全文获取类型
收费全文 | 152篇 |
免费 | 4篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 1篇 |
妇产科学 | 1篇 |
基础医学 | 20篇 |
口腔科学 | 5篇 |
临床医学 | 3篇 |
内科学 | 2篇 |
皮肤病学 | 41篇 |
神经病学 | 1篇 |
特种医学 | 7篇 |
外科学 | 51篇 |
综合类 | 9篇 |
眼科学 | 3篇 |
药学 | 10篇 |
中国医学 | 1篇 |
出版年
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 3篇 |
2015年 | 4篇 |
2014年 | 9篇 |
2013年 | 4篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 10篇 |
2008年 | 11篇 |
2007年 | 11篇 |
2006年 | 5篇 |
2005年 | 10篇 |
2004年 | 10篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有156条查询结果,搜索用时 0 毫秒
1.
2.
明胶降解物对大鼠真皮成纤维细胞增殖及Ⅰ型胶原蛋白分泌的影响 总被引:1,自引:0,他引:1
目的 探讨组织工程心瓣膜常用人工基质材料--明胶发生降解后的产物对大鼠真皮成纤维细胞增殖及Ⅰ型胶原蛋白分泌的影响. 方法 利用酶组合技术制备明胶降解物(gelatin hydrolysate, GH),采用超滤的方法分离出低分子量明胶降解物组分(gelatin hydrolysate fraction, GHF),用于5只SD大鼠真皮成纤维细胞的体外培养.采用CCK-8活细胞计数试剂盒(Cell Counting Kit-8)检测细胞的增殖情况,ELISA试剂盒检测细胞I型胶原蛋白的分泌情况. 结果 ①GHF对细胞增殖作用的浓度效应:在0.125~1.0 mg/ml浓度范围内,GHF对细胞的促增殖作用呈现先增强后减弱的趋势,在0.25 mg/ml时,细胞增殖率最大,达到(47.54±16.35)%;②GH与GHF对细胞增殖影响比较:GH与GHF均具有促进细胞增殖的作用,且在浓度为0.25 mg/ml时,促细胞增殖作用无显著差异[(27.04±15.21)% vs (39.22±15.55)%, P=0.177];③GH与GHF对细胞分泌Ⅰ型胶原蛋白影响比较:GHF组第3天时Ⅰ型胶原分泌量为(28.06±5.60)pg/105,与空白对照的(18.24±4.52)pg/105相比,有显著差异(P=0.006),而GH组直至第6天时Ⅰ型胶原蛋白分泌量,与空白对照相比,无统计学意义(P=0.103). 结论 分子量大小不同的明胶降解物GH与GHF均具有促进大鼠真皮成纤维细胞增殖的作用,且分子量较小的GHF还具有促进细胞I型胶原分泌的功能. 相似文献
3.
目的 探讨十二烷基硫酸钠制备脱细胞真皮基质的最佳浓度及作用时间.方法 取临床截肢患者废弃的皮肤,分成90块,随机分成6组,每块约1 cm×1 cm,分别置于0.1%、0.25%、0.5%、1.0%、2.5%、5.0%十二烷基硫酸钠溶液中脱细胞,每隔8 h取材一次,分别取材15次.取材标本行大体和组织学观察.结果 肉眼观察十二烷基硫酸钠作用8 h后,0.25%组可将表皮与真皮完整分离;光镜检查显示0.25%十二烷基硫酸钠在作用40 h后,能将真皮内的细胞及细胞碎片脱净,形成脱细胞真皮基质.制备的脱细胞真皮基质,主要由胶原纤维网架构成,其他组作用120 h也无法形成真正的脱细胞真皮基质.结论 十二烷基硫酸钠制备脱细胞真皮基质的最佳浓度为0.25%,最佳时间为40 h.与其他方法比较,具有简便、快捷、有效、价廉、宜推广等特点,可为临床急诊的修复争取宝贵的时间. 相似文献
4.
Summary An evaluation of the rheological properties of skin is provided by measuring in situ the ability of skin to withstand vertical forces of extension. In physiological conditions the biomechanical parameters are reproducible and related to age, sex, skin thickness and the microarchitecture of the connective tissue studied by scanning electron microscopy. These observations shed some light on the structure-function relationship of the fibrous framework of the connective tissue. Variation in the biomechanical parameters can be used to substantiate clinical diagnosis and to monitor the evolution and the therapy of various diseases of the connective tissue.Presented in part at the 3rd Meeting of the European Society for Dermatological Research, Amsterdam (1973) and at the Meeting on Measurement of Physical Properties of Skin, Miami (1976) 相似文献
5.
《Anais brasileiros de dermatologia》2020,95(2):247-249
Pseudoxanthoma elasticum-like papillary dermal elastolysis is an acquired elastic tissue disorder clinically similar to pseudoxanthoma elasticum in the absence of systemic involvement. Histopathologically, special staining of elastic fibers demonstrates a total or partial band-like loss of elastic fibers in the papillary dermis. Although ultraviolet radiation seems to be one of the main etiological factors in this entity, we report a case of pseudoxanthoma elasticum-like papillary dermal elastolysis on the neck of a woman who wore hijab. 相似文献
6.
