临床药师参与依替米星致重症大疱型药疹治疗方案制定1例分析

目的 探讨临床药师在依替米星致患者重症大疱型药疹治疗过程中的作用。方法 临床药师对产生重症大疱型药疹可疑药物的关联性判断、抗感染方案的制定等方面提供建议,协助临床医师制定治疗方案。结果 医师采纳临床药师建议,治疗过程顺利,患者症状好转出院。结论 临床药师参与患者个体化用药方案的制定,促进了临床合理用药,确保患者用药安全。     

Oxygen radical generation by Maillard compounds

Amino sugars such as galactosamine are hepatotoxic. It has been verified that toxic hepatitis induced by galactosamine is similar to that of CCI₄ poisoning, and that both were inhibited by O₂¹ scavengers. Fructosamine results from the union of glucose with the ϵ-amine of lysine. A test for fructosamine quantification is based on nitroblue tetrazolium (NBT) reduction, in which O₂⁻ is involved, the reduction being inhibited in the presence of superoxide dismutase (SOD). Given these facts, we attempted to elucidate if galactosamine and glucosamine reduce NBT and if that reduction is inhibited by SOD. This was confirmed. Subsequently, we incubated aminoacids (glycine, lysine, alanine) with glucose and galactose for 7 days and studied the action of the incubation products on NBT, using amino acids and sugars as controls. We found that NBT reduction increases proportionally to the length of incubation time of glucose/galactose with lysine, but not with other amino acids. Reduction of NBT by the Amadori compounds formed is inhibited by SOD. We suggest that oxygen radical generation by Amadori compounds must be taken into consideration as one cause of damage in diabetes of long duration.     

Experimental Study on Strength Enhancement of Expansive Grout

Adding an expansive agent to ordinary grout can cause an expansion in volume, but also reduces its strength. In order to improve the strength of expansive grout, quartz sand is used as the strength enhancement additive. In this study, the expansion behavior and mechanical properties of the expansive grout with quartz sand are explored, through expansion development monitoring, uniaxial compression strength (UCS), acoustic emission (AE), SEM and XRD test methods. The results showed that: (1) The final expansion ratio and expansion development of the samples are related to the use of an expansive agent, but not affected by quartz sand. With the increase in expansion agent content, the average expansion ratios of the samples are 0.03%, 0.16%, 0.67%, 1.06% and 1.48%; (2) The UCS of the samples decreases with the increase in expansive agent content but increases with the increase in quartz sand content. Compared with no quartz sand, and with the increase in quartz sand content, the average strength of the samples increased by 10.51%, 29.88%, and 37.92%; (3) Quartz sand does not effectively participate in the hydration reaction, but it can effectively enhance the strength of the expansive grout without affecting its volume expansion, which makes it an ideal expansive grout strength enhancement additive.     

吲哚菁绿荧光导航在复杂腹腔镜胆囊切除术中的应用

目的:探讨吲哚菁绿荧光导航在复杂腹腔镜胆囊切除术(LC)中的临床价值。方法:回顾性分析2018年7月—2020年8月在鄂东医疗集团黄石市中心医院肝胆胰腺外科收治的96例复杂胆囊结石伴胆囊炎患者行LC的临床资料,按照术前是否静脉注射吲哚菁绿荧光导航分为实验组( n＝44)和对照组( n＝52),实...     

四溴双酚A对小鼠免疫功能的影响

目的:探讨四溴双酚A(TBBPA)对小鼠免疫功能的影响。方法:通过给小鼠灌胃服用TBBPA粉末(2.5 mg/d)21 d,期间测定脾脏重量和生长指数、体外抗体形成细胞和腹腔巨噬细胞吞噬功能等,检测TBBPA对小鼠外周免疫器官脾脏生长、抗体生成及吞噬细胞功能的影响。结果:服用TBBPA组小鼠脾脏的重量、生长指数低于对照组(P0.05);服用TBBPA组小鼠巨噬细胞吞噬百分率为(25.8±2.1)%,吞噬指数为0.4±0.3,均显著低于对照组(40.4±2.1)%、0.9±0.3(均P0.05);服用TBBPA组小鼠1×106个脾细胞所含PFC数为223.4±19.2,显著低于对照组405.8±14.5(P0.01)。结论:TBBPA可致小鼠抗体介导的特异性免疫和吞噬细胞参与的非特异性免疫功能下降。     

