神经肽P物质对肉芽组织成纤维细胞增殖及表皮生长因子基因表达的作用

目的：探讨感觉神经肽P物质（SP）对离体培养的肉芽组织成纤维细胞的促增殖作用及其对表皮生长因子（EGF）基因表达的调控作用。方法：采用MTT法测定SP对肉芽组织成纤维细胞的促增殖作用；采用逆转录－聚合酶链反应（RT－PCR）方法检测SP对成纤维细胞EGF基因表达的调控作用，观察时间及剂量－效应关系。结果：10^-9～10^-5mol/L的SP在体外对肉芽组织成纤维细胞均具有明显的促增殖作用（P＜0．01），且具有明显的剂量依赖性（γ=0.594,P＜0．01），EGF抗体只能部分抑制这一作用（与对照组比较，P＜0．01；与10^-7mol/L SP组比较，P＜0．05）。10^-7mol/L SP可诱导成纤维细胞EGF mRNA的表达，在作用后6h与对照组比较，差异有非常显著性意义（P＜0．01）；SP在10^-8～10^-6mol/L范围内可以显著促进成纤维细胞EGF mRNA表达，在10^-7mol/L达到峰值，当浓度＞10^-7mol/L时，其促EGF mRNA表达的效应强度随浓度升高而有所降低，至10^-5mol/L时与对照组比较，差异无显著性意义。结论：SP对肉芽组织成纤维细胞具有明显的促增殖作用，这种作用与其诱导成纤维细胞EGF表达有关。     

P38MAPK抑制剂对缺血/再灌注大鼠肾脏功能损伤的保护作用

目的：观察P38MAPK抑制剂对缺血／再灌注大鼠肾脏功能损伤的保护作用。方法：夹闭肾动脉制遣大鼠肾脏缺血／再灌注损伤动物模型，静脉注射P38MAPK抑制剂阻断P38MAPK信号转导通路，测量其对肾功能及细胞因子含量的影响。结果：肾脏和血浆中TNF—α和IL-β含量随缺血／再灌注时间的延长而升高，肾功能损伤也随缺血／再灌注时间的延长而加重。应用P38MAPK抑制剂可显著降低TNF-α和IL-β含量，对缺血／再灌注所致的肾功能损伤具有明显改善作用。结论：P38MAPK抑制剂可通过抑制致炎因子的产生而减轻缺血/再灌注所致的大鼠肾脏功能损伤。     

Effects of Several Denervation Procedures on Distribution of Calcitonin Gene-Related Peptide and Substance P Immunoreactive in Rat Stomach

The effect of chemical deafferentation, vagotomy(VGX), and gangliosympathectomy (GSX) on the density offibers containing calcitonin gene-related peptide (CGRP)and substance P (Sub.P) in the rat gastric wall was studied. Chemical deafferentation bycapsaicin abolished the density of CGRP-immunoreactive(IR) fibers, not Sub.P-IR fibers. Ten days after VGX,the density of CGRP-IR or Sub.P-IR fibers in the mucosa was largely reduced, while no reductionof CGRP-IR and Sub.P-IR fibers was seen in submucosaland muscular layers. GSX significantly reduced thedensity of CGRP-IR fibers in the mucosa and caused a moderate decrease in the fibers in submucosaland muscular layers. Pretreatment with6-hydroxydopamine, a neurotoxin for noradrenergicnerves, did not affect the density of CGRP-IR fibers inthe gastric wall. The density of Sub.P-IR fibers in thegastric wall was not affected by GSX. These studiesindicate that the CGRP-IR and Sub.P-IR fibers in themucosa are susceptible to extrinsic nerve denervation compared with those in the submucosa and musclelayers, that a major portion of the CGRP-IR fibers inthe mucosa is of both vagal and spinal origin, and thata major portion of the Sub.P-IR fibers in the mucosa is of vagal origin. Furthermore, thepresent results support that CGRP-IR fibers, notSub.P-IR fibers, in the rat stomach arecapsaicin-sensitive.     

