黄芪多糖对实验性阿弗他溃疡模型大鼠血清TNF-α的影响

目的：探讨TNF-α与复发性阿弗他溃疡（recurrent aphthous ulcer,RAU）发病机制的关系以及观察黄芪多糖（astragalus polysaccharide,APS）对RAU模型大鼠血清中TNF-α含量的影响。方法：通过免疫方法建立SD大鼠RAU动物模型,将大鼠随机分为5组：正常对照组（A）、阴性对照组（B）、APS低（C）、中（D）、高（E）剂量组。灌胃治疗20d后运用酶联免疫吸附试验（ELISA）检测各组SD大鼠血清中TNF-α的表达水平,并提取大鼠口腔黏膜做组织病理学观察。结果：阴性对照组（B）RAU大鼠血清中TNF-α的表达水平显著高于正常对照组（A）（P〈0.05）,连续灌胃20d后,APS各治疗组（C～E）大鼠血清中TNF-α的含量下降,较阴性对照组（B）有显著性差异（P〈0.05）,且高剂量（300mg/kg）APS对TNF-α表达水平的抑制作用较中（200mg/kg）、低（100mg/kg）剂量APS明显。结论：RAU发病可能与血清中TNF-α的表达异常有关,而体内应用APS可以通过剂量依赖方式有效地抑制RAU大鼠血清中TNF-α的表达。     

慢性前列腺炎患者前列腺液IL-1β和TNF-α的检测及意义

目的 :了解IL 1β和TNF α在慢性前列腺炎患者前列腺按摩液 (EPS)中的变化及临床意义。 　方法 :应用ELISA法对 34例慢性前列腺炎 [EPS中白细胞 (WBC)计数≥ 10 /HP为A组 16例 ,WBC <10 /HP为B组 18例 ]、10例无症状性前列腺炎、12例良性前列腺增生 (BPH)及 8例健康对照EPS中的IL 1β和TNF α进行检测。 　结果 :IL 1β和TNF α在EPS中WBC≥ 10 /HP的慢性前列腺炎和无症状性前列腺炎两组检测值明显高于WBC <10 /HP的慢性前列腺炎、BPH和健康对照 3组 (P <0 .0 5 ,P <0 .0 2 )。IL 1β与TNF α有显著的数列等级相关性 (P <0 .0 0 3) ,而WBC计数和IL 1β、TNF α之间的数列等级相关性无显著性意义。 　结论 :IL 1β与TNF α在伴有WBC计数增高的慢性前列腺炎患者EPS中明显增高 ,IL 1β与TNF α对传统以WBC计数为慢性前列腺炎进行分类的方法可以提供一个更准确的新分类方法。     

Generating fatty acid and vitamin D composition data of Indonesian foods

The Indonesian food composition table lacks data on individual fatty acids; data on vitamin D is incomplete. The primary aim of this study was to generate data on individual fatty acid content of Indonesian foods. Secondary objective was to analyze vitamin D content. Based on intake data of 4–12 year old children from a nationally representative Indonesian survey, 120 foods contributing 95% to children’s total polyunsaturated fatty acid intake were selected for chemical analysis of fat and fatty acid content. Vitamin D3 was analyzed in a subset of 60 foods. Food samples were collected throughout Indonesia; per food one representative composite sample was chemically analyzed. Of the analyzed foods, sardines (0.97 g/100 g), tempeh (0.62 g/100 g) and tofu (0.56 g/100 g) had the highest n-3 fatty acids content, peanuts (15.9 g/100 g) and palm oil (11.4 g/100 g) were richest in n-6 fatty acids. Vitamin D3 content in foods was limited. This paper significantly contributes to the current knowledge on the fatty acid and vitamin D content of a broad range of commonly consumed Indonesian foods, which can be used in future research to monitor dietary intake and guide policy makers and the food industry to steer nutrient intake in the Indonesian population.     

