MEBO对深Ⅱ度烧伤胶原ⅠⅢ Ⅳ型变化的研究

目的：以ＭＥＢＯ治疗深Ⅱ度烧伤观察创面愈合前后Ⅰ,Ⅱ,Ⅳ型胶原变化。探讨ＭＥＢＯ与烧伤疤痕间关系。方法：设治疗组与传统干性治疗组各１５例并切取治疗前后活体组织用免疫组化ＳＰ染色法观察治疗前后胶原Ⅰ,Ⅲ,Ⅳ型变化。结果：ＭＥＢＯ治疗组Ⅰ型含量（＋）－（＋＋）１２／１５,（（＋＋＋）３／０,Ⅲ型（＋）－（＋＋）１５／６）,（＋＋＋）０／９,Ⅳ型（＋）－（＋＋）１５／３,（＋＋＋）０／１２。     

用乙酰胆碱酯酶的组织化学定位法对怡乐村并殖吸虫神经系统的观察

应用乙酰胆碱酯酶定位技术对怡乐村并殖吸虫后尾蚴、不同发育时期的童虫及成虫神经系统进行整体染色观察。结果表明:后尾蚴体内仅具有两个孤立的神经节。而童虫和成虫体内神经系统的发育顺序为:两个中枢神经节由粗大的神经连合连接,并向后伸出一对后腹神经干,向前伸出二对前腹和咽神经干,向后伸出后侧和后背二对神经干,从中枢神经节向前伸出二对前背和前侧神经干与前腹神经干汇合于口吸盘处形成神经网,从后腹神经干也伸出分支入腹吸盘,在后行的3对神经干中,以后腹神经干最粗大,并在体后1/5处汇合,另二对则在体后部逐渐变细消失。在后3对神经干间,有横向神经纤维相连。在腹侧,横向纤维间有纵行分支伸出相连,构成神经网。     

Computer‐assisted morphometric analysis of lymphatic vessel changes in hamster tongue carcinogenesis

J Oral Pathol Med (2010) 39 : 518–524 Background: To characterize lymphangiogenesis in early‐stage hamster tongue carcinoma development, morphological features and spatial relationships of lymphatic vessels. Methods: Lymphatic vessels were examined histochemically, using 5′‐Nase‐ALPase enzyme and combined light and electron microscopy to measure lymphatic vessel area (LVA) and lymphatic vessel density (LVD). Results: In atypical hyperplastic tissues, LVA was found to be 1429.97 and LVD was found to be 39, in carcinoma in situ LVA was 2538.33 and LVD was 48, and in micro‐invasive carcinoma LVA was 5733.74 and LVD was 59. Increased lymphangiogenesis was seen in pre‐neoplastic states and in early‐stage oral squamous cell carcinoma (OSCC). Small regular lymphatic vessels predominated in atypical hyperplasia, and large, irregular lymphatic vessels in early‐stage OSCC. Lymphatic endothelial vessels were stretched and porous over large areas. Conclusions: Newly formed lymphatics and patulous intercellular junctions may be optimally suited for tumor cell metastasis through lymphatic channels in early‐ and middle‐phase carcinogenesis. Lymphatic capillary LVA and LVD became enlarged, and positively correlated, with malignancy, but show no correlation with 7,12‐dimethylbenz[a]anthracene‐induced time.     

咬合垂直距离降低后大鼠咬肌的酶组织化学定量研究

目的：观察咬合垂直距离降低后大鼠咬肌酶组织化学特征的改变。方法：将40只雄性SD大鼠随机平均分为实验组与操作对照组,采用人为降低牙冠高度的方法降低实验组大鼠咬合垂直距离,实验开始3、10、30、60d时处死动物,取深层咬肌行辅酶Ⅰ-四氮唑还原酶（NADH-TR）染色,借助显微图像分析系统计算分析各型肌纤维的平均横截面积及百分构成比变化。结果：（1）大鼠深层咬肌由Ⅱa与Ⅱb两种肌纤维构成;（2）实验60天组大鼠Ⅱa型肌纤维平均截面积（mCAS-2a）明显小于实验3天组和同期操作对照组（P〈0.05）;（3）实验60天组Ⅱb型肌纤维平均截面积（mCAS-2b）明显大于实验3天组和同期操作对照组（P〈0.05）;（4）实验60天组Ⅱb型肌纤维所占百分构成比明显高于实验3天组与同期操作对照组（P〈0.05）。结论：咬合垂直距离降低后大鼠咬肌发生了形态结构的功能适应性改建。     

五种凝集素受体在膀胱癌中的分布及意义

目的 探讨膀胱癌中凝集素受体的分布与其分化程度、浸润深度、转移和预后的关系。方法 应用生物素标记的茶生凝集素（ＰＮＡ）、麦胚凝集素（ＷＧＡ）、扁豆凝集素（ＬＣＡ）、刀豆凝集素（ＣｏｎＡ）及菜豆凝集素（ＰＨＡ?等５种凝集素对５２例人体膀胱癌,１０例正常人体膀胱粘中膜进行亲合组织化学法研究。结果 发现正常膀胱粘膜ＰＮＡ、ＷＧＡ及ＰＨＡ受体阴性,ＰＮＡ受体阳性率随膀胱癌病理分级的上升而递增,相互之间差异     

