生长激素对胰腺癌细胞株增殖活性的调控

目的探讨生长激素对胰腺癌细胞株Bxpc-3增殖活性的影响。方法MTT法检测生长激素对胰腺癌细胞株Bxpc-3增殖活性,Tunel法标记凋亡阳性细胞、免疫组化法标记增殖细胞,激光共聚焦显微镜计数免疫荧光双标Tunel阳性细胞和增殖细胞。结果10-7mol/L、10-8mol/L、10-9mol/L浓度的生长激素在第2天明显的抑制胰腺癌细胞生长,伴随作用时间的延长这种抑制作用逐渐减轻,且出现了加速胰腺癌细胞生长的作用,第4天、第5天胰腺癌细胞的生长同正常对照组相比有显著差异(P<0.01)。结论本实验研究从细胞学的角度证实生长激素在开始阶段具有抑制胰腺癌细胞生长的作用,但是这种作用伴随时间的延长逐渐消失,且出现加速了肿瘤细胞的生长的现象,为临床肿瘤病人应用生长激素提供了一定的理论依据。     

内皮素在重症急性胰腺炎大鼠脑损伤发病过程的影响

目的:观察重症急性胰腺炎(SAP)大鼠血浆和脑组织内皮素-1(ET-1)水平与脑损伤的相关关系,以及生长抑素和生长激素的保护作用。方法:经胰胆管逆行注射3.5%牛磺胆酸钠2.5ml/kg建立大鼠SAP模型。将200只大鼠随机分成5组:重症急性胰腺炎组(SAP组)、正常对照组(N组)、生长抑素组(S组)、生长激素组(G组)和联合治疗组(S G组)。观察各组治疗前后脑水肿、血脑屏障、脑细胞凋亡指数、脑组织ET-1的免疫组化。结果:SAP大鼠体内血ET-1显著升高,脑水肿、血脑屏障损伤程度加重。免疫组化显示SAP大鼠脑组织ET-1的表达增强。生长激素和生长抑素可以降低血ET-1、NO水平,减轻脑组织水肿,减弱脑组织ET-1的表达,改善血脑屏障的通透性。同时观察到SAP大鼠时脑电图呈慢波改变,治疗后好转。结论:生长激素和生长抑素能降低SAP体内血浆和脑组织中的ET-1水平,改善脑水肿和血脑屏障的通透性,对SAP时脑损伤有保护作用。     

生长激素对血管瘤内皮细胞生长的影响

目的：研究生长激素对血管瘤发生发展的关系。方法：用细胞培养的方法，检测生长激素（GH），血管内皮生长因子（VEGF）、胰岛素生长因子1（IGF－1）对血管瘤内皮细胞是否具有直接的增殖作用，用流式细胞仪检测血管瘤内皮细胞表面是否表达生长激素受体；用免疫组织化学的方法检测增殖期血管瘤标本中是否表达生长激素受体。结果：生长激素没有明显促进血管瘤内皮细胞增殖，VEGF及IGF－1能直接促进血管瘤内皮细胞增殖，并且，在促进Py-4-1细胞增殖方面VEGF与IGF－1间具有协同作用，生长激素受体在血管瘤内皮细胞及增殖期血管瘤标本中无明显表达。结论：生长激素对血管瘤的发生发展无直接的生理作用。但还不能否定其间的生理作用。     

Cholinergic and glutamergic receptor functional regulation in long-term,low dose somatotropin and insulin treatment to ageing rats: Rejuvenation of brain function

The role of somatotropin and insulin treatment in the regulation of neurotransmitter levels in the ageing brain is not fully established. We evaluated the long-term, low dose effects of somatotropin and insulin on acetylcholine and glutamate receptor subtypes functional regulation in the cerebral cortex of young (4–16 weeks) and old rats (60–90 weeks). Somatotropin and insulin treated young rats showed significant upregulation in muscarinic M1 and M3 expression whereas in old rats, somatotropin and insulin treatment downregulated M1 and M3 expression. N-methyl-d-aspartate and metabotropic glutamate receptor gene expression were significantly downregulated with somatotropin treatment while insulin treatment showed upregulation in both young and old rats. Acetylcholine esterase activity showed a decrease with age and after somatotropin and insulin treatment, the activity increased in both young and old rats. Electroencephalogram studies confirmed the brain wave activity in both young and old somatotropin and insulin treated rats. The results highlight long-term low dose somatotropin and insulin treatment in regulating cholinergic and glutamergic receptors subtypes in ageing rats and rejuvenation of brain function.     

生长激素对小儿术后蛋白质代谢的影响

本研究选需静脉营养(PN)组:60kcal·kg~(-1)·d~(-1)大手术患儿22例;生长激素(GH)组,0.2IU·kg~(-1)·d~(-1)12例;对照组10例.结果:氮平衡术后GH组明显优于对照组;蛋白质动力学观察,总体蛋白质合成率GH组较对照组明显增高;快速反应蛋白(纤维蛋白、前白蛋白、转轶蛋白)术前二组无差异,术后GH组明显较对照组增高.总之,GH在小儿术后低热卡PN中发挥了明显氮保留和促蛋白质合成作用.     

Contribution of hGH20K variant to blood hGH response in sauna and exercise

Summary Exercise-induced increases in blood somatotropin (hGH) have always been considered in terms of quantity of the circulating molecules. Knowing that the hypophysis can release several GH species, we investigated the differential release in blood of total hGH (hGH_T) and the main hGH variant (hGH_20K) molecules in six trained male swimmers exposed to three different conditions known to favor GH release in blood: 45 min – 70% maximum oxygen uptake bicycling and swimming, and 20 min of sauna bathing. Based on the binding specificity of hGH antibodies, hGH_20K was isolated then assayed using the Nichols immunoradiometric assay system. All three experimental conditions produced significant (P < 0.001) elevations in blood hGHT and hGH_20K. In all three cases, mean blood hGH_20K contribution to blood hGH_T was relatively constant (11.9, SE 0.7%). Rises in rectal temperature were not statistically related to the changes in blood hGH_T. This demonstration of a relatively constant elevation in hGH_20K during bicycling, swimming, and sauna bathing can hardly explain the large differences in blood hGH_T responses reported in literature under similar conditions.     

