基于IgG抗A（B）效价梯度及动态差值探讨ABO-HDN病情相关性

目的探讨动态监测IgG抗A(B)效价梯度与ABO-HDN病情严重程度临床指标的相关性。方法选取2017年1~5月间47例ABO溶血病患儿临床资料进行统计分析,记录患儿出生后32 h的血清总胆红素(serum total bilirubin,STB_(32h))、血红蛋白(hemoglobin,Hb)、网织红细胞百分比(the percentage of reticulocyte,Ret%)及孕妇第16和37周IgG抗A(B)效价梯度。比较各组新生儿临床指标和lgG抗A(B)效价的相关性。结果 STB_(32h)与37周IgG抗A(B)效价梯度、IgG抗A(B)_(37-16)梯度差呈正相关(P0.001);Hb与第37周IgG抗A(B)效价梯度呈负相关(r~2=0.2074,P=0.0013);Hb与IgG抗A(B)_(37-16)梯度差值呈负相关(r~2=0.1831;P=0.0027);Ret%与37周IgG抗A(B)效价梯度、IgG抗A(B)_(37-16)梯度差值呈正相关(P0.001)。结论产前37周IgG抗A(B)效价梯度、IgG抗A(B)_(37-16)效价梯度差值与新生儿ABO-HDN的病情严重程度具有一定相关性。     

Presence of Hepatitis E Virus in a RED Deer (Cervus elaphus) Population in Central Italy

Hepatitis E is an acute human disease caused by the hepatitis E virus (HEV). In addition to humans, HEV has been detected in several animal species and is recognized as a zoonotic pathogen. Pigs, wild boar and deer can be reservoir. In this study, we evaluated HEV prevalence in a free‐living red deer (Cervus elaphus) population in central Italy by detecting virus‐specific antibodies and RNA in sera. A total of 35 of 251 red deer sera were positive for anti‐HEV IgG. HEV RNA was detected in 10 of 91 sera examined. Two genomic fragments targeted by diagnostic PCRs in the capsid region were sequenced, both matching with genotype 3 HEV. Overall results confirmed the occurrence of HEV infection in deer also in Italy.     

IgG4-related disease

The term “IgG4-related disease” encompasses several disorders described many years ago under various designations depending on the organ or system involved (e.g., Mikulicz syndrome, Riedel's thyroiditis, and retroperitoneal fibrosis). The clinical presentation varies widely, as one or more organs may be affected, usually in the same region of the body and either synchronously or metachronously. The main targets are the pancreas, bile ducts, salivary glands, lachrymal glands, mediastinal lymph nodes, and retroperitoneum. IgG4-related disease is rare, with an estimated incidence of 0.2 to 1/100 000 in Japan and no available incidence data in occidental countries. Men older than 50 years are predominantly affected. Serum IgG4 levels are often greater than 1.35 g/L. Enlargement of the involved organs, which may be pseudotumoral, is due to a combination of infiltration by T cells and IgG4-expressing plasma cells, storiform fibrosis, and obliterative thrombophlebitis. Glucocorticoid therapy is effective but may be followed by relapses requiring the use of immunomodulating agents such as azathioprine, methotrexate and, more recently, rituximab. IgG4-related disease is not an autoimmune condition related to IgG4 autoantibodies, and neither does it involve immune complexes. Specific joint involvement has been reported in a very small number of patients.     

黑龙江省2019—2021年肾综合征出血热患者血清标本抗体检测

目的 检测黑龙江省2019—2021年肾综合征出血热(hemorrhagic fever with renal syndrome, HFRS)疑似患者血清标本中的汉坦病毒急性期和恢复期抗体水平,为该疾病防控提供科学依据。方法 应用酶联免疫吸附法对采集到的肾综合征出血热疑似患者急性期血清样本进行汉坦病毒IgM抗体检测,对恢复期血清标本进行IgM单抗体、IgG单抗体检测,使用SPSS 19.0软件对各年份间患者急性期血样IgM抗体阳性率进行χ²检验分析,使用EpiData 3.1和Excel2003软件对患者性别、职业、年龄、发病日期及初诊间隔时间数据进行整理分析。结果 共检测肾综合征出血热疑似患者急性期血清351份,恢复期血清208份。急性期血清标本IgM抗体阳性317份,阳性率为90.31%,各年份之间患者急性期IgM抗体阳性率差异无统计学意义(χ²=0.895,P=0.639)。患者恢复期血清IgM单抗体阳性32份(15.39%);血清IgG单抗体阳性28份(13.46%);IgM、IgG抗体双阳性148份(71.15%)。男女患者性别比4...     

