刺玫果对老龄小鼠衰老指标SOD、MDA的影响作用

目的 :研究刺玫果提取物 (喷雾干燥粉 )对衰老小鼠脑、肝组织中超氧化物歧化酶 (SOD)和丙二醛(MDA)含量的影响。方法 :SOD测定为黄嘌呤氧化酶法 ,MDA测定为硫代巴比妥酸法。结果 :刺玫果提取物可显著增强老龄小鼠脑和肝SOD活性 ,降低MDA含量。结论 :刺玫果提取物具有一定的抗衰老作用     

免疫学

反复生化妊娠流产的淋巴细胞免疫治疗38例分析；个体化免疫抑制-抗凝疗法在抗心磷脂抗体阳性复发性流产患者治疗中的应用；多重免疫缺陷NOD／SCID小鼠CD3^＋DX5^＋细胞亚群与妊娠结局的关系；抑制Her-2基因表达对NK-92细胞杀伤SKOV3细胞活性影响的研究；hCGβ-C3d3融合蛋白在乳酸杆菌的表达及鉴定。     

脑缺血预处理对脑缺血再灌注损伤小鼠纹状体caspase-3活性的影响

短暂的缺血（预处理）可减轻随后严重、长时间缺血导致的损伤，这一现象称为缺血耐受。缺血耐受最初发现于心脏，随后在脑和肝脏等器官也相继发现。大鼠短暂前脑缺血导致再灌注24 h时海马锥体细胞csspase-3 mRNA水平明显升高，至再灌注72 h仍维持较高水平，但至再灌注96 h时caspase-3 mRNA水平明显下降，有可能发生变性。     

Effect of Some Thiol Chelators on Enzymatic Activities in Blood,Liver and Kidneys of Acute Arsenic（Ⅲ）Exposed Mice

The effects of meso 2,3-dimercaptosuccinic acid(DMSA),sodium 2,3-dimercaptopropane 1-sulfonate(DMPS)and S-adenosyl L-methionine(SAM)on the enzymatic acivities of mice were studied.The mice were given intraperitoneal(i.p.)injections of these chelating agents(1 mmol/kg) and 3 h later the activity of δ-aminolevulinic acid dehydratase(ALAD) in the blld,and aspartate aminotransferase(AST),alanine aminotransferase(ALT),γ-glutamyltranspeptidase(γ-GT）,alkaline phosphatase(ALP)in the liver and kidney were determined.The activity of blood ALAD was significantly increased by the administration of DMSA and SAM while DMPS had only a moderate effect.The activities of other hepatic enzymes changed little the mice were treated with these chelating agents,except for a significant reduction in hepatic ALP activity following DMPS administration.Arsenic(Ⅲ） administration markedly increased the activities of ALT and ALP in the liver and kidneys.The changes in the enzymatic activities by treatment with arsenic were prevented by injection of DMSA,DMPS and SAM,DMSA being the most effective.These results indicate that DMSA,DMPS and SAM wer not toxic to the liver or kidnys of mice and that treatment with DMSA is more effective than DMPS or SAM in protecting mice from acute hepatic or renal toxicity caused by arsenic.     

三种不同品系小鼠在肺肿瘤短期诱发试验中的敏感性比较

用不同剂量尿烷一次腹腔注射，对ＫＭ、ＢＡＬＢ／ｃ和Ａ／Ｊ系小鼠进行肺肿瘤短期诱导试验，以比较其敏感性，为环境化学物致癌性鉴定的实验条件提供依据。结果显示：以肺肿瘤发生率和平均发瘤数两项指数来衡量发瘤危险，Ａ／Ｊ系均在最低染毒剂量（２５ｍｇ／ｋｇ）即出现发瘤危险明显增高；ＫＭ系以发生率衡量时，１００ｍｇ／ｋｇ为发瘤危险增高的最低剂量，而以平均发瘤数衡量时，最低剂量为２００ｍｇ／ｋｇ；ＢＡＬＢ／ｃ系前     

两种肿瘤细胞株对小鼠腹腔巨噬细胞产生一氧化氮的影响

目的：研究体外两种肿瘤细胞株对巨噬细胞产生一氧化氮的影响。     

SarCNU治疗荷人脑胶质瘤裸小鼠模型NHG—1的疗效观察

为探讨新型抗癌药SarCNU治疗人脑胶质瘤的疗效，采用腹腔给药，观察荷皮下和脑内胶质瘤裸小鼠模型体内肿瘤生长情况。结果：SarCNU能显著抑制肿瘤生长，延长荷瘤动物生存期。SarCNU与BCNU和VM26相比，具有更强的抑制肿瘤生长作用。     

益气活血解毒方诱导Lewis肺癌小鼠细胞凋亡的研究

目的：观察益气活血解毒方对Lewis肺癌小鼠肿瘤细胞凋亡的影响。方法：选用Lewis肺癌小鼠肿瘤细胞株，制作肿瘤动物模型后，分为盐水对照组、益气活血解毒方组，进行光、电镜形态学观察。     

哮喘小鼠中核因子-κB对Th2型细胞因子分泌的影响

支气管哮喘表现为Th/Th2型细胞免疫应答的平衡失调、Th1型细胞的免疫应答下调、Th2型细胞因子(如IL-4、IL5等)过度表达.