Mutagenicity assessment of produced water during photoelectrocatalytic degradation

Oilfield produced water was treated by photocatalysis, electro-oxidation, and photoelectrocatalysis, respectively. The chemical composition and toxicity of the raw effluent and treated products were assessed by chemical and mutagenicity analysis. The raw effluent exhibited mutagenic activity in both strains of Salmonella typhimurium. The lowest concentration of the dichloromethane extract capable of inducing a positive response in strains TA98 and TA100 were as low as 4 and 5 microg/plate, respectively. All three technologies could detoxify direct-acting mutagenic organic pollutants efficiently, although they could not completely eliminate mutagenicity in the water after 60 min of treatment. At equivalent doses, photoelectrocatalysis exhibited the greatest capability to reduce genotoxicity, whereas photocatalysis was the least effective and did not cause appreciable change in mutagenicity. The gas chromatography-mass spectrometry (GC-MS) analysis revealed that n-alkanes (259.4 ng/L) and phenolic compounds (2,501.4 ng/L) were the main organic constituents in the oilfield produced water. Thus, the results of both biological and chemical analysis indicate that photoelectocatalysis was the most effective technology for degradation of oilfield wastewater.     

Preparation and protective efficacy of a chicken embryo kidney cell-attenuation GI-19/QX-like avian infectious bronchitis virus vaccine

Avian infectious bronchitis (IB) is a highly contagious disease, and hazardous to the poultry industry. Immune failure often occurs due to the emergence of new serotypes or field strains antigenically different from the vaccine strains. To prepare a candidate vaccine against the prevalent avian infectious bronchitis virus (IBV) in China, the GI-19/QX-like field isolate Sczy3 was selected as the progenitor strain and attenuated via passaging in chicken embryo kidney (CEK) cells for 100 times. The 100th generation of CEK-adapted strain, designated SczyC100, was safe to use on one-day old specific pathogen-free (SPF) chicken as determined by pathogenicity and virulence reversion test. The efficacies of SczyC100 and two commonly used commercial vaccines (H120 and 4/91) against prevalent GI-19/QX and GI-7/TWI type virulent strains were evaluated. Sczy3C100 effectively reduced the morbidity, mortality, mean lesion scores (MLSs), and viral load of trachea of chickens challenged by GI-19/QX and GI-7/TWI strains. CEK-adapted SczyC100 is therefore a potential vaccine candidate for the control of IB in China.     

Self-organization of hydroxyapatite nanorods through oriented attachment

The oriented attachment of hydroxyapatite (HAP) nanorods was achieved through a hydrothermal treatment. The nanorods were arranged in order along the c-axis of the HAP. These formed ca. 3.5 slip-shaped pores between the rods. High-resolution transmission electron (HRTEM) also revealed that single crystals of HAP may grow in the [0 0 2] direction of the HAP structure. This work extends the understanding of the potential for using biomimetic principles to synthesize bone-like composite materials.     

视网膜血管高血压交叉压迫征的量化研究

研究视网膜血管高血压交叉压迫征的量化方法,采用局部血管图像增强的匹配滤波进行预处理、数学形态学的闭合运算进行图像平滑、线化无级扩大法确定血管边界,实施管径可逆量化自动寻优算法,为获取相关疾病诊断的数据提供一种辅助手段。     

Prevention of entrance into G2 cell cycle phase by mimosine decreases locomotion of cells from the tumor cell line SW480

Cellular proliferation of tumor cells is thought to impede migratory activity. Using continuous single cell migration analysis of the colon carcinoma cell line SW480 for up to 72 h, we were able to show that cells locomote constantly and stop only for actual cell division. These findings indicate that proliferation (from G₁ phase to early mitosis) and migration do occur simultaneously. The presence of the cell cycle marker Ki-67 in individual migrating cells substantiated this observation. Inhibition of cell cycle progression by mimosine (MIM), a reversible cell cycle blocker, reduced the percentage of migrating cells; release from MIM block restored migratory capacity. The corresponding cell cycle phase distributions were confirmed by flow cytometry. In our test system cell cycle events and migration were shown to occur at the same time. Interference with cell cycle progression reduced migratory activity indicating that migration depends on an unhampered cell cycle.     

罗格列酮对2型糖尿病合并高血压患者的疗效

目的观察罗格列酮对2型糖尿病（T2DM）合并高血压（EH）患者的降血糖、降血压疗效以及改善胰岛素抵抗和尿微量白蛋白的作用。方法68例T2DM合并EH的患者随机分为治疗组和对照组,治疗组35例在服用二甲双胍和缬沙坦的基础上加用罗格列酮4 mg/d。对照组不加罗格列酮,疗程均为16周。比较治疗前后两组患者的空腹血糖（FBG）、餐后2 h血糖（PBG）、糖化血红蛋白（HbA1 c）、收缩压（SBP）、舒张压（DBP）、尿白蛋白排泄率（UAE）、空腹胰岛素（FINS）、胰岛素抵抗指数（IR I）,同时观察肝肾功能和不良反应。结果两组均能降低上述指标,但治疗组效果优于对照组,与对照组比较差异有统计学意义（P〈0.05）。结论罗格列酮对T2DM合并EH的患者有明显的降血糖、降血压、改善胰岛素抵抗和减少尿微量蛋白的作用,而且无明显不良反应。     

基于中国数字人电磁建模的手机天线辐射数值模拟

目的 旨在比较由于东西方人体结构差异导致手机天线在人体内的电磁辐射剂量变化。方法 应用基于数字化虚拟中国人数据集DVCH(Digitized Virtual Chinese Human)建立的人体电磁模型,采用时域有限差分法(FDTD)自主开发数值仿真软件。结果 比较了基于美国可视化人体模型VHP(Visible Human Project)和DVCH建模计算所产生的辐射剂量大小,并应用生物热方程计算人体内的温度升幅。结论 计算结果表明,两种人体模型内的SAR值均随距离增大而迅速衰减;比较而言,中国数字化人头模型给出的每克SAR平均值较大,而每克SAR峰值较小,这与DCVH模型体积略小于VHP模型有关;对人头内温度的数值模拟计算表明:手机辐射导致的稳态下最高温升约为0.25℃,引起脑内温度的升高在人体温度调节的正常范围内。     

学术不端文献的发表追溯及防范对策——基于185篇疑似学术不端文献的实证分析

目的 为学术期刊编辑及时、准确地辨别各种学术不端行为提供现实依据,以充分发挥编辑在防范和打击学术不端行为工作中的作用。方法 根据《中国学术期刊影响因子年报》个刊影响力统计分析数据库,调查《广东农业科学》2007—2013年发表疑似学术不端文献的数量、类型、年际变化和疑似源文献类型,深入分析2012—2013年不端文献的重合度、重合内容、产生历程以及作者特征等。结果 找出以上文献的“漏网”原因以及编辑部管理工作上的漏洞,发现学术不端行为已不是简单地表现为复制和拼凑,而是已经演化为隐式的深层次学术不端。结论 编辑部应严格规范审稿流程、强化责任编辑的学术把关职责、执行收稿时查重+发表前二次检测以及双系统检测、建立学术不端问题共享数据库、注重刊后学术不端文献的撤销处理。