Combined therapy of intensive statin plus intravenous rt-PA in acute ischemic stroke: the INSPIRE randomized clinical trial

Journal of Neurology - To investigate the safety and efficacy of intensive statin in the acute phase of ischemic stroke after intravenous thrombolysis therapy. A total of 310 stroke patients...     

腹腔镜肝左外叶切除治疗左肝外叶胆管结石的体会

目的探讨腹腔镜肝左外叶切除治疗肝左外叶胆管结石的方法及疗效。方法回顾性分析我科2005年5月-2010年5月共12例患者行腹腔镜肝左外叶切除术治疗肝左外叶胆管结石的临床资料,其中2例并发胆囊结石,术中加行腹腔镜胆囊切除术。结果 12例均在腹腔镜下顺利完成手术,术中出血70 mL～370 mL,术后12例均出现一过性转氨酶升高,3例出现肝残面积液,无残留结石、大出血、胆瘘、腹腔脏器损伤等严重并发症发生。结论腹腔镜肝左外叶切除治疗左肝外叶胆管结石是安全可行的。     

奥拉西坦治疗老年脑出血后认知功能障碍的疗效和安全性

目的评价奥拉西坦治疗老年脑出血后认知功能障碍的疗效和安全性。方法选择2018年4月~2019年5月我院收治的102例老年脑出血患者作为研究对象。用随机数表法分为对照组和研究组,每组51例。两组病人均实施常规额颞瓣开颅手术,术后对照组接受常规治疗,研究组加用奥拉西坦。比较两组患者的临床疗效。结果治疗后,研究组的蒙特利尔认知评估量表(MoCA)评分、简易精神状态量表(MMSE)评分均高于对照组(P<0.05);治疗后研究组的神经功能缺损量表(NIHSS)评分低于对照组(P<0.05);两组患者手术时间、术中出血量及血肿清除率相较,无统计学差异(P>0.05);研究组不良发生率(2例)低于对照组的7例(P<0.05)。结论老年脑出血患者术后给予奥拉西坦治疗,可改善其认知功能,而且不良反应少。     

Limitations of Body Surface Area–Based Activity Calculation for Radioembolization of Hepatic Metastases in Colorectal Cancer

PurposeTo calculate absorbed radiation doses in patients treated with resin microspheres prescribed by the body surface area (BSA) method and to analyze dose-response and toxicity relationships.Materials and MethodsA retrospective review was performed of 45 patients with colorectal carcinoma metastases who received single-session whole-liver resin microsphere radioembolization. Prescribed treatment activity was calculated using the BSA method. Liver volumes and whole-liver absorbed doses (D_WL) were calculated. D_WL was correlated with toxicity and radiographic and biochemical response.ResultsThe standard BSA-based administered activity (range, 0.85–2.58 GBq) did not correlate with D_WL (mean, 50.4 Gy; range, 29.8–74.7 Gy; r = −0.037; P = .809) because liver weight was highly variable (mean, 1.89 kg; range, 0.94–3.42 kg) and strongly correlated with D_WL (r = −0.724; P < .001) but was not accounted for in the BSA method. Patients with larger livers were relatively underdosed, and patients with smaller livers were relatively overdosed. Patients who received D_WL > 50 Gy experienced more toxicity and adverse events (> grade 2 liver toxicity, 46% vs 17%; P < .05) but also responded better to the treatment than patients who received D_WL< 50 Gy (disease control, 88% vs 24%; P < .01).ConclusionsUsing the standard BSA formula, the administered activity did not correlate with D_WL. Based on this short-term follow-up after salvage therapy in patients with late stage metastatic colorectal carcinoma, dose-response and dose-toxicity relationships support using a protocol based on liver volume rather than BSA to prescribe the administered activity.     

