新生儿缺氧缺血性脑病血浆Th2 源细胞因子水平的研究

目的　研究新生儿缺氧缺血性脑病 (HIE)患儿辅助性T淋巴细胞亚群 2 (Th2 )细胞功能变化及其与HIE间的关系。　方法　采用ELISA法和单克隆抗体间接免疫荧光法 ,检测 4 6例HIE患儿不同病期血浆和外周血单个核细胞 (PBMC)体外分别产生白细胞介素 (IL) 6、IL 8、IL 10水平和T细胞IL 2受体 (IL 2R)表达率 ,并与正常足月新生儿脐血 (2 0例 )进行对照。　结果　HIE病程第 1天血浆及PBMC体外产生IL 6 [分别为 6 6 .3、80 4 .5ng L(中位数 ,以下同 ) ]、IL 8(4 10 .5、1983.6ng L)和IL 10 (2 7.9、14 0 .1ng L) ,明显高于正常脐血对照组 (分别为 1.9、187.7;91.0、76 6 .8;2 .6、16 .4ng L) (Z =4 .6 5 3～ 5 .85 6 ,P <0 .0 1) ;中重度组及并发多器官功能损害 (MOD)的HIE患儿血浆及PBMC体外产生IL 6 (分别为 86 .0、12 36 .4 ;111.3、174 6 .9ng L)、IL 8(分别为5 79.5、2 812 .2 ;5 32 .8、2 94 3.3ng L) ,分别高于轻度组 (分别为 5 2 .6、5 83.4 ;335 .3、15 2 8.9ng L)及无MOD组 (分别为 5 6 .6、713.8;35 2 .4、15 72 .4ng L)患儿 (Z =2 .2 2 8～ 2 .70 1,P <0 .0 5 ;Z =2 .877～4 .187,P <0 .0 1) ,而IL 10差异无显著性 (Z =0 .4 5 0～ 1.85 0 ,P >0 .0 5 ) ;T细胞IL 2R表达率 [(5 2 .8± 13.4 ) % ]明显     

Low vitamin D status is associated with low cord blood levels of the immunosuppressive cytokine interleukin-10

The cytokine interleukin-10 (IL-10) plays a pivotal regulatory role in tolerizing exogenous antigens. Experimental data indicate that low cellular availability of the vitamin D hormone 1,25-dihydroxyvitamin D [1,25(OH)2D] results in a down-regulation of IL-10 concentrations. The tissue production of an adequate amount of 1,25(OH)2D depends on a high circulating 25-hydroxyvitamin D (25-OHD) level. The present study was thus aimed at evaluating the associations between season of birth, vitamin D status, and the allergy risk markers IL-10 and total immunoglobulin (IgE) in newborns. Cord blood was obtained from 49 infants born during the summer half year (mid-April to mid-October, geographic latitude 51 degrees N) and from 47 infants born during the winter half year (mid-October to mid-April, geographic latitude of 51 degrees N). Serum levels of 25-OHD were 99% higher, and IL-10 levels were 43% higher in the summer half year compared with the winter half year (p < 0.001 and p = 0.018). Moreover, the ratio of IL-10 to total IgE was 124% higher in the summer half year compared with the winter half year (p = 0.039). Serum levels of 25-OHD were correlated with IL-10 levels (r = +0.22; p < 0.05). Mothers' age, gestational ages, birth weights and serum 1,25(OH)2D levels did not differ between study groups. We conclude that the low vitamin D status of infants born in winter may at least in part adversely affect biomarkers of allergy risk.     

