太原市某卷烟厂噪声对作业工人心血管系统的影响调查

[目的]探讨噪声对作业工人心功能的影响,制定更合理的防护措施。[方法]2008年5月。对太原市某卷烟厂125名噪声作业工人进行心脏健康监护。并与对照比较。[结果]高血压患病率噪声组125人为10．40％,对照组125人为6．40％（P〉0．05）;心电图异常率,噪声组为47．20％,对照组为20．80％（P〈0．01）。[结论]本次调查调查对象的样本量较少,有待扩大样本量进一步确定噪声对卷烟厂作业工人心血管系统的影响程度。     

1997～2006年子长县乙型病毒性肝炎疫情资料分析

[目的]了解子长县乙型病毒性肝炎（乙肝）发病情况及流行病学特征,评价防治工作效果,为制定防治策略提供依据。[方法]对1997-2006年乙肝疫情资料进行分析。[结果]1997-2006年子长县累积报告乙肝病人638例,年均发病率为27.22/10万,呈逐年上升趋势。年均发病率,男性为34.25/10万,女性为19.63/10万（P〈0.01）;0-9岁为17.41/10万,10-19岁为29.37/10万,20-39岁为32.23/10万,40-59岁27.59/10万,60岁以上为19.18/10万（P〈0.01）;农村为29.08/10万,城区为22.57/10万（P〈0.01）。638例病人中,农民占47.96%。[结论]子长县乙肝疫情呈大幅度上升趋势,大年龄组儿童和青壮年是高发人群。     

潼关县一起流感疫情暴发的调查分析

目的分析潼关县流感疫情暴发的流行病学特征,为防控流感等呼吸系统传染病提供经验。方法对临床诊断病例进行个案调查,采集发病初期未用药患者的鼻、咽拭子进行实验室检测和病毒培养,对收集的资料和流行过程进行流行病学分析。结果潼关县2所中小学共发生流感样病例42例,罹患率为2.19%;经对15例发病初期未用药患者鼻、咽拭子标本进行检测,11份甲型流感病毒抗原呈阳性;RT-PCR检测,甲型流感病毒核酸呈阳性,阳性率为73.30%。结论此次流感暴发疫情的病原体为甲型流感病毒,提示2008年流感流行病原体很有可能将以甲型为主。     

白藜芦醇联合大豆异黄酮对衰老模型大鼠学习记忆能力及葡萄糖转运蛋白表达的影响

目的探讨白藜芦醇联合大豆异黄酮干预对衰老模型大鼠回避记忆能力及海马组织神经元特异性核蛋白(neuron-specific nuclear protein, NeuN)阳性细胞数、葡萄糖转运蛋白(glucose transporter, GLUT)1及GLUT3基因和蛋白表达的影响。方法 60只SD雌性大鼠按体重随机分为6组:假手术组、衰老模型组、80 mg/kg白藜芦醇组、160 mg/kg大豆异黄酮组、80 mg/kg白藜芦醇+160 mg/kg大豆异黄酮组、0.8 mg/kg戊酸雌二醇阳性对照组。采用去卵巢合并腹腔注射100 mg/kg D-半乳糖制备衰老模型。干预组灌胃给予受试物,每天1次,持续12周。穿梭箱实验检测大鼠主、被动回避能力,免疫荧光染色检测NeuN阳性细胞数,实时荧光定量PCR和免疫印迹法分别检测海马GLUT1、GLUT3基因及蛋白水平。结果与假手术组相比,模型组大鼠回避潜伏时间延长,主动回避反应率及总回避反应率降低,NeuN阳性细胞数减少,GLUT1和GLUT3基因及蛋白表达水平下调(P<0.05)。与模型组相比,3个干预组大鼠回避潜伏时间明显缩短(P<...     