FAS−1377 A/G polymorphism in breast cancer: a meta-analysis

FAS is a cell surface receptor involved in apoptotic signaling in many cell types including cells of the immune system. The ?1377 A/G polymorphism of FAS gene has been detected in breast cancer. However, the published evidence regarding the ?1377 A/G polymorphism and breast cancer risk has generated controversial results. We performed a meta-analysis of five case–control association studies totaling to 5,995 study subjects including 2,905 cases and 3,090 controls. The combined odd ratios (ORs) with its 95 % CIs were used to assess the association of the ?1377 A/G polymorphism correlated with breast cancer susceptibility with the fixed-effects model. The combined results showed significantly increased risk associated with the ?1377 A/G polymorphism under AA vs. GG genetic model (OR?=?1.28, 95 % CI?=?1.04–1.58; heterogeneity test: P?=?0.614, I ² ?=?0.0 %), AA vs. GA + GG genetic model (OR?=?1.24, 95 CI?=?1.02–1.51; heterogeneity test: P?=?0.349, I ² ?=?10.0 %), and allele model A vs. G (OR?=?1.10, 95%CI?=?1.02–1.20; heterogeneity test: P?=?0.422, I ² ?=?0.0 %). Similarly, significant association was found in Asians. In stratified analyses by control source, a higher risk was indicated in the hospital-based studies rather than the population-based studies. This meta-analysis suggests that the ?1377 A/G polymorphism is likely to be associated with the risk of breast cancer, especially the A allele in Asians.     

Antimetastatic effects of licochalcone A on oral cancer via regulating metastasis-associated proteases

Licochalcone A, a major phenolic constituent of the licorice species Glycyrrhiza inflata, has been proven to possess various biological benefits including anti-cancer activity. However, the detailed effects and molecular mechanisms of licochalcone A on the invasiveness and metastasis of oral cancer cells have not been fully understood. Thus, SCC-25 oral cancer cells were subjected to a treatment with licochalcone A at indicated concentrations (25, 50, and 100 μg/mL) for 36 h and then analyzed for the effect of licochalcone A on the cell migration and invasion. In vitro assays, including wound healing, cell adhesion, and cell invasion/migration assays, revealed that licochalcone A treatment significantly inhibited the cell migration/invasion capacities of SCC-25 cells. Also, results of zymography and Western blotting showed that activity and protein level of matrix metalloproteinase-2 (MMP-2) was suppressed, but TIMP-2 level was increased, indicating the important role of MMP-2 and TIPM-2 in anti-metastatic regulation of SCC-25 cells. Furthermore, licochalcone A was shown to suppress the nuclear factor-kappa B (NF-κB) signal, as evidenced by the decreased expression of phosphorylated p65 (p-65) protein in licochalcone A-treated SCC-25 cells. Notably, we also found that licochalcone A treatment increased the expression of the epithelial marker E-cadherin and decreased the expression of mesenchymal markers N-cadherin in SCC-25 cells. This is the first report describing the effects and possible mechanisms of licochalcone A on tumor invasion and metastasis of SCC-25 cells. Taken together, our findings support that licochalcone A can be developed to a potent anti-metastatic candidate for oral cancer therapy.     

不同浓度海藻酸盐凝胶的力学特性及其对软骨细胞增殖能力的影响

细胞外基质的理化性能影响着细胞的命运并调节组织形成。为探索最佳的软骨细胞体外三维生长环境,本研究通过改变藻酸盐浓度测定了海藻酸盐小球的力学特性及其对软骨细胞增殖能力的影响。凝胶的压缩机械性能通过INSTRON3365测定,结果发现浓度越高其压缩模量越大,表明凝胶对抗应变的能力越强。同时选取4w龄乳兔关节软骨细胞分别包裹在浓度为1%、2%和3%(w/v)海藻酸盐小球中连续培养4周,结果发现所有3组软骨细胞都表现出有限的增殖并形成类似于软骨组织的细胞簇,其中较低浓度组(1%和2%)增殖更明显。这些结果表明软骨细胞在海藻酸盐凝胶小球中连续培养有少量增殖,且较低浓度的藻酸盐更有利于软骨细胞的增殖,说明藻酸盐凝胶的力学特性影响了软骨细胞的增殖能力。     

