颗粒膜蛋白的表达与狼疮肾炎的关系研究

目的 了解血浆黏附分子颗粒膜蛋白（ＧＭＰ－１４０）水平及其在肾组织的表达与狼疮肾炎（ＬＮ）活动、肾脏病理及痛功能损害的关系。方法 用酶联免疫吸附法（ＥＬＩＳＡ）测定血浆ＧＭＰ－１４０水平,并用免疫组织化学染色法,观察正常肾和狼疮组织ＧＭＰ－１４０表达。结果 ＬＮ患者较正常人以及活动ＬＮ较非活动ＬＮ,血ＧＭＰ－１４０水平显著升高;ＬＮ肾组织ＧＭＰ－１４０表达较正常肾组织明显增强,且狼疮肾组织ＧＭＰ－     

抗菌勒管激素作为卵巢颗粒细胞瘤肿瘤标记物的实验研究

研究抗苗勒管激素（ａｎｔｉ－ｍｕｌｌｅｒｉａｎ ｈｏｒｍｏｎｅ,ＡＭＨ）,在诊断卵巢颗粒细胞瘤（ｇｒａｎｕｌｏｓａ ｃｅｌｌ ｔｕｍｏｒ,ＧＣＴ）中作为特异特性肿瘤标记的实验依据。方法采用免疫组织化学,原位杂交、Ｎｏｔｒｈｅｒｎ杂交法研究转基因小鼠早、晚期卵巢ＧＣＴ中ＡＭＨ及其受体ｍＲＮＡ的表达,并用放射性碘（＾１２５Ｉ）标记法,Ｎｏｒｈｔｅｎ杂交法研究原卵巢ＧＣＴ细胞中ＡＭＨ及其受体ｍＲＮＡ的表     

Endoscopic resection of colorectal granular cell tumors

AIM: To determine the feasibility and effectiveness of endoscopic resection for the treatment of colorectal granular cell tumors (GCTs).METHODS: This was a retrospective study performed at a single institution. From January 2008 to April 2015, we examined a total of 11 lesions in 11 patients who were treated by an endoscopic procedure for colorectal GCTs in the Endoscopy Center, Zhongshan Hospital of Fudan University, Shanghai, China. Either endoscopic mucosal resection or endoscopic submucosal dissection (ESD) was performed by three surgeons with expertise in endoscopic treatment. The pre- and post-operative condition and follow-up of these patients were evaluated by colonoscopy and endoscopic ultrasonography (EUS).RESULTS: Of these 11 lesions, 2 were located in the cecum, 3 were in the ileocecal junction, 5 were in the ascending colon, and 1 was in the rectum. The median maximum diameter of the tumors was 0.81 cm (range 0.4-1.2 cm). The en bloc rate was 100%, and the complete resection rate was 90.9% (10/11). Post-operative pathology in one patient showed a tumor at the cauterization margin. However, during ESD, this lesion was removed en bloc, and no tumor tissue was seen in the wound. No perforations or delayed perforations were observed and emergency surgery was not required for complications. All patients were followed up to May 2015, and none had recurrence, metastasis, or complaints of discomfort.CONCLUSION: Endoscopic treatment performed by endoscopists with sufficient experience appears to be feasible and effective for colorectal GCTs.     

Granular cell — Peripheral ameloblastoma: a rare variant

Ameloblastoma is an epithelial odontogenic tumour of the jaw and exhibits diverse microscopic patterns which occurs either singly or in combination with other patterns. Peripheral ameloblastoma is a rare odontogenic soft tissue tumor, derived from epithelial and/or mesenchymal elements being part of the tooth-forming apparatus. The lesions responsible for approximately 1% to 5% of all cases of ameloblastoma affecting alveolar mucosa and gingiva occurring mainly, in the middle age. This article describes a case of peripheral ameloblastoma affecting a 35-year-old female. The lesion was located in the right buccal space. The occurrence and pathogenesis of peripheral ameloblastomas in general, are reviewed. The tumour was excised and no recurrence has been observed after twenty four months of surgery.     

Granular cell tumour of tongue: A case report

Granular cell tumour, also known as Abrikossoff tumour, is a relatively uncommon benign neoplasm, which is more commonly found in females in the 4th to 6th decades of life even though it can occur in all ages.It occurs in all areas of the body but about 45–65% of all lesions are reported in the head and neck region. Intraoral lesions represent about 70% of the granular cell tumours of the head and neck, and account for 1/3 of all cases affecting the whole body.Most of the intraoral lesions occur on the tongue, usually on the lateral aspect, followed by the buccal mucosa and hard palate. Although majority of granular cell tumours are benign, some are clinically aggressive and a few frankly malignant forms have been reported. Some benign lesions exhibit surface ulcerations and this clinical appearance, combined with the overlying pseudoepitheliomatous hyperplasia, seen histologically, may lead to misdiagnosis of malignancy if adequate biopsy material was not taken.A case of granular cell tumour which occurred on the midline area of the dorsum of the posterior 1/3 of the tongue is reported in a 20-year-old female patient. It is interesting because the patient was younger than the average age of occurrence and the tumour occurred in a similar site to that of a lingual thyroid.     

