反式二羟环氧苯并芘对人支气管上皮细胞中HER2/neu基因表达的影响

目的探讨环境致癌物苯并(a)芘代谢物反式二羟环氧苯并芘(BPDE)对人支气管上皮细胞HER2/neu基因表达的影响。方法利用半定量RT-PCR、SYBR GreenI实时定量RT-PCR(QRT-PCR)、Western blot及免疫细胞化学方法分别检测经2.0μmol/L反式BPDE诱发人支气管上皮细胞恶性转化细胞(16HBE-T)与对照DMSO溶剂组未恶性转化细胞(16HBE-N)之间HER2/neu基因mRNA和蛋白表达水平的差异,及两组细胞内HER2/neu蛋白表达定位分析。结果经几种不同方法检测反式BPDE恶性转化人支气管上皮细胞中HER2/neu基因mRNA和蛋白水平都比对照溶剂组细胞(16HBE-N)表达显著升高(P<0.05)。HER2/neu蛋白定位在胞膜。结论反式BPDE诱发人支气管上皮细胞恶性转化存在HER2/neu表达增高,其可能与原癌基因HER2/neu被激活作用有关。     

Orally disintegrating olanzapine induces less weight gain in adolescents than standard oral tablets

We compared the changes in weight (kg) and body mass index (BMI) (kg/m²) in 52 hospitalized adolescents between baseline and after 12 weeks of monotherapy with either (i) olanzapine (OLZ) orally disintegrating tablets (ODT) (N = 16; 16.6 mg/day ± 4.4 [SD]), or (ii) OLZ standard oral tablets (SOT) (N = 10; 18.0 mg/day ± 4.2), or (iii) risperidone (N = 26; 2.8 mg/day ± 1.2). Significantly greater increases in mean weight and BMI were observed in the patients treated with OLZ SOT (8.9 ± 5.1 [SD] kg; 1.9 ± 0.6 kg/m², respectively) than in those with ODT (3.0 ± 2.1 kg; 1.1 ± 0.8 kg/m²). Similarly, OLZ ODT treatment was associated with significantly greater increases in weight and BMI than risperidone (1.0 ± 1.8 kg; 0.4 ± 0.7 kg/m²). These findings suggest that adolescents gain less weight with OLZ ODT than OLZ SOT, possibly because the former formulation shortens the time of interaction with digestive serotonin receptors mediating satiety.     

Pertussis in practice in 2007]

Universal vaccination of infants against pertussis has transformed the epidemiology of the disease. Pertussis has however become frequent, although not often diagnosed, in adolescents and adults and thus contributes to permanent transmission of Bordetella pertussis in France and contamination of young infants at risk of severe disease. Control of transmission of pertussis in France necessitates reinforcement of vaccination with late boosters in adolescents and adults and, in addition, education of physicians to recognize and treat early cases of pertussis, especially in adolescents and adults with a persistent or chronic cough, and to take appropriate prophylactic measures (antibiotics and recall vaccination) of those in contact with confirmed cases. Effective treatment does little to reduce symptoms but it does reduce transmission. Macrolides are the recommended treatment for pertussis.