西安市售红富士苹果中的六六六

目的建立测定苹果中六六六和滴滴涕农药残留量的气相色谱分析方法,测定西安市售的红富士苹果中的六六六和滴滴涕残留量。方法苹果捣碎用乙腈提取,盐析,用氮吹仪吹干,正己烷溶解,Florisil柱净化,滤液再次氮吹近干,正己烷定容,采用DB-1毛细管柱分离,ECD检测器测定。结果 6种六六六和滴滴涕能较好地分离,峰面积与其浓度均具有良好线性,3个水平的加标回收率在90.0%～106.1%之间,相对标准偏差均小于8.0%。采集的20份样品中,六六六和滴滴涕的残留量均符合国家标准要求。结论该方法简便,快速,灵敏,准确,可以满足苹果中六六六滴滴涕农药检测的需要。     

放射作业人员淋巴细胞微核检出结果

目的探讨应用外周血淋巴细胞微核的检测进行慢性小剂量电离辐射生物学效应剂量估算的实际意义。方法查阅太钢放射作业人员的健康档案,选出有确切暴露剂量的放射作业人员为研究对象,选用某福利企业非放射作业人员的体检资料作为对照组,按不同工龄组、不同性别组以及吸烟组,采用SPSS软件进行统计分析。结果射线暴露组与对照组的微核检出率之间的差异有统计学意义,射线暴露的不同工龄组的微核检出率之间的差异也有统计学意义;两性别组的微核检出率之间的差异无统计学意义;吸烟者的微核检出率均高于不吸烟者,差异有统计学意义。结论淋巴细胞微核率与小剂量电离辐射的累积剂量的大小之间存在着剂量—反应关系;性别对放射作业人员淋巴细胞微核率的检测结果无影响;吸烟是诱发外周血淋巴细胞产生微核的危险因素之一。     

吕梁市离石区对糖尿病患者进行社区护理干预的效果评价

[目的]探讨对2型糖尿病患者进行社区护理干预,对于改变患者不良生活方式与生活习惯,改善血糖及相关血生化指标的效果。[方法]2009年8月至2010年12月,对吕梁市离石区疾病预防控制中心纳入基本公共卫生项目管理的340例2型糖尿病患者（进行社区护理干预的干预组175例、对照组165例）进行生活方式护理干预研究,并分析其效果。[结果]综合干预1年后,干预组的不良生活方式纠正率均较干预前明显提高,干预后干预组均明显高于对照组（P〈0.01或〈0.05）;空腹血糖、餐后2h血糖、糖化血红蛋白、甘油三酯、总胆固醇,干预后2组均低于干预前,干预后干预组均低于对照组（P〈0.01）。[结论]对糖尿病患者进行社区护理干预,使患者的不良生活方式得到转变,血糖等指标降低。     

Immunogenicity and safety of an inactivated SARS-CoV-2 vaccine (Sinopharm BBIBP-CorV) coadministered with quadrivalent split-virion inactivated influenza vaccine and 23-valent pneumococcal polysaccharide vaccine in China: A multicentre,non-inferiority,open-label,randomised, controlled,phase 4 trial

