阴道粘膜上皮细胞的分离培养和鉴定

目的 初步建立阴道粘膜上皮细胞体外培养方法,为阴道粘膜上皮研究提供实验模型.方法 取雌性新西兰大白兔阴道粘膜组织小块,胶原酶Ⅳ和胰蛋白酶联合消化分离法收集上皮细胞,接种于角朊细胞无血清培养液中静置培养、传代.动态观察细胞生长增殖情况,扫描和透射电镜观察超微结构,流式细胞仪测定细胞增殖周期,并进行免疫组织化学鉴定.结果 体外培养的阴道粘膜上皮细胞为二倍体细胞,增殖状态良好,细胞间可见桥粒连接,免疫组化角蛋白染色阳性.细胞的超微结构和免疫组化染色均具有上皮细胞特征.结论 在本实验条件下,体外培养的阴道粘膜上皮细胞具有较好的增殖能力,可作为阴道粘膜上皮研究的理想实验模型.     

厨房卫生误区知多少

1.自来水隔夜用易得“怪病”与腹痛:生活中,不少人都习惯清晨一打开水龙头就用水刷牙、洗脸、做饭,更有甚者,直接饮用自来水。最新的研究却发现,隔夜水龙头往往窝藏有一种名为嗜肺军团杆菌的细菌,人若感染了这种细菌,就会得一种症     

泻血疗法加高频电针点剌引流治疗痤疮135例

目的：观察泻血疗法加高频电针点刺引流治疗痤疮患者的疗效。方法：治疗组135例采用泻血疗法加高频电针点刺引流综合治疗，取穴大椎，肺俞（双），膈俞（双）；对照组150例采用单纯点刺泻血疗法。结果：治疗组治愈率93．3％，对照组治愈率76．0％（P〈0．01）。提示：泻血疗法加高频电针治疗痤疮患者，使用高频电针点刺局部，早期清除瘀滞于痤疮内的分泌物及脓血，可防止炎症侵犯真皮遗留斑痕，同时由于瞬间高温可杀茵消炎而治标。     

多层螺旋CT在鉴别孤立性肺结节中恶性肿瘤的评价

严格意义上讲,孤立性肺结节（solitary pulmo-nary nodule,SPN）是指全肺内唯一的2～30 mm边界清楚的类圆形病灶.但在实际工作和本文叙述中,将呈毛刺状轮廓的孤立病灶也包括在内.在所有SPN中,恶性肿瘤约占40%,良性病变约占60%.但这种比例会受普查人群中肉芽肿性疾病的地方特性和受检人群以及检查方法的影响.就年龄而言,＜35岁患者SPN的恶性肿瘤可能性只占1%～3%,40岁患者良、恶性的概率几乎各占50%.     

PET/CT在评价及观察大鼠肺癌放疗效果中的应用

Objective The aim of this study was to understand the role of PET/CT in monitoring the therapeutic effect of radiotherapy (RT) on lung cancer with Wistar rats. Methods Thirty Wistar in-bred strain rats (6-8 weeks, weighed 180-280 g, female, ordinary) were made into Lewis pulmonary tumor model rats. 18F-fluorodeoxyglucose (FDG) PET/CT was performed when tumor reached 1.5-2.0 cm in greatest diameter (4-6 weeks) as a baseline. In order to get the optimal time point of PET/CT for moni-toring RT effect in rat cancer model, PET/CT was performed at the 3rd day, 1st, 2nd, 3rd, and 4th week after giving single dose of 5 Gy to each rat. Standardized uptake values (SUV) from FDG PET were measured and rats were sacrificed at different time point for validation. Besides, the expressions of glucose transport1 (Glut1) in tumor tissue were studied using immunohistochemistry. The level of tumor cell apoptosis, degra-dation, and necrosis were observed. SPSS 11.0 software was used for data analyses. Results A negative correlation of SUV uptake and time after RT and negative correlation of Glut1 expression and time after RT were observed in rat tumors, respectively. Positive correlation of SUV uptake and Glut1 expression in rat tumors was observed (Spearman rank correlation test, rs = 0.97, P < 0.01). Before RT, the SUV in rat tumor was 1.28 ± 0.31 and was decrease to 1. 00 ± 0.23 at the 3rd day and 0.18 ± 0. 10 at the 4th week after RT (F=15. 126, P<0.05). Before RT, the Glut1 in rat tumor was 0.2558 ±0.03 and was decrease to 0. 2320 ± 0. 01 at the 3rd day and 0. 1320±0.04 at the 4th week after RT. The amounts of tumor cell apopto-sis, degradation, and necrosis increased with time after RT. Conclusion Though FDG PET could monitor the therapeutic effect at the 3rd day after giving single dose of RT to rat lung tumor model, the optimal time was the the 4th week after treatment.     

小儿肺炎支原体脑炎15例临床分析

肺炎支原体(MP)是小儿呼吸道感染的重要病原之一,有多种并发症,其中中枢神经系统损害是MP所致肺外疾患中最常见类型,于2003年1月2004年12月收治住院肺炎支原体感染患儿182例,其中并发脑炎者15例,占8.2%,现报道如下:     

肺间变性大细胞淋巴瘤1例

病人,男,21岁.咳嗽、咳痰、痰中带血2月余;抗感染治疗无效.查体:左、右锁骨上分别可触及约1.5 cm×1 cm肿大的淋巴结,无压痛,质硬,活动度可,表面光滑.右侧胸廓触觉语颤减弱,右肺呼吸音较低.胸部X线正侧位片:右肺上野透过度降低,内中带可见均匀致密阴影,边缘不规则.     

血液紫外线照射充氧治疗家兔梭曼急性中毒

目的 探讨紫外线照射充氧(UBIO)对梭曼急性中毒家兔全血乙酰胆酯酶(AchE)活性的影响及对梭曼中毒后肺损伤的治疗作用.方法 家兔40只,随机分为正常对照组、中毒组、常规治疗组及UBIO治疗组各10只,于治疗后2h检测全血AchE 活性及进行动脉血气分析.收集支气管肺泡灌洗液(BALF),分析其中碱性磷酸酶(AKP)、酸性磷酸酶(ACP)活性.结果 中毒组AchE活性显著低于对照组(P<0.01),BALF中AKP及ACP活性高于对照组(P<0.01).经UBIO治疗后AchE活性升高,且明显高于中毒组及常规治疗组.与中毒组比较,UBIO治疗组中AKP及ACP活性显著降低(P<0.05),而动脉血pH值、PaO2及SaO2明显升高(P<0.05),PaCO2明显降低(P<0.05).结论 UBIO能提高全血AchE活性及减轻梭曼中毒后的肺损伤,因而可能是一新的治疗梭曼急性中毒的途径.