全文获取类型
收费全文 | 594篇 |
免费 | 16篇 |
国内免费 | 20篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 28篇 |
妇产科学 | 22篇 |
基础医学 | 101篇 |
口腔科学 | 11篇 |
临床医学 | 32篇 |
内科学 | 52篇 |
皮肤病学 | 5篇 |
神经病学 | 34篇 |
特种医学 | 9篇 |
外科学 | 50篇 |
综合类 | 72篇 |
预防医学 | 92篇 |
眼科学 | 9篇 |
药学 | 86篇 |
中国医学 | 7篇 |
肿瘤学 | 11篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 12篇 |
2021年 | 12篇 |
2020年 | 10篇 |
2019年 | 10篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 11篇 |
2015年 | 13篇 |
2014年 | 17篇 |
2013年 | 40篇 |
2012年 | 27篇 |
2011年 | 27篇 |
2010年 | 30篇 |
2009年 | 29篇 |
2008年 | 37篇 |
2007年 | 37篇 |
2006年 | 38篇 |
2005年 | 23篇 |
2004年 | 24篇 |
2003年 | 30篇 |
2002年 | 11篇 |
2001年 | 22篇 |
2000年 | 14篇 |
1999年 | 8篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 3篇 |
1995年 | 7篇 |
1994年 | 4篇 |
1993年 | 10篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 11篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1973年 | 2篇 |
1971年 | 1篇 |
排序方式: 共有630条查询结果,搜索用时 15 毫秒
91.
《Vaccine》2020,38(34):5480-5489
Malaria transmission blocking vaccines (TBV) target the sexual stage of the parasite and have been pursued as a stand-alone vaccine or for combination with pre-erythrocytic or blood stage vaccines. Our efforts to develop TBV focus primarily on two antigens, Pfs25 and Pfs230. Chemical conjugation of these poorly immunogenic antigens to carrier proteins enhances their immunogenicity, and conjugates of these antigens to Exoprotein A (EPA) are currently under evaluation in clinical trials. Nonetheless, more potent carriers may augment the immunogenicity of these antigens for a more efficacious vaccine; here, we evaluate a series of proteins to identify such a carrier. Pfs25 and Pfs230 were chemically conjugated to 4 different carriers [tetanus toxoid (TT), a recombinant fragment of tetanus toxin heavy chain (rTThc), recombinant CRM197 produced in Pseudomonas fluorescens (CRM197) or in E. coli (EcoCRM®)] and compared to EPA conjugates in mouse immunogenicity studies. Conjugates of each antigen formulated in Alhydrogel® elicited similar antibody titers but showed differences in functional activity. At a 0.5 µg dose, Pfs230 conjugated to TT, CRM197 and EcoCRM® showed significantly higher functional activity compared to EPA. When formulated with the more potent adjuvant GLA-LSQ, all 4 alternate conjugates induced higher antibody titers as well as increased functional activity compared to the EPA conjugate. IgG subclass analysis of Pfs230 conjugates showed no carrier-dependent differences in the IgG profile. While Alhydrogel® formulations induced a Th2 dominant immune response, GLA-LSQ formulations induced a mixed Th1/Th2 response. 相似文献
92.
目的探讨染色体异常在遗传咨询病人中的发生情况.方法取受检者外周血进行淋巴细胞培养,常规收获制片,G显带处理,显微镜下进行核型分析.结果在464例病人中共检出染色体异常24例,染色体异常检出率为5.17%,其中平衡易位3例(12.5%),罗伯逊易位3例(12.5%),倒位5例(20.83%),性染色体异常8例(33.33%),染色体不平衡易位5例(20.83%).结论妇产科及生殖内分泌许多疾病与染色体异常有关,对这些病人进行染色体检查很有必要. 相似文献
93.
94.
目的 分析2005—2020年玉溪市乙型病毒性肝炎流行特征和趋势,为今后乙型病毒性肝炎防制工作提供科学依据。方法 采用描述性流行病学方法,对玉溪市2005—2020年疾病监测信息报告管理系统报告的乙型病毒性肝炎和乙肝携带者疫情资料进行分析。结果 2005—2020年共报告乙肝病例11 721例,年均发病率为32.44/10万,16年间呈现下降趋势(χ2趋势=1 244.406,P=0.001)。乙肝携带者累计报告17 179例,年均报告率为46.59/10万,总体却呈现上升趋势(χ2趋势=824.577,P=0.002)。各县区乙肝病例年均报告发病率差异有统计学意义(χ2=26.214,P=0.011),居前3位的县区依次为华宁县54.57/10万、易门县41.37/10万和通海县40.36/10万。各县区乙肝携带者年均报告率差异有统计学意义(χ2=81.360,P=0.015),居前3位的县区依次为华宁县201.60/10万、峨山县75.03/10万和新... 相似文献
95.
