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101.
目的:建立天王补心丸中梓醇含量的测定方法。方法:采用反相高效液相色谱法。色谱柱为Agilent-ZORBX SB-C18柱,流动相为乙腈-0.1%磷酸溶液(V/V)=1:99,流速为1.0mL.min-1,检测波长为210nm,进样量为20μL,柱温为40℃。结果:梓醇检测浓度在2.004~20.046μg.mL-1范围内与其峰面积积分值呈良好的线性关系(r=0.9993);平均加样回收率为99.9%,RSD=2.2%(n=9)。结论:该法简便、准确、重复性好,适用于天王补心丸的质量控制。 相似文献
102.
目的研究腔内超声检查与MRI检查对于肛瘘患者诊断的准确性,为临床该类疾病的诊断提供依据。方法对2009年12月—2011年12月该院入院治疗的100例肛瘘患者进行了研究,随机分为两组,各50例,实验组患者采用MRI进行检测,对照组患者采用肛管内超声检测,将两组患者的检测结果与手术结果进行比较。结果实验组患者检测肛瘘位置诊断准确率为100%,肛瘘内口判断准确率为100%,对照组对应的准确率为70%和74%,两组比较,差异有统计学意义(P<0.05)。结论采用MRI方法对肛瘘患者进行手术前检测具有很高的准确性,快速、无损伤,好于腔内超声,值得临床推广应用。 相似文献
103.
目的探讨糖尿病大鼠肾小管-间质Prohibifin的表达。方法24只雄性SD大鼠分为12只链脲佐菌素(SIZ)诱导的糖尿病大鼠模型组(D)和12只正常组(N),4、8周每组各处死大鼠6只,测量24h尿清蛋白排泄率、肾重/体重、血肌酐、血糖(BS)。肾组织Masson染色。免疫组化方法检测肾小管.间质Prohibitin的表达。结果4、8周时尿清蛋白的排泄率正常组分别显著低于模型组[(1.90±0.36)μg,/min VS(3.35±0.56)μg/min,P〈0.01;(1.73±0.36)μg/mVS(4.53±0.87)μg/min,P〈0.01];肾小管.间质Prohibifin的表达正常组明显高于模型组。模型组肾小管-间质的细胞外基质增多。结论糖尿病大鼠肾小管.间质Prohibitin的表达下调,与肾小管-间质损伤的严重程度呈正相关。 相似文献
104.
目的 探讨P2Y受体在鼻咽癌细胞中的表达及其与鼻咽癌转移之间的关系.方法 采用RT-PCP.检测P2Y受体在鼻咽癌细胞系中的表达,趋化试验分析P2Y受体与鼻咽转移的关系.结果 P2Y受体在7株鼻咽癌细胞中的表达有明显的差异,其中P2Y12在所有鼻咽癌细胞株中均无表达,而P2Y1和P2Y6仅弱表达于HNE2细胞中.在其他细胞株中不表达.HNE2细胞株几乎表达所有检测的P2Y受体,而HNE3细胞株仅表达P2Y11受体.趋化试验表明,鼻咽癌细胞能在ATP作用下发生明显移动,以ATP浓度为1.0×10-4mol/L时趋化作用最明显.其趋化指数(CI)为4.3±0.6,这种趋化作用是一种定向迁移,而非随机运动.结论 P2Y受体在鼻咽癌的转移中可能发挥着重要的作用. 相似文献
105.
秋季腹泻是小儿常见病.多由饮食不当和肠道内或肠道外感染引起,肠道内感染以致病性大肠杆菌及轮状病毒为多见,自2005年9月-2006年11月采用双黄连粉针剂联合金双歧活菌片治疗婴儿秋季腹泻57例,并与同期应用抗生素及病毒唑治疗的28例对照,效果显著,总有效率94.8%,现报道如下。 相似文献
106.
BACKGROUND: Multipotent adult progenitor cells (MAPCs) from the bone marrow have been shown to differentiate into neurons.
OBJECTIVE: To observe migration, survival, and neuronal-like differentiation of MAPCs by tail vein injection. DESIGN, TIME AND SETTING: Randomized, controlled experiment of neural tissue engineering was performed at the Laboratory for Cardio-Cerebrovascular Disease, Hospital of Integrated Traditional and Western Medicine, Tongji Medical College of Huazhong University of Science and Technology between September 2006 and August 2007. MATERIALS: Eighty Sprague Dawley rats, 3-6 months old, underwent cerebral ischemia/reperfusion by thread technique, and were randomly divided into model and MAPCs groups (n = 40). METHODS: Mononuclear cells were harvested from bone marrow using the FicolI-Paque density gradient centrifugation method. After removing CD45 and glycophorin A-positive cells (GLYA+) with immunomagnetic beads, CD45 GLYA adult progenitor cells were labeled with bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU). A total of 1 mL cell suspension, containing 5 × 10^6 MAPCs, was injected into the MAPCs group through the tail vein. A total of 1 mL normal saline was injected into the model rats.