亲和组化法检测乳腺癌雌,孕激素受体状态的研究

用亲和组化法（半定量分析法）同时检测６７例乳腺癌和良性病变组织印片和石腊切片的ＥＲ／ＰｇＲ状态，二种玻片的ＥＲ阳性、阴性和总符合率分别为８９．５％、９６．６％和９２．５％；双顶阳性（ＥＲ＋／ＰｇＲ＋）、双项阴性（ＥＲ－／ＰｇＲ－）和双项总符合率分别为７５．９％、９２．６％和８２．１％；同种方法检测１５７例随机乳腺癌石腊切片，发现ＥＲ阳性率为５８．６％（９２例）；ＰｇＲ阳性率４８．４％（７６例）．１５７例随访资料提示，受体双项阳性者，其健康良好。乳腺癌分期同ＥＲ／ＰｇＲ状态的比较，ＥＲ＋／ＰｓＲ＋者的Ⅰ、Ⅱ和Ⅲ期病例分别为３７．５％、４５．３％和１７．２％，无Ⅳ期病例：而ＥＲ－／ＰｇＲ－者则为１３．８％、２２．４％和４３．１％．提示，用亲和组化法测定乳腺癌石腊切片ＥＲ／ＰｇＲ状态有较好的可信性。但对乳腺粘液腺癌和派杰氏病宜用切片或针吸涂片。     

Cellular localization of adenylate cyclase in the developing and mature inner ear of the mouse

The localization of adenylate cyclase in the developing and mature inner ear of the CBA/CBA mouse, in Shaker-1 and Shaker-2 mutants and in organ culture was determined by a histochemical procedure with adenylyl imidodiphosphate as substrate and Sr²⁺ as capture ion. Enzymatic activity was associated with cell membranes in stria vascularis and Reissner's membrane but could not be demonstrated in other cochlear structures. In stria vascularis, adenylate cyclase was found on the contraluminal infoldings of marginal cells but not on their luminal surface, while Reissner's membrane showed activity on its endolymphatic surface. Activity was also associated with utricular dark cells. Essentially the same localization but differing in quantitative aspects was observed during development, in organ culture and in the mutant strains. The speculative role of adenylate cyclase in cochlear ion and fluid balance is discussed.     

乳腺癌组织内唾液酸的表达及其与预后的关系

目的：检测１０４例乳腺癌组织内唾液酸的表达。方法：采用凝集素组织化学染色技术。结果：生物素化ＬＰＡ（Ｌｉｍｕｌｕｓｐｏｌｙｐｈｅｍｕｓａｇｇｌｕｔｉｎｉｎ）阳性率为５６．７％（５９／１０４）;唾液酸的阳性表达与临床病理分期及淋巴结转移密切相关;在１００例行根治性手术的乳腺癌中,４５例ＬＰＡ阴性患者的无瘤生存率明显高于５５例ＬＰＡ阳性患者（Ｐ＜０．０５）。结论：乳腺癌组织中,唾液酸的表达能够反映乳腺癌的恶性潜能,阳性表达是一项预后差的指标,生物素化ＬＰＡ为判断乳腺癌预后提供了一种方便可靠的新方法。     

大肠癌及癌旁粘膜上皮细胞核DNA含量的研究

应用组化和显微分光光度技术观察,结果表明正常大肠粘膜无论从组织学还是从组织化学上看来均为近二倍体(2 N),其核DNA含量变异甚小,但癌细胞核内DNA含量则明显高于正常和癌旁粘膜。高、中分化大肠腺癌之间的DNA含量存在着统计学上的差异,提示DNA含量与肿瘤的分化有关。在移行粘膜中可见典型增生和异常粘液分泌,与正常粘膜相比,它的二倍体细胞较少而三、四倍体细胞较多,有少数细胞核DNA含量超过四倍体,提示有与其细胞增生周期不一致的异常增生存在。鉴于移行粘膜不典型增生程度与DNA含量之间所存在的联系,作者等支持大肠的移行粘膜可能是一种癌前期变化的观点。     

卵巢粘液性肿瘤组化和免疫组化研究

对50例卵巢粘液性肿瘤进行组化和免疫组化研究。良性、交界性和恶性3组肿瘤酸性粘蛋白的含量和粘蛋白的分布范围形成鲜明对比;在甲苯胺蓝异染性方面有明显差异。这对鉴别卵巢粘液性肿瘤良、恶性有参考价值。3组肿瘤免疫组化阳性反应的程度无明显差异,腺癌分化的程度与CEA细胞阳性率不呈正相关。表明CEA染色不能判断卵巢粘液性肿瘤的恶性程度。正常卵巢组织CEA阴性,如果良性肿瘤内CEA含量较多,从生物化学上已暗示可能己越出良性范畴,但在组织学上尚未表现出来。应注意随访。