警惕儿童心理病“从口而入”

儿童孤独症患呈增多的趋势，已引起很多学的关注。研究人员发现，儿童孤独症的发生、发展与过量食用“酸性食物”密切相关。高脂肪、高糖和动物性食物中的磷、硫、氯等在人体内易于形成酸性物质，故有人把富含磷、硫、氯等成分的食物统称为“酸性食物”。若过量食用肉类、糖类等酸性食品，则易使血液等体液酸性化，呈现“酸性体质”。     

幽门螺杆菌CagA及其致病作用的研究进展

CagA是幽门螺杆菌最重要的毒力因子之一，CagA阳性的Hp菌株同胃炎、萎缩性胃炎、十二指肠疾病和胃癌等消化道疾病相关。本文综述了Hp的毒力因子CagA的基因组、CagA定植于胃上皮细胞、CagA酪氨酸磷酸化位点、CagA入胞的靶向以及CagA多态性、毒性与胃癌的关系的近年研究状况。     

Effect of curcumin on nitric oxide synthase expression in Iipopolysaccharide-activated microglia cells and the anti-oxidative effect of curcumin

BACKGROUND: It has been demonstrated that curcumin can increase the activities of various anti-oxidase in blood and tissue, effectively eliminate various free radicals, reduce the production of peroxisome, and alleviate oxidative stress reaction. Whether it has the same effect on microglia? OBJECTIVE: To observe the effects of curcumin on the expressions of inducible nitric oxide synthase (iNOS), nuclear factor-κB (NF-κB), and superoxide dismutase (SOD) in microglial cell line BV stimulated by lipopolysaccharide (LPS). DESIGN: An observational comparative study. SETTING: Research Room of Biochemistry, Medical College of Nantong University. MATERIALS: Mice microglia cell line BV, iNOS and NF-κB reporter gene plasmids were presented by Dr. Bhat.NR. from the Medical University of South Carolina (USA). Curcumin was produced by the Xi'an Branch of China Chengdu Scholar Bio-Tech. Co.,Ltd.; LPS (E.Coli O26:B6), anti-mice iNOS monoclonal antibody, horseradish peroxidase labeled goat-anti-mice IgG were the products of Sigma Company (USA). METHODS: The experiments were carried out in the Research Room of Biochemistry, Medical College of Nantong University from May 2006 to April 2007. ① Detection of iNOS: The cells were seeded onto 24-well plate at the density of 1×105, After the cells had adhered to the cover glasses, the cells were grouped as negative control group (the primary antibody was replaced by phosphate buffered solution PBS); normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours). The expressions of iNOS protein were detected with immunocytochemical staining. ② Determination of iNOS and NF-κB gene activities: According to the introduction of the kit for transfection, iNOS or NF-κB report gene plasmids were transiently transfected with LipofectamineTM2000 liposomes into the cells in the 24-well plate for 24 hours. The cells were divided into normal control group (the cells were normally cultured after transfected with report gene plasmids); blank plasmid group (the cells were normally cultured after transfected with blank plasmids); LPS-treated group (the cells were treated with LPS for 4 hours after transfected with report gene plasmids); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours after transfected with report gene plasmids). The content of luciferase in the cell lysis buffer was determined after cell lysis. ③ Determination of SOD activity: The cells were seeded into culture bottle at the density of 1×106, and the divided into four groups, including normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours); vitamin C+LPS group (the cells were treated with vitamin C for 1 hour and LPS for 24 hours). The SOD activity was determined with xanthine oxidase and quantitative colorimetric assay. MAIN OUTCOME MEASURES: The expressions of iNOS protein, iNOS and NF-κB, and the activity of SOD were observed. RESULTS: ① Expression of iNOS protein in microglia: The expression of iNOS protein in the LPS-treated group was obviously higher than that in the negative control group (P < 0.01); Those in the curcumin+LPS group were significantly decreased as compared with that in the LPS-treated group (P < 0.01). ② Expressions of iNOS and NF-κB genes: The expressions of iNOS and NF-κB genes in the LPS-treated group were significantly higher than those in the normal control group (P < 0.01); Those in the curcumin+LPS group were significantly lower than those in the LPS-treated group (P < 0.01). ③ SOD activity: The activity of SOD in the LPS-treated group was significantly lower than those in the normal control group (P < 0.01). It in the curcumin+LPS group and vitamin C +LPS group was significantly higher than that in the LPS-treated group (P < 0.01). CONCLUSION: Curcumin could inhibit the expression of iNOS in the activated microglia, and it also has the abilities in eliminating free radicals and antagonizing lipid peroxidation.     