MAIN OUTCOME MEASURES: After 60 days, BrdU and neuron-specific enolase double-positive cells were observed using immunofluorescence. Cell morphology was observed under electron microscopy, and nerve growth factor mRNA was measured through RT-PCR. In addition, rat neurological functions were measured with behavioral tests.
RESULTS: Immunofluorescence revealed that MAPCs positive for BrdU and neuron specific enolase were found surrounding the ischemic focus in the MAPCs group. Microscopic observation suggested that MAPCs-derived neuronal-like cells connected with other nerve cells to form synapses. Compared with the model animals, the level of nerve growth factor mRNA was significantly upregulated in rats injected with MAPCs (P 〈 0.05). In addition, rats in the MAPCs group performed better in behavioral tests than the model group on days 28 and 60 (P 〈 0.05).
CONCLUSION: Transplanted MAPCs migrated to the ischemic region, survived, and differentiated into neuronal-like cells, resulting in stimulation of nerve growth factor mRNA and improved neurological function in ischemic rats. 相似文献
OBJECTIVE: To observe migration, survival, and neuronal-like differentiation of MAPCs by tail vein injection. DESIGN, TIME AND SETTING: Randomized, controlled experiment of neural tissue engineering was performed at the Laboratory for Cardio-Cerebrovascular Disease, Hospital of Integrated Traditional and Western Medicine, Tongji Medical College of Huazhong University of Science and Technology between September 2006 and August 2007. MATERIALS: Eighty Sprague Dawley rats, 3-6 months old, underwent cerebral ischemia/reperfusion by thread technique, and were randomly divided into model and MAPCs groups (n = 40). METHODS: Mononuclear cells were harvested from bone marrow using the FicolI-Paque density gradient centrifugation method. After removing CD45 and glycophorin A-positive cells (GLYA+) with immunomagnetic beads, CD45 GLYA adult progenitor cells were labeled with bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU). A total of 1 mL cell suspension, containing 5 × 10^6 MAPCs, was injected into the MAPCs group through the tail vein. A total of 1 mL normal saline was injected into the model rats.
MAIN OUTCOME MEASURES: After 60 days, BrdU and neuron-specific enolase double-positive cells were observed using immunofluorescence. Cell morphology was observed under electron microscopy, and nerve growth factor mRNA was measured through RT-PCR. In addition, rat neurological functions were measured with behavioral tests.
RESULTS: Immunofluorescence revealed that MAPCs positive for BrdU and neuron specific enolase were found surrounding the ischemic focus in the MAPCs group. Microscopic observation suggested that MAPCs-derived neuronal-like cells connected with other nerve cells to form synapses. Compared with the model animals, the level of nerve growth factor mRNA was significantly upregulated in rats injected with MAPCs (P 〈 0.05). In addition, rats in the MAPCs group performed better in behavioral tests than the model group on days 28 and 60 (P 〈 0.05).
CONCLUSION: Transplanted MAPCs migrated to the ischemic region, survived, and differentiated into neuronal-like cells, resulting in stimulation of nerve growth factor mRNA and improved neurological function in ischemic rats. 相似文献
107.
目的研究襄樊市城区蝇类区系。方法收集分析己知蝇类的区系成分,并与湖北省的宜昌、荆州、武汉3城市蝇类区系进行比较。结果襄樊市区已知蝇类5科77种,其中属东洋界为主的种类30种,占40.5%;古北属界为主的种类25种,占33.8%;广布两界者19种,占25.7%。结论分析研究后认为襄樊市区蝇类区系应划归东洋界。湖北省4个城市蝇类区系成分区别不大。 相似文献
108.
二烯丙基二硫诱导人胃癌细胞G_2/M期阻滞中p-CDK1、cyclin B1和p21~(WAF1)蛋白的表达 总被引:2,自引:2,他引:0
二烯丙基二硫(diallyl disulfide, DADS)是大蒜中提取的一种低分子量的脂溶性成分,也是大蒜中的主要有效成分,对结肠癌、乳腺癌、肝癌、肺癌、膀胱癌、前列腺癌和白血病等多种肿瘤均有明显的抑制作用.本实验室以前研究表明[1],DADS对人类白血病细胞HL60有诱导分化作用,对体外[2]和体内[3]的人胃癌MGC803细胞均有生长抑制作用、G2/M期阻滞作用.对体内的人胃癌细胞有诱导分化作用[3],对体外的人胃癌细胞有诱导凋亡作用[4]. 相似文献
109.
前列腺癌是目前男性所面临的一个主要疾病,目前对前列腺特异性抗原(PSA)等研究的不断深入使得对前列腺癌的诊断和治疗有了新的认识。现结合近几年各协会提出的诊疗大纲及有关文献,就前列腺癌的诊断和治疗作一综述。 相似文献
110.
临床工程师在医疗器械风险管理中具有重要的地位和作用。我国临床工程师队伍的发展不容乐观,临床工程师的作用未得到重视。本文分别对政府管理部门、医疗机构、企业等提出建议,以期能促进临床工程师的发展,充分发挥其在我国医疗器械风险管理中的作用。 相似文献