BackgroundThe safety and immunogenicity of the coadministration of an inactivated SARS-CoV-2 vaccine (Sinopharm BBIBP-CorV), quadrivalent split-virion inactivated influenza vaccine (IIV4), and 23-valent pneumococcal polysaccharide vaccine (PPV23) in adults in China is unknown.MethodsIn this open-label, non-inferiority, randomised controlled trial, participants aged ≥ 18 years were recruited from the community. Individuals were eligible if they had no history of SARS-CoV-2 vaccine or any pneumonia vaccine and had not received an influenza vaccine during the 2020–21 influenza season. Eligible participants were randomly assigned (1:1:1), using block randomization stratified, to either: SARS-CoV-2 vaccine and IIV4 followed by SARS-CoV-2 vaccine and PPV23 (SARS-CoV-2 + IIV4/PPV23 group); two doses of SARS-CoV-2 vaccine (SARS-CoV-2 vaccine group); or IIV4 followed by PPV23 (IIV4/PPV23 group). Vaccines were administered 28 days apart, with blood samples taken on day 0 and day 28 before vaccination, and on day 56.ResultsBetween March 10 and March 15, 2021, 1152 participants were recruited and randomly assigned to three groups (384 per group). 1132 participants were included in the per-protocol population (375 in the SARS-CoV-2 + IIV4/PPV23 group, 380 in the SARS-CoV-2 vaccine group, and 377 in the IIV4/PPV23 group). The seroconversion rate (100 % vs 100 %) and GMT (159.13 vs 173.20; GMT ratio of 0.92 [95 % CI 0.83 to 1.02]) of SARS-CoV-2 neutralising antibodies in the SARS-CoV-2 + IIV4/PPV23 group was not inferior to those in the SARS-CoV-2 vaccine group. The SARS-CoV-2 + IIV4/PPV23 group was not inferior to the IIV4/PPV23 group in terms of seroconversion rates and GMT of influenza virus antibodies for all strains except for the seroconversion rate for the B/Yamagata strain. The SARS-CoV-2 + IIV4/PPV23 group was not inferior to the IIV4/PPV23 group regarding seroconversion rates and GMC of Streptococcus pneumoniae IgG antibodies specific to all serotypes. All vaccines were well tolerated.ConclusionsThe coadministration of the inactivated SARS-CoV-2 vaccine and IIV4/PPV23 is safe with satisfactory immunogenicity.This study is registered with ClinicalTrials.gov, NCT04790851.     

太原市某焦炉厂焦炉作业工人血清rasP21蛋白表达的5年随访研究

[目的]探讨rasP21蛋白在焦炉工血清中随接触时间的表达变化,以期用于焦炉工肺癌的早期筛选。[方法]2004~2008年对某煤气化公司焦化厂25名焦炉工连续进行血清rasP21蛋白检测,并与16名同集团公司的不接触职业性致癌物的库房管理人进行比较。[结果]2004~2008年血清P21水平,16名对照者有逐年增高的趋势(P<0.05),25名焦炉工均保持在较高水平(P>0.05);各年接触组均高于对照组(P<0.01或<0.05)。广义估计方程分析结果,分组、观察时间、起始年龄、饮茶(保护因素)与血清P21蛋白水平有统计学意义(P<0.01)。[结论]焦炉工血清P21高于对照组,随着时间的延长2组P21水平均越高,喝茶者血清P21水平较低。     

Nicotine increases apoptosis in HUVECs cultured in high glucose/high fat via Akt ubiquitination and degradation

It is well‐documented that nicotine, the main active ingredient in cigarettes, results in endothelial cell injury in numerous diseases. However, whether nicotine plays a crucial role in endothelial cell injury in diabetes and the exact molecular mechanism that mediates this process have not been fully elucidated. The current study aimed to investigate the effects of nicotine on endothelial cell injury in diabetes and the specific molecular mechanism by which it plays a role. Human umbilical vein endothelial cells (HUVECs) were incubated in HG/HF media and treated with nicotine, PYR‐41 (a selective ubiquitin E1 inhibitor), Akt‐overexpressing adenovirus, or TTC3 and MUL1 shRNA adenovirus. Cell viability was subsequently detected by the CCK8 assay, and apoptosis was examined by caspase‐3 cleavage and activity analysis. Compared to the HG/HF incubated group, nicotine incubation significantly decreased cell survival and increased apoptosis. Moreover, nicotine induced Akt degradation via UPS, and Akt overexpression blocked nicotine‐induced apoptosis in HUVECs cultured in HG/HF media. Furthermore, the TTC3 and MUL1 shRNA adenovirus dramatically decreased the Akt ubiquitination and apoptosis induced by nicotine. These results indicate that nicotine‐induced Akt ubiquitination and degradation occurs through TTC3 and MUL1 and results in a dramatic increase in apoptosis in HUVECs cultured in HG/HF media.