吡格列酮对2型糖尿病患者血清C反应蛋白和肿瘤坏死因子-α的影响

目的:观察吡格列酮治疗对2型糖尿病(T2DM)患者血清炎症因子C-反应蛋白(CRP)及肿瘤坏死因子-α(TNF-α)水平的影响.方法:采用随机、双盲法将2型糖尿病患者90例分为对照组和吡格列酮组,对照组联合应用磺脲类和双胍类药物,吡格列酮组每天加服吡格列酮15 mg治疗12周,分别检测两组治疗前后空腹血糖(FPG)、2 h血糖(2 hPG)、糖化血红蛋白(HbAlc)、血清CRP、TNF-α水平,评估治疗前后胰岛素抵抗(IR)变化.结果:对照组糖化血红蛋白(HbAlc)显著下降(P＜0.01),其他指标无显著变化;吡格列酮组治疗后FPG、2hPG、HbAlc、IR显著降低(P＜0.01),血清CRP、TNF-α水平治疗后显著下降(P＜0.01),与对照组比较差异有极显著性(P＜0.01).结论:吡格列酮治疗T2DM能改善胰岛素抵抗、降低血糖,具有明显的抗炎作用.     

Effects of ethanolic extracts of Euonymus alatus on CCl4-induced hepatic fibrosis in mice based on JAK2/STAT3 signaling pathway and its mechanism北大核心CSCD

Aim To explore the mechanism of ethanolic extracts of euonymus alatus on CCl4-induced hepatic fibrosis in mice by regulating JAK2/STAT3 signaling pathway. Methods Sixty C57BL/6J mice were randomly divided into control group,model group,EAL,EAM),EAH,and Silybin(n=10). Except for the control group,mice in other groups were injected with 25% CCl4 of 1.6 mL·kg-1 to induce HF model. Moreover,the positive group was administered 12.6 mg·kg-1 Silybin by gavage once a day,and EAL,EAM,and EAH were administered 72,140 and 280 mg·kg-1 ethanolic extracts of euonymus alatus once by gavage once a day,and the intervention lasted for six weeks. After 6th week,mouse blood was collected,the body weight and liver weight were measured and liver mass index calculated,the liver appearance was observed,and ALT,AST,TNF-α,IL-6,IL-1β were detected in serum. The protein expression levels of collagen Ⅰ,α-SMA,and JAK2/STAT3 signaling pathway-related protein(JAK2,STAT3,p-JAK2,pSTAT3)in mouse liver tissues were detected. Results Compared with the model group,EAM and EAH significantly decreased liver mass index,and the ALT,AST,α-SMA,collagen I levels of serum. After treatment,the liver morphology and structure,cellular inflammatory infiltration,fiber changes and collagen deposition in euonymus alatus intervention group were dose-dependently better than those of the model group. Compared with the model group,the expression of TNF-α,IL-6 and IL-1β in EAM and EAH serum decreased significantly(P<0.05). Compared with the model group,the protein expression levels of JAK2,STAT3,p-JAK2,pSTAT3 in EAM and EAH mouse liver tissues decreased significantly(P<0.05). Conclusion Ethanolic extracts of euonymus alatus have an anti-CCl4-induced HF effect in mice,and its mechanism may be related to the regulation of the JAK2/STAT3 signaling pathway. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

复方甘草酸苷对慢性乙型肝炎患者血清肿瘤坏死因子-α、白细胞介素-6的影响

目的:探讨复方甘草酸苷对慢性乙型肝炎患者血清细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的影响。方法:128例慢性乙型肝炎患者随机分为2组,对照组(62例)采用一般护肝治疗,治疗组(66例)在对照组基础上加用复方甘草酸苷治疗,疗程均为12wk。观察治疗前后2组患者血清TNF-α、IL-6水平变化。结果:治疗组肝功能恢复较快,血清TNF-α、IL-6水平显著降低,与对照组比较有显著性差异(P<0.05)。结论:复方甘草酸苷能显著降低慢性乙型肝炎患者血清炎性细胞因子TNF-α、IL-6水平,从而保护肝细胞。     

