寡聚蛋白Trim34α对NF-κB报告基因的负向调节作用

目的 测Trim34α对TAB2诱导的NF-κB报告基因活化的影响.结果 经鉴定成功构建Trim34α真核表达载体,该载体表达的Trim34α蛋白能相互聚集形成寡聚体(Trim小体);Trim34α可显著抑制TAB2诱导的NF-κB荧光素酶报告基因活化.结论 Trim34α可以在胞内形成寡聚体,Trim34α能显著抑制TAB2诱导的NF-κB报告基因活化.

Abstract：

Objective To investigate the effects of Trim34α on the activation of luciferase reporter gene containing NF-κB promoter induced by adaptor proteins TAB2. Methods The total RNA was isolated from HeLa cells. After amplification with RT-PCR, the target sequences were cloned into 5'-Flag-pcDNA3.1 (+) vector. The recombinant vector was confirmed by restriction enzyme digestion, colony PCR and sequencing. It was transfected into HEK293T cells to detected Trim34α expression by Western blot. Simultaneously, the effects of Trim34α on the NF-κB activation induced by TAB2 were determined by dual-luciferase reporter assay. Results Restriction enzyme digestion, colony PCR and sequencing confirmed the vector was constructed successfully, furthermore it expressed Trim34α protein in HEK293T cells. Moreover, trim34α could form high-molecular-weight oligomeric protein, and here we called it trimsome. Interestingly, dual-luciferase assay showed that Trim34α could effectively block TAB2-induced NF-κB activation. Conclusion Trim34α was involved in negative regulation of TAB2-induced NF-κB activation and could form high-molecular-weight oligomer.     

αβ复合型地中海贫血的分子检测及血液学分析

目的通过对αβ复合型地中海贫血的分子基因型的检测及血液学的表型分析,了解其基因分布状况及发生率。方法采用单管多重缺口-PCR法和反向膜杂交法,对396例地中海贫血筛查阳性的样本进行α地中海贫血和β地中海贫血的基因诊断。结果在396例样本中,检出单纯性α地中海贫血109例(27.5%),单纯性β地中海贫血115例(29.0%),αβ复合型地中海贫血26例(6.57%)。αβ复合型地中海贫血占β地中海贫血的18.44%。αβ复合型地中海贫血共有7种基因型,涉及5种β地中海贫血突变类型(CD41-42、IVS-2-654、TA;TA;box-28、CD71-72、43)和2种α地中海贫血缺失类型(α地中海贫血-1基因和α地中海贫血-2基因)。红细胞参数(MCV、MCH及Hb)在αβ复合型地中海贫血与单纯性β-地中海贫血之间的差异不具有统计学意义(P>0.05)。结论αβ复合型地中海贫血双重杂合子的发生率较高,且缺乏特异性的血液学指标。对β地中海贫血筛查阳性的病人应同时进行α和β地中海贫血基因诊断,以减少αβ复合型地中海贫血漏检的可能,以便下一步正确地指导遗传咨询和产前诊断。     

瘤内注射MIP-3α对小鼠肝癌生长抑制作用的研究

目的:探讨瘤内注射巨噬细胞炎症蛋白-3α(macrophage inflammatory protein-3α,MIP-3α)能否趋化外周树突状细胞(Dendritic cells,DCs)至肿瘤组织内,诱导特异性免疫应答。方法:成功建立小鼠皮下肝癌模型后随机分为3组,第10天起分别向MIP-3α治疗组、PBS对照组的小鼠皮下肿瘤内注射MIP-3α溶液及PBS,空白对照组小鼠不予任何处理。20天后取肿瘤组织,免疫组化法检测肿瘤内DCs、CD4+、CD8+细胞浸润情况,流式细胞术检测肿瘤内DCs浸润数量及其表型。另外,每组各10只小鼠持续观察,用于绘制肿瘤生长曲线并观察生存时间。结果:①MIP-3α治疗组的小鼠肿瘤内浸润的CD4+、CD8+细胞及DCs数量均显著高于其他两组。②MIP-3α治疗组小鼠肿瘤内浸润性DCs的CD80、CD86表达率显著高于其他两组(P<0.05)。③MIP-3α治疗组小鼠肿瘤生长速率显著低于对照组(P<0.001),生存时间较对照组明显延长(P<0.05)。结论:①瘤内注射MIP-3α可在小鼠肝癌病灶内趋化、募集外周的树突状细胞,使其摄取并提呈肿瘤抗原,有效诱导针对肝癌细胞的特异性免疫应答。②MIP-3α在小鼠皮下肝癌模型的局部微环境下可能具有促进树突状细胞成熟的作用。     

