高效薄层层析法测定罗布麻烟中罗布麻浸膏的含量

利用高效薄层层析法,以罗布麻浸膏中的槲皮素为标准,测定罗布麻烟中罗布麻浸膏的含量。以甲苯:甲酸:乙酸乙酯:正己烷(2:0.2:1.5:0.2)作展开剂。可使得药烟中槲皮素与其它成分很好分离。分离后,用薄层扫描仪于波长410nm,参比波长750 nm扫描定量。本法需样品少,快速简便、灵敏准确。可适用于药烟生产厂家化验室检测使用。     

Immunohistochemical collagen analysis of the most superficial layer in adult articular cartilage

We investigated immunohistochemically the collagen type of the most superficial layer in 10 normal adult human articular cartilage specimens obtained from eight femoral heads and one each of the femoral condyle and the talus using routine light microscopy and polarizing microscopy. A membrane-like structure with strong bire-fringence covering the articular surface was observed under polarizing microscopy in each specimen. This structure was stained with anti-type I and anti-type III collagen antibodies but not with anti-type II collagen antibody. This immunohistochemical finding was identical to that in synovial tissue. The results of this study confirm that the most superficial layer of adult normal articular cartilage consists not of type II collagen but of types I and III, and that this layer is absolutely independent from its deeper layer.     

鼻中隔动脉的形态学观测

经两侧颈总动脉灌注墨汁混合液，显示鼻部动脉，借助体视显微镜对鼻中隔动脉各主干的数量，外径，长度，行程，分布范围及鼻中隔各区动脉主要来源，分支密度，吻合弓级数及弓数作了观测。     

原发性鼻腔非霍奇金淋巴瘤临床和病理分析(附17例报告)

目的：探讨原发性鼻腔非霍奇金淋巴瘤（NHL）的临床和病理特征。方法：对17例患者的临床资料进行回顾性分析。结果：17例鼻腔NHL中，NK/T细胞淋巴瘤9例，外周T细胞淋巴瘤6例，B细胞淋巴瘤2例。NK／T细胞淋巴瘤可见血管中心、血管浸润及坏死现象，外周T细胞淋巴瘤和B细胞淋巴瘤中末见。NK／T细胞淋巴瘤9例全部死亡，外周T细胞淋巴瘤6例中4例生存，B细胞淋巴瘤2例均生存。结论 原发性鼻腔NHL多发生于男性；NK／T细胞淋巴瘤较多见，具有较高的侵袭性和恶性度，预后更差。     

鼻部皮肤肿瘤切除后的创面修复

目的 介绍鼻部皮肤肿瘤切除后创面的即刻修复经验。方法 1996年4月～2006年4月，在遵循鼻亚单位组成的美学原则基础上，根据创面大小、形状、部位及周围皮肤情况，选择相应局部皮瓣修复鼻部肿瘤切除后致鼻缺损428例。其中男273例，女155例；年龄12～78岁，中位年龄52岁。基底细胞癌146例，鳞状细胞癌83例，表皮囊肿54例，色素痣145例；恶性肿瘤临床分期0～Ⅰ期，病程1周～3个月。鼻尖51例，鼻翼102例，鼻背138例，鼻侧壁137例；其中跨鼻亚单位缺损83例。肿瘤切除后致鼻缺损0．6cm×0．6cm～3．0cm×4．0cm。皮瓣来源于额鼻皮瓣58例，双叶皮瓣67例，改良菱形皮瓣152例，鼻唇沟皮瓣118例，额正中皮瓣33例。供区创面直接拉拢缝合。结果 患者创面Ⅰ期愈合423例；3例皮瓣远端部分表皮坏死，经局部换药后创面愈合；2例患者切口轻度感染，经局部换药处理后创面Ⅰ期愈合。供区均未形成继发性缺损，创面Ⅰ期愈合。术后385例获随访1～60个月。鼻外形轮廓较好，修复组织色泽、质地与周围皮肤匹配良好，恶性肿瘤无复发。结论 遵循鼻亚单位美学原则，应用局部皮瓣能修复鼻部肿瘤切除后中等以上创面，且色泽、质地、轮廓等均能达到较好匹配和协调，可获得满意功能和稳定的美学效果。     

上颌窦霉菌病的鼻内镜手术治疗

目的探讨鼻内镜下单纯上颌窦口扩大术治疗非侵袭性霉菌球型上颌窦炎的疗效. 方法回顾分析16例非侵袭性霉菌球型上颌窦炎的临床资料. 结果手术时间30～120 min,平均60 min.无手术并发症发生.住院5 ～7 d,平均6 d.16例术后病理找到菌丝和孢子,9例霉菌培养为曲霉菌.16例术后随访3～24个月,平均18个月,14例无症状,内镜示窦口通畅,窦腔粘膜正常;2例复发,再次扩大窦口,分别随访6、13个月无复发. 结论非侵袭性霉菌球型上颌窦炎鼻内镜下单纯上颌窦口扩大术可达到根治和微创的临床效果,但应特别注意窦口处理.     

食管胃Gambee单层吻合术

本文介绍了食管胃Gambee氏单层吻合方法,该方法具有单层、宽边、多保留粘膜及粘膜下层的特点,具有吻合口各层组织对合准确,血运丰富,吻合牢固,术野清楚,污染轻、操作简便等优点。经临床105例的应用,基本上能控制吻合口瘘及狭窄的发生。     

胎儿鼻粘膜毛细血管的超微结构研究

本文在透射电镜下观察了五例足月胎儿下鼻甲粘膜毛细血管的超微结构。在上皮下和腺泡周围的固有膜中观察到有孔毛细血管。我们对 Kanan 所提出的有孔毛细血管是出生后冷空气激发所致的观点提出疑议。     

Diagnostic significance of nasal eosinophilia in allergic rhinitis

Background  Nasal eosinophilia is one of the potential tests for substantiating the diagnosis of allergic rhinitis. Objective  The aim was to establish the validity of nasal eosinophilia in allergic rhinitis, to study it's various clinical correlates and interpret it in context of skin sensitivity pattern. Study Design  Prospective cased study. Setting  Hospital based. Patients  The patients were selected on the basis of history and clinical examination and were from the Himalayan region. Intervention  Diagnostic. Methods  The patients and the equal number of controls, were subjected to nasal smear for eosinophilia and intra-dermal skin tests to various allergeus. Results  Overall, eighty percent of nasal smears were positive in various degrees among the cases. Around eighty-eight percent of cases showed both smear and skin test positivity, thereby signifying a high degree of harmony among them and further validating and confirming the diagnosis of allergic rhinitis. Conclusion  Nasal eosinophilia was found to be a useful diagnostic test in allergic rhinitis, with a moderately high sensitivity and a high specificity.