睾丸性无精子症中医证型间睾丸病理类型、IgG、IgM的比较研究

目的 通过对睾丸性无精子症肾虚证组、血瘀证组患者睾丸病理类型、IgG、IgM、的比较研究，为辨证论治睾丸性无精子症提供理论依据。方法 31例睾丸性无精子症患者的睾丸组织，其中。肾虚证者16例，血瘀证者15例。正常对照组睾丸5例。进行病理检查，睾丸病变程度行Makler评分，利用免疫组织化学ABC法检测睾丸组织中IgG、IgM沉积情况，并对上述结果行统计学处理。结果两组患者的病理类型都以生精阻滞为主，但血瘀证组患者的睾丸病变更为严重而复杂。31例患者中22例有IgG沉积，11例有IgM沉积，两组间比较无显著性差异，免疫复合物的沉积与睾丸病变程度无关。结论血瘀证患者睾丸组织病变更为严重。自身免疫在肾虚证、血瘀证患者中普遍存在，但免疫因素在睾丸性无精症的发病机制中不占主导地位，自身免疫与证型无关。     

IgG4相关肾病的CT影像特征

目的探讨IgG4相关肾病CT影像特点。方法回顾性分析36例IgG4相关肾病患者,其中累及肾实质26例,累及肾盂10例,双肾发病或多病灶24例,单病灶12例,分析其影像学特征,包括临床病史、病灶的大小、形态、密度、分布范围、增强后强化的特点、峰值、肾盂受累情况、有无淋巴结肿大等。结果 IgG4相关肾病具有较特异的临床及影像学特征,虽然肾脏或肾盂受累,但无肾积水及血尿等恶性肿瘤常见临床表现,边界多清楚完整,其具有典型的持续性渐进性强化的特点,峰值多位于实质期及排泄期,病变无液化、坏死、囊变及钙化等影像学表现,即使肾盂受累明显而且病灶较大也无周围肾实质侵犯,肾盂形态依然存在,管壁光整,无周围淋巴结增大。结论 IgG4相关肾病CT影像与肾脏恶性肿瘤相似,结合其临床、CT影像和血IgG4相关实验室检查,可以做出明确诊断,避免不必要手术。     

3类新型慢性唾液腺炎的诊断和治疗

慢性唾液腺炎是组织病理学以唾液腺慢性炎症为主要表现的一类疾病,有传统的已经熟知的慢性唾液腺炎,也有新近才被认识或者受到重视的慢性唾液腺炎,即新型慢性唾液腺炎;有唾液腺本身局部病变为主的疾病,也有唾液腺作为靶器官受损的全身性疾病.传统的已经熟知的慢性唾液腺炎有慢性复发性腮腺炎、唾液腺结石伴发的唾液腺炎等.唾液腺内镜的引入使其治疗发生根本变化,尽可能保留唾液腺功能器官的理念正在进一步深化[1-3].     

自身免疫性垂体炎发病机制的研究进展

自身免疫性垂体炎(AH)是一种罕见的垂体炎性疾病。其临床表现主要以垂体内分泌异常或炎症压迫为特征,典型症状为头痛、视觉受压、多饮、多尿等,并可伴有其他自身免疫性疾病。尽管进行了大量研究,但AH的发病机制尚未完全阐明。因此本文讨论了最近关于自身免疫性垂体炎的潜在机制的发现,以深刻理解AH发生发展的病理生理过程,并可能为AH的诊断和治疗提供新的方向。     

黑线毛足鼠和金仓鼠血清IgG的纯化及抗血清的制备

目的纯化黑线毛足鼠和金仓鼠血清IgG,制备兔抗金仓鼠和黑线毛足鼠血清IgG的抗血清。方法用Hitrap Protein G亲和层析纯化黑线毛足鼠和金仓鼠血清IgG,经SDS-PAGE电泳鉴定纯度,标准免疫方法免疫兔子制备抗血清。结果黑线毛足鼠和金仓鼠血清IgG对protein G有很高的亲合性,用Hitrap Protein G亲和层析纯化,得到高纯度黑线毛足鼠和金仓鼠IgG,利用纯化的IgG作抗原制备了高效价的抗血清,免疫双扩散测定效价达1∶32和1∶16。结论证实黑线毛足鼠和金仓鼠IgG和Protein G具有很高的亲和性,Protein G亲和层析是纯化黑线毛足鼠和金仓鼠IgG有效的方法之一,制备了黑线毛足鼠和金仓鼠IgG的抗血清。     

92例孕妇产前IgG抗体效价的微柱凝胶法检测

目的探讨微柱凝胶法检测对孕妇产前IgG抗体效价的意义。方法取92例夫妻血型不合的O型孕妇血清进行微柱凝胶法检测,并观察新生儿ABO新生儿溶血病(HDN)的发病情况。结果IgG抗A抗体效价≥64者17例,占39.53%;抗B抗体效价≥64者20例,占40.82%。IgG抗A抗体者的总发病率为12.20%,IgG抗B抗体者的总发病率为14.29%。2者的发病均出现在抗体效价≥64。结论产前检测IgG抗体水平具有重要的临床意义。