Granular cell tumor mimicking a subglottic hemangioma

Pediatric airway tumors are uncommon. A 4-year-old girl with history of stridor since the first year of life underwent rigid laryngotracheal endoscopy revealing a left posterolateral subglottic mass occluding 80% of lumen. Complete tumor resection by open approach was undertaken. Histopathologic and immunohistochemical studies revealed granular cells tumor. The similarity of the clinical and endoscopic presentation of this tumor to the much more frequent subglottic hemangioma that usually requires a different therapeutic approach was of interest. Granular cell tumor must be considered in the differential diagnosis of upper airway obstructive symptoms in infancy and childhood.     

喉颗粒细胞瘤四例报告并文献复习

正颗粒细胞瘤(granular cell tumor,GCT)临床上较为少见,发生于喉部的更为罕见。2011年4月至2017年5月,青岛大学附属医院耳鼻咽喉头颈外科共收治喉颗粒细胞瘤4例,其中成人2例,儿童2例,现报告如下。1临床病例例1,男,41岁,因"声音嘶哑3年余"于2011年4月27日入院,检查:右侧声带中份可见息肉样肿物,表面光滑,声带活动可,闭合欠佳。电子喉镜检     

Tumeur à cellules granuleuses : à propos d’une localisation vulvaire compliquée de métastases pulmonaires

Granular cell tumour, or Abrikossoff's tumour, is a rare, ubiquitous tumour, with mostly benign evolution. The malignant forms represent 1–3% of the cases. The vulvar localization is exceptional. We report the case of a 28-year-old patient who presented a vulvar Abrikossoff's tumour. Initially considered benign, the tumour was treated by exclusive local surgery. Five months later, the tumour showed a malignant transformation. The tumour size was 14 cm in its major axis with the presence of not systematized scattered mitoses, PS 100 was positive at 100% and Ki 67 to 20%. Because of malignant and economic excision of the neoplasm, the patient received adjuvant radiotherapy. After 20 months, the patient had a local recurrence and lung metastasis, supporting the diagnosis of malignancy. She received two chemotherapy regimens of 5-fluoro-uracil/cisplatin and pacliatxel/carboplatin without any response. Following the failure of chemotherapy, the patient received symptomatic medical treatment. This observation leads to advocate an aggressive surgical treatment and strict supervision of all granular cell tumours.     

Morphologic changes in the granular convoluted tubule cells of the mouse submandibular gland following hypophysectomy and hormonal replacement

 Morphological changes in the granular convoluted tubule (GCT) cells of the male mouse submandibular gland (SMG) were examined following hypophysectomy and hormonal replacement. Semithin sections stained with Heidenhain's iron hematoxylin showed that hypophysectomy severely regressed the GCT phenotype. Although only a few dispersed cells containing secretory granules were observed in the GCT segments under a light microscope, electron microscopy revealed that many regressed cells continued to constitutively elaborate apical secretory granules (although they were very small) and contained a euchromatic nucleus at the center of the cell, poor rough endoplasmic reticulum (RER) and Golgi apparatus in the perinuclear region, and well-developed basal infoldings. These findings suggest that hypophysectomy resulted in atrophy of GCT cells, but that they retained evidence of being secretory cells. 5α-Dihydrotestosterone (DHT); 3,5,3′-triiodo-l-thyronine (T₃); and dexamethasone (Dex) each enhanced the GCT phenotype of hypophysectomized males to some degree. Combined hormonal replacement with DHT + T₃ in hypophysectomized males restored a nearly normal male GCT phenotype with a full complement of secretory granules and rare basal infoldings, whereas T₃ alone induced a normal female-like GCT phenotype, with considerably abundant secretory granules and the usual short basal infoldings, in hypophysectomized male glands. Furthermore, Dex was found to synergistically enlarge secretory granules when administered with T₃ and/or DHT, although it was only weakly effective in enhancing the GCT phenotype when used alone. Taken together, the above findings confirmed that the GCT phenotype of the mouse SMG is regulated by the synergistic action of pituitary-dependent hormones. Received: October 1, 2001 / Accepted: May 13, 2002     

Granular layer induction following the topical application of proliferating agents

Summary Evidence is provided for a possible dermal influence on the epidermis. Topical vitamin A stimulates a number of dermal cells with different enzyme reactions, and these invade the epidermis at about the time a granular layer is induced in mouse tail scale epidermis.N-hexadecane also induced a granular layer formation in the tail scale epidermis but the application of this agent only results in the invasion of the epidermis by non-specific esterase cells.These non-specific esterase cells are present in the follicular zone where a granular layer is usually present.It appears that dendritic cells may be responsible for the formation of a granular layer and that these cells in some way influence the keratinocytes to discharge their lyosomal enzymes and thus form a granular layer. It appears unlikely that the dendritic cells actually contribute their own acid hydrolases to the cell cytolysis necessary for the production of granular layer.