Pathophysiology of gastrointestinal hormones

Gastrointestinal hormones (GI hormones) have received growing interest in endocrinology, gastroenterology and neuroendocrinology. Because of new methodological techniques, they can be measured in plasma and therefore be related to different pathophysiological conditions. In childhood, our present knowledge is as yet limited to the physiological rôle of gastrin at different ages and in some diseases (gastrinoma; Verner-Morrison syndrome) caused by humoral dysfunction. The present review relates the clinical important GI hormones to chemically classified families. The diagnostic value of determining endogenous hormone concentration in plasma and the validity of function tests carried out by administration of exogenous hormones are pointed out. Particular emphasis is given to the trophic action of GI hormones in the development and function of the gastrointestinal tract during childhood. More speculatively, GI hormones are involved in the complex function of the central nervous system, thus making food intake a trophotrophic action in a broader sense. Curriculum vitae. Ivo Henrichs was born 1946 in Kleve/Lower Rhine (FRG). After studying in Münster and by a stipend in Zurich, he graduated 1971 from Medical School of Münster and took his degree 1972 from the Medical Faculty of Zurich. He obtained his clinical training in the Center of Paediatrics, University of Ulm, with emphasis on pediatric endocrinology. His interest of research is the rôle of gastrointestinal hormones in childhood. Since 1979, after he was trained in the laboratory of Prof. K. D. Buchanan (Belfast), he is the recipient of a stipend from the German Research Council.Supported by a stipend from the Deutsche Forschungsgemeinschaft, He 1107/1     

重组人生长激素治疗腹腔感染后低白蛋白血症的实验与临床研究

目的探讨重组人生长激素（ｒｈＧＨ）对腹腔感染引起低白蛋白血症的治疗作用。方法应用逆转录聚合酶链反应（ＲＴＰＣＲ）方法,分别在体外实验、动物实验和临床研究中,观察ｒｈＧＨ对白蛋白ｍＲＮＡ表达和白蛋白合成的影响。结果ｒｈＧＨ明显促进体外培养肝细胞白蛋白基因表达（１４３％±６％,对照１００４％±２２％,Ｐ＜００１）;在内毒素损伤的肝细胞模型中,ｒｈＧＨ显著减轻内毒素介导的白蛋白ｍＲＮＡ表达抑制;在体内ｒｈＧＨ明显促进正常大鼠白蛋白ｍＲＮＡ表达（１５２％±８％,对照组１００％±３％,Ｐ＜００１）;促进腹腔感染大鼠白蛋白合成（２０４±１７ｇ／Ｌ,对照１５５±２９ｇ／Ｌ,Ｐ＜００１）;ｒｈＧＨ明显提高腹腔感染病人白蛋白、前白蛋白、转铁蛋白血清浓度。结论ｒｈＧＨ对腹腔感染引起的低白蛋白血症有明显的治疗作用。     

缺锌大鼠烫伤后补锌对血清和组织锌、碱性磷酸酶及生长激素的影响

目的 观察缺锌大鼠烫伤后补锌对体内锌、含锌酶、激素的影响。方法 用4ug/g低锌饲料大鼠1周,造成缺锌状态后15%深Ⅱ度烫伤,分3组进食不同含锌量饲料,L组（缺锌组）、M组（低补锌组）、H组（高补锌组）。伤后1、3、7d,分别活杀各组大鼠,留取血、组织标本。结果 血清锌,L、M组下降,H组上升,肝脏锌,各组均呈上升趋势,以H组最明显,骨骼锌：L组进行性下降。M组缓慢上升,H组上升最明显。烫伤皮肤锌：各组均呈上升趋势,以H组最明显,碱性磷酸酶（ALP）：L组明显降低,H组高于L、M组。生长激素（GH）：L、M组烫伤后第1天下降,而后各组逐渐升高,以H组最明显。结论 低锌状态下烫伤进一步加重机体缺锌,补锌可以纠正血清锌降低,增加肝脏、骨骼、皮肤含锌量;增加ALP活性,提高GH水平。     

饮食诱导肥胖抵抗大鼠激素敏感脂肪酶基因的表达

目的　探讨高脂饮食诱导肥胖抵抗 (DIO R)大鼠激素敏感脂肪酶基因的表达。方法5 0只健康雄性SD大鼠 ,随机分为对照组和高脂组 ,分别用基础饲料和高脂饲料喂养 13周 ,然后根据体重和能量摄入量筛选出DIO R和饮食诱导肥胖 (DIO)组 ,观察体脂含量的变化 ,放免法测血清生长激素含量 ,RT PCR法测定大鼠激素敏感脂肪酶mRNA水平。结果　DIO R大鼠体脂含量为 (4 2 6±0 5 1) % ,低于DIO大鼠的 (5 34± 0 34) % ;DIO R大鼠血清生长激素水平为 (0 70± 0 0 6 )ng/ml,高于DIO大鼠的 (0 5 0± 0 0 8)ng/ml;高脂饲料可增加DIO R大鼠白色脂肪激素敏感脂肪酶mRNA水平。结论　大鼠白色脂肪中激素敏感脂肪酶表达增加在大鼠肥胖抵抗的发生中起一定作用。