Autologous Dentin Graft after Impacted Mandibular Third Molar Extraction to Prevent Periodontal Pocket Formation—A Split-Mouth Pilot Study

This preliminary study aims to evaluate the efficacy of an autologous dentin graft in preventing periodontal defects after impacted or semi-impacted lower third molars’ (M3) surgical extraction. For this purpose, radiographic and periodontal evaluation of post-extractive sockets were performed. Ten patients were enrolled in the study: twenty M3 extraction sockets were treated with a split-mouth modality. After tooth extraction, the experimental sites were filled with autologous dentin graft obtained by the extracted M3, while the control sites were filled with blood clot alone. Flaps were closed by first intention to ensure the stability of the wounds. Post-extractive sites were monitored at days 15, 90 and 180. The healing was not affected by any complications associated with the use of the autologous dentine graft in all cases. The measurements recorded at six months showed a reduction of the probing pocket depth distal to the second lower molar (M2) at both surgical sites, with a greater reduction observed at the experimental sites. Radiographic evaluation also showed a greater amount of bone gain at the grafted sites compared to the control sites. The results of this preliminary study suggest that autologous dentin grafts can be useful in preventing the formation of periodontal defects distal to M2 after M3 surgical extraction.     

会阴Ⅲ～Ⅳ度裂伤及特殊裂伤的预防和处理

会阴Ⅲ～Ⅳ度裂伤是阴道分娩的严重并发症,由于裂伤累及肛门括约肌复合体和（或）肛门黏膜,又称为产科相关肛门括约肌损伤（OASIS）,是导致产后肛门失禁最常见的原因,严重影响生活质量。阴道分娩后准确评估会阴裂伤程度并及时修复解剖结构对恢复肛门括约肌功能、改善预后至关重要;但临床实践中隐匿性OASIS有时被漏诊,不能及时识别、修复或愈合不良等,可导致会阴-直肠瘘或直肠-阴道瘘。需要加强预防和管理会阴Ⅲ～Ⅳ度裂伤,恰当运用质量管理工具,分析导致OASIS的风险因素和关键环节,从妊娠期和产程管理、助产能力提升、风险评估、预防和治疗等关键环节着手,减少或避免OASIS发生,提升识别和修复OASIS的能力。     

两种冲洗法获取早孕胎儿滋养细胞方法比较

目的：通过对两种冲洗法在子宫下段获取滋养细胞的比较研究．探索一种适合于孕早期产前诊断的、安全无损伤的获取胎儿细胞的取样方法。方法：①对拟行人工流产的46例早孕妇行循环子宫腔冲洗,与38例单腔冲洗管样本进行比较,研究获取胎儿滋养细胞的情况。②对获取的滋养细胞行免疫组化鉴定。③对拟行药物流产的早孕妇行两种子宫腔冲洗．探索取样操作对孕妇及胎儿的短期影响。结果：双腔循环宫腔冲洗法比单腔冲洗法获取滋养细胞的例数高（73．9％．44．8％,P〈0．05）;APAAP免疫组化试验表明．多核细胞能与抗滋养细胞多克隆抗体及单克隆抗体发生强阳性反应;冲洗取样操作短期（3d）内观察未发生流产、感染、腹痛等异常请况。结论：①孕早期在官腔内可以收集到胎儿滋养细胞。免疫组化研究进一步证实它的性质。②双腔循环官腔冲洗法与单腔宫腔冲洗法相比．取样效果更优越。③冲洗法简便易行．短期观察对母儿是安全的。     

Retrospective Detection and Complete Genomic Sequencing of Canine morbillivirus in Eurasian Otter (Lutra lutra) Using Nanopore Technology

The Eurasian otter (Lutra lutra) is a piscivorous apex predator in aquatic habitats, and a flagship species of conservation biology throughout Europe. Despite the wide distribution and ecological relevance of the species, there is a considerable lack of knowledge regarding its virological and veterinary health context, especially in Central Europe. Canine morbillivirus (Canine distemper virus (CDV)) is a highly contagious viral agent of the family Paramyxoviridae with high epizootic potential and veterinary health impact. CDV is present worldwide among a wide range of animals; wild carnivores are at particular risk. As part of a retrospective study, lung-tissue samples (n = 339) from Eurasian otters were collected between 2000 and 2021 throughout Hungary. The samples were screened for CDV using a real-time RT-PCR method. Two specimens proved positive for CDV RNA. In one sample, the complete viral genome was sequenced using a novel, pan-genotype CDV-specific amplicon-based sequencing method with Oxford Nanopore sequencing technology. Both viral sequences were grouped to a European lineage based on the hemagglutinin-gene phylogenetic classification. In this article, we present the feasibility of road-killed animal samples for understanding the long-term dynamics of CDV among wildlife and provide novel virological sequence data to better understand CDV circulation and evolution.     

柳州地区2206例妊娠中期孕妇的产前筛查结果分析

目的分析柳州地区进行母血清产前筛查（以下简称产前筛查）的孕产妇中,胎儿有缺陷的发生情况。方法取孕中期孕妇血清进行甲胎蛋白、游离绒毛膜促性腺激素β-亚基及游离雌三醇等3项指标检测,用专用分析软件计算其风险率,并与B超、羊水、新生儿检查等结果对照。结果高风险孕妇检出率为9.0%,其中21-三体综合征（DS）为7.4%,18-三体综合征为0.4%,神经管畸形（NTD）为0.9%。结论产前筛查和产前诊断是减少出生缺陷发生的重要措施,具有明显的经济效益和社会效益。     

Intrauterine hematomas in the second and third trimesters associated with adverse pregnancy outcomes: a retrospective study

Objective: To carry out a retrospective study of the clinical features of patients with intrauterine hematoma in the second and third trimesters, and discuss the risk factors for poor pregnancy outcomes.

Methods: A total of 398 patients who underwent routine examination in our hospital from January 2011 to December 2015 were classified into normal pregnancy (NP) group (N?=?265) and adverse pregnancy (AP) group (N?=?133), according to their pregnancy outcomes. Maternal clinical demographics, gestational age, location of hematoma, volume of hematoma, and accompanying contraction and vaginal bleeding were recorded.

Results: The average age of pregnant women in the NP and AP groups was 28.25?±?4.06 and 29.5?±?5.06 years, respectively (p?=?0.007). Gestational age at first detection of hematoma was 15.11?±?5.13 weeks in the NP group compared with 21.22?±?8.25 weeks in the AP group (p?p?Conclusion: Intrauterine hematoma in the second and third trimesters is a sign of pathological pregnancy, resulting in adverse outcomes. Maternal age, gestational age at first diagnosis, location of hematoma and accompanying contraction are risk factors for poor pregnancy outcomes.