2种用药方案治疗慢性丙型肝炎的药物经济学评价

目的:评价2种用药方案治疗慢性丙型肝炎(CHC)的经济学效果。方法:185例CHC患者按照治疗方案的不同分为长效组(聚乙二醇干扰素α-2a联合利巴韦林)与普通组(普通干扰素α-2a联合利巴韦林),针对不同基因型分别采用最小成本分析法和成本-效果分析法进行研究,效果指标采用持续病毒学应答率(SVR),成本指标采用直接医疗服务成本。结果:对于基因2型、3型的CHC患者,长效组与普通组的SVR分别为76.5%、63.5%(P>0.05),治疗成本分别为30235.20、36950.40元(P<0.05);对于基因1型、未分型的CHC患者,长效组与普通组的SVR分别为52.9%、25.8%(P<0.05),成本-效果比分别为114310.77、143218.60,长效组相对于普通组的增量成本-效果比为86789.67。结论:从药物经济学角度看,聚乙二醇干扰素α-2a联合利巴韦林治疗CHC优于普通干扰素α-2a联合利巴韦林方案。     

Ki-67、TOPⅡα在食管鳞状细胞癌组织中的表达及其与脉管侵犯的相关性

目的:探讨食管鳞状细胞癌组织中Ki-67、TOPⅡα的表达及其与脉管侵犯的相关性。方法:回顾性分析60例食管鳞状细胞癌患者的临床资料,采用免疫组织化学法检测肿瘤组织中Ki-67、TOPⅡα的表达,比较有脉管侵犯者和无脉管侵犯者上述分子标志物表达的差异。结果:46例(76.7％)患者有脉管侵犯,其中在侵犯深度、TNM分期、淋巴结转移及组织学分级方面差异具有统计学意义(P<0.05)。有脉管侵犯者的TOPⅡα、Ki-67的高表达率显著高于无脉管侵犯者(P<0.05)。亚组分析显示,有和无脉管侵犯者之间Ki-67高表达率的差异主要出现于男性、年龄≥65岁、肿瘤最大径＜5 cm、组织学G2-G3级、浸润深度T3及TNM分期Ⅰ-Ⅱ期;有和无脉管侵犯者之间TOPⅡα高表达率的差异主要出现于年龄≥65岁、肿瘤最大径≥5 cm及组织学G2-G3级。Spearman相关性分析显示,Ki-67 和TOPⅡα 两者在食管鳞状细胞癌组织中表达呈正相关（r=0.314,P＝0.015）。结论:有脉管侵犯食管鳞状细胞癌患者的Ki-67、TOPⅡα的阳性表达率显著高于无脉管侵犯者,提示二者在脉管侵犯中起着重要作用。     

1α,25(OH)_2D_3对大鼠成骨细胞КB受体活化因子配体及骨保护素蛋白表达的影响

目的研究1α,25-二羟维生素D3(1α,25(OH)2D3)对体外培养3～4d的SD大鼠成骨细胞(OB)核因子κB受体活化因子配体(RANKL)及骨保护素(OPG)蛋白和mRNA表达的影响。方法1α,25(OH)2D3作用24、48、72h,分别采用ELISA及FQ-PCR法测定。结果10、100nmol/L1α,25(OH)2D3较对照及1nmol/L显著或极显著促进RANKL蛋白及mRNA表达;1、10nmol/L1α,25(OH)2D3较对照显著或极显著促进OPG蛋白及mRNA表达,而100nmol/L则极显著抑制OPGmRNA表达;RANKLmRNA/OPGmRNA及RANKL/OPG显示,1nmol/L组与对照组差异不显著,10、100nmol/L组RANKLmRNA/OPGmRNA及RANKL/OPG极显著高于对照组和1nmol/L组。结论低浓度1α,25(OH)2D3(1nmol/L)对骨更新作用不明显,而中、高浓度1α,25(OH)2D(310、100nmol/L)能通过上调RANKLmRNA/OPGmRNA及RANKL/OPG比例,促进OC的生成及骨吸收功能,增强骨更新。