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951.
L. K. Archibald O. Nwanyanwu P. N. Kazembe C. Mwansambo M. Bell H. Dobbie L. B. Reller W. R. Jarvis 《Clinical microbiology and infection》2003,9(3):234-238
Children in Malawi receive bacille Calmette-Guérin (BCG) vaccination within the first 3 days of life. Thus, we hypothesized that Malawian children infected with the human immunodeficiency type 1 virus (HIV-1) might be particularly vulnerable to dissemination of the BCG Mycobacterium bovis strain with which they were vaccinated. Following informed consent by parents, we studied children admitted to a Malawi general hospital during the 1998 wet and dry seasons. Blood from cohorts of acutely ill children was cultured for bacteria, including mycobacteria, and fungi, and tested for anti-HIV-1 antibodies. It was shown that non- typhi Salmonella and Escherichia coli were the predominant bloodstream pathogens during the wet and dry seasons, and that bloodstream dissemination of the BCG M. bovis strain is uncommon in HIV-1-infected children who receive the BCG vaccine. 相似文献
952.
JC virus genomes have been localized in formalin-fixed, paraffin-embedded brain tissues of two cases of known progressive multifocal leukoencephalopathy by in situ hybridization utilizing a biotinylated JC virus DNA probe. A three-stage immunoperoxidase system with gold-silver amplification of the diaminobenzidine substrate was used to visualize biotinylated nucleic acid hybrids. Dot-blot quantification of this visualization system indicates that subpicogramme amounts of biotinylated DNA can be detected. Optimal detection of the virus genomes in the brain tissues required a microwave irradiation step prior to hybridization. JC virus genomes were observed in the nuclei of enlarged oligodendrocytes and of some bizarre astrocytes. No other cell types were found to harbour the genomes. 相似文献
953.
Tetsuya Murata Shigeo Nakamura Hiroya Kato Yasushi Yatabe Taizo Shiraishi Makoto Kuroda Ryulchi Yatani Taizan Suchi 《Pathology international》1997,47(11):801-805
A case of Hodgkln's disease (HD), lymphocyte depression (LD) type In an Immunosuppressive patient is described. The patient was a 48-year-old male and his parents were born In the Kyushu area, which is an endemic area for adult T cell lymphomaheukemla (ATL). He was seropositive for ATL virus (ATLV, also referred to as HTLV-I) and showed a marked Immunosuppressive condition. He developed LD-HD and Pneumocystis carinii pneumonia, and died due to respiratory failure. The Immunohistochemical and in situ hybridization analyses revealed that the Reed-Sternberglike cells In the lymph node biopsy sample were positive for Ber-H2 (CD30), Leu-M1 (CD15), L-26 (CD20), Bcl-2, p53 and EBER, the viral genome of Epstein-Barr virus (EBV). 相似文献
954.
乙肝疫苗免疫失败儿童病毒S基因"a"决定簇变异研究 总被引:4,自引:0,他引:4
王永忠 《中国优生与遗传杂志》2003,11(4):29-30
目的 探讨江苏常州地区乙型肝炎疫苗免疫失败儿童病毒S基因“a”决定簇的变异情况。方法 对15例乙肝疫苗接种后血清表面抗原(HBsAg)阳性的儿童,采用聚合酶链反应方法(PCR)扩增其血清中HBV DNA S基因区。并对PCR产物直接标记测序。结果 15例HBsAg阳性儿童有14例血清HBV DNA阳性,其中有4例出现了S基因“a”决定簇的变异,变异率为28.6%,第126位的异亮氨酸(Ile)被苏氨酸(Thr)替代1例,第134位苯丙氨酸(Phe)被异亮氨酸替代1例。第145位甘氨酸(Gly)被丙氨酸(Pha)替代2例。结论乙型肝炎疫苗免疫失败儿童中存在s基因“a”抗原决定簇变异,江苏常州地区存在HBVS基因“a”决定簇变异的新类型。 相似文献
955.
956.
丙型肝炎病毒NS3蛋白单克隆杂交瘤细胞株的建立及初步鉴定 总被引:2,自引:1,他引:2
应用国产基因工程表达的丙型肝炎病毒(HCV)NS3区抗原免疫小鼠,然后取其脾细胞与小鼠骨髓瘤细胞系SP2/0融合,筛选出4株稳定分泌抗HCVNS3区蛋白单克隆抗体(McAb)的杂交瘤细胞株,分别命名为2B6,2F3,3D8,3D9,经初步研究表明这4株单抗与NS3抗原具有良好的反应性,与HCV核心区多肽及乙型肝炎病毒表面抗原和e抗原均无反应。抑制实验表明这4株抗体分别针对NS3抗原分子上的2个不同的抗原决定簇。 相似文献
957.
单克隆抗体间接荧光法分型检测单纯疱疹病毒的实验研究 总被引:2,自引:0,他引:2
本文选择3株抗单纯疱疹病毒型共同性和型特异性单克隆抗体,建立了分型检测单纯疱疹病毒的单克隆抗体间接荧光法(McAb-1FA).特异性试验和重复性试验证实,McAb-IFA特异性强、重复性好.用此法检测了不同部位来源的51份临床分离株,分型检测结果与ELISA法检测结果一致.提示McAb-IFA有可能成为实验室分型检测单纯疱疹病毒可靠的方法. 相似文献
958.
M Pezzella M A Rosci M Miceli N Vonesch E Sturchio M Mirolo E Mannella 《Journal of medical virology》1991,35(1):14-18
Twenty regular sexual partners of HIV-1 infected subjects, without detectable human immunodeficiency virus (HIV-1) antibody and positive for HIV-1 genome by in situ hybridization (ISH), were selected and studied longitudinally for 6-36 months to estimate the duration of silent infection. During the follow-up period, 10 showed atypical Western Blot (WB) patterns. Two seronegative partners seroconverted. Rapid progress to AIDS was observed in 7 seropositive subjects. 相似文献
959.
对柯萨奇B4病毒(CVB4)衣壳蛋白VPl保守区的亲水性和二级结构进行分析和预测,选择可能代表优势抗原表位的肽段进行合成(VPl-l肽,RIYF KPKHVK AYV),并截取了该肽段的前12个残基(VPI-2肽)用同样方法进行合成.用VP1-1肽免疫家兔制备出高效价抗体,应用酶联免疫吸附法(ELISA)检测,这些抗体与CVBI-6病毒均有结合反应,VP1-1肽与型特异性CVB1-6抗体均有良好的结合反应,表明VP1-1肽是CVB的共同抗原表位.用VP1-1肽检测心肌炎患者血清CVBIgM抗体,阳性率在40%左右. 相似文献
960.
Puri Beena Nelson William Porter Kevin R. Henchal Erik A. Hayes Curtis G. 《Virus genes》1998,17(1):85-88
We have determined the complete nucleotide sequence and the deduced amino acid polypeptide sequence of the genome of a dengue-1
(DEN-1) virus strain isolated from a patient on Nauru in the Western Pacific in 1974 (West Pac 74). The complete genome is
10,735 nucleotides in length and contains a single long open reading frame of 10,176 nucleotides encoding a polyprotein of
3392 amino acids. When compared to DEN-1 Singapore S275/90, the nucleotide and amino acid sequence homology are 94% and 97.8%,
respectively.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献