水囊压迫止血对脑出血术后颅内压的影响

目的观察脑出血术后血肿腔置水囊压迫止血对颅内压的影响。方法选择15例脑出血病人,清除颅内血肿后血肿腔置水囊压迫止血,同时行侧脑室钻孔脑室外引流,引流管外接三通后接颅内压监测仪,并于术中及术后3d连续监测颅内压,根据颅内压变化情况给予脱水治疗。于术后1d、3d行头部CT复查,了解脑水肿及再出血情况。结果术中颅内压正常14例,轻度增高1例;术后1d正常10例,轻度增高4例,中度增高1例;术后2d正常8例,轻度增高5例,中度增高2例;术后3d正常5例,轻度增高6例,中度增高3例,重度增高1例。结论颅内血肿术后水囊少量注水压迫血肿腔止血,对术后颅内压影响小,安全可靠。     

间充质干细胞和Stro-1+亚群免疫抑制的比较及IDO1、IDO2在其中的作用

目的:比较间充质干细胞(MSC)和经Stro-1抗体分选的Stro-1+ MSC对淋巴细胞增殖反应的影响;探讨吲哚胺2,3二氧化酶1(IDO1)和IDO2在上述两种间充质干细胞免疫调节功能中的作用.方法:从全髋关节置换术患者的近端股骨粉碎物中得到细胞悬液,培养MSC,并筛选Stro- 1+MSC.体外设立单向混合淋巴细胞反应(MLR)体系,分别加入1×103～5× 104个经照射的Stro-1 +MSC或MSC,检测对T淋巴细胞增殖反应的影响.再分别加入IDO1和IDO2的特异性抑制剂L-1MT和D-1MT,检测T 淋巴细胞增殖反应的变化;以实时定量PCR法比较IDO1、IDO2在StRO-1+MSC和MSC中的表达,以GAPDH作为内参照基因.结果:(1)不同剂量组,Stro- 1+MSC对MLR的抑制效应均显著强于相同剂量的未经分选的MSC (P＜0.05); (2)加入L-IMT后,Stro-1+MSC和MSC对MLR的抑制作用均显著减弱(P＜0.05),而加入D-1MT后Stro-1+MSC和MSC对MLR的抑制作用与未加入抑制剂的对照组相比无显著变化(P＞0.05);(3)加入L-1MT后,Stro-1+MSC和MSC对MLR抑制的显著差异不复存在(P＞0.05),加入D-1MT后Stro-1+MSC对MLR的抑制作用仍显著强于未经分选的MSC(P＜0.05);(4)实时定量PCR检测发现IDO1在Stro-1+MSC中的表达显著高于MSC (P＜0.05),而IDO2的表达在两者间无差异(P＞0.05).结论:IDO1在MSC的免疫调节和Stro-1+MSC的强势抑制功能中发挥重要作用,该作用可被IDO1的特异性抑制剂L-1MT所抑制,而IDO2的特异性抑制剂D-1MT无此作用.     

驻海南某部海军官兵健康状况调查

目的了解海军官兵的健康状况及影响因素。方法采用整群抽样方法,用自行设计的健康调查表对648名海军官兵进行问卷调查。调查表含有与精神、循环及消化系统疾病相关的20项亚健康状态自觉症状。结果发生率位列前5项的自觉症状分别为烦躁（70.1%）、乏力（65.1%）、情绪低落（61.7%）、失眠（61.0%）、头晕（58.3%）;不同服役期的官兵组间烦躁、情绪低落、失眠发生率差异有统计学意义（P〈0.05）;干部、战士头晕的发生率以及不同文化程度官兵失眠的发生率的组间差异有统计学意义（P〈0.05）。结论本组被调查官兵均存在不同程度的健康问题,与服役年限、学历、任职有一定关系。