Yoshihiro Shimauchi Masatoshi Tanaka Kenshi Koga Satoshi Itano Kunihide Ishii Ryukichi Kumashiro Shotaro Sakisaka Michio Sata 《Alcoholism, clinical and experimental research》2000,24(Z4):64S-67S
Background: We investigated the clinical characteristics of hepatitis C virus (HCV) antibody-positive hepatocellular carcinoma (HCC) patients who developed HCC at a relatively young age. Methods: Clinical characteristics of patients in their 40s were investigated and were compared with those of patients 50 years and older. The subjects were 648 HCC patients, 469 men (72%) and 179 women (28%), who were treated at our hospital between 1991 and 1997. Results: No patient was under 40 years of age. Eighteen patients (3%) were in their 40s, 137 patients (21%) were in their 50s, 338 patients (52%) were in their 60s, 143 patients (22%) were in their 70s, and 12 patients (2%) were in their 80s. Fifteen of the patients (83%) in their 40s were male. The proportion of men in their 40s was higher than that of all men. Eight of the 15 men in their 40s (53%) were heavy drinkers, and 2 (14%) were habitual drinkers. Three of the 15 men (20%) were HBV carriers, and these 3 HBV carriers were not drinkers. The proportion of heavy drinkers and HBV carriers was significantly higher among the patients in their 40s than in the 60 patients randomly sampled from the patients 50 years of age and older. The mean ages of male patients with HCC who were heavy drinkers, habitual drinkers, occasional drinkers, or nondrinkers were 52.3, 58.9, 62.0, and 61.7 years, respectively. HCC occurred significantly earlier in heavy drinkers than in the other 3 groups. We compared laboratory data of the patients in their 40s with data of all of the patients of 50 years and older. Serum total bilirubin, prothrombin time, and platelet counts were significantly worse in the patients in their 40s. Conclusions: Logistic regression analysis revealed that heavy drinking and presence of HBV infection were independently related to HCV antibody-positive HCC development at a younger age. 相似文献
96.
目的 探讨结合偶联半乳糖( Galactose , Gal) 抗 C D3 单克隆抗体( Anti C D3 Mc Ab) 肿瘤浸润性淋巴细胞( Tumorinfiltrating lymphocytes , T I L) 肝脏靶向性。 方法 本组把大鼠抗小鼠 C D 单克隆抗体和半乳糖偶联在一起,与3 H Td R 标记 T I L结合后,从鼠尾静脉注入小鼠体内,在注射不同时间眼眶取血05 ml,然后处死,切除肝、脾、肺,分别称重后进行放射性强度测定。 结果 结果显示:注射 Galanti C D3 Mc Ab T I L小鼠肝脏比注射单纯 T I L 的小鼠肝脏具有较高的放射性( P< 001) ,且该放射性持续较长一段时间,而脾、肺、血液内放射性强度差异无显著意义( P> 005 , P> 005 , P> 005) 。结论 该结果提示: Galanti C D3 Mc Ab T I L较单纯 T I L具有更好的肝脏靶向性。 相似文献
97.
聚乳酸和无活性脱钙骨基质载体对rhBMP-2异位诱导骨形成的比较性研究 总被引:1,自引:0,他引:1
目的:为了研究聚乳酸和无活性脱钙骨基质载体对rhBMP-2异位诱导的骨形成。方法:将重组人骨形态发生蛋白-2(rhBMP-2)与无活性脱钙骨基质(DBM)和聚乳酸(PLA)复合,植入小鼠股部外侧肌肉陷窝比较PLA和DBM载体对rhBMP-2诱导骨形成的影响。通过组织学检查、碱性磷酸酶(ALP)和钙含量测定以及骨钙素(BGP)放疫测定,进行对比观察。结果:术后21天,实验组均可见新生软骨细胞和软骨岛,rhBMP-2/PLA和DBM/rhBMP-2组还可见部分骨组织形成,见骨小梁、板层结构和红骨髓,rhBMP-2/PLA组PLA部分吸收,DBM/rhBMP-2组DBM无吸收迹象。各实验组与对照组对比,ALP、BGP水平和Ca量均有显著性差异(P<0.05),rhBMP-2/PLA与DBM/rhBMP-2组在ALP和BGP水平上相比无显著差异(P>0.05),但前组比后组的Ca量为高(P<0.05),这二组与单独植入rhBMP-2组相比,定量检测指标均有显著性差异(P<0.05)。结论:(1)PLA和DBM均为BMP的良好载体;(2)rhBMP-2/PLA是一种新的有价值的骨修复材料 相似文献
98.
99.
100.
目的:利用HIV病毒TAT蛋白转导结构域、人缺氧诱导因子1α氧依赖降解结构域以及CASPASE3构建融合蛋白的原核表达载体,并在大肠杆菌中表达及纯化。方法:分别构建原核表达质粒pET-28a(+)-TAT、pET-28a(+)-TAT-ODD、pET-28a(+)-TAT-ODD-CASPASE3(pET-28a(+)-TOP3);在大肠杆菌BL21(DE3)pLysS内,利用镍-亚硝胺乙酸-组氨酸(Ni-NTA-His)亲和层析法对重组蛋白TOP3进行纯化。结果:成功构建了pET-28a(+)-TOP3的原核表达载体,经过优化表达和纯化条件,融合蛋白在IPTG诱导下获得特异性表达,纯化得到了高纯度的目的蛋白。结论:融合蛋白TOP3的成功表达及纯化,为该融合蛋白应用于肿瘤的治疗研究奠定了理论基础。 相似文献