Mubashir Younis Ajaz Ahmed Shah Shahid Hassan Muneet Kapoor Abina Rashid 《Journal of maxillofacial and oral surgery》2021,20(1):54
IntroductionTemporomandibular joint (TMJ) ankylosis is an extremely disabling condition with almost complete inability to open the jaws causing difficulty in chewing, speech, poor oral hygiene and cosmetic disfigurement. Temporalis myofascial flap still remains the most common interpositional material used; however, patients usually complain of pain during movement, unesthetic bulging in the temporal region and trismus due to scar contracture. The main aim of the study was to evaluate the efficacy of abdominal dermis-fat graft and compare it with temporalis myofascial flap as to see which of the two grafts offers more advantages and provides better postoperative results following TMJ ankylosis surgery. Materials and MethodsA total of 30 diagnosed cases of TMJ ankylosis were randomly divided into two groups of 15 patients each. All the patients underwent TMJ ankylosis release under general anesthesia followed by abdominal dermis-fat interposition in Group A and temporalis muscle in Group B. The patients were assessed for pre-operative and postoperative mouth opening (immediate and 6 month postoperative), pain during physiotherapy, donor and surgical site complications and recurrence of ankylosis.ResultsThe mean maximum inter-incisal opening in dermis-fat group was significantly higher than temporalis group both at immediate and 6 month postoperative periods (p = 0.041, 0.001). Physiotherapy was less painful in dermis-fat group than in temporalis group, and the differences in VAS scores among the 2 groups showed high statistical significance (p < 0.001). Hypertrophic scar developed at the donor site in 2 patients in dermis-fat group; however, it was located below the beltline and hardly noticeable. A total of 9 patients (4 in Group A and 5 in Group B) developed temporary facial nerve weakness, and no case of re-ankylosis was noted in either group.ConclusionDermis-fat graft in temporomandibular joint ankylosis showed better results than conventional temporalis myofascial flap in terms of postoperative mouth opening, physiotherapy and jaw function with esthetically acceptable results. 相似文献
7.
Fumihiro Mizokami Katsunori Furuta Atsushi Utani Zenzo Isogai 《International wound journal》2013,10(5):606-611
A pressure ulcer is a localised injury of the skin and underlying tissue that usually develops over a bony prominence. A decrease in the pressure over the bony prominence effectively prevents pressure ulcers; however, no studies have systematically assessed the physical properties of existing pressure ulcers. To characterise pressure ulcers, we established new terminology that clarifies the physical properties of pressure ulcers: wound mobility was defined as movement using the bony prominence as a predefined specific marker, and wound deformity was defined as a change in the three‐dimensional shape of the wound. Observational studies using this terminology showed that the distinct physical properties of pressure ulcers depend on the site of development and the wound depth according to the National Pressure Ulcer Advisory Panel criteria. Most grade IV sacrum pressure ulcers exhibited mobility and deformity. Superficial sacrum pressure ulcers below grade II showed only mobility. In contrast, foot pressure ulcers did not exhibit mobility or deformity. We propose a new concept, ‘wound physical property’, for understanding the unique pathogenesis of pressure ulcers. 相似文献
8.
Lior Rosenberg Yuval Krieger Alex Bogdanov-Berezovski Eldad Silberstein Yaron Shoham Adam J. Singer 《Burns : journal of the International Society for Burn Injuries》2014
Objectives
Excisional debridement followed by autografting is the standard of care (SOC) for deep burns, but is associated with serious potential complications. Conservative, non-surgical and current enzymatic debridement methods are inefficiently slow. We determined whether a non-surgical option of rapid enzymatic debridement with the debriding enzyme NexoBrid™ (NXB) would reduce need for surgery while achieving similar esthetic and functional outcomes as SOC.Methods
We conducted a multi-center, open-label, randomized, controlled clinical trial including patients aged 4-55 years with deep partial and full thickness burns covering 5-30% of their total body surface area (TBSA). Patients were randomly assigned to burn debridement with NXB (applied for 4 h) or SOC, which included surgical excisional or non-surgical debridement.Results
NXB significantly reduced the time from injury to complete débridement (2.2 vs. 8.7 days, P < 0.0001), need for surgery (24.5% vs. 70.0%, P < 0.0001), the area of burns excised (13.1% vs. 56.7%, P < 0.0001) and the need for autografting (17.9% vs. 34.1%, P = 0.01). Scar quality and quality of life scores were similar in both study groups as were the rates of adverse events.Conclusions
Enzymatic débridement with NXB resulted in reduced need for and extent of surgery compared with SOC while achieving comparable long-term results in patients with deep burns.Trial registration
: Clinical Trials.gov NCT00324311. 相似文献9.
真皮干细胞具有增殖和多向分化的潜能,角质形成细胞通过表达Wnt3a和E钙黏素来诱导真皮干细胞移行和分化为黑素细胞.研究表明,在黑素细胞形成过程中,真皮黑素细胞与表皮黑素细胞依赖的信号通路明显不同,表皮黑素细胞高度依赖干细胞因子信号通路,而真皮黑素细胞高度依赖内皮素3和肝细胞生长因子信号通路.目前关于色素痣痣细胞的来源尚不清楚,有研究认为,痣细胞来源于能分化为黑素细胞的干细胞,真皮干细胞能分化为黑素细胞,是否是痣细胞的来源尚需进一步研究. 相似文献
10.
目的 探讨真皮模板单元-黏着斑微操控制对皮肤成纤维细胞生物学行为影响. 方法 采用微图案方法制作微凸阵列,运用分子自组装在微凸上自组装结构蛋白分子,在自制细胞培养仪模拟皮肤生物力学进行细胞牵拉2周,并行形态学观察和细胞免疫组织化学分析. 结果 细胞牵拉2周后,细胞形态多样、丰满,分泌增加,呈现顺应性.细胞免疫组织化学分析发现,整合素β1、α5表达较对照显著减少,张力蛋白tensin表达显著降低. 结论 通过黏着斑微操控,增生性瘢痕成纤维细胞在形态学及生物学行为上发生改变. 相似文献