长链非编码RNA MCM3AP-AS1在胆管癌细胞中的表达及其功能

背景与目的：长链非编码RNA MCM3AP-AS1 (MCM3AP-AS1)在原发性肝癌、乳腺癌及胶质母细胞瘤等多种肿瘤中发挥癌基因功能,然而MCM3AP-AS1在胆管癌中的表达、功能及作用机制尚知之甚少。因此,本研究观察MCM3AP-AS1在胆管癌细胞中的表达及其对细胞增殖和侵袭的影响,并初步探讨机制。方法：采用q RT-PCR法检测胆管癌细胞株（CCLP、RBE、9810、Hu CCT1）及人肝内胆管上皮细胞株（HIBEC）中MCM3AP-AS1的表达。将胆管癌细胞转染MCM3AP-AS1 si RNA后,以转染无义序列的胆管癌细胞为阴性对照,分别用MTT实验和Transwell实验检测细胞增殖与侵袭能力的变化,用Western blot法检测JAK/STAT3信号通路与上皮间质转化（EMT）相关蛋白表达的变化;最后,用JAK/STAT3通路激动剂白血病抑制因子（LIF）行功能拯救实验验证。结果：所有胆管癌细胞系中MCM3AP-AS1表达量均明显高于HIBEC （均P<0.05）。与阴性对照组比较,CCLP细胞转染MCM3AP-AS1 si RNA后增殖能力与侵袭能力均明显减...     

胰岛素短期强化治疗新诊断2型糖尿病56例临床分析

目的观察短期胰岛素强化治疗新诊断2型糖尿病患者的疗效。方法对56例新诊断2型糖尿病患者采用3餐前短效、睡前中效胰岛素1日4次强化治疗,2～3周使血糖控制在目标范围(FBG<7.0mmolL,2hPG<10.0mmolL)且稳定2周,分别应用饮食运动和药物(降糖药物或降糖药物联合胰岛素)治疗。比较治疗前、1年后FBG、2hPG、FINS、2hINS、HOMAβ、HOMAIR、TG、TC、BMI等指标的变化,以及1年后降糖方式与治疗时胰岛素用量、血糖达标时间、低血糖发生率。结果1年后FBG、2hPG、HOMAIR显著下降(P<0.01),2hINS、HOMAβ显著上升(分别为P<0.05和P<0.01),FINS、BMI、TG、TC差异无显著意义(P>0.05);治疗时胰岛素用量少、达标时间短的患者1年后仅饮食和运动即能控制血糖,但治疗时低血糖发生率高。结论胰岛素短期强化治疗能改善新诊断2型糖尿病患者胰岛素分泌缺陷和敏感性,为今后血糖控制奠定基础;强化治疗达到目标血糖难易程度可以预测后期血糖控制及降糖方式。     

莪术挥发油提取工艺试验研究

目的通过正交试验,优选莪术挥发油最佳提取工艺。方法采用水蒸气蒸馏法,考察莪术的粒度、提取时间、浸泡时间对挥发油得率的影响。结果提取的最佳条件为粉碎粒度20~40目,蒸馏8h,不浸泡。结论优选得到的工艺简单、稳定、可行。     

重症肝炎合并脑卒中CT表现（附五例报告）

本文报道了５例重症肝炎合并脑卒中的ＣＴ表现。经ＣＴ扫描证实为脑内血肿伴蛛网膜下腔积血１例，缺血性脑梗塞和出血性脑梗塞各２。并对其发病机制进行了探讨，认为与重症肝炎有一定关系。