不典型伤寒33例临床分析

现将我们于 1996年 3月至 2 0 0 0年 2月收治的不典型伤寒 33例 ,分析报道如下。1　临床资料1.1　一般资料 :男 2 0例 ,女 13例 ,年龄 3～ 6 0岁 ,2 4例发病于 6～ 9月份。成人多不伴发热 ,而多以头痛、头闷、纳差、胃部不适、反复感冒为主要症状就诊 ,儿童多为不规则发热 ,均有按上呼吸道感染、胃病等一般治疗无效的病史。1.2　实验室检查 :白细胞总数 2 8例 <1× 10 9/L,约占 80 % ,其中 11例低于正常 ,嗜酸性粒细胞计数 <5 0× 10 6 /L ,2 7例 ,约占 80 % ,肥达反应 H凝集价、O凝集价均 >1∶ 16 0 ,9例PB、PA >1∶ 16 0 ,3例血沉增快…     

血小板活化因子拮抗剂对抗原引起气道嗜酸性粒细胞…

为探讨血小板活化因子（ＰＡＦ）拮抗剂消除哮喘气道炎症的价值，应用鸡卵清蛋白致敏和刺激小鼠复制过敏性气道炎症模型，研究ＰＡＦ拮抗剂对于抗原引起气道哮酸性粒细胞（ＥＯＳ）浸润的影响。体内实验结果表明，正常小鼠支气管肺泡灌洗液（ＢＡＬＦ）中未见到ＥＯＳ；致敏小鼠给予抗原多次反复吸入刺激后，ＢＡＬＦ中ＥＯＳ急剧增多。在ＰＡＦ一种选择性拮抗剂ＹＭ－２６４治疗各组中，ＹＭ－２６４的不同剂量０．１ｍｇ／ｋｇ、１     

长沙市开福区血吸虫病疫情调查分析报告

血吸虫病是血吸虫寄生在门静脉系统所引起的疾病。人主要是通过皮肤接触含尾蚴的疫水而感染,主要病变是由腹泻或排脓便血,血中嗜酸性粒细胞显著增多,慢性期以肝脾大为主,晚期以肝门静脉周围纤维化为主,可发展为门静脉高血压,巨脾与腹水。2005年湖南省血吸虫病形势依然不容乐观,至2004年底,全省尚有10多万血吸虫患者,钉螺面积2006万m2。所以各地有明确责任,力争在2007年达到全省控制血吸虫病的目标。调查钉螺不仅是制定灭螺规划和消灭钉螺的依据而且是考核灭螺效果、研究血吸虫病流行病学必须的工作。调查钉螺一般在上半年3、4、5月和下半年9…     

毛母质瘤1例

患者男 ,12岁 ,因“发现右手腕上部无痛性包块 3年”于2 0 0 4年 7月 16日入院。入院查体 :右手腕背侧触及约 3.0cm× 3.0cm× 2 .0cm大小包块 ,质硬 ,边界清楚 ,稍隆起 ,可活动 ,呈分叶状 ,无压痛。经局麻完整切除包块后切开见其内有一囊腔 ,内有灰黄色结节。术后患者恢复好 ,     

健康之路自己走(五)适量运动健康长寿 (下)

选择好运动时间冬季9点以后,夏季7点以后,阳光充足,气温合适,身体适应能力最佳;15-17点则为最宜时间.多数中老年人喜欢晨练,最好在起床1小时以后,由于睡眠时心率慢,血黏度高,骨关节、肌肉不够灵活,神经反应较慢,易出现外伤和心脑血管意外.清晨空气质量差,尤其阴天和大雾时,空气中含大量的有害物质,如细菌、病毒、酸性物质、氨、苯等,刺激呼吸道,易引发上呼吸道感染.