磷脂酰肌醇3-激酶在血管新生相关性疾病中的作用

血管新生参与了人体多种生理与病理过程,前者如创伤愈合,后者包括肿瘤、动脉粥样硬化(atherosclerosis,AS)、类风湿性关节炎(rheumatoid arthritis,RA)等多种疾病,血管新生与这些疾病的发生发展密切相关.磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase, PI3K)作为细胞内重要的信号蛋白,可介导细胞迁移、增殖与血管新生等多种生物学行为的信号转导.     

五倍子牙膏对釉质矿化作用的影响

目的:评价五倍子牙膏抑制釉质脱矿和促进人工早期釉质龋再矿化的作用.方法:检测五倍子牙膏处理离体牙前后乳酸盐缓冲液中磷离子浓度的变化,计算出牙釉质溶解度降低率(enamel soly rate,ESR),评价五倍子牙膏抑制釉质脱矿作用;制作人工龋釉质块模型,体外药物pH循环,检测五倍子牙膏处理前后釉质表面显微硬度恢复率(...     

5个新查耳酮类化合物的设计及合成

目的 设计、合成新结构类型的查耳酮类化合物,为新药设计和开发提供有价值的信息。方法 根据关于具有抗肿瘤活性的查耳酮类化合物构效关系的初步研究及第三代芳香维甲类的经典构效关系研究,设计并合成了查耳酮类新化合物,并对目标化合物进行人白血病细胞株HL-60细胞毒活性筛选,结合筛选结果,采用SYBYL 7.3软件对所合成化合物进行能量优化及构象分析。结果 设计并合成了5个未见报道的查耳酮类新化合物,均经光谱方法鉴定了结构。所有化合物均对HL-60细胞呈现不同程度的细胞毒活性,同时能量优化得到最低能量构象并进行了构象分析。结论 本实验为新的查耳酮类化合物的设计提示了信息与依据。     

慢性肾脏病病人自我管理量表编制的研究

[目的]编制适用于慢性肾脏病病人的自我管理量表。[方法]运用查阅文献及专家评定的方法编制慢性肾脏病病人自我管理量表,采用随机抽样的方法,对120例慢性肾脏病病人进行调查,并于1周后随机抽取其中的30例病人进行重测,考察量表的信度和效度。[结果]量表的总体Cronbach’sα系数为0.902,重测信度为0.898,专家对量表总体评定的内容指数为0.901,各维度与总体的相关系数为0.684～0.878,因子分析采用主成分分析法,以特征值〉1强制提取4个公因子,累积贡献率为49.293%。[结论]慢性肾脏病病人自我管理量表具有较好的信度和效度,适用于了解慢性肾脏病病人的自我管理水平。     

Relation Between Red Cell Distribution Width With Echocardiographic Parameters in Patients With Acute Heart Failure

BackgroundRed cell distribution width (RDW) has recently been discovered to be a novel prognostic marker in patients with heart failure. However, the relation between RDW and echocardiographic parameters in acute heart failure (AHF) has not been studied.Methods and ResultsWe analyzed laboratory findings including RDW, N-terminal pro-brain natriuretic peptide (NT-proBNP), and echocardiographic parameters in 100 patients with AHF. The mean RDW was 14.2 ± 2.0% and median NT-proBNP was 5183 pg/mL. The mean left ventricular ejection fraction was 33.1 ± 14.5% and early mitral inflow velocity to early diastolic mitral annular velocity (E/E′), was 21.2 ± 9.4. When the RDW was considered in tertile categories, the highest tertile group (>14.5%) had higher E/E′ (P < .001) and higher NT-proBNP (P = .02) than the lowest tertile group (<13.2%). In multiple linear regression analysis, RDW was independently correlated with E/E′ even after adjustment of other risk factors (β-coefficient 0.431, P = .001). The optimal cutoff value of RDW for predicting E/E′ >15, suggesting elevated left ventricular filling pressure (LVFP) was 13.45% (area under the curve 0.633, P < .05). An additive power of RDW with NT-proBNP for predicting E/E′ >15 was found in logistic regression analysis (P = .038).ConclusionsWe found a novel relation between higher levels of RDW and elevated E/E′ in patients with AHF. This novel finding raises the possibility that a simple marker, RDW may be associated with elevated LVFP in patients with AHF.     