Association between interleukin-10 genetic polymorphisms and risk of primary open angle glaucoma in a Chinese Han population: a case-control study

AIM: To investigate the association between interleukin-10 (IL-10) genetic polymorphisms and risk of POAG through a case-control study in a Han population of China. METHODS: A total of 210 patients with POAG and 420 normal subjects were recruited during the period from Dec. 2013 to Dec. 2016. The IL-10 -1082A>G (rs1800870), -819T>C (rs1800871) and -592C>A (rs1800872) polymorphisms were determined using iPlex GOLD SNP genotyping analysis (the SequenomMassARRAY® System, Sequenom, San Diego, USA). The association between IL-10 -1082A>G (rs1800870), -819T>C (rs1800871), and -592C>A (rs1800872) polymorphisms and risk of POAG was assessed by singlelogistic regression analysis. RESULTS: We observed that those carrying the CC genotype of rs1800871 was associated with an increased risk of POAG when compared with those harboring the TT genotype (OR=1.84, 95%CI=1.01-3.38). Those with AA genotype of rs1800872 had a 10.62 fold risk of POAG in comparison to the CC genotype (OR=10.62, 95%CI, 3.41-33.09). A completely linkage disequilibrium was found between IL-10 rs1800871-rs1800872 (D’=1.00, r2=0.16). The A-C-A (OR=2.60, 95%CI, 1.48-4.58) and G-T-A (OR=2.34, 95%CI, 1.42-3.86) haplotypes were associated with an increased risk of POAG, while the A-T-C haplotype showed a decreased risk of POAG (OR=0.63, 95%CI, 0.49-0.81). CONCLUSION: Our data suggest that IL-10 rs1800871 and rs1800872 can be predictive factors for the pathogenesis of POAG in the Chinese population.     

Increased Ifng and Il10 Expression Correlate with Disease in Rodent Models Experimentally Infected with Modoc Virus

Flaviviruses present an ongoing threat to global public health, although the factors that contribute to the disease remain incompletely understood. We examined an acute Modoc virus (MODV) infection of two rodent models. Viral RNA was detected in the kidneys, spleen, liver, brain, urine, and sera of experimentally infected deer mice, a reservoir host of MODV, and Syrian hamsters, a known disease model. As expected, clinical outcomes differed between species, and the levels of viral RNA recovered from various tissues demonstrated signs of differential replication and tissue tropism. Multivariate analysis indicated significance in the profile of expressed genes between species when analyzed across tissues and over time (p = 0.02). Between-subject effects with corrected models revealed a significance specific to the expression of Ifng (p = 0.01). the expression of Ifng was elevated in hamsters as compared to deer mice in brain tissues at all timepoints. As the over-expression of Ifng has been shown to correlate with decreased vascular integrity, the findings presented here offer a potential mechanism for viral dissemination into the CNS. The expression of IL10 also differed significantly between species at certain timepoints in brain tissues; however, it is uncertain how increased expression of this cytokine may influence the outcome of MODV-induced pathology.     

子宫内膜异位症患者腹腔液中IL-6、IL-8、IL-10含量升高及其临床意义

目的：检测子宫内膜异位症（endometriosis,EMS）患者腹腔液中IL-6、IL-8、IL-10含量及其临床意义。方法：剖腹探查手术中采集EMS组及对照组的腹腔液,用酶联免疫吸附法（ELISA）测细胞因子水平。EMS组中,13份测IL-6,16份测IL-8,22份测IL-10,共51份,对照组检测57份。结果：EMS组和对照组IL-6的含量分别为155．88±114．92ng／L和28．74±25．88ng／L（P＜0．01）。IL-8的含量分别为630．97±404．72ng／L和63．05±62．3ng／L（P＜0．01）。IL-10也明显高于对照组［（38．88±38．75ng／L比10．45±4．33ng／L（P＜0．01）〕。结论：EMS患者腹腔液中IL-6、IL-8及IL-10均升高,提示EMS患者的巨噬细胞活性增强,细胞因子含量增加,可干扰患者免疫调节功能。     

Astrocytes in the damaged brain: Molecular and cellular insights into their reactive response and healing potential