低氧刺激鼻息肉黏膜上皮细胞炎性因子变化的初探

目的:研究低氧刺激慢性鼻窦炎伴鼻息肉（CRSwNP）与正常鼻黏膜上皮细胞炎性因子的变化与异同,探讨其在CRSwNP发病机制中的作用。方法:选择2015年6月至2018年1月在吉林大学中日联谊医院就诊的68例CRSwNP患者,其中男性36例,女性32例,年龄（45.2±12.5）岁（ xˉ±s,下同）,患者的...     

α7神经型尼古丁受体激动剂PNU282987对APP/PS1小鼠海马组织中DYN-Ⅰ蛋白表达的影响

目的研究特异性激动APP/PS1转基因小鼠脑组织中α7神经型尼古丁受体(α7 nAChR)水平后对海马组织DYN-Ⅰ蛋白的影响;探讨α7 nAChR在阿尔茨海默病(Alzheimer disease,AD)发病机制中的神经保护作用机制。方法实验动物分为对照组(Control)、野生加PNU282987组(WP)、APP/PS1转基因组(APP/PS1)和APP/PS1加PNU282987组(AP)各8只,WP和AP组给予α7 nAChRs特异性激动剂PNU-282987,另两组给予生理盐水,给药方式为小鼠24 w龄时1 mg/kg腹腔注射PNU-282987,连续5 d。采用Real-time PCR法和蛋白免疫印迹(Western Blot)法分别测定小鼠海马组织中发动蛋白Ⅰ(DYN-Ⅰ)mRNA和蛋白表达水平的变化。结果与control组相比,APP/PS1组海马组织中发动蛋白Ⅰ(DYN-Ⅰ)mRNA和蛋白水平下降(P0.05,P0.01);而特异性激动α7 nAChR水平后,与对照组相比WP组中发动蛋白Ⅰ(DYN-Ⅰ)mRNA和蛋白水平升高(P0.05,P0.01);与APP/PS1组相比AP组小鼠大脑海马组织中发动蛋白Ⅰ(DYN-Ⅰ)mRNA和蛋白水平明显升高(P0.01,P0.01)。结论特异性激动APP/PS1转基因小鼠海马组织中α7 nAChR水平能够使网格蛋白内吞调节蛋白DYN-Ⅰ表达升高。这可能提示了α7 nAChR对突触有一定的保护作用,进一步说明α7 nAChR在阿尔茨海默病的发病中起着重要作用。     

Cytokines and psychopathology: lessons from interferon-alpha.

Interferon-alpha is a potent inducer of the cytokine network and is notorious for causing behavioral alterations. Studies on interferon-alpha-treated patients reveal at least two distinct syndromes: 1) a mood/cognitive syndrome that appears late during interferon-alpha therapy is responsive to antidepressants and is associated with activation of neuroendocrine pathways and altered serotonin metabolism; and 2) a neurovegetative syndrome characterized by psychomotor slowing, and fatigue that appears early during interferon-alpha treatment is antidepressant nonresponsive and may be mediated by alterations in basal ganglia dopamine metabolism. Findings from interferon-alpha may provide important clues regarding the pathophysiology and treatment of cytokine-induced behavioral changes in medically ill patients, while also potentially modeling the development of neuropsychiatric symptoms in patients without medical disorders.     

大黄联合肠内营养对胃癌围手术期外周血TNF-α水平的影响

目的:观察胃癌患者围手术期使用中药大黄和肠内营养对TNF-α水平的影响。方法:将56名胃癌围手术期患者随机分为对照组(A组)20例;肠内营养组(B组)21例;大黄加肠内营养组(C组)15例。A组术后常规补液的同时实施肠外营养支持;B组术后常规补液的同时术后42h始给予肠内营养能全力,一直持续到术后第8天;C组病人术前1天应用大黄10g,术后18、42h分2次各5g。B组和C组术后数日内每天的液体量、热量及氮量不足部分均由肠外途径予以补足。所有研究对象分别于手术前1天、手术后第1、3、7天检测外周血肿瘤坏死因子(Tumor Necrosis Factorα,TNF-α)浓度。结果:各组患者术后均有急性炎性反应的发生,但C组术后第3、7天的TNF-α水平较A有显著下降,术后排气、排便时间和肠鸣音的恢复显著提前。术后的营养指标B组、C组患者间无明显差异。结论:胃癌患者围手术期应用大黄可以有效降低外周血TNF-α水平,缓解手术创伤引起的急性炎性反应,促进术后胃肠道功能恢复,有利于实施术后肠内营养支持。     

