Significance of serum IgA in patients with acute hepatitis E virus infection

AIM: To study the significance of serum anti-hepatitis E virus (HEV) IgA in patients with hepatitis E. METHODS: A new method was established to assay anti-HEV IgA, which could be detected in the middle phase of the infection. We compared anti-HEV IgA assay with anti-HEV IgM and anti-HEV IgG assay in sera from 60 patients with positive HEV-RNA. RESULTS: The 60 patients with positive HEV-RNA had both anti-HEV IgA and anti-HEV IgM and 410 patients with negative HEV-RNA were used as control. Periodic serum samples obtained from 60 patients with hepatitis E were tested for HEV RNA, anti-HEV IgM, anti-HEV IgA and anti-HEV IgG. Their HEV-RNA was detectable in the serum until 20±11 d. We used anti-HEV IgM and anti-HEV IgA assay to detect HEV infection and positive results were found in 90±15 d and 120±23 d respectively, the positive rate of anti-HEV IgA was higher than that of anti-HEV IgM and HEV-RNA (P <0.05). CONCLUSION: The duration of anti-HEV IgA in serum is longer than that of anti-HEV IgM, and anti-HEV IgA assay is a good method to detect HEV infection.     

坚持以患者为中心,积极稳妥推进按病种付费

从博弈论的视角出发,以维护患者利益,构建和谐医患关系为目标,提出了保证按病种付费顺利有效实施的具体措施:医疗保险机构应以患者为中心,完善按病种付费实施方案;医疗机构应以患者为中心,积极配合按病种付费推行;加强宣传,营造按病种付费的良好氛围。     

A novel indole alkaloid from deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth

To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their ¹H and ¹³C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test.     

梅毒血清试验阳性的女性不孕症分析

目的探讨梅毒血清试验阳性对女性不孕症患者妊娠和胎儿的影响。方法分析在我院生殖中心就诊的女性不孕症患者的临床资料,随访梅毒血清试验阳性者妊娠及新生儿情况。结果梅毒血清试验阳性者139例(1.38%),RPR阳性/TPPA阴性35例;RPR阴性/TPPA阳性11例;RPR与TPPA均阳性93例。经驱梅治疗后,RPR阳性/TPPA阴性23例;RPR阴性/TPPA阳性7例;RPR与TPPA均阳性4例。74例患者通过辅助生殖成功怀孕,69例分娩出正常新生儿,5例在孕期。结论女性不孕症患者梅毒血清试验阳性率较高;梅毒血清试验阳性的女性不孕症者,通过规范驱梅或试验性治疗后,可进行辅助生殖且与梅毒血清试验阴性患者分娩的新生儿无明显差异。     

基于《丹溪心法·痰》探讨肝癌发病与痰的相关性

肝癌的治疗目前对人类来说仍是一个难以攻克的难题。基于《丹溪心法·痰》对中医病理产物"痰"的认识与阐述,探讨肝癌与痰的病因病机相关性。通过阐述痰的本质、肝癌的病因病机与痰的关系论证肝癌与痰的相关性。并结合文献,对我国三个肝癌高发区致病危险因素的中医辨证进行分析,进一步阐明痰在肝癌发病中的重要促进作用,以期对肝癌的临床治疗发挥指导作用,重视肝癌从痰论治。     

乌拉尔甘草中黄酮类化学成分的研究

目的 研究产自内蒙古杭锦旗的乌拉尔甘草Glycyrrhiza uralensis 根中的黄酮类成分。方法 采用硅胶、聚酰胺、MCI、ODS、Sephadex LH-20、RP-HPLC等色谱方法分离纯化,根据理化性质和波谱数据进行结构鉴定。结果 从乌拉尔甘草根95%、70%乙醇提取物中分离得到19个化合物,分别鉴定为3-甲基山柰酚（1）、染料木素（2）、柚皮素（3）、甘草异黄酮甲（4）、甘草利酮（5）、异芒柄花素（6）、半甘草异黄酮B（7）、3′-异戊烯基染料木素（8）、黄羽扇豆魏特酮（9）、甘草黄酮醇（10）、黄宝石羽扇豆素（11）、甘草异黄酮乙（12）、异甘草黄酮醇（13）、甘草宁H（14）、粗毛甘草素 A（15）、甘草西定（16）、粗毛甘草素D（17）、香豌豆酚（18）、7-甲基羽扇豆异黄酮（19）。结论 化合物1、6、8、18、19为首次从甘草属植物中分离得到,化合物2～4、12、14、15为首次从该种植物中分离得到。