Long considered merely a trophic and mechanical support to neurons, astrocytes have progressively taken the center stage as their ability to react to acute and chronic neurodegenerative situations became increasingly clear. Reactive astrogliosis starts when trigger molecules produced at the injury site drive astrocytes to leave their quiescent state and become activated. Distinctive morphological and biochemical features characterize this process (cell hypertrophy, upregulation of intermediate filaments, and increased cell proliferation). Moreover, reactive astrocytes migrate towards the injured area to constitute the glial scar, and release factors mediating the tissue inflammatory response and remodeling after lesion. A novel view of astrogliosis derives from the finding that subsets of reactive astrocytes can recapitulate stem cell/progenitor features after damage, fostering the concept of astroglia as a promising target for reparative therapies. But which biochemical/signaling pathways modulate astrogliosis with respect to both the time after injury and the type of damage? Are reactive astrocytes overall beneficial or detrimental for neuroprotection and tissue regeneration? This debate has been animating this research field for several years now, and an integrated view on the results obtained and the possible future perspectives is needed. With this Commentary article we have attempted to answer the above-mentioned questions by reviewing the current knowledge on the molecular mechanisms controlling and sustaining the reaction of astroglia to injury and its stem cell-like properties. Moreover, the cellular/molecular mechanisms supporting the detrimental or beneficial features of astrogliosis have been scrutinized to gain insights on possible pharmacological approaches to enhance astrocyte neuroprotective activities.     

不同病因慢性肝病患者肝组织中IL-10的表达及意义

目的：探讨不同病因的慢性肝病（ CLD）患者肝组织中白细胞介素?10（ IL?10）的表达及其与肝组织炎症和纤维化的关系。方法收集93例CLD患者及23例正常对照肝组织石蜡标本,免疫组织化学方法检测肝组织中IL?10的表达。结果不同病因的CLD患者肝组织中肝小叶及汇管区IL?10的表达均较正常对照明显增高（ P＜0．01）。随着肝脏炎症分级（ G）的增高,CLD患者肝组织内肝小叶及汇管区IL?10的表达逐渐增强,差异有统计学意义。随着肝脏纤维化程度分期（ S）的增高,CLD患者肝组织内肝小叶及汇管区IL?10的表达亦逐渐增高,差异有统计学意义。结论 IL?10可能参与CLD患者肝脏的炎症和纤维化的发生。     

体外导入外源IL-10基因对HSC增殖的影响

目的利用重组复制缺陷型腺病毒AdIL 10将外源IL 10基因导入肝星状细胞 (HSC) ,并观察其对HSC增殖的影响。方法将本实验室自行构建的重组腺病毒质粒pAdIL 10用Lipofectamin包装转染HEK 2 93细胞 ,即可获得重组腺病毒AdIL 10。应用胶原酶原位灌注和密度梯度离心法分离大鼠HSC ,当HSC生长至 80 %密度时 ,以 5× 10 4细胞 /孔接种于 96孔板 ;HSC培养 2 4h后 ,取病毒AdIL 10以感染复数 (MOI) 5 0感染HSC ;用MTT法检测HSC增殖率。结果pAdIL 10经 2 93细胞包装 ,3d后可观察到绿色荧光蛋白 (GFP)表达 ;AdIL 10感染HSC 3d后可观察到GFP表达 ,且HSC增殖受到抑制。结论重组腺病毒AdIL 10感染HSC后可抑制其增殖     

保妇康栓对念珠菌阴道炎患者的局部免疫调节作用

目的:观察保妇康栓对念珠菌阴道炎患者阴道分泌物中HBD-2、IL-10表达的影响,探讨其对念珠菌阴道炎患者局部免疫调节的机制。方法:选择对照组10例。念珠菌阴道炎患者60例,随机分成两组,为保妇康栓治疗组及达克宁治疗组,观察治疗前后各组患者HBD-2、IL-10表达变化。结果:念珠菌阴道炎患者阴道分泌物中HBD-2、IL-10水平与正常对照组患者比较均有显著性差异(P<0.01)。保妇康栓治疗组及达克宁治疗组患者治疗前后两者比较差异有统计学意义(P<0.05)。结论:保妇康栓对念珠菌阴道炎患者阴道分泌物中HBD-2、IL-10表达有影响,其机制可能为免疫抑制。