连翘化学成分研究

目的 研究连翘Forsythia suspense果实的化学成分.方法 采用硅胶色谱技术及重结晶等方法进行分离纯化,通过现代波谱技术鉴定化合物的结构.结果 从连翘80％乙醇提取后水沉部位分离得到1 1个化合物,分别鉴定为乙酰齐墩果酸(1)、异降香萜烯醇乙酸酯(2)、β-香树脂醇乙酸酯(3)、长管大青素A(4)、甲基-β-D-吡喃葡萄糖(5)、商陆种酸(6)、积雪草酸(7)、连翘苷(8)、齐墩果酸(9)、β-谷甾醇(10)、β-胡萝卜苷(11).结论 化合物1、4～7均是首次从该属植物中分离得到.     

Astrocytes in the damaged brain: Molecular and cellular insights into their reactive response and healing potential

Long considered merely a trophic and mechanical support to neurons, astrocytes have progressively taken the center stage as their ability to react to acute and chronic neurodegenerative situations became increasingly clear. Reactive astrogliosis starts when trigger molecules produced at the injury site drive astrocytes to leave their quiescent state and become activated. Distinctive morphological and biochemical features characterize this process (cell hypertrophy, upregulation of intermediate filaments, and increased cell proliferation). Moreover, reactive astrocytes migrate towards the injured area to constitute the glial scar, and release factors mediating the tissue inflammatory response and remodeling after lesion. A novel view of astrogliosis derives from the finding that subsets of reactive astrocytes can recapitulate stem cell/progenitor features after damage, fostering the concept of astroglia as a promising target for reparative therapies. But which biochemical/signaling pathways modulate astrogliosis with respect to both the time after injury and the type of damage? Are reactive astrocytes overall beneficial or detrimental for neuroprotection and tissue regeneration? This debate has been animating this research field for several years now, and an integrated view on the results obtained and the possible future perspectives is needed. With this Commentary article we have attempted to answer the above-mentioned questions by reviewing the current knowledge on the molecular mechanisms controlling and sustaining the reaction of astroglia to injury and its stem cell-like properties. Moreover, the cellular/molecular mechanisms supporting the detrimental or beneficial features of astrogliosis have been scrutinized to gain insights on possible pharmacological approaches to enhance astrocyte neuroprotective activities.     

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??Abstracts?? Objective To study the T2* magnetic resonance imaging for evaluation of iron deposition of liver?? pancreas and heart in β- thalassemia major ??β-TM?? patients and the relationship of the serum ferritin with iron overload. Methods Measurement of hepatic?? pancreatic and cardiac MRI T2* was performed in 153 patients with β-TM from May 1?? 2010 to December 31?? 2010??The demographics and serum ferritin ??SF?? were collected. The rate of the iron overload in liver?? pancreas and heart was analyzed. The rates of combined pancreatic and cardiac iron overload in different hepatic iron overload groups were compared. Results According to MRI T2*?? there was serious hepatic iron overload in 85 cases?? moderate in 39?? mild in 21?? no iron overload in 8. Similarly?? there was pancreatic iron overload in 134?? no iron overload in 19. In terms of cardiac iron overload?? there was serious cardiac iron overload in 37 cases?? mild in 21?? no iron overload in 95. No correlation was found between hepatic?? pancreatic and cardiac MRI T2* and age or SF?? but hepatic MRI T2* correlated with pancreatic and cardiac MRI T2*??P = 0.000?? r = 0.529?? r = 0.369???? and pancreatic MRI T2* correlated with cardiac MRI T2* well ??P = 0.000?? r = 0.715??. As grade of hepatic iron overload increases?? the patients demonstrated higher rate of combination of pancreatic and cardiac iron overload simultaneously ??χ2 = 20.78??P = 0.000??.