海风藤及其代用品药理作用的比较研究

选择经典的实验指标，对海风藤及其３种代用品山Ｊｕ，石楠藤有毛Ｊｕ的药理作用进行了比较研究，结果表明海风藤，山Ｊｕ和毛Ｊｕ在抗炎，镇痛和抗血小板聚集方面均有效，其中海风藤作用最强，其次为山Ｊｕ，毛Ｊｕ石楠藤没有明显的抗炎作用，其抗血小板聚集及止痛作用稍强于毛Ｊｕ。     

The isolated spinal cord-skin preparation of the newborn rat and effects of some algogenic and analgesic substances

We have developed an isolated spinal cord-skin preparation of the newborn rat. The spinal cord together with a piece of skin connected to the cord by the saphenous nerve was isolated from 1- to 4-day-old rats and separately superfused with artificial cerebrospinal fluid in two neighbouring chambers. Potentials were recorded extracellularly from the third lumbar ventral root. Application of capsaicin (0.5-2 μM) or KCl (60–350 mM) with brief pressure pulses to the perfusion bath of the skin evoked a depolarizing response of 20- to 40-s duration in the ventral root. The response was depressed by [Met⁵]enkcphalin (0.03–3 μM). morphine (0.1–2 μM) and a tachykinin antagonist, [D-Arg¹,D-Trp^7,9,Leu¹¹] substance P (spantide), 1–10 μM), applied to the spinal cord by superfusion, whereas the response was augmented by centrally administered calcitonin gene-related peptide (0.1–0.2 μM) or bicuculline (0.5–1 μM).     

Integration of new regulatory strategies into the network of an endocrine control system: limitation of androgen secretion by rat testis is achieved by substrate-dependent modulation of P450XVII enzyme concentration and catalytic efficiency.

In addition to the well-known control circuits involved in the regulation and adaptation of testicular androgen biosynthesis, it is proposed that two new control strategies are involved in the maintenance of steady-state testosterone secretion rates by testicular Leydig cells. Cytochrome P450XVII (steroid-17 alpha-monooxygenase/steroid-17,20-lyase), one key enzyme in steroid hormone biosynthesis, responds to external human choriogonadotropin stimulation with an oxygen-dependent and substrate flux-dependent inactivation and decomposition, and increased substrate availability decreases the efficiency of androgen formation in favour of abortive intermediate leakage. These results are discussed as a paradigm of substrate-dependent modulation of cytochrome P450 activities.     

细胞色素P450调节剂对DNA加合物形成的影响

人羊膜上皮细胞ＦＬ系分别接触ａ－萘黄酮（０．６ｍｍｏｌ·Ｌ￣（－１））β－萘黄酮（２０ｐｍｏｌ·Ｌ￣（－１））２４ｈ后，再用苯并（ａ）芘［Ｂ（ａ）Ｐ，１０ｕｍｏｌ·Ｌ￣（－１）］处理２４ｈ，用３２Ｐ后标记技术测定以Ｂ（ａ）－ＤＮＡ加合物。结果发现，阳性对照组，ａ－萘黄酮预处理组及β－萘黄酮预处理组加合物的量分别为（加合物个数／１０’个核苷酸）：４．７±０．２（１００％），１．８±０．９（３８．３％），１６．０±２．２（３４０．１％）．该实验结果直接显示了纳胞色素Ｐ４５０调节剂对肿瘤发生影响的作用水平。亦为药物对致癌物代谢影响的研究提供了一种方法．     

Effects of systemic clonidine on auditory event-related potentials in squirrel monkeys

Event-related potential (ERP), electroencephalographic (BEG), and behavioral data were collected from squirrel monkeys (Saimiri sciureus) in a 90−10 auditory oddball paradigm. Background or target tones were presented once every 2 s, and responses to the targets were rewarded. ERPs were recorded from epidural electrodes following systemic administration of clonidine (0.1 mg/kg) or a saline placebo. EEG power spectra and behavioral performance were assessed simultaneously as indices of behavioral state. Clonidine significantly decreased the area and increased the latency of a P300-like potential. The amplitudes and areas of the earlier P1, N1, and P2 components and a later slow wave-like potential were not reduced, nor were their latencies altered. Clonidine produced increased EEG power in the alpha range (7.5–12 Hz) and decreased power in the upper beta range (20–40 Hz) but did not affect performance in the oddball task. Because two major effects of clonidine are to substantially reduce activity in the noradrenergic nucleus locus coeruleus (LC) and to reduce norepinephrine (NE) release from axons, the present results support the hypothesis that the LC and its efferent projection system are important in modulating the activity of P300-like potentials.