核糖体蛋白S24基因在胃癌及其癌前病变中差异表达的研究

背景与目的:将胃癌组织、正常胃黏膜及胃癌前病变的基因表达进行比较,找出与人胃癌密切相关的基因片段,为进一步探讨胃癌的发生机制、胃癌的早期诊断和治疗方案提供重要的理论根据。材料与方法:应用荧光mRNA差异显示技术(FluorescentmRNAdifferentialdisplay,FDD)分析3例胃癌、3例正常胃粘膜、3例胃癌前病变组织,分析基因差异表达情况。分离差异表达基因片段,进行PCR扩增。将cDNA片段测序,测序结果提交Genbank,经BLAST软件检索与同源性分析。选取其中差异表达的核糖体蛋白S24(RibosomalproteinS24,RPS24)基因,应用Northern杂交进行验证,并进一步对RPS24基因进行基因表达系列分析和组织表达谱分析。结果:mRNA差异显示发现RPS24基因在胃癌组织中的表达明显高于癌前病变和正常胃黏膜组织,并且Northern印迹验证阳性。基因表达系列分析和组织表达谱分析发现RPS24基因在多种肿瘤组织中表达增高以及分布广泛。结论:与非癌组织相比,RPS24基因在胃癌中表达明显增高,RPS24的高表达可能与胃癌的发生、恶性进展有关。     

Reconstruction of orbital defects by implantation of antigen-free bovine cancellous bone scaffold combined with bone marrow mesenchymal stem cells in rats

Background

Tissue-engineering approach can result in significant bone regeneration. We aimed to reconstruct the segmental orbital rim defects with antigen-free bovine cancellous bone (BCB) scaffolds combined with bone marrow mesenchymal stem cells (BMSCs) in rats.

Methods

BCB was prepared by degreasing, deproteinization and partly decalcification. BMSCs isolated from green fluorescent protein (GFP) transgenic rats were osteogenically induced and seeded onto BCB scaffolds to construct induced BMSCs/BCB composites. An 8-mm full-thickness defect on the rat inferior-orbit rim was established. Induced BMSCs/BCB composites cultured for 5 days were implanted into the orbital defects as the experimental group. Noninduced BMSCs/BCB group, BCB group and exclusive group were set. General condition, spiral CT, 3D orbital reconstruction, histological and histomorphometric analysis were performed after implantation.

Results

BCB presented reticular porous structure. GFP-BMSCs adhering to BCB appeared bright green fluorescence and grew vigorously. Infection and graft dislocation were not observed. In induced BMSCs/BCB group, CT and 3D reconstruction showed perfect orbital repair situation. Histological analysis indicated BCB was mostly biodegraded; newly formed bone and complete synostosis were observed. The percentage of newly formed bone was (57.12?±?6.28) %. In contrast, more residual BCB, less newly formed bone and nonunion were observed in the noninduced BMSCs/BCB group. Slowly absorbed BCB enwrapped by fibrous connective tissue and a small amount of new bone occurred in BCB group. Fibrous connective tissue appeared in exclusive group.

Conclusions

Antigen-free bovine cancellous bone that retains natural bone porous structure and moderate mechanical strength with elimination of antigen is the ideal carrier for mesenchymal stem cells in vitro. BCB combined with BMSCs is a promising composite for tissue engineering, and can effectively reconstruct the orbit rim defects in rats.     

国内临终关怀模式研究的文献计量分析

[目的]分析国内关于临终关怀模式的文献,揭示我国现有临终关怀模式的特点及存在的问题.[方法]以"模式"为主题词,检索1988年-2010年中国期刊全文数据库(CNKI),以"临终"为主题词进行二次检索,下载文献并汇总分析.[结果]共检索文献211篇,逐篇阅读筛选出符合要求的文献26篇.其中近5年发表文献16篇(61.5%),项目基金课题文献5篇(19.2%),广东地区文献发表量居首位,论文最高引用频次为21次.[结论]我国对临终关怀模式的研究尚处于探索阶段,构建科学的临终关怀模式以克服现有模式的不足将有助于括导今后临终关怀工作的开展.     

社区中老年人骨质疏松症相关知识与自我效能调查研究

[目的]了解聊城社区中老年人对骨质疏松症(OP)知识的认知及自我效能情况,为健康教育的顺利实施提供依据。[方法]采用便利取样法对聊城社区110名中老年人,应用OP相关健康行为问卷及骨质疏松自我效能量表进行调查。[结果]聊城社区110名中老年人骨质疏松症知识得分较低,不同学历及职业之间的得分差异均有统计学意义(P<0.05),不同文化程度、职业、钙摄入量组之间自我效能差异有统计学意义(P<0.05)。[结论]聊城社区中老年人骨质疏松症知识知晓率有待提高,需进一步开展骨质疏松症知识的健康教育,以提高社区中老年人的自我效能水平。     

护生临床实习不同时期压力源和应对方式的量性与质性研究

[目的]通过量性和质性相结合的方法对实习护生在实习不同阶段的压力源及应对方式进行调查。[方法]采取目的抽样的方法,将天津医科大学护理学院2005级本科护理专业、在天津市内医院实习护生纳入研究,应用压力源量表和简易应对问卷,在实习不同时期进行问卷调查,并从中选取8名护生进行焦点团体访谈。[结果]实习前压力水平最高,随着实习时间的延长,总体呈现递减的趋势,但均处于中等及以上水平,压力源排序前3位的为对知识和技能的需求、监督与评价、教学安排;实习护生积极与消极应对随实习变化趋势不对应,出现较大波动。[结论]实习护生压力随实习时间延长而减低,但面对众多压力源;且应对方式并未随实习时间延长而呈现积极应对持续升高。