效果研究

目的 探讨椅旁CAD/CAM CEREC AC全瓷修复系统在牙体缺损修复中的应用和效果。方法 观察自2012年8月至2014年7月期间,采用CEREC AC全瓷修复系统所修复的70个病例,其中瓷嵌体10颗,全瓷冠60颗,由同一医生利用改良的美国公共健康协会修订标准（USPHS）对修复后6个月及12个月修复体的外形、边缘适合性、颜色匹配性、边缘染色、固位、修复后敏感性、牙龈健康及继发龋等方面进行评价。结果 70件修复体在观察期内保存完整,边缘密合,无明显松动、脱落、折断、继发龋、明显的牙齿敏感以及牙龈炎症等不良现象发生,修复后6个月及12个月的临床评价结果对比无统计学差异（P>0.05）。结论 CEREC AC全瓷修复系统可以高效地应用于牙体缺损的修复治疗,基于此方法制作出的修复体能达到令人满意的临床效果。     

siRNA沉默MUC5AC基因对胆管癌HCCC-9810细胞生长的影响

目的探讨特异性MUC5AC-siRNA沉默MUC5AC基因后对人肝内胆管癌细胞株HCCC-9810生长能力的影响。方法设计合成三对特异性siRNA,构建了三个稳定表达质粒,pRNAT-U6.1/Neo-MUC5AC-siRNA1/2/3,应用脂质体转染技术分别将三个稳定表达的质粒和对照质粒（空质粒对照）转染HCCC-9810,RT-PCR检测MUC5AC基因mRNA水平;SABC免疫组化染色技术检测MUC5AC粘蛋白的表达;MTT检测细胞生长增殖情况。结果基因测序表明成功构建质粒;转染后RT-PCR结果表明在mRNA水平,三个质粒都可抑制MUC5AC基因的表达,并可使MUC5AC的表达下降,MTT结果对细胞的生长有一定的抑制作用。结论构建pRNAT-U6.1/Neo-MUC5AC-siRNA1/2/3质粒明显抑制了MUC5AC基因在mRNA水平及蛋白的表达,并可抑制肿瘤细胞的生长增殖,为探讨MUC5AC在MUC5AC相关肿瘤的基因治疗方面奠定了一定的理论基础。     

Increased gene and protein expression of the novel eNOS regulatory protein NOSTRIN and a variant in alcoholic hepatitis

BACKGROUND & AIMS: Increased intrahepatic resistance in cirrhosis is associated with reduced endothelial NO synthase (eNOS) activity and exacerbated by superimposed inflammation. NOSTRIN induces intracellular translocation of eNOS and reduces NO generation. Our aims were to quantify and compare hepatic expression of eNOS, NOSTRIN, NOSIP, and caveolin-1 in alcoholic cirrhosis with or without superimposed alcoholic hepatitis and in normal livers. METHODS: Biopsy specimens from 20 decompensated alcoholic cirrhotic patients with portal hypertension (10 with alcoholic hepatitis) and 6 normal livers were analyzed: real-time polymerase chain reaction for quantification of messenger RNA; Western blotting; and enzyme assays of eNOS in normal and diseased liver were performed. Localization and interaction of eNOS and NOSTRIN in liver was assessed by immunohistochemistry and co-immunoprecipitation. RESULTS: eNOS mRNA was significantly increased and eNOS activity decreased in alcoholic hepatitis patients, despite no differences in eNOS protein expression among the patients. Patients with alcoholic hepatitis had significantly higher hepatic levels of NOSTRIN and caveolin-1 mRNA compared with cirrhosis alone or normal biopsy specimens. A NOSTRIN splice variant, not present in normal tissue, was detected on mRNA and protein levels in all alcoholic patients. Coimmunoprecipitation demonstrated association among NOSTRIN, eNOS, and caveolin-1. CONCLUSIONS: An increase in mRNA and protein of NOSTRIN and its shortened variant in alcoholic hepatitis may partly account for the paradox of increased mRNA levels and normal protein expression but decreased enzymatic activity of eNOS in diseased liver. Such intracellular regulators of NO production may be important in the development of increased intrahepatic resistance in alcoholic hepatitis patients.     

Hepatoprotective effects of Artemisiae Capillaris Herba and Picrorrhiza Rhizoma combinations on carbon tetrachloride–induced subacute liver damage in rats

The hepatoprotective properties of Artemisiae Capillaris Herba (AC) and Picrorrhiza Rhizoma (PR) are well known. The aim of this study was to determine the optimal composition of AC and PR mixtures for better complimentary or alternative regimens in reducing the level of hepatic fibrosis. Ten weeks of carbon tetrachloride injections caused subacute hepatic damage, manifested as significantly less body weight gain and hepatic protein content, and a higher liver weight, serum aspartate aminotransferase and alanine aminotransferase levels, hepatic malondialdehyde (an index for lipid peroxidation), and hydroxyproline (an index for collagen synthesis) concentrations. The carbon tetrachloride–induced toxic effects were inhibited by 11 different AC/PR mixtures as well as the single AC or PR treatment. More favorable effects were detected in all mixed-formulation groups compared with the AC or PR single formulations. Moreover, the AC/PR 2:1 formulation showed the most favorable hepatoprotective activity. The AC and PR mixtures showed good synergic hepatoprotective activity that was attributed to increasing free-radical scavenging ability. Among the 11 types of mixed formula tested in this study, the AC/PR 2:1 formulation had the most impressive synergic effects on inhibiting the subacute hepatic damage induced by carbon tetrachloride in rats and showed more favorable effects than with an equal dose of silymarin.     

近视眼LASIK术后AC/A比率的变化

目的研究近视眼患者准分子激光原位角膜磨镶（laser in sfiu keratomileusis，LASIK）后调节性集合（accommodative convergence,AC）与调节（accommodation,A）比率的变化。方法将230眼按近视屈光度等效球镜值-1．50～-3．00D、-3．25～-6．00D和-6．25～-11．00D分为A、B、C3个组行LASIK手术。采用同视机测定患者术前戴镜、术后1个月、3个月裸眼Ac／A值，并进行统计学分析。结果A、B、C3组术前戴镜AC／A值分别为3．02±0．79、3．03±0．69和2．71±0．47；术后1个月裸眼AC／A值分别为2．77±0．33、2．40±0．33和2．49±0．40；术后3个月裸眼AC／A值分别为3．04±0．74、2．79±0．58和2．74±0．60。术后1个月裸眼AC／A值明显低于术前戴镜和术后3个月，有显著性差异（P〈0．01）；术后3个月裸眼Ac／A值与术前戴镜Ac／A值比较无显著性差异（P〉0．05）；不同近视程度间各组AC／A值比较术前戴镜、术后1个月和术后3个月均无统计学差异（P〉0．05）。结论对于长期戴镜患者。近视程度对术前戴镜、术后裸眼Ac／A值均无影响；不同近视程度患者LASIK术后裸眼AC／A值的变化趋势相同，即均表现为先下降。后上升，至术后3个月时基本恢复到术前戴镜水平。[眼